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1. 发明申请

US20050019790A1 Fixation of nucleotide derivatives to solid carrier 审中-公开
标题翻译：核苷酸衍生物固定于固体载体
公开(公告)号：US20050019790A1
公开(公告)日：2005-01-27
申请号：US10777585
申请日：2004-02-12
申请人： Yoshihide Iwaki , Yoshihiko Makino , Hiroshi Shinoki , Satoru Kuhara , Kosuke Tashiro , Shigeru Muta
发明人： Yoshihide Iwaki , Yoshihiko Makino , Hiroshi Shinoki , Satoru Kuhara , Kosuke Tashiro , Shigeru Muta
IPC分类号： G01N33/53 , B01J19/00 , C12M1/00 , C12N15/09 , C40B40/06 , C40B60/14 , G01N33/566 , G01N37/00 , C12Q1/68 , B05D3/00 , C12M1/34
CPC分类号： B01J19/0046 , B01J2219/00351 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： A micro-array for analysis of DNA is prepared by the steps of spotting onto a solid carrier in its predetermined area in which plural reactive groups are fixed an aqueous solution which contains a thickening agent and probe molecules (e.g., DNA fragments) having a group reactive with the reactive group of the solid carrier to produce covalent bonding; spotting onto the solid carrier in a different area having the same reactive groups an aqueous solution (same or different); incubating the aqueous solution-spotted solid carrier to produce the covalent bondings; and washing the solid carrier with an aqueous medium to remove the thickening agent from the solid carrier. An electrostatic bonding can be utilized in place of the covalent bonding.
摘要翻译：用于分析DNA的微阵列通过以下步骤制备：将固定载体固定在其中固定有多个反应性基团的预定区域的固体载体上，所述固定载体含有增稠剂的水溶液和具有基团的探针分子（例如，DNA片段）与固体载体的反应性基团反应产生共价键; 在具有相同反应性基团的不同区域的固体载体上发现水溶液（相同或不同）; 孵育水溶液点固体载体以产生共价键; 并用水介质洗涤固体载体以从固体载体中除去增稠剂。可以使用静电键来代替共价键。

2. 发明申请

US20050266450A1 Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment 审中-公开
标题翻译：用于分析靶核酸片段的方法和用于分析靶核酸片段的试剂盒
公开(公告)号：US20050266450A1
公开(公告)日：2005-12-01
申请号：US11106612
申请日：2005-04-15
申请人： Yoshihiko Makino , Toshihiro Mori , Yoshihide Iwaki
发明人： Yoshihiko Makino , Toshihiro Mori , Yoshihide Iwaki
IPC分类号： C12P19/34 , C12Q1/42 , C12Q1/68 , G01N33/52
CPC分类号： G01N33/523 , C12Q1/42 , C12Q1/6816 , C12Q1/6869 , C12Q2565/625 , C12Q2565/301 , C12Q2521/543 , C12Q2533/101
摘要： An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element for quantifying pyrophosphoric acid. The present invention provides a method for analyzing pyrophosphoric acid generated upon polymerase elongation reaction based on certain nucleotide sequence of a target nucleic acid, a kit for analysis for carrying out the above mentioned method for analysis, and a dry analytical element for quantifying pyrophosphoric acid.
摘要翻译：本发明的目的是提供一种用于分析目标核酸片段的方法，其可以通过使用小型装置简单快速地进行，使用该分析方法分析靶核酸片段的试剂盒和干燥的用于定量焦磷酸的分析元素。本发明提供一种基于靶核酸的某些核苷酸序列分析聚合酶延伸反应产生的焦磷酸的方法，用于进行上述分析方法的分析用试剂盒和定量焦磷酸的干燥分析元素。

