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1. 发明授权

US6096870A Sequential separation of whey 失效
标题翻译：乳清的顺序分离
公开(公告)号：US6096870A
公开(公告)日：2000-08-01
申请号：US76169
申请日：1998-05-04
申请人： Zahid Mozaffar , Salah H. Ahmed , Vinit Saxena , Quirinus Ronnie Miranda
发明人： Zahid Mozaffar , Salah H. Ahmed , Vinit Saxena , Quirinus Ronnie Miranda
IPC分类号： A21D2/26 , A23C9/146 , A23G9/52 , A23J1/20 , A23L1/068 , A23L1/305 , G01N30/38 , C07K16/04 , C07K14/47 , A23C9/12
CPC分类号： A21D2/263 , A23C9/1465 , A23G9/52 , A23J1/205 , A23L21/15 , A23L33/19 , A23V2002/00 , G01N2030/386 , Y02P20/582 , Y10S530/833
摘要： The present invention is related to the separation of whey proteins, particularly the sequential separation of whey proteins into separate fractions through the use of chromatography. The present invention further provides methods and compositions for the sequential separation of whey proteins, as well as their use in various products. The present invention also provides methods and compositions for the cleaning of chromatography resins used in the separation of whey proteins.
摘要翻译：本发明涉及乳清蛋白的分离，特别是通过使用色谱法将乳清蛋白顺序分离成单独的级分。本发明还提供了用于顺序分离乳清蛋白的方法和组合物，以及它们在各种产品中的用途。本发明还提供了清洗用于分离乳清蛋白质的色谱树脂的方法和组合物。

2. 发明授权

US5817354A High throughput debittering 失效
标题翻译：高吞吐量的脱脂
公开(公告)号：US5817354A
公开(公告)日：1998-10-06
申请号：US702760
申请日：1996-08-22
申请人： Zahid Mozaffar , Quirinus Ronnie Miranda , Vinit Saxena
发明人： Zahid Mozaffar , Quirinus Ronnie Miranda , Vinit Saxena
IPC分类号： A23L2/06 , A23L2/70 , A23L2/80 , A23L5/20 , C02F1/42
CPC分类号： A23L2/06 , A23L2/70 , A23L2/80 , A23L5/273
摘要： The present invention relates to methods and materials for use in processing citrus products, and more particularly, the debittering of juices. In particular, the present invention is suited to the debittering of citrus juices. In one preferred embodiment, the citrus juice debittered by the present invention is grapefruit or orange juice. The methods allow for high flow rates which, in turn offer advantages associated with permitting high throughput of juice to be treated.
摘要翻译：本发明涉及用于加工柑橘制品的方法和材料，更具体地说，涉及果汁的脱脂。特别地，本发明适用于柑橘汁的脱脂。在一个优选实施方案中，本发明所述的柑橘汁是葡萄柚或橙汁。这些方法允许高流速，这又提供与允许待处理的果汁高通量相关联的优点。

3. 发明授权

US6045842A High throughput debittering 失效
标题翻译：高吞吐量的脱脂
公开(公告)号：US6045842A
公开(公告)日：2000-04-04
申请号：US110492
申请日：1998-07-06
申请人： Zahid Mozaffar , Quirinus Ronnie Miranda , Vinit Saxena
发明人： Zahid Mozaffar , Quirinus Ronnie Miranda , Vinit Saxena
IPC分类号： A23L2/06 , A23L2/70 , A23L2/80 , A23L5/20 , C02F1/42
CPC分类号： A23L2/06 , A23L2/70 , A23L2/80 , A23L5/273
摘要： The present invention relates to methods and materials for use in processing citrus products, and more particularly, the debittering and/or deacidifying of juices. In one preferred embodiment, the citrus juice debittered and/or deacidified by the present invention is grapefruit, while in an alternative embodiment, the citrus juice debittered and/or deacidified is orange juice. The methods allow for high flow rates which, in turn offer advantages associated with permitting high throughput of juice to be treated.
摘要翻译：本发明涉及用于加工柑橘产品的方法和材料，更具体地说，涉及果汁的脱脂和/或脱酸。在一个优选的实施方案中，本发明所消耗的和/或脱酸的柑橘汁是葡萄柚，而在另一个实施方案中，柑橘汁被脱色和/或脱酸是橙汁。这些方法允许高流速，这又提供与允许待处理的果汁高通量相关联的优点。

