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1. 发明授权

US08511482B2 Method of stably producing microporous membrane and use thereof in method of separating and purifying nucleic acid 有权
标题翻译：稳定生产微孔膜的方法及其在核酸分离纯化方法中的应用
公开(公告)号：US08511482B2
公开(公告)日：2013-08-20
申请号：US11630135
申请日：2005-09-15
申请人： Hideaki Tanaka , Yoshihiko Makino
发明人： Hideaki Tanaka , Yoshihiko Makino
IPC分类号： B01D71/16 , B01D39/16 , B01D39/14 , B01D71/12 , C02F1/44
CPC分类号： B01D67/0013 , B01D15/00 , B01D15/08 , B01D67/0016 , B01D67/0093 , B01D71/12 , B01D2323/08 , B01D2323/22 , B01D2325/02 , B01D2325/022 , B01D2325/12 , B01J20/26 , B01J20/265 , B01J20/28033 , C08J9/28
摘要： A method of producing a porous membrane, the method comprising: casting a polymer solution, in which a polymer is dissolved in a mixture of a good solvent, a poor solvent and a non-solvent, over a support, so as to form a casted polymer solution; drying the casted polymer solution, so as to form a cast film; and subjecting the cast film to a phase separation, wherein the porous membrane is produced under a condition where a temperature of a casted surface is lower than a temperature of the polymer solution, and each of a temperature change of the polymer solution and a temperature change of the casted surface is kept within ±3.0° C.
摘要翻译：一种多孔膜的制造方法，其特征在于，在载体上将聚合物溶解在良溶剂，不良溶剂和非溶剂的混合物中的聚合物溶液中，形成铸塑聚合物溶液; 干燥铸塑聚合物溶液，以形成流延膜; 并对流延膜进行相分离，其中在浇铸表面的温度低于聚合物溶液的温度的条件下制备多孔膜，并且聚合物溶液的温度变化和温度变化的铸造表面保持在±3.0℃

2. 发明申请

US20080061001A1 Method Of Stably Producing Microporous Membrane And Use Thereof In Method Of Separating And Purifying Nucleic Acid 有权
标题翻译：稳定生产微孔膜的方法及其在分离和纯化核酸的方法中的应用
公开(公告)号：US20080061001A1
公开(公告)日：2008-03-13
申请号：US11630135
申请日：2005-09-15
申请人： Hideaki Tanaka , Yoshihiko Makino
发明人： Hideaki Tanaka , Yoshihiko Makino
IPC分类号： B01D71/12 , B01D61/00
CPC分类号： B01D67/0013 , B01D15/00 , B01D15/08 , B01D67/0016 , B01D67/0093 , B01D71/12 , B01D2323/08 , B01D2323/22 , B01D2325/02 , B01D2325/022 , B01D2325/12 , B01J20/26 , B01J20/265 , B01J20/28033 , C08J9/28
摘要： A method of producing a porous membrane, the method comprising: casting a polymer solution, in which a polymer is dissolved in a mixture of a good solvent, a poor solvent and a non-solvent, over a support, so as to form a casted polymer solution; drying the casted polymer solution, so as to form a cast film; and subjecting the cast film to a phase separation, wherein the porous membrane is produced under a condition where a temperature of a casted surface is lower than a temperature of the polymer solution, and each of a temperature change of the polymer solution and a temperature change of the casted surface is kept within ±3.0° C.
摘要翻译：一种多孔膜的制造方法，其特征在于，在载体上将聚合物溶解在良溶剂，不良溶剂和非溶剂的混合物中的聚合物溶液中，形成铸塑聚合物溶液; 干燥铸塑聚合物溶液，以形成流延膜; 并对流延膜进行相分离，其中在浇铸表面的温度低于聚合物溶液的温度的条件下制备多孔膜，并且聚合物溶液的温度变化和温度变化的铸造表面保持在±3.0℃

3. 发明申请

US20060147991A1 DNA chip and reactive solid carrier 审中-公开
公开(公告)号：US20060147991A1
公开(公告)日：2006-07-06
申请号：US11377058
申请日：2006-03-16
申请人： Hiroshi Shinoki , Yoshihiko Makino , Yumiko Takeshita , Junichi Yamanouchi , Yukio Sudo , Osamu Seshimoto
发明人： Hiroshi Shinoki , Yoshihiko Makino , Yumiko Takeshita , Junichi Yamanouchi , Yukio Sudo , Osamu Seshimoto
IPC分类号： C12Q1/68 , G01N33/53 , C08G63/91 , C12M1/34
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/00497 , B01J2219/00527 , B01J2219/00533 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07C317/08 , C07H21/00 , C40B40/00
摘要： A reactive solid carrier favorably employable for manufacturing DNA chip is composed of a solid carrier and a plurality of vinylsulfonyl groups or their reactive precursors each of which is fixed onto a surface of the solid carrier by covalent bonding via a linking group, and a method for producing a DNA chip is performed by bringing the reactive solid carrier into contact with nucleotide derivatives or their analogues having a reactive group which is reactive with the vinylsulfonyl group or reactive precursor fixed to the solid carrier.

