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1. 发明授权

US06465190B1 Method for producing DNA chip 失效
标题翻译：生产DNA芯片的方法
公开(公告)号：US06465190B1
公开(公告)日：2002-10-15
申请号：US09694135
申请日：2000-10-23
申请人： Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi , Takao Ohnishi
发明人： Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi , Takao Ohnishi
IPC分类号： C12Q168
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00619 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/10 , B01L2400/0442 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要： Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要翻译：公开了一种方法，其包括在基板的表面上形成聚-L-赖氨酸层的预处理步骤，制备含有DNA片段的样品的样品制备步骤，稀释所得样品的浓度的稀释步骤，以及将稀释的样品溶液供应到基板上以产生DNA芯片的供给步骤。样品制备步骤包括PCR扩增DNA片段以制备PCR产物的扩增步骤，将获得的PCR产物干燥以形成DNA粉末的粉末形成步骤和将获得的DNA粉末溶解在缓冲溶液中的混合步骤。

2. 发明授权

US06852545B2 Micropipette and dividedly injectable apparatus 有权
标题翻译：微量拨液器和分注式装置
公开(公告)号：US06852545B2
公开(公告)日：2005-02-08
申请号：US10453283
申请日：2003-06-03
申请人： Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi
发明人： Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi
IPC分类号： B01J19/00 , B01L3/00 , B01L3/02 , B01L7/00 , B01L9/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N35/00 , G01N35/10 , G01N1/10
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0241 , B01L3/0268 , B01L3/5085 , B01L3/5088 , B01L9/54 , B01L2200/021 , B01L2200/027 , B01L2300/0816 , B01L2300/0819 , B01L2400/027 , B01L2400/0439 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , Y10T436/25 , Y10T436/2575 , C12Q2565/507
摘要： A micropipette is provided including at least one substrate, an inlet port through which a sample is delivered from the outside, a cavity to be poured and filled with the sample, an introduction hole, and an injection port for expelling the sample are formed on the at least one substrate. The substrate for forming the cavity is made of ceramics, a piezoelectric/electrostrictive element is provided for at least one wall surface of the substrate, and the sample moves as a laminar flow in the cavity. The volume of the cavity is changed by driving the piezoelectric/electrostrictive element to expell a certain amount of the sample in the cavity from the injection port. According to the micropipette, it is possible to form microspots with high accuracy and high speed. According to a dispenser including the micropipette, it is possible to efficiently dispense hundreds to ten thousands of different samples at one time and form microspots. Therefore, productivity is remarkably improved.
摘要翻译：提供了一种微量移液管，其包括：至少一个基板，从外部输送样品的入口，待注入的空腔并填充样品，引入孔和用于排出样品的注射口至少一个基底。用于形成腔的衬底由陶瓷制成，为衬底的至少一个壁表面提供压电/电致伸缩元件，并且样品在空腔中以层流的形式移动。通过驱动压电/电致伸缩元件来改变空腔的体积，以从注入口排出一定量的空腔中的样品。根据微量移液管，可以以高精度和高速度形成微孢子。根据包括微量移液管的分配器，可以一次有效地分配数百到数千个不同的样品并形成微点。因此，生产力显着提高。

3. 发明授权

US06656432B1 Micropipette and dividedly injectable apparatus 失效
标题翻译：微量拨液器和分注式装置
公开(公告)号：US06656432B1
公开(公告)日：2003-12-02
申请号：US09429140
申请日：1999-10-28
申请人： Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi
发明人： Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi
IPC分类号： G01N302
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0241 , B01L3/0268 , B01L3/5085 , B01L3/5088 , B01L9/54 , B01L2200/021 , B01L2200/027 , B01L2300/0816 , B01L2300/0819 , B01L2400/027 , B01L2400/0439 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , Y10T436/25 , Y10T436/2575 , C12Q2565/507
摘要： A micropipette is provided including at least one substrate, an inlet port through which a sample is delivered from the outside, a cavity to be poured and filled with the sample, an introduction hole, and an injection port for expelling the sample are formed on the at least one substrate. The substrate for forming the cavity is made of ceramics, a piezoelectric/electrostrictive element is provided for at least one wall surface of the substrate, and the sample moves as a laminar flow in the cavity. The volume of the cavity is changed by driving the piezoelectric/electrostrictive element to expell al certain amount of the sample in the cavity from the injection port. According to the micropipette, it is possible to form microspots with high accuracy and high speed. According to a dispenser including the micropipette, it is possible to efficiently dispense hundreds to ten thousands of different samples at one time and form microspots. Therefore, productivity is remarkably improved.
摘要翻译：提供了一种微量移液管，其包括至少一个基板，从外部输送样品的入口，待注入的空腔并填充有样品，引入孔和用于排出样品的注射口形成在至少一个基底。用于形成腔的衬底由陶瓷制成，为衬底的至少一个壁表面提供压电/电致伸缩元件，并且样品在空腔中以层流的形式移动。通过驱动压电/电致伸缩元件来改变空腔的体积，以从注入口排出空腔中的一定量的样品。根据微量移液管，可以以高精度和高速度形成微孢子。根据包括微量移液管的分配器，可以一次有效地分配数百到数千个不同的样品并形成微点。因此，生产力显着提高。