3. 发明申请

US20060147991A1 DNA chip and reactive solid carrier 审中-公开
公开(公告)号：US20060147991A1
公开(公告)日：2006-07-06
申请号：US11377058
申请日：2006-03-16
申请人： Hiroshi Shinoki , Yoshihiko Makino , Yumiko Takeshita , Junichi Yamanouchi , Yukio Sudo , Osamu Seshimoto
发明人： Hiroshi Shinoki , Yoshihiko Makino , Yumiko Takeshita , Junichi Yamanouchi , Yukio Sudo , Osamu Seshimoto
IPC分类号： C12Q1/68 , G01N33/53 , C08G63/91 , C12M1/34
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/00497 , B01J2219/00527 , B01J2219/00533 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07C317/08 , C07H21/00 , C40B40/00
摘要： A reactive solid carrier favorably employable for manufacturing DNA chip is composed of a solid carrier and a plurality of vinylsulfonyl groups or their reactive precursors each of which is fixed onto a surface of the solid carrier by covalent bonding via a linking group, and a method for producing a DNA chip is performed by bringing the reactive solid carrier into contact with nucleotide derivatives or their analogues having a reactive group which is reactive with the vinylsulfonyl group or reactive precursor fixed to the solid carrier.

4. 发明申请

US20050095626A1 Method for separation and purification method of nucleic acid 审中-公开
标题翻译：核酸分离纯化方法
公开(公告)号：US20050095626A1
公开(公告)日：2005-05-05
申请号：US10932138
申请日：2004-09-02
申请人： Hiroyuki Komazawa , Yoshihide Iwaki , Yoshihiko Makino , Yoshikazu Amano
发明人： Hiroyuki Komazawa , Yoshihide Iwaki , Yoshihiko Makino , Yoshikazu Amano
IPC分类号： G01N33/53 , B01L3/00 , C12M1/00 , C12N15/09 , C12N15/10 , C12Q1/68 , G01N1/34 , G01N37/00
CPC分类号： C12Q1/6806 , B01J2219/00423 , B01J2219/00641 , B01L3/50255 , C12N15/1017 , C12Q1/6837 , G01N1/34 , C12Q2565/501 , C12Q2563/107 , C12Q2531/113 , C12Q2523/308
摘要： The invention provides a rapid, convenient, and automatable method for extracting a highly pure nucleic acid in order to carry out nucleic acid analysis smoothly with high accuracy in an array method. An analyzing method includes analyzing a nucleic acid by an array method, the nucleic acid being separated and purified by a separation and purification method which includes the steps of (a) to (f) identified in the specification.
摘要翻译：本发明提供一种用于提取高纯度核酸的快速，方便和自动化的方法，以便在阵列方法中以高精度平滑地进行核酸分析。分析方法包括通过阵列法分析核酸，所述核酸通过分离和纯化方法分离和纯化，所述分离和纯化方法包括说明书中确定的步骤（a）至（f）。

5. 发明申请

US20050032080A1 Method for quantifying a target nucleic acid 审中-公开
标题翻译：定量靶核酸的方法
公开(公告)号：US20050032080A1
公开(公告)日：2005-02-10
申请号：US10732312
申请日：2003-12-11
申请人： Yoshihide Iwaki , Yoshihiko Makino
发明人： Yoshihide Iwaki , Yoshihiko Makino
IPC分类号： C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q1/686 , C12Q2537/161 , C12Q2545/107
摘要： An object of the present invention is to provide a method for quantifying a target nucleic acid by PCR. The present invention provides a method for quantifying a target nucleic acid by the polymerase chain reaction utilizing a template nucleic acid comprising a sequence of the target nucleic acid, a pair of primers for amplifying the target nucleic acid, and polymerase, wherein the polymerase chain reaction is conducted in the presence of a pair of competitive primers having a sequence complementary to the sequence of the amplification primer.
摘要翻译：本发明的目的是提供一种通过PCR定量靶核酸的方法。本发明提供了使用包含靶核酸序列的模板核酸，用于扩增靶核酸的一对引物和聚合酶通过聚合酶链式反应来定量靶核酸的方法，其中聚合酶链反应在具有与扩增引物的序列互补的序列的一对竞争性引物的存在下进行。