4. 发明申请

US20050266450A1 Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment 审中-公开
标题翻译：用于分析靶核酸片段的方法和用于分析靶核酸片段的试剂盒
公开(公告)号：US20050266450A1
公开(公告)日：2005-12-01
申请号：US11106612
申请日：2005-04-15
申请人： Yoshihiko Makino , Toshihiro Mori , Yoshihide Iwaki
发明人： Yoshihiko Makino , Toshihiro Mori , Yoshihide Iwaki
IPC分类号： C12P19/34 , C12Q1/42 , C12Q1/68 , G01N33/52
CPC分类号： G01N33/523 , C12Q1/42 , C12Q1/6816 , C12Q1/6869 , C12Q2565/625 , C12Q2565/301 , C12Q2521/543 , C12Q2533/101
摘要： An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element for quantifying pyrophosphoric acid. The present invention provides a method for analyzing pyrophosphoric acid generated upon polymerase elongation reaction based on certain nucleotide sequence of a target nucleic acid, a kit for analysis for carrying out the above mentioned method for analysis, and a dry analytical element for quantifying pyrophosphoric acid.
摘要翻译：本发明的目的是提供一种用于分析目标核酸片段的方法，其可以通过使用小型装置简单快速地进行，使用该分析方法分析靶核酸片段的试剂盒和干燥的用于定量焦磷酸的分析元素。本发明提供一种基于靶核酸的某些核苷酸序列分析聚合酶延伸反应产生的焦磷酸的方法，用于进行上述分析方法的分析用试剂盒和定量焦磷酸的干燥分析元素。

5. 发明授权

US07572578B2 Method for isolating and purifying a nucleic acid 有权
标题翻译：分离和纯化核酸的方法
公开(公告)号：US07572578B2
公开(公告)日：2009-08-11
申请号：US10568101
申请日：2004-09-08
申请人： Toshihiro Mori , Yoshihiko Makino , Rie Hando , Yumiko Takeshita , Hiroko Inomata
发明人： Toshihiro Mori , Yoshihiko Makino , Rie Hando , Yumiko Takeshita , Hiroko Inomata
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译：本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。

6. 发明申请

US20050277152A1 Method for separation and purification of nucleic acid and unit for separation and purification of nucleic acid 审中-公开
标题翻译：核酸分离纯化方法和核酸分离纯化单元
公开(公告)号：US20050277152A1
公开(公告)日：2005-12-15
申请号：US11155469
申请日：2005-06-20
申请人： Yoshihiko Makino , Toshihiro Mori
发明人： Yoshihiko Makino , Toshihiro Mori
IPC分类号： G01N30/00 , C07H21/00 , C12M1/00 , C12N15/09 , C12N15/10 , C12Q1/68 , C12M1/34
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2565/137
摘要： An object of the present invention is to provide a method for separation and purification of nucleic acid, which has excellent separating properties, high washing efficiency, and easy processability, which uses mass-producible solid phases with substantially uniform separating capacities, and wherein procedures can be automated, and a unit for separation and purification of nucleic acid suitable for implementing this method. The object of the present invention was attained by a method for separation and purification of nucleic acid comprising the steps of adsorbing nucleic acid onto a solid phase and desorbing the nucleic acid from the solid phase, wherein the solid phase comprises a base material in which a graft polymer chain having a hydrophilic group is bonded onto the surface; and a unit for separation and purification of nucleic acid which comprises, in a container having at least two openings, a solid phase of a base material in which a graft polymer chain having a hydrophilic group is bonded onto its surface.
摘要翻译：本发明的目的是提供一种分离和纯化核酸的方法，其具有优异的分离性能，高洗涤效率和易加工性，其使用具有基本上均匀分离能力的大量生产的固相，并且其中程序可以自动化，以及用于分离和纯化适用于实施该方法的核酸的单元。本发明的目的是通过核酸的分离和纯化方法来实现的，其包括将核酸吸附到固相上并从固相中解吸核酸的步骤，其中固相包括基质材料，其中具有亲水基团的接枝聚合物链被结合到表面上; 以及用于分离和纯化核酸的单元，其在具有至少两个开口的容器中包含其中具有亲水基团的接枝聚合物链键合到其表面上的基材的固相。