4. 发明授权

US5756680A Sequential separation of whey proteins and formulations thereof 失效
标题翻译：乳清蛋白及其制剂的顺序分离
公开(公告)号：US5756680A
公开(公告)日：1998-05-26
申请号：US678364
申请日：1996-07-16
申请人： Salah H. Ahmed , Vinit Saxena , Zahid Mozaffar , Quirinus R. Miranda
发明人： Salah H. Ahmed , Vinit Saxena , Zahid Mozaffar , Quirinus R. Miranda
IPC分类号： A21D2/26 , A23C9/146 , A23J1/20 , A23L1/068 , A23L1/305 , C07K16/04 , G01N30/38 , A23C9/14 , C07K1/36 , C07K14/47
CPC分类号： C07K16/04 , A21D2/263 , A23C9/1465 , A23J1/205 , A23L21/15 , A23L33/19 , A23V2002/00 , G01N2030/386
摘要： A method is disclosed for the sequential separation of whey proteins using radial-flow chromatography. Different buffer systems adjusted to suitable pH and ionic strength are utilized in the separation process. The method separates at least five different proteins from whey. Infant feeding formulas, and other food formulations are also disclosed incorporating therein in different proportions various proteins separated from the whey.
摘要翻译：公开了使用径向流色谱法顺序分离乳清蛋白的方法。在分离过程中使用了调节到合适pH和离子强度的不同缓冲体系。该方法将至少五种不同的蛋白质与乳清分离。还公开了婴儿喂养配方和其它食品配方，其中以不同比例掺入了与乳清分离的各种蛋白质。

5. 发明授权

US4627918A Chromatography column using horizontal flow 失效
标题翻译：色谱柱使用水平流
公开(公告)号：US4627918A
公开(公告)日：1986-12-09
申请号：US794727
申请日：1985-11-04
申请人： Vinit Saxena
发明人： Vinit Saxena
IPC分类号： B01D15/08 , B01D15/14 , G01N30/38 , G01N30/52 , G01N30/60 , G01N30/92 , G01N30/94
CPC分类号： B01D15/14 , B01D15/1807 , G01N30/38 , B01D15/1885 , G01N2030/386 , G01N2030/522 , G01N30/6004 , G01N30/92 , G01N30/94
摘要： A method and apparatus for performing chromatographic separations utilizing horizontal flow through the separating medium bed are disclosed. The chromatographic separations may be carried out by using an apparatus involving a cylindrical column construction wherein the horizontal flow is in a radial direction as it passes through the separating medium bed or an apparatus involving a cubical column construction wherein the flow traverses from one end of the separating medium bed to the other end horizontally. The apparatus includes means for distributing the flow, achieving horizontal streamlines and collecting separated components. In one embodiment, the separating medium bed in adjustable in height.
摘要翻译：公开了一种利用水平流动通过分离介质床进行色谱分离的方法和装置。色谱分离可以通过使用包括圆柱形柱结构的装置来进行，其中水平流在其通过分离介质床时处于径向方向，或者涉及立方柱结构的装置，其中流动从水平分离介质床到另一端。该装置包括用于分配流动，实现水平流线和收集分离的部件的装置。在一个实施例中，高度可调的分离介质床。

6. 发明授权

US5462659A Chromatography column 失效
标题翻译：色谱柱
公开(公告)号：US5462659A
公开(公告)日：1995-10-31
申请号：US77427
申请日：1993-06-15
申请人： Vinit Saxena , Paul Young
发明人： Vinit Saxena , Paul Young
IPC分类号： B01D15/08 , G01N30/52 , G01N30/60
CPC分类号： B01D15/18 , B01D15/22 , B01D15/206 , B01D15/34 , G01N2030/522 , G01N30/6004
摘要： An improved axial flow chromatography column for separations dependent upon bed depth, such as size exclusion and where changes of column volume necessitate bed height adjustments. The improvements include a snap-action lever or a collet arrangement which permits quick and easy movement of the bed adjuster; a flush seal assembly at the outlet frit and a V-shaped seal in the bed adjuster which obviates dead volume gap of conventional O-ring seals; lens-shaped frits convex on the inlet and outlet sides provide for uniform flow through the column, and the access ports eliminate the contamination problem by providing easy access for cleaning and aspiration of liquid; and a combined inlet tube and adjuster tube assembly eliminates the need for threaded components which make sanitation difficult.
摘要翻译：用于根据床深度进行分离的改进的轴流色谱柱，例如尺寸排除和柱体积的变化需要床高度调节。这些改进包括快速操作杆或夹头装置，其允许床调节器的快速和容易的移动; 在出口玻璃料处的冲洗密封组件和床调节器中的V形密封件，其消除常规O形圈密封件的死体积间隙; 在入口侧和出口侧凸出的透镜状玻璃料提供均匀流过色谱柱的流通孔，通过提供易于清洗和吸取液体的方便消除污染问题; 并且组合的入口管和调节管组件消除了使得卫生困难的螺纹部件的需要。

