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1. 发明授权

US11221317B2 Development chambers for thin layer chromatography and methods of making and using the same 有权
公开(公告)号：US11221317B2
公开(公告)日：2022-01-11
申请号：US16662534
申请日：2019-10-24
申请人： Sorbent Technologies, Inc.
发明人： Robert Ralph Kerr
IPC分类号： G01N30/92 , G01N30/94
摘要： Development chambers suitable for use in thin layer chromatography are disclosed. Methods of making and using development chambers in thin layer chromatography are also disclosed.

2. 发明授权

US09726649B2 Chromatographic medium 有权
公开(公告)号：US09726649B2
公开(公告)日：2017-08-08
申请号：US14363515
申请日：2013-01-09
申请人： DAICEL CORPORATION
发明人： Toshiharu Minoda , Isamu Ikeda
IPC分类号： B01D15/08 , B01J20/285 , G01N30/93 , G01N30/92 , B01J20/291 , B01J20/32 , G01N30/94 , G01N30/88
CPC分类号： G01N30/92 , B01D15/08 , B01J20/291 , B01J2220/80 , G01N30/93 , G01N2030/8877
摘要： A chromatographic medium having a separating agent layer, which is used to separate target substances, and a permeation layer, which is laminated so as to face the separating agent layer and which is used to enable permeation of the target substances separated by the separating agent layer, wherein a region in which the permeation layer is not laminated is present on a part of the separating agent layer, the separating agent layer exhibits a separating property for the target substances and exhibits an optical responsiveness to ultraviolet rays, and the permeation layer exhibits an optical responsiveness that is different from those of the target substances and the separating agent layer.

3. 发明授权

US09234881B2 Sample detection method by thin-layer chromatography, thin-layer chromatography plate, and method for producing same 有权
标题翻译：薄层色谱法，薄层色谱板样品检测方法及其制备方法
公开(公告)号：US09234881B2
公开(公告)日：2016-01-12
申请号：US13700223
申请日：2011-05-27
申请人： Toshiharu Minoda , Isamu Ikeda , Satoru Nose , Noriaki Takeya , Ichiro Okamoto
发明人： Toshiharu Minoda , Isamu Ikeda , Satoru Nose , Noriaki Takeya , Ichiro Okamoto
IPC分类号： G01N30/90 , G01N30/94 , G01N30/92
CPC分类号： G01N30/90 , B01D15/20 , B01D15/265 , B01J20/045 , B01J20/26 , B01J20/3042 , G01N30/92 , G01N30/93 , G01N30/94 , G01N2030/945
摘要： A thin-layer chromatography plate and a thin-layer chromatography uses a thin-layer chromatography plate that has a first separating agent layer, which has the same optical response as the sample, and a second separating agent layer, which is disposed adjacent thereto and has an optical response that is different from the aforementioned optical response; carries out development of the sample in the first separating agent layer; thereafter carries out further development of the spots from the first separating agent layer toward the second separating agent layer; and detects the spots in the second separating agent layer through exposure to ultraviolet radiation or a color-development treatment with a coloring agent.
摘要翻译：薄层色谱板和薄层色谱法使用具有与样品相同的光学响应的第一分离剂层的薄层色谱板和与其相邻设置的第二分离剂层，具有与上述光学响应不同的光学响应; 在第一分离剂层中进行样品的开发; 然后进一步开发从第一分离剂层朝向第二分离剂层的斑点; 并通过暴露于紫外线照射或着色剂的显色处理来检测第二分离剂层中的斑点。

4. 发明申请

US20090081796A1 METHOD FOR THE DETERMINATION OF PHOSPHOLIPIDS IN AMNIOTIC FLUID SAMPLES 审中-公开
标题翻译：用于确定生物流体样品中磷脂的方法
公开(公告)号：US20090081796A1
公开(公告)日：2009-03-26
申请号：US12159768
申请日：2007-07-31
申请人： Maria Irene De Oliveira Monteiro Jesus Rebelo , Belmiro Dos Santos Patricio
发明人： Maria Irene De Oliveira Monteiro Jesus Rebelo , Belmiro Dos Santos Patricio
IPC分类号： G01N30/94
CPC分类号： G01N30/94 , G01N2030/945
摘要： The present invention refers to a fast method for the determination of phospholipids in amniotic fluid samples by HPTLC (high performance thin layer chromatography). This innovative method presents high sensitivity, reproducibility and resolution. It has also the advantage of being of easy laboratorial interpretation, allowing the elimination of false results due to the contamination of the sample with blood or meconium.
摘要翻译：本发明涉及通过HPTLC（高效薄层色谱法）测定羊水样品中磷脂的快速方法。这种创新的方法具有高灵敏度，重复性和分辨率。它还具有易于实验解释的优点，允许消除由于样品与血液或胎粪的污染导致的错误结果。