4. 发明授权

US06776960B2 Method for producing DNA chip 失效
标题翻译：生产DNA芯片的方法
公开(公告)号：US06776960B2
公开(公告)日：2004-08-17
申请号：US10128777
申请日：2002-04-24
申请人： Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi , Takao Ohnishi
发明人： Toshikazu Hirota , Nobuo Takahashi , Yukihisa Takeuchi , Takao Ohnishi
IPC分类号： G01N1506
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00619 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/10 , B01L2400/0442 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/1039 , C12Q2565/507
摘要： Disclosed is a method comprising a pretreatment step of forming a poly-L-lysine layer on a surface of a base plate, a sample preparation step of preparing a sample containing a DNA fragment, a dilution step of diluting the concentration of the obtained sample, and a supply step of supplying a diluted sample solution onto the base plate to produce a DNA chip. The sample preparation step includes an amplification step of PCR-amplifying the DNA fragment to prepare a PCR product, a powder formation step of drying the obtained PCR product to form DNA powder, and a mixing step of dissolving the obtained DNA powder in a buffer solution.
摘要翻译：公开了一种方法，其包括在基板的表面上形成聚-L-赖氨酸层的预处理步骤，制备含有DNA片段的样品的样品制备步骤，稀释所得样品的浓度的稀释步骤，以及将稀释的样品溶液供应到基板上以产生DNA芯片的供给步骤。样品制备步骤包括PCR扩增DNA片段以制备PCR产物的扩增步骤，将获得的PCR产物干燥以形成DNA粉末的粉末形成步骤和将获得的DNA粉末溶解在缓冲溶液中的混合步骤。

5. 发明授权

US5260717A Recording head having electrically insulating layer having optimum surface waviness and roughness 失效
标题翻译：具有电绝缘层的记录头具有最佳表面波纹度和粗糙度
公开(公告)号：US5260717A
公开(公告)日：1993-11-09
申请号：US933189
申请日：1992-08-20
申请人： Yukihisa Takeuchi , Toshikazu Hirota , Nobuo Takahashi
发明人： Yukihisa Takeuchi , Toshikazu Hirota , Nobuo Takahashi
IPC分类号： B41J2/325 , B41J2/32 , B41J2/335
CPC分类号： B41J2/3351 , B41J2/3353 , B41J2/33545 , B41J2/3355 , B41J2/33565
摘要： A recording head including an electrically insulating layer, a plurality of recording electrodes and at least one return circuit electrode provided on the opposite sides of the insulating layer, the insulating layer and electrodes being adapted to be held, at a distal end of the head, in contact with the resistive layer. The electrically insulating layer has a surface waviness represented by maximum filtered waviness, of not larger than 0.40 .mu.m, where a high band cut-off value is 0.8 mm and a reference length is 2.5 mm, as measured in main scanning direction perpendicular to a stacking direction of the electrically insulating layer, the recording and return circuit electrodes. The insulating layer also has a surface roughness represented by center-line mean roughness, within a range of 0.02 .mu.m to 0.4 .mu.m, where a cut-off value is 0.8 mm and a measuring length is 2.5 mm, as measured in the main scanning direction.
摘要翻译：一种记录头，包括电绝缘层，多个记录电极和设置在绝缘层的相对侧上的至少一个返回电路电极，绝缘层和电极适于保持在头部的远端，与电阻层接触。电绝缘层具有由最大滤波波纹度表示的不大于0.40μm的表面波纹，其中高频带截止值为0.8mm，参考长度为2.5mm，如在垂直于电绝缘层的堆叠方向，记录和返回电路电极。绝缘层还具有由中心线平均粗糙度表示的表面粗糙度，在0.02μm至0.4μm的范围内，其中截止值为0.8mm，测量长度为2.5mm，如主要测量扫描方向。