6. 发明授权

US06589754B1 Immunoassay element and process for immunoassay 失效
标题翻译：免疫测定元件和免疫测定方法
公开(公告)号：US06589754B1
公开(公告)日：2003-07-08
申请号：US08946685
申请日：1997-10-08
申请人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
发明人： Toshikage Hiraoka , Tetsuji Tanimoto , Yoshihiko Makino , Tadashi Ninomiya , Hiroshi Shinoki , Yoshihiro Ashihara , Naofumi Hora , Masashi Ogawa
IPC分类号： G01N3353
CPC分类号： G01N33/54386
摘要： An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.
摘要翻译：一种用于通过测定由高分子聚合物抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析大分子抗原的免疫测定元件。免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。还提供了通过使用免疫测定元件定量分析样品中的大分子抗原的方法。

7. 发明授权

US5882935A Analysis element and method for analyzing glycated hemoglobin content ratio 失效
标题翻译：分析元素及分析糖化血红蛋白含量的方法
公开(公告)号：US5882935A
公开(公告)日：1999-03-16
申请号：US544133
申请日：1995-10-17
申请人： Kikuo Hirai , Hiroshi Shinoki , Masashi Ogawa , Yoshihiko Makino
发明人： Kikuo Hirai , Hiroshi Shinoki , Masashi Ogawa , Yoshihiko Makino
IPC分类号： G01N33/53 , G01N33/52 , G01N33/542 , G01N33/72 , H01L21/302 , H01L21/304 , H01L21/306 , H01L21/76
CPC分类号： G01N33/723 , G01N33/721
摘要： An analysis element for analyzing both amounts of glycated hemoglobin and total hemoglobin in an aqueous liquid sample to determine glycated hemoglobin content ratio in the sample. The element comprises a substrate layer for receiving a reaction mixture after the completion of an immunological reaction between the glycated hemoglobin in the sample and an enzyme-labelled antibody against the glycated hemoglobin, and a reagent layer. The substrate layer contains a non-diffusible substrate which forms a diffusible material in the presence of the enzyme of the enzyme-labelled antibody, the activity of the enzyme being effected relative to the steric hindrance due to the immunological reaction. The reagent layer contains a reagent composition for reacting with the diffusible material to form a dye detectable colorimetrically in a wavelength range which is not effected by an absorption spectrum of the hemoglobin. The total hemoglobin retained in the substrate layer and the dye formed in the reagent layer are colorimetrically analyzed separately, and the glycated hemoglobin content ratio is calculated from respective values. An analysis method using this analysis element is also provided.
摘要翻译：分析元素，用于分析含水液体样品中糖化血红蛋白和总血红蛋白的量，以测定样品中糖化血红蛋白含量的比例。该元件包括用于在样品中的糖化血红蛋白和糖化血红蛋白的酶标记抗体完成后的反应混合物和试剂层的基底层。底物层含有在酶标记抗体的酶存在下形成可扩散材料的非扩散性底物，该酶的活性相对于由于免疫反应引起的空间位阻影响。试剂层含有用于与可扩散材料反应的试剂组合物，以形成可在不受血红蛋白的吸收光谱影响的波长范围内比色测定的染料。分别保留在基材层中的总血红蛋白和形成在试剂层中的染料，并根据各值计算糖化血红蛋白含量比。还提供了使用该分析元素的分析方法。