5. 发明授权

US4676898A Chromatography column using horizontal flow 失效
标题翻译：色谱柱使用水平流
公开(公告)号：US4676898A
公开(公告)日：1987-06-30
申请号：US869295
申请日：1986-06-02
申请人： Vinit Saxena
发明人： Vinit Saxena
IPC分类号： B01D15/08 , B01D15/14 , G01N30/38 , G01N30/52 , G01N30/60 , G01N30/92 , G01N30/94
CPC分类号： G01N30/38 , B01D15/14 , B01D15/18 , B01D15/1885 , G01N2030/386 , G01N2030/522 , G01N30/6004 , G01N30/92 , G01N30/94
摘要： A chromatographic column is disclosed in which flow through the separating medium bed takes place horizontally. The column is packed with small, rigid, high performance separation medium. The fluid sample containing several components flows horizontally through the stationary phase or medium and is separated at least partially into discrete components. Means for providing even horizontal flow distribution of the sample fluid and for collection of the discrete components of the sample fluid are included. The stationary phase consists of a packed bed of high performance, small size particles of either (a) absorbants such as silica, alumina etc; (b) packing for reverse phase ion-exchange, affinity, hydrophobic, chiral or ion chromatography; (c) hydroxylapatite; or packing used for desalting. The column as a whole may be fabricated to be disposable or may be constructed to have a more simplified but durable housing.
摘要翻译：公开了一种色谱柱，其中通过分离介质床的流动水平地发生。该柱包装有小型，刚性，高性能的分离介质。含有几个组分的流体样品水平流过固定相或介质，并且至少部分地分离成离散组分。包括用于提供样品流体的均匀水平分布和用于收集样品流体的分立组分的装置。固定相由（a）吸收剂如二氧化硅，氧化铝等的高性能，小粒径的填充床组成; （b）用于反相离子交换，亲和力，疏水性，手性或离子色谱的包装; （c）羟基磷灰石; 或用于脱盐的包装。作为整体的塔可以被制造为一次性的或者可以构造成具有更简化但耐用的外壳。

6. 发明授权

US4292041A Surfactant assay 失效
标题翻译：表面活性剂测定
公开(公告)号：US4292041A
公开(公告)日：1981-09-29
申请号：US90768
申请日：1979-11-02
申请人： W. Wardle Fullerton
发明人： W. Wardle Fullerton
IPC分类号： G01N30/94 , G01N31/08
CPC分类号： G01N30/94 , Y10T436/143333
摘要： Thin layer chromatography is used to separate various surfactants. The support medium is silica gel on a glass or acrylamide plate. The developing solvent is chloroform/methanol/water. After drying, the surfactants are detected at extremely low levels with iodine vapor, rhodamine 6G in methanol or phosphomolybdic acid in ethanol/ether, or radiolabelled surfactant. A method is given for the separation of surfactants from proteins or glycoproteins or lipoproteins or polysaccharides, or lipopolysaccharides.
摘要翻译：薄层色谱用于分离各种表面活性剂。载体介质是玻璃或丙烯酰胺板上的硅胶。显影溶剂为氯仿/甲醇/水。干燥后，用碘蒸气，在甲醇中的罗丹明6G或乙醇/乙醚中的磷钼酸或放射性标记的表面活性剂检测表面活性剂。给出了从蛋白质或糖蛋白或脂蛋白或多糖或脂多糖分离表面活性剂的方法。