7. 发明授权

US4740298A Chromatography column/moving belt interface 失效
标题翻译：色谱柱/移动带接口
公开(公告)号：US4740298A
公开(公告)日：1988-04-26
申请号：US904912
申请日：1986-09-08
申请人： Brian D. Andresen , Vinit Saxena
发明人： Brian D. Andresen , Vinit Saxena
IPC分类号： G01N30/38 , G01N30/72 , B01D15/08
CPC分类号： G01N30/7286 , G01N2030/386 , Y10T436/110833
摘要： A chromatography column/moving belt interface for directing the output of the column to a point of use, detection and/or identification, such as a mass spectrometer is disclosed. The chromatography column/moving belt interface of this invention thus provides for substantially simultaneous analysis of materials separated in the column. The chromatography column utilizes horizontal flow through the separating medium with the output therefrom being at or near atmospheric pressure. The moving belt interface includes means for directing the output of the column onto a moving belt at variable angles, means for vaporizing output which is deposited on the belt and means for providing a vacuum lock arrangement such that the belt moves from and through a near atmospheric pressure of the column output to a near vacuum condition of the mass spectrometer. The chromatography column may be of a type which has a quickly disposable or replaceable receptacle for the separating medium, such as a thin film member or wafer, or the medium itself or even the entire column may be constructed to be totally disposable or replaceable.
摘要翻译：公开了用于将柱的输出引导到使用点，检测和/或识别（例如质谱仪）的色谱柱/移动带接口。因此，本发明的层析柱/移动带接口提供了在柱中分离的材料的基本上同时的分析。色谱柱利用水平流过分离介质，其输出处于或接近大气压。移动带接口包括用于将柱的输出以可变角度引导到移动带上的装置，用于蒸发沉积在带上的输出的装置和用于提供真空锁定装置的装置，使得带移动并经过接近大气压柱输出的压力达到质谱仪的接近真空条件。色谱柱可以是具有用于分离介质的快速一次性或可替换的容器的类型，例如薄膜构件或晶片，或者介质本身，或者甚至整个柱可被构造为完全一次性的或可更换的。

8. 发明授权

US4676898A Chromatography column using horizontal flow 失效
标题翻译：色谱柱使用水平流
公开(公告)号：US4676898A
公开(公告)日：1987-06-30
申请号：US869295
申请日：1986-06-02
申请人： Vinit Saxena
发明人： Vinit Saxena
IPC分类号： B01D15/08 , B01D15/14 , G01N30/38 , G01N30/52 , G01N30/60 , G01N30/92 , G01N30/94
CPC分类号： G01N30/38 , B01D15/14 , B01D15/18 , B01D15/1885 , G01N2030/386 , G01N2030/522 , G01N30/6004 , G01N30/92 , G01N30/94
摘要： A chromatographic column is disclosed in which flow through the separating medium bed takes place horizontally. The column is packed with small, rigid, high performance separation medium. The fluid sample containing several components flows horizontally through the stationary phase or medium and is separated at least partially into discrete components. Means for providing even horizontal flow distribution of the sample fluid and for collection of the discrete components of the sample fluid are included. The stationary phase consists of a packed bed of high performance, small size particles of either (a) absorbants such as silica, alumina etc; (b) packing for reverse phase ion-exchange, affinity, hydrophobic, chiral or ion chromatography; (c) hydroxylapatite; or packing used for desalting. The column as a whole may be fabricated to be disposable or may be constructed to have a more simplified but durable housing.
摘要翻译：公开了一种色谱柱，其中通过分离介质床的流动水平地发生。该柱包装有小型，刚性，高性能的分离介质。含有几个组分的流体样品水平流过固定相或介质，并且至少部分地分离成离散组分。包括用于提供样品流体的均匀水平分布和用于收集样品流体的分立组分的装置。固定相由（a）吸收剂如二氧化硅，氧化铝等的高性能，小粒径的填充床组成; （b）用于反相离子交换，亲和力，疏水性，手性或离子色谱的包装; （c）羟基磷灰石; 或用于脱盐的包装。作为整体的塔可以被制造为一次性的或者可以构造成具有更简化但耐用的外壳。