6. 发明授权

US5101221A Recording head distal-end substrate having opposed recording electrode array and return circuit electrode sheet 失效
标题翻译：具有对准记录电极阵列和返回电路电极片的记录头端基底
公开(公告)号：US5101221A
公开(公告)日：1992-03-31
申请号：US694489
申请日：1991-05-02
申请人： Yukihisa Takeuchi , Toshikazu Hirota , Shigeki Okada
发明人： Yukihisa Takeuchi , Toshikazu Hirota , Shigeki Okada
IPC分类号： B41J2/325 , B41J2/32 , B41J2/335 , B41J2/395
CPC分类号： B41J2/3351 , B41J2/33545 , B41J2/33565 , B41J2/395
摘要： A recording head operable to apply an electric current to an electrically resistive layer provided on a recording medium or a planar intermediate member interposed between the medium and the recording head. The head includes an electrically insulating substrate, and at least one recording electrode formed on one of opposite major surfaces of the substrate. The substrate is formed of an electrically insulating material whose wear resistance is lower than that of the recording electrode or electrodes. The substrate has a distal end portion extending from a proximal portion by a predetermined distance from the proximal portion for contact with the electrically resistive layer. The distal end portion has a thickness smaller than that of the proximal portion, as measured in a direction perpendicular to a direction of extension of the distal end portion. At least one return circuit electrode formed from a metal or metal alloy sheet is provided on the other major surface of the substrate remote from the recording electrode(s).

7. 发明授权

US06925693B2 Method of fabricating a piezoelectric/electrostrictive device 失效
标题翻译：制造压电/电致伸缩器件的方法
公开(公告)号：US06925693B2
公开(公告)日：2005-08-09
申请号：US09963696
申请日：2001-09-26
申请人： Yukihisa Takeuchi , Tsutomu Nanataki , Toshikazu Hirota , Koji Kimura
发明人： Yukihisa Takeuchi , Tsutomu Nanataki , Toshikazu Hirota , Koji Kimura
IPC分类号： H01L41/09 , H01L41/24 , H04R17/00
CPC分类号： H01L41/0946 , H01L41/314 , Y10T29/42 , Y10T29/49002 , Y10T29/4902 , Y10T29/49078 , Y10T29/49126 , Y10T29/49798 , Y10T83/0476 , Y10T83/0505 , Y10T83/051 , Y10T83/0577
摘要： A method of fabricating a piezoelectric/electrostrictive device including a driving portion, a movable portion, and a fixing portion for holding the driving portion and the movable portion. The driving portion includes a pair of thin plate portions facing each other, and a film-like piezoelectric/electrostrictive element formed on at least a part of the outer surface of at least one of the thin plate portions. A ceramic green laminate including at least one ceramic green sheet constituting one of the thin plates and at least one ceramic green sheet having at least one hole formed thereon is formed. The ceramic green laminate is sintered to produce a ceramic laminate. A piezoelectric/electrostrictive element is formed by a film formation method on the outer surface of the thin plate portion of the ceramic laminate. The ceramic laminate is then cut with the piezoelectric/electrostrictive element formed thereon.
摘要翻译：一种制造压电/电致伸缩器件的方法，包括驱动部分，可移动部分和用于保持驱动部分和可动部分的固定部分。驱动部分包括彼此面对的一对薄板部分，以及形成在至少一个薄板部分的外表面的至少一部分上的薄膜状压电/电致伸缩元件。形成包括构成薄板之一的至少一个陶瓷生片和至少形成有至少一个孔的至少一个陶瓷生片的陶瓷生坯层压体。将陶瓷生坯层压体烧结以制造陶瓷层压板。在陶瓷层叠体的薄板部分的外表面上通过成膜方法形成压电/电致伸缩元件。然后用形成在其上的压电/电致伸缩元件切割陶瓷层压体。

8. 发明授权

US06751832B2 Piezoelectric/electrostrictive device and production method thereof 失效
标题翻译：一种制造压电/电致伸缩器件的方法
公开(公告)号：US06751832B2
公开(公告)日：2004-06-22
申请号：US09961836
申请日：2001-09-24
申请人： Toshikazu Hirota , Koji Kimura , Yukihisa Takeuchi
发明人： Toshikazu Hirota , Koji Kimura , Yukihisa Takeuchi
IPC分类号： H04R1700
CPC分类号： H01L41/314 , G02B26/04 , G02B26/0858 , H01L41/0946 , Y10T29/42 , Y10T29/43 , Y10T29/49005 , Y10T29/49126 , Y10T29/49798
摘要： A method of manufacturing a piezoelectric/electrostrictive device includes the following steps. An integrated layered body is obtained by laminating at least one green sheet that is to be a thin plate, at least one green sheet having at least one rectangular-shaped hole portion, and at least one green sheet that is to be another thin plate. A piezoelectric/electrostrictive element is formed on a surface of the green sheets that form the thin plates by one of a thick film method or a thin film method. The layered body is cut in the laminating direction of the green sheets such that the rectangular-shaped hole portion is open on the side of the layered body after sintering the layered body.
摘要翻译：一种制造压电/电致伸缩器件的方法包括以下步骤。通过层叠至少一个作为薄板的生片，至少一个具有至少一个矩形孔部分的生片和至少一个作为另一薄板的生片来获得整体层叠体。压电/电致伸缩元件通过厚膜法或薄膜法之一形成在生成薄板的生片的表面上。在生片的层叠方向上切割层叠体，使得在烧结层叠体之后，矩形孔部在层叠体侧开口。