7. 发明授权

US4273653A Microchromatographic system for medicament intake control 失效
标题翻译：用于药物摄入控制的微量色谱系统
公开(公告)号：US4273653A
公开(公告)日：1981-06-16
申请号：US122732
申请日：1980-02-19
申请人： Michael Uihlein
发明人： Michael Uihlein
IPC分类号： G01N30/94 , G01N33/52
CPC分类号： G01N30/94 , G01N33/521
摘要： Microchromatographic system which consists of a test tube serving as developing chamber (2) and being closed by a stopper (3), and a thin-layer plate or foil in the form of a strip (1) fitting into the test tube, said plate or foil being coated in its lower quarter with a layer for concentration and purification (a) which merges without interruption into a thin-layer chromatographic separating layer (b) covering the remainder of the strip.
摘要翻译：由用作显影室（2）并由止动器（3）封闭的试管构成的微量色谱系统，以及装配到试管中的条带（1）形式的薄层板或箔，所述板或箔在其下部四分之一中涂覆有用于浓缩和纯化的层（a），其不间断地合并到覆盖条的其余部分的薄层色谱分离层（b）中。

8. 发明授权

US4125464A Method and apparatus for multiple development of thin-layer chromatography plates 失效
标题翻译：薄层色谱板多发展的方法和装置
公开(公告)号：US4125464A
公开(公告)日：1978-11-14
申请号：US841237
申请日：1977-10-11
申请人： Klaus Burger , Hans Mausberg , Hubert Tengler
发明人： Klaus Burger , Hans Mausberg , Hubert Tengler
IPC分类号： G01N30/95 , G01N30/94 , B01D15/08
CPC分类号： G01N30/94
摘要： Method and apparatus for the automatic multiple development of thin-layer chromatography plates. Instead of drying the plates by irradiation a laminar stream of inert gas is passed over the plate. Substances, which decompose at higher temperature, may be treated by chromatography, whereby reproducibility and sensitivity are higher and the productivity is increased.
摘要翻译：薄层色谱板自动多次开发的方法和装置。代替通过照射干燥板，使惰性气体层流通过该板。在较高温度下分解的物质可以通过色谱法处理，由此再现性和灵敏度更高，生产率提高。

9. 发明授权

US3904372A Automatic thin layer chromatographic apparatus 失效
标题翻译：自动薄层色谱仪
公开(公告)号：US3904372A
公开(公告)日：1975-09-09
申请号：US32286973
申请日：1973-01-11
申请人： LIGHTNER GENE E
发明人： LIGHTNER GENE E
IPC分类号： G01N30/90 , G01N30/94 , G01N31/08 , B01D15/08
CPC分类号： G01N30/90 , G01N30/94
摘要： The automatic apparatus described utilizes two pivotable arms, one a spotter arm, the second a pick-up arm. The pick-up arm operates to pick-up chromatographic plates from a supply magazine and place them in a position for spotting. After being spotted, with an aliquot of a sample fluid to be analyzed, the plates are transferred by the pick-up arm into discrete vertically disposed tanks positioned about a turntable. Solvent is applied to each tank immediately prior to insertion of the spotted plate therein. Upon development of a plate, usually one complete rotation of the tank turntable and after the solvent is drained from the tank, the pick-up arm picks up the developed plate and passes it down a chute to an automatic reader. The spotter arm has a capillary tube mounted on its end portion. This single tube is dipped into each sample vial which is disposed about a sample turntable in a ring-like fashion and transfers a sample aliquot to a precise position on the lower edge of a chromatographic plate. A reference sample aliquot is placed directly over the spot to provide an internal standard.
摘要翻译：所描述的自动装置使用两个可枢转的臂，一个是分拣臂，第二个拾取臂。拾取臂用于从供应盒拾取色谱板，并将其放置在用于检测的位置。在被点样后，用待分析的样品流体的等分试样，将板通过拾取臂转移到位于转盘周围的离散垂直设置的槽中。在将斑点板插入其中之前立即将溶剂施加到每个罐。在板的开发中，通常是罐转盘的一个完整的旋转，并且在溶剂从罐中排出之后，拾取臂拾取显影的板并将其沿着滑槽传送到自动读取器。扫瞄臂具有安装在其端部上的毛细管。将该单个管浸入每个样品瓶中，每个样品瓶围绕样品转盘以环状方式设置，并将样品等分试样转移到色谱板下边缘上的精确位置。参考样品等分试样直接放在现场以提供内标。

10. 发明授权

US3547023A Chromatographic chamber 失效
公开(公告)号：US3547023A
公开(公告)日：1970-12-15
申请号：US3547023D
申请日：1968-06-28
申请人： EASTMAN KODAK CO
发明人： LESTIENNE ANDRE F P , BOUDIN GEORGES M
IPC分类号： G01N30/90 , G01N30/94 , G03D3/00
CPC分类号： G01N30/94 , G01N30/90

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