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1. 发明申请

US20100285463A1 SELECTING NUCLEIC ACID SAMPLES SUITABLE FOR GENOTYPING 审中-公开
标题翻译：选择适合基因的核酸样品
公开(公告)号：US20100285463A1
公开(公告)日：2010-11-11
申请号：US12667135
申请日：2008-06-27
申请人： Florian Kronenberg , Veit Schoenborn , Iris Heid , Anita Brandstätter
发明人： Florian Kronenberg , Veit Schoenborn , Iris Heid , Anita Brandstätter
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2531/149 , C12Q2525/179
摘要： The present invention relates to a method for selecting a DNA sample comprising genomic DNA suitable for genotyping, comprising the steps of: i) pre-genotyping said genomic DNA using a set of polymorphic markers; ii) determining out of said set of polymorphic markers the percentage of polymorphic markers for which said genomic DNA is homozygous; and iii) selecting said DNA sample when said genomic DNA is homozygous for less than 70% of said set of polymorphic markers. Furthermore, a method of genotyping comprising a step of using a DNA sample selected by the method in accordance with the present invention and/or a step of applying the method provided herein is disclosed. The present invention also relates to a method for identifying a gene or a locus on a genome, said method comprising a step of using a DNA sample selected by the method provided herein and a step of applying the method described herein. Further, the present invention relates to a kit for carrying out the method in accordance with the present invention comprising primers for the amplification of the set of polymorphic markers employed herein.
摘要翻译：本发明涉及一种用于选择包含适合基因分型的基因组DNA的DNA样品的方法，其包括以下步骤：i）使用一组多态性标记对所述基因组DNA进行前基因分型; ii）确定所述多态性标记集合中所述基因组DNA是纯合的多态标志物的百分比; 以及iii）当所述基因组DNA对所述多态性标记物组的少于70％是纯合的时，选择所述DNA样品。此外，公开了一种基因分型方法，其包括使用根据本发明的方法选择的DNA样品和/或应用本文提供的方法的步骤。本发明还涉及用于鉴定基因组上的基因或基因座的方法，所述方法包括使用通过本文提供的方法选择的DNA样品的步骤和应用本文所述方法的步骤。此外，本发明涉及用于实施根据本发明的方法的试剂盒，其包含用于扩增本文使用的多态性标记组的引物。

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