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1. 发明授权

US08821816B2 Matrix-assisted laser desorption ionization mass spectrometry substrates having low volume matrix array elements 有权
标题翻译：具有低体积矩阵阵列元素的矩阵辅助激光解吸电离质谱基质
公开(公告)号：US08821816B2
公开(公告)日：2014-09-02
申请号：US12123378
申请日：2008-05-19
申请人： Daniel P. Little , Maryanne J. O'Donnell-Maloney , Charles R. Cantor , Hubert Köster
发明人： Daniel P. Little , Maryanne J. O'Donnell-Maloney , Charles R. Cantor , Hubert Köster
IPC分类号： G01N37/00 , H01J49/04 , B01L3/02
CPC分类号： H01J49/0418 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00378 , B01J2219/00387 , B01J2219/00468 , B01J2219/00497 , B01J2219/005 , B01J2219/00504 , B01J2219/00511 , B01J2219/0052 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00659 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01L3/0244 , B01L3/0262 , B01L3/0268 , C07B2200/11 , C07F9/2408 , C07F9/2429 , C07H21/00 , C40B40/06 , C40B40/10 , C40B60/14 , G01N35/1067 , G01N35/1074 , G01N2035/1037 , G01N2035/1069 , Y10T428/26 , Y10T428/31678 , Y10T428/31855 , Y10T436/119163 , Y10T436/24 , Y10T436/25 , Y10T436/2575
摘要： Provided herein are substrates for matrix-assisted laser-desorption ionization (MALDI) mass spectrometric analysis. Each spot includes 3-hydroxypicolinic acid matrix and no analyte.
摘要翻译：本文提供了用于基质辅助激光解吸电离（MALDI）质谱分析的底物。每个斑点包括3-羟基吡啶甲酸基质，无分析物。

2. 发明授权

US08818735B2 Methods for generating databases and databases for identifying polymorphic genetic markers 有权
标题翻译：用于生成用于鉴定多态性遗传标记的数据库和数据库的方法
公开(公告)号：US08818735B2
公开(公告)日：2014-08-26
申请号：US13536807
申请日：2012-06-28
申请人： Andreas Braun , Hubert Köster , Dirk Johannes Van Den Boom , Ping Yip , Charles Rodi , Liyan He , Norman Chiu , Christian Jurinke
发明人： Andreas Braun , Hubert Köster , Dirk Johannes Van Den Boom , Ping Yip , Charles Rodi , Liyan He , Norman Chiu , Christian Jurinke
IPC分类号： G06F19/10 , G06F19/00 , G01N24/00 , C12Q1/68
CPC分类号： C12Q1/6872 , C12Q1/6827 , C12Q1/6883 , C12Q2600/156 , Y10T436/24 , C12Q2561/125 , C12Q2565/627 , C12Q2521/301 , C12Q2535/125
摘要： Processes and methods for creating a database of genomic samples from healthy human donors, methods that use the database to identify and correlate polymorphic genetic markers and other markers with diseases and conditions are provided.
摘要翻译：提供了从健康人类供体创建基因组样本数据库的方法和方法，使用数据库识别多态遗传标记和其他标志物与疾病和病症相关联的方法。

3. 发明授权

US07803529B1 Solid phase sequencing of biopolymers 有权
标题翻译：生物聚合物的固相测序
公开(公告)号：US07803529B1
公开(公告)日：2010-09-28
申请号：US09395409
申请日：1999-09-14
申请人： Charles Cantor , Hubert Köster
发明人： Charles Cantor , Hubert Köster
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6872 , C12Q1/6874 , C12Q2565/501 , C12Q2531/113 , C12Q2521/525 , C12Q2565/627 , C12Q2531/119 , C12Q2523/301 , C12Q2531/137 , C12Q2521/301 , C12Q2531/149
摘要： This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.
摘要翻译：本发明涉及用于检测和测序靶核酸序列，批量修饰的核酸探针和可用于这些方法的探针阵列的方法，以及含有这些探针的试剂盒和系统。有用的方法包括将代表靶的互补或同源序列的核酸或核酸与核酸探针阵列进行杂交。这些探针包括在单链部分内的单链部分，任选的双链部分和可变序列。该组的杂交核酸的分子量可以通过质谱法测定，并且靶标的序列由片段的分子量确定。可以确定其序列的核酸包括生物样品中的DNA或RNA，例如患者活组织检查和环境样品。探针可以固定在固体支持物例如杂交芯片上以促进自动化分子量分析和靶序列的鉴定。