9. 发明授权

US4840730A Chromatography system using horizontal flow columns 失效
标题翻译：色谱系统采用水平流柱
公开(公告)号：US4840730A
公开(公告)日：1989-06-20
申请号：US207896
申请日：1988-06-09
申请人： Vinit Saxena
发明人： Vinit Saxena
IPC分类号： B01D15/08 , G01N30/38 , G01N30/46 , G01N30/60 , G01N30/88
CPC分类号： G01N30/88 , B01D15/1807 , B01D15/1871 , B01D15/1885 , G01N2030/386 , G01N2030/8886 , G01N30/461 , G01N30/466 , G01N30/6047
摘要： A system for chromatography using horizontal (radial) flow columns is disclosed. The system provides rapid separation of complex biological or organic mixtures at low operating pressures. The system may be used in a variety of applications, such as in the analysis of the constituents of a mixture, or in large pilot or plant scale operations where the isolation of one or more purified components of a mixture or fluid is required for further testing or for commercial purposes. The system is designed to accommodate separation via a variety of routes including ion-exchange, affinity, hydrophobic, chiral, hydroxylapatite, reverse phased as well as rapid desalting techniques.
摘要翻译：公开了一种使用水平（径向）流动柱的色谱系统。该系统在低操作压力下快速分离复杂的生物或有机混合物。该系统可以用于各种应用中，例如在混合物的组分的分析中，或在大型试验或工厂规模操作中，其中需要分离混合物或流体的一种或多种纯化组分用于进一步测试或用于商业目的。该系统设计用于通过各种途径适应分离，包括离子交换，亲和力，疏水性，手性，羟基磷灰石，反向阶段以及快速脱盐技术。

10. 发明授权

US4833083A Packed bed bioreactor 失效
标题翻译：包装床生物反应器
公开(公告)号：US4833083A
公开(公告)日：1989-05-23
申请号：US54262
申请日：1987-05-26
申请人： Vinit Saxena
发明人： Vinit Saxena
IPC分类号： C12M1/40 , C12M3/06 , C12N5/02
CPC分类号： C12M25/18 , C12M41/26 , C12M41/32
摘要： A bioreactor for anchorage or nonanchorage dependent cells or for immobilized enzymes that is capable of achieving high cell densities or concentrations of reaction products is disclosed. The bioreactor is capable of being easily scaled up. The bioreactor utilizes horizontal or radial flow of the culture or growth medium across a packed bed of microcarriers with attached cells or enzymes. The radial flow utilized by the chamber minimizes the non-optimal culture perfusion problems and maximizes the uniformity of medium delivery and cell and/or enzyme viability. The cell culture chamber possess the capability for the production of many industrially important and useful products, particularly in the medical, pharmaceutical, food, agricultural, environmental and purification industries. In the medical and pharmaceutical industries, the large scale production of therapeutically important substances such as anti-tumor factors, hormones, viral antigens, enzymes, interferons and other valuable biomolecules, is greatly facilitated.
摘要翻译：公开了一种用于锚固或非锚定依赖性细胞或能够实现高细胞密度或反应产物浓度的固定化酶的生物反应器。生物反应器能够容易地放大。生物反应器利用培养物或生长培养基在具有附着细胞或酶的微载体的填充床上的水平或径向流动。腔室使用的径向流动使非最佳培养灌注问题最小化，并最大化培养基递送和细胞和/或酶活力的均匀性。细胞培养室具有生产许多工业上重要和有用的产品的能力，特别是在医药，制药，食品，农业，环境和净化行业。在医药行业，极大地促进了大量生产治疗重要物质如抗肿瘤因子，激素，病毒抗原，酶，干扰素和其他有价值的生物分子。

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