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2. 发明申请

US20030087233A1 Methods, compositions and apparatus for making nucleic acid molecules having a selected affinity to a target molecule 审中-公开
标题翻译：用于制备对靶分子具有选择亲和性的核酸分子的方法，组合物和装置
公开(公告)号：US20030087233A1
公开(公告)日：2003-05-08
申请号：US09752231
申请日：2000-12-29
申请人： Q-RNA, Inc.
发明人： Abraham Grossman
IPC分类号： C12Q001/68 , C12P019/34 , C12M001/34
CPC分类号： C12N15/1058
摘要： The present invention is directed to methods, compositions, kits and devices for making a nucleic acid having selected affinity to a target molecule. One embodiment of the present invention is a method of making a replicatable nucleic acid template having a selected affinity to a target. The method comprises the step of applying a selection to a first generation comprising at least one replicatable nucleic acid template as the replicatable nucleic acid template is replicated by a nucleic acid polymerase to form at least one subsequent generation comprising a replicatable nucleic acid template. The selection is based on the affinity of the replicatable nucleic template of different generations to the target. The nucleic acid polymerase introduces genetic variability between generations of the replicatable nucleic acid template to produce replicatable nucleic acid templates having different affinities to the target. The replicatable nucleic acid templates are separated on the basis of the affinity of the replicatable nucleic acid template to the target.
摘要翻译：本发明涉及用于制备对靶分子具有选择亲和性的核酸的方法，组合物，试剂盒和装置。本发明的一个实施方案是制备具有对靶的亲和力的可复制核酸模板的方法。该方法包括将选择应用于包含至少一个可复制核酸模板的第一代，因为可复制核酸模板由核酸聚合酶复制以形成包含可复制核酸模板的至少一个随后的一代。该选择基于不同世代的可复制核酸模板对靶标的亲和力。核酸聚合酶在可复制的核酸模板的世代之间引入遗传变异性以产生与靶具有不同亲和力的可复制的核酸模板。可复制的核酸模板基于可重复核酸模板与靶标的亲和力而分离。

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