7. 发明授权

US06399305B1 Protection of partial complementary nucleic acid fragment using a electroconductive chip and intercalator 有权
标题翻译：使用导电芯片和嵌入剂保护部分互补核酸片段
公开(公告)号：US06399305B1
公开(公告)日：2002-06-04
申请号：US09588950
申请日：2000-06-07
申请人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
发明人： Yoshihiko Makino , Yoshihiko Abe , Makoto Takagi , Shigeori Takenaka , Kenichi Yamashita , Masashi Ogawa
IPC分类号： C12Q168
CPC分类号： C12Q1/6825 , Y10T436/143333 , C12Q2563/173 , C12Q2563/113
摘要： A method of analyzing a nucleic acid fragment sample to judge whether the nucleic acid fragment sample is uncomplementary, partly complementary or complementary to a DNA fragment in its specific base sequence is conducted by the steps of: bringing an aqueous solution of the nucleic acid fragment sample into contact with a DNA chip having an electroconductive substrate and the DNA fragment fixed onto the substrate in the presence of an electrochemical thread intercalator; measuring an electric current flowing from or to the electroconductive substrate along the DNA fragment under application of a potential to the substrate; and comparing the electric current measured above with a referential electric current which is prepared employing a DNA chip equivalent to the above DNA chip, the intercalator, and an aqueous solution of a nucleic acid fragment which is complementary to the DNA fragment of the DNA chip.
摘要翻译：分析核酸片段样品以判断核酸片段样品是否与其特异性碱基序列中的DNA片段不互补，部分互补或互补的方法通过以下步骤进行：使核酸片段样品的水溶液与具有导电性基体的DNA芯片接触，并且在电化学线性嵌入剂的存在下固定在基材上的DNA片段;测量在施加电位下沿着DNA片段向基片流向或流到导电基底的电流 ; 并使用与上述DNA芯片相当的DNA芯片，嵌入剂和与DNA芯片的DNA片段互补的核酸片段的水溶液制备的参考电流来比较上述电流。

8. 发明授权

US5393493A Analytical element for whole blood 失效
标题翻译：全血分析元素
公开(公告)号：US5393493A
公开(公告)日：1995-02-28
申请号：US993459
申请日：1992-12-15
申请人： Yoshihiko Makino , Kaoru Terashima , Toru Kitani , Naofumi Hora
发明人： Yoshihiko Makino , Kaoru Terashima , Toru Kitani , Naofumi Hora
IPC分类号： G01N31/22 , B01L3/00 , B01L99/00 , C12Q1/48 , C12Q1/60 , G01N21/78 , G01N33/49 , G01N33/52 , G01N33/92
CPC分类号： G01N33/525 , C12Q1/48 , C12Q1/60
摘要： An integral multilayer analytical element in which a first fibrous porous layer, a nonfibrous porous layer, and a second fibrous porous layer superposed in this order on a water-impermeable light-transmissive support the first fibrous porous-layer having a larger pore size than the nonfibrous porous layer. The above three porous layers are integrally laminated to each other substantially closely by an adhesive discontinuously disposed so as to form microspaces continuing through from one layer to the next so as not to interfere with the approximately uniform permeation of liquid. A reagent composition which produces an optically detectable change in the presence of an analyte is incorporated in at least one of said three porous layers. When a nonporous reagent layer is incorporated on the support in the above analytical element, the reagent composition is incorporated in the nonporous reagent layer. By using the analytical element of the invention, the analytical values of various analytes of blood samples can be obtained independently of hematocrit values from whole blood samples and blood plasma samples in the range of the hematocrit values of 0% to 55%.
摘要翻译：一种整体的多层分析元件，其中第一纤维多孔层，非纤维多孔层和第二纤维多孔层依次叠加在不透水的透光性上，支撑第一纤维多孔层，其孔径大于非纤维多孔层。上述三个多孔层通过不连续地布置的粘合剂基本上紧密地彼此层压，以便形成从一层到下一层的连续通过的微孔，以便不干扰液体的大致均匀渗透。在分析物存在下产生可光学检测的变化的试剂组合物结合在所述三个多孔层中的至少一个中。当在上述分析元件中的载体上并入无孔试剂层时，将试剂组合物并入无孔试剂层。通过使用本发明的分析元件，可以在血细胞比容值为0％至55％的范围内，独立于来自全血样品和血浆样品的血细胞比容值而独立地获得血液样品的各种分析物的分析值。