8. 发明授权

US08511482B2 Method of stably producing microporous membrane and use thereof in method of separating and purifying nucleic acid 有权
标题翻译：稳定生产微孔膜的方法及其在核酸分离纯化方法中的应用
公开(公告)号：US08511482B2
公开(公告)日：2013-08-20
申请号：US11630135
申请日：2005-09-15
申请人： Hideaki Tanaka , Yoshihiko Makino
发明人： Hideaki Tanaka , Yoshihiko Makino
IPC分类号： B01D71/16 , B01D39/16 , B01D39/14 , B01D71/12 , C02F1/44
CPC分类号： B01D67/0013 , B01D15/00 , B01D15/08 , B01D67/0016 , B01D67/0093 , B01D71/12 , B01D2323/08 , B01D2323/22 , B01D2325/02 , B01D2325/022 , B01D2325/12 , B01J20/26 , B01J20/265 , B01J20/28033 , C08J9/28
摘要： A method of producing a porous membrane, the method comprising: casting a polymer solution, in which a polymer is dissolved in a mixture of a good solvent, a poor solvent and a non-solvent, over a support, so as to form a casted polymer solution; drying the casted polymer solution, so as to form a cast film; and subjecting the cast film to a phase separation, wherein the porous membrane is produced under a condition where a temperature of a casted surface is lower than a temperature of the polymer solution, and each of a temperature change of the polymer solution and a temperature change of the casted surface is kept within ±3.0° C.
摘要翻译：一种多孔膜的制造方法，其特征在于，在载体上将聚合物溶解在良溶剂，不良溶剂和非溶剂的混合物中的聚合物溶液中，形成铸塑聚合物溶液; 干燥铸塑聚合物溶液，以形成流延膜; 并对流延膜进行相分离，其中在浇铸表面的温度低于聚合物溶液的温度的条件下制备多孔膜，并且聚合物溶液的温度变化和温度变化的铸造表面保持在±3.0℃

9. 发明授权

US07572578B2 Method for isolating and purifying a nucleic acid 有权
标题翻译：分离和纯化核酸的方法
公开(公告)号：US07572578B2
公开(公告)日：2009-08-11
申请号：US10568101
申请日：2004-09-08
申请人： Toshihiro Mori , Yoshihiko Makino , Rie Hando , Yumiko Takeshita , Hiroko Inomata
发明人： Toshihiro Mori , Yoshihiko Makino , Rie Hando , Yumiko Takeshita , Hiroko Inomata
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译：本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。

10. 发明申请

US20050277152A1 Method for separation and purification of nucleic acid and unit for separation and purification of nucleic acid 审中-公开
标题翻译：核酸分离纯化方法和核酸分离纯化单元
公开(公告)号：US20050277152A1
公开(公告)日：2005-12-15
申请号：US11155469
申请日：2005-06-20
申请人： Yoshihiko Makino , Toshihiro Mori
发明人： Yoshihiko Makino , Toshihiro Mori
IPC分类号： G01N30/00 , C07H21/00 , C12M1/00 , C12N15/09 , C12N15/10 , C12Q1/68 , C12M1/34
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2565/137
摘要： An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation and purification of nucleic acid suitable for implementing this method. The object of the present invention was attained by a method for separation and purification of nucleic acid comprising the steps of adsorbing nucleic acid onto a solid phase and desorbing the nucleic acid from the solid phase, wherein the solid phase comprises a base material in which a graft polymer chain having a hydrophilic group is bonded onto the surface; and a unit for separation and purification of nucleic acid which comprises, in a container having at least two openings, a solid phase of a base material in which a graft polymer chain having a hydrophilic group is bonded onto its surface.
摘要翻译：本发明的目的是提供一种分离和纯化核酸的方法，其具有优异的分离性能，高洗涤效率和易加工性，其使用具有基本上均匀分离能力的大量生产的固相，并且其中程序可以自动化，以及用于分离和纯化适用于实施该方法的核酸的单元。本发明的目的是通过核酸的分离和纯化方法来实现的，其包括将核酸吸附到固相上并从固相中解吸核酸的步骤，其中固相包括基质材料，其中具有亲水基团的接枝聚合物链被结合到表面上; 以及用于分离和纯化核酸的单元，其在具有至少两个开口的容器中包含其中具有亲水基团的接枝聚合物链键合到其表面上的基材的固相。

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