4. 发明授权

US07759065B2 Mass spectrometric methods for detecting mutations in a target nucleic acid 失效
标题翻译：用于检测靶核酸突变的质谱法
公开(公告)号：US07759065B2
公开(公告)日：2010-07-20
申请号：US12125857
申请日：2008-05-22
申请人： Hubert Köster
发明人： Hubert Köster
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6872 , C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6883 , C12Q1/706 , G01N35/1067 , H01J49/00 , Y10T436/143333 , Y10T436/24 , C12Q2565/627 , C12Q2537/113 , C12Q2535/113 , C12Q2521/325 , C12Q2535/131 , C12Q2525/107 , C12Q2523/107 , C12Q2523/319 , C12Q2565/625 , C12Q2535/125
摘要： Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided. In some embodiments, a process comprises: amplifying one or more target nucleic acid molecules from a biological sample; ionizing and volatilizing the amplified product; and analyzing a product by mass spectrometry to determine an observed molecular mass of the product.
摘要翻译：提供了用于检测特定核酸分子的快速且高度精确的基于质谱的方法，并且提供了分子中的突变。在一些实施方案中，方法包括：从生物样品中扩增一个或多个靶核酸分子; 电离和挥发扩增产物; 并通过质谱分析产物以确定产物的观察分子量。

5. 发明授权

US06589485B2 Solid support for mass spectrometry 有权
标题翻译：固体支持质谱
公开(公告)号：US06589485B2
公开(公告)日：2003-07-08
申请号：US09879341
申请日：2001-06-11
申请人： Hubert Köster
发明人： Hubert Köster
IPC分类号： B01L900
CPC分类号： C12Q1/6872 , C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6883 , C12Q1/706 , G01N35/1067 , H01J49/00 , Y10T436/143333 , Y10T436/24 , C12Q2565/627 , C12Q2537/113 , C12Q2535/113 , C12Q2521/325 , C12Q2535/131 , C12Q2525/107 , C12Q2523/107 , C12Q2523/319 , C12Q2565/625 , C12Q2535/125
摘要： Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Arrays of oligonucleotides for performing mass spectrometric analyses are provided. In one aspect, a solid support is provided that includes a plurality of single-stranded oligonucleotides immobilized thereon by a linker cleavable under conditions of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry; and matrix for performing MALDI mass spectrometry, wherein the support comprises a flat surface.
摘要翻译：提供了用于检测分子中特定核酸分子和序列的基于质谱的快速和高精度的方法。提供了用于进行质谱分析的寡核苷酸阵列。在一个方面，提供了固体支持物，其包括通过在基质辅助激光解吸/电离（MALDI）质谱法的条件下可切割的接头固定在其上的多个单链寡核苷酸; 和用于进行MALDI质谱分析的基质，其中所述载体包括平坦表面。

6. 发明授权

US06500621B2 DNA diagnostics based on mass spectrometry 有权
公开(公告)号：US06500621B2
公开(公告)日：2002-12-31
申请号：US09796416
申请日：2001-02-28
申请人： Hubert Köster
发明人： Hubert Köster
IPC分类号： C12Q168
CPC分类号： C12Q1/6872 , C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6883 , C12Q1/706 , G01N35/1067 , H01J49/00 , Y10T436/143333 , Y10T436/24 , C12Q2565/627 , C12Q2537/113 , C12Q2535/113 , C12Q2521/325 , C12Q2535/131 , C12Q2525/107 , C12Q2523/107 , C12Q2523/319 , C12Q2565/625 , C12Q2535/125
摘要： Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Depending upon the sequence to be detected, the processes, for example, can be used to diagnose a genetic disease or a chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogen, or for determining identity or heredity.