9. 发明授权

US07682818B2 Apparatus for separating and purifying nucleic acid and method for separating and purifying nucleic acid 有权
标题翻译：用于分离和纯化核酸的装置和分离和纯化核酸的方法
公开(公告)号：US07682818B2
公开(公告)日：2010-03-23
申请号：US10808411
申请日：2004-03-25
申请人： Toshihiro Mori , Yoshihiko Makino
发明人： Toshihiro Mori , Yoshihiko Makino
IPC分类号： C12M1/34 , C12Q1/68 , C12P19/34 , G01N15/06 , C07H21/02
CPC分类号： C12N15/1003 , B01L3/0275 , B01L2200/0631 , B01L2300/0681 , B01L2400/0478 , B01L2400/0605 , G01N1/405
摘要： The present invention provides an apparatus for separating and purifying nucleic acids, which comprises: a cylindrical syringe having a leading end part in which a first opening part is formed, a base end part in which a second opening part is formed and an accommodation part between said first opening part and second opening part, the accommodation part being able to hold liquid therein; and a solid phase-holding member connected to said leading end part, a flow hole being formed at the leading end side of the solid phase-holding member; wherein a solid phase comprised of an organic polymer having a hydroxyl group on the surface thereof is accommodated in said solid phase-holding member, the solid phase being able to adsorb and desorb nucleic acids in a sample solution; and wherein a pressure sensor capable of detecting the pressure in the accommodation part is connected.
摘要翻译：本发明提供了一种分离和纯化核酸的装置，包括：圆柱形注射器，其具有形成有第一开口部的前端部，形成有第二开口部的基端部和第二开口部之间的容纳部，所述第一开口部分和第二开口部分，所述容纳部分能够将液体保持在其中; 以及固相保持构件，其连接到所述前端部，流动孔形成在所述固相保持构件的前端侧; 其中由表面具有羟基的有机聚合物构成的固相被容纳在所述固相保持部件中，所述固相能够吸附和解吸样品溶液中的核酸; 并且其中连接有能够检测所述容纳部中的压力的压力传感器。

10. 发明申请

US20080275228A1 Method and Apparatus of Automatically Isolating and Purifying Nucleic Acid 有权
标题翻译：自动分离和纯化核酸的方法和装置
公开(公告)号：US20080275228A1
公开(公告)日：2008-11-06
申请号：US11547093
申请日：2005-03-28
申请人： Toshihiro Mori , Yoshihiko Makino
发明人： Toshihiro Mori , Yoshihiko Makino
IPC分类号： C07H21/00 , C12M1/00
CPC分类号： C12N15/1006 , B01D15/14 , B01D15/163 , B01J20/26 , B01J20/262 , B01J20/265 , B01J20/28014 , B01J20/28033 , B01J20/28054 , C12N15/1017 , G01N30/32
摘要： A method of automatically isolating and purifying nucleic acid from a nucleic acid-containing specimen is provided, the method comprising: injecting a liquid into a cartridge for isolation and purification of a nucleic acid including at least two openings from one opening of the at least two openings, in which the cartridge includes a container having the at least two openings and containing a nucleic acid-adsorbent solid phase; passing the liquid through the nucleic acid-adsorbent solid phase by a pressure difference generated by a pressure generation means for generating a pressure difference between the inside and outside of the container; and discharging the liquid from the other opening of the container to the outside of the container by a pressure difference generated by the pressure generation means, wherein a pressure generated in the inside of the container by the pressure generation means is measured, a pressure change velocity and a pressure change acceleration are calculated on the basis of the value of the measured pressure, and the timing of completion of discharge of the liquid from the container is determined by use of a temporal change pattern of at least one of the measured pressure, the pressure change velocity and the pressure change acceleration.
摘要翻译：提供了一种从含核酸样品自动分离和纯化核酸的方法，所述方法包括：将液体注入用于分离和纯化核酸的液体，所述核酸包括至少两个开口的至少两个开口开口，其中所述盒包括具有所述至少两个开口并且包含核酸 - 吸附剂固相的容器; 通过由压力产生装置产生的压力差使液体通过核酸 - 吸附剂固相，用于产生容器内部和外部之间的压力差; 并且通过由压力产生装置产生的压力差将液体从容器的另一个开口排出到容器的外部，其中测量由压力产生装置在容器内部产生的压力，压力变化速度并且基于测量的压力的值来计算压力变化加速度，并且通过使用测量压力中的至少一个的时间变化模式来确定来自容器的液体的排出的完成时间，压力变化速度和压力变化加速度。

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