9. 发明授权

US06649343B1 DNA chip and method for producing the same 失效
标题翻译： DNA芯片及其制造方法
公开(公告)号：US06649343B1
公开(公告)日：2003-11-18
申请号：US09693228
申请日：2000-10-20
申请人： Toshikazu Hirota , Takao Ohnishi , Yukihisa Takeuchi
发明人： Toshikazu Hirota , Takao Ohnishi , Yukihisa Takeuchi
IPC分类号： C12Q168
CPC分类号： G01N35/00029 , B01J19/0046 , B01J2219/00317 , B01J2219/00351 , B01J2219/00369 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , B01L3/0268 , B01L3/5088 , B01L7/52 , B01L2300/0819 , B01L2400/0415 , B01L2400/0439 , C12Q1/6837 , C40B40/06 , C40B60/14 , G01N2035/00158 , G01N2035/00237 , G01N2035/1039 , C12Q2565/507
摘要： A DNA chip includes a large number of minute spots formed by dripping sample solutions onto a base plate. The base plate is provided with positional deviation-correcting means for displacing the spots and automatically correcting any positional deviation of each of the spots. The positional deviation-correcting means includes any one of at least one projection, at least one recess and electric field-generating means for providing charged states on the base plate centered at a correct position for each of the spots supplied on the base plate.
摘要翻译： DNA芯片包括通过将样品溶液滴落到基板上而形成的大量微小斑点。基板设置有用于移动斑点并自动校正每个斑点的任何位置偏差的位置偏差校正装置。位置偏差校正装置包括至少一个突起，至少一个凹部和电场产生装置中的任何一个，用于在基板上以提供在基板上的每个点的正确位置为中心提供充电状态。

10. 发明授权

US5909234A End-contact type thermal recording head having heat-generating portion on thin-walled end portion of ceramic substrate 失效
标题翻译：在陶瓷基板的薄壁端部具有发热部的端接型热敏记录头
公开(公告)号：US5909234A
公开(公告)日：1999-06-01
申请号：US829812
申请日：1997-04-21
申请人： Yukihisa Takeuchi , Toshikazu Hirota
发明人： Yukihisa Takeuchi , Toshikazu Hirota
IPC分类号： B41J2/335
CPC分类号： B41J2/33535 , B41J2/33545 , B41J2/3355 , B41J2/33565 , B41J2/3358
摘要： A thermal recording head having an electrically resistive heat-generating portion formed on a thin-walled end portion of a ceramic substrate and electrically connected to recording and return-circuit electrodes. A reinforcing or heat radiating member is disposed on at least one of opposite sides of the substrate such that a portion of the reinforcing or heat radiating member is located at the thin-walled end portion of the substrate. The thin-walled end portion is preferably partially defined by a shoulder surface which extends from one of opposite major surface of the substrate and terminates in the end face of the thin-walled end portion. The substrate preferably has a thermal conductivity within a range between 0.002 cal.multidot.cm/sec.multidot.cm.sup.2 .multidot..degree.C. and 0.03 cal.multidot.cm/sec.multidot.cm.sup.2 .multidot..degree.C., while the heat radiating member preferably has a thermal conductivity higher than 0.01 cal.multidot.cm/sec.multidot.cm.sup.2 .multidot..degree.C. The conductivity of the substrate is preferably lower than that of the heat radiating member.
摘要翻译：一种热敏记录头，其具有形成在陶瓷基板的薄壁端部上并电连接到记录和回路电极的电阻发热部分。加强或散热构件设置在基板的相对侧中的至少一个上，使得加强或散热构件的一部分位于基板的薄壁端部。优选地，所述薄壁端部部分地由从所述基板的相对主表面之一延伸并且终止于所述薄壁端部的端面的台肩表面限定。基板的导热率优选为0.002ccxcm / sec×cm 2×℃〜0.03calxcm / sec×cm 2·℃的范围内，而散热构件的导热率优选为0.01calxcm / sec×cm 2·℃以下。衬底优选地低于散热构件的衬底。

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