7. 发明授权

US06485913B1 Systems and methods for performing reactions in an unsealed environment 有权
标题翻译：在非密封环境中进行反应的系统和方法
公开(公告)号：US06485913B1
公开(公告)日：2002-11-26
申请号：US09678620
申请日：2000-10-02
申请人： Thomas Becker , Hubert Köster , Charles Cantor
发明人： Thomas Becker , Hubert Köster , Charles Cantor
IPC分类号： C12Q168
CPC分类号： B01J19/16 , B01J2219/00317 , B01J2219/00367 , B01J2219/00378 , B01J2219/00495 , B01J2219/00603 , B01J2219/0068 , C40B60/14 , G01N2035/00287 , Y10T436/11 , Y10T436/25375
摘要： Open systems for performing submicroliter reactions are provided. The systems can include a support for performing the reaction; a liquid dispensing system for dispensing a submicroliter amount of a liquid to a site on or in the support; a temperature controlling device for regulating the temperature of the support; and an interface for controlling the amount of liquid dispensed from the liquid dispensing system are provided. Methods using the systems are also provided.
摘要翻译：提供了用于进行亚微米级反应的开放系统。该系统可以包括用于进行反应的支持物; 液体分配系统，用于将液体微量滴定到支撑体上或载体上的部位; 用于调节支撑件的温度的温度控制装置; 并且提供用于控制从液体分配系统分配的液体量的界面。还提供了使用该系统的方法。

8. 发明授权

US06387628B1 Mass spectrometric detection of polypeptides 有权
标题翻译：多肽的质谱检测
公开(公告)号：US06387628B1
公开(公告)日：2002-05-14
申请号：US09664977
申请日：2000-09-18
申请人： Daniel Little , Hubert Köster , G. Scott Higgins , David Lough
发明人： Daniel Little , Hubert Köster , G. Scott Higgins , David Lough
IPC分类号： C12Q160
CPC分类号： G01N33/6848 , G01N33/6818 , G01N33/6842 , G01N33/6851 , H01J49/00
摘要： A process for determining the identity of a target polypeptide using mass spectroscopy is provided. Depending on the target polypeptide to be identified, a process as disclosed can be used, for example, to diagnose a genetic disease or chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogenic organism; or for determining identity or heredity. Kits for performing the disclosed processes also are provided.
摘要翻译：提供了使用质谱法确定靶多肽的身份的方法。根据待鉴定的靶多肽，可以使用所公开的方法，例如诊断遗传疾病或染色体异常，疾病或病症的倾向或病原生物的感染; 或用于确定身份或遗传。还提供了用于执行所公开的处理的套件。

9. 发明授权

US06268131B1 Mass spectrometric methods for sequencing nucleic acids 失效
标题翻译：用于测序核酸的质谱法
公开(公告)号：US06268131B1
公开(公告)日：2001-07-31
申请号：US08990851
申请日：1997-12-15
申请人： Changwon Kang , Young-Soo Kwon , Young Tae Kim , Hubert Köster , Daniel P. Little , Maryanne J. Little , Guobing Xiang , David M. Lough , Charles Cantor
发明人： Changwon Kang , Young-Soo Kwon , Young Tae Kim , Hubert Köster , Daniel P. Little , Maryanne J. Little , Guobing Xiang , David M. Lough , Charles Cantor
IPC分类号： C12Q168
CPC分类号： C12Q1/6872 , B01J2219/00317 , B01J2219/00387 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00691 , B01J2219/00702 , B01J2219/00722 , C40B40/06 , C40B60/14 , C12Q2525/143
摘要： A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods use a modified Sanger sequencing strategy in which RNA polymerase is used to generate a set of nested RNA transcripts obtained by base-specific chain termination. These are analyzed by mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.
摘要翻译：提供了使用RNA聚合酶对核酸进行测序的质谱法，包括DNA依赖性和RNA依赖性RNA聚合酶。该方法使用修饰的Sanger测序策略，其中RNA聚合酶用于产生通过碱基特异性链终止获得的一组巢式RNA转录物。这些通过质谱分析。还提供了鉴定转录终止子序列或衰减子序列的方法。

10. 发明授权

US06221605B1 DNA diagnostics based on mass spectrometry 有权
公开(公告)号：US06221605B1
公开(公告)日：2001-04-24
申请号：US09504245
申请日：2000-02-15
申请人： Hubert Köster
发明人： Hubert Köster
IPC分类号： C12Q168
CPC分类号： C12Q1/6872 , C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6883 , C12Q1/706 , G01N35/1067 , H01J49/00 , Y10T436/143333 , Y10T436/24 , C12Q2565/627 , C12Q2537/113 , C12Q2535/113 , C12Q2521/325 , C12Q2535/131 , C12Q2525/107 , C12Q2523/107 , C12Q2523/319 , C12Q2565/625 , C12Q2535/125
摘要： Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and sequences in the molecules are provided. Depending upon the sequence to be detected, the processes, for example, can be used to diagnose a genetic disease or a chromosomal abnormality, a predisposition to a disease or condition, or infection by a pathogen, or for determining identity or heredity.

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