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1. 发明授权

US5756680A Sequential separation of whey proteins and formulations thereof 失效
标题翻译：乳清蛋白及其制剂的顺序分离
公开(公告)号：US5756680A
公开(公告)日：1998-05-26
申请号：US678364
申请日：1996-07-16
申请人： Salah H. Ahmed , Vinit Saxena , Zahid Mozaffar , Quirinus R. Miranda
发明人： Salah H. Ahmed , Vinit Saxena , Zahid Mozaffar , Quirinus R. Miranda
IPC分类号： A21D2/26 , A23C9/146 , A23J1/20 , A23L1/068 , A23L1/305 , C07K16/04 , G01N30/38 , A23C9/14 , C07K1/36 , C07K14/47
CPC分类号： C07K16/04 , A21D2/263 , A23C9/1465 , A23J1/205 , A23L21/15 , A23L33/19 , A23V2002/00 , G01N2030/386
摘要： A method is disclosed for the sequential separation of whey proteins using radial-flow chromatography. Different buffer systems adjusted to suitable pH and ionic strength are utilized in the separation process. The method separates at least five different proteins from whey. Infant feeding formulas, and other food formulations are also disclosed incorporating therein in different proportions various proteins separated from the whey.
摘要翻译：公开了使用径向流色谱法顺序分离乳清蛋白的方法。在分离过程中使用了调节到合适pH和离子强度的不同缓冲体系。该方法将至少五种不同的蛋白质与乳清分离。还公开了婴儿喂养配方和其它食品配方，其中以不同比例掺入了与乳清分离的各种蛋白质。

2. 发明授权

US5009997A Two site cross-reaction immunometric sandwich assay method 失效
标题翻译：两个位点交叉反应免疫夹心法
公开(公告)号：US5009997A
公开(公告)日：1991-04-23
申请号：US896353
申请日：1985-01-30
申请人： Vipin D. Shah , Padmakar v. Kulkarni , Quirinus R. Miranda
发明人： Vipin D. Shah , Padmakar v. Kulkarni , Quirinus R. Miranda
IPC分类号： G01N33/544 , G01N33/573
CPC分类号： G01N33/573 , G01N33/544 , Y10S435/805 , Y10S435/81 , Y10S435/975 , Y10S436/808
摘要： A two site cross-reaction immunometric sandwich assay method for the detection and measurement of an analyte, such as creatine phospho-kinase-MB, in serum comprising the selection of two different antibodies each of which is specific to a different analyte but each of which will cross-react with the analyte of interest. The first antibody is reacted with the unknown sample utilizing a solid-phase to bind the first antibody. Separation of the solid and liquid portions of the first reaction is accomplished and the solid portion thereof is reacted with the second antibody which is tagged. The solid portion and liquid portion of the second reaction are separated and the solid portion is tested for the tag as an indication of the presence of said analyte. With particular reference to testing for creatine phospho-kinase-MB in human serum, the cross-reacting antibodies utilized are antibody to creatine phospho-kinase-BB and creatine phospho-kinase-MM.
摘要翻译：用于在血清中检测和测量分析物如肌酸磷酸激酶-MB的两个位点交叉反应免疫三明治测定方法，包括选择两种不同的抗体，每种抗体对不同的分析物是特异性的，但是每种将与感兴趣的分析物交叉反应。使用固相将第一抗体与未知样品反应以结合第一抗体。完成第一反应的固体和液体部分的分离，其固体部分与标记的第二抗体反应。分离第二反应的固体部分和液体部分，并测试固体部分作为所述分析物的存在的指示。特别参考人血清中肌酸磷酸激酶-MB的检测，所用的交叉反应抗体是针对肌酸磷酸激酶-BB和肌酸磷酸激酶-MM的抗体。

3. 发明授权

US5009996A Two site cross-reaction immunometric sandwich assay method 失效
标题翻译：两个位点交叉反应免疫夹心法
公开(公告)号：US5009996A
公开(公告)日：1991-04-23
申请号：US537947
申请日：1983-09-30
申请人： Vipin D. Shah , Padmaker V. Kulkarni , Quirinus R. Miranda
发明人： Vipin D. Shah , Padmaker V. Kulkarni , Quirinus R. Miranda
IPC分类号： G01N33/544 , G01N33/573
CPC分类号： G01N33/544 , G01N33/573 , Y10S435/805 , Y10S435/81
摘要： A two site cross-reaction immunometric sandwich assay method for the detection and measurement of an analyte, such as creatine phospho-kinase-MB, in serum comprising the selection of two different antibodies each of which is specific to a different analyte but each of which will cross-react with the analyte of interest. The first antibody is reacted with the unknown sample utilizing a solid-phase to bind the first antibody. Separation of the solid and liquid portions of the first reaction is accomplished and the solid portion thereof is reacted with the second antibody which is tagged. The solid portion and liquid portion of the second reaction are separated and the solid portion is tested for the tag as an indication of the presence of said analyte. With particular reference to testiong for creatine phospho-kinase-MB in human serum, the cross-reacting antibodies utilized are antibody to creatine phospho-kinase-BB and creatine phospho-kinase-MM.
摘要翻译：用于在血清中检测和测量分析物如肌酸磷酸激酶-MB的两个位点交叉反应免疫三明治测定方法，包括选择两种不同的抗体，每种抗体对不同的分析物是特异性的，但是每种将与感兴趣的分析物交叉反应。使用固相将第一抗体与未知样品反应以结合第一抗体。完成第一反应的固体和液体部分的分离，其固体部分与标记的第二抗体反应。分离第二反应的固体部分和液体部分，并测试固体部分作为所述分析物的存在的指示。特别参考人血清中肌酸磷酸激酶-MB的检测，所用的交叉反应抗体是肌酸磷酸激酶-BB和肌酸磷酸激酶-MN的抗体。

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