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1. 发明申请

US20050095626A1 Method for separation and purification method of nucleic acid 审中-公开
标题翻译：核酸分离纯化方法
公开(公告)号：US20050095626A1
公开(公告)日：2005-05-05
申请号：US10932138
申请日：2004-09-02
申请人： Hiroyuki Komazawa , Yoshihide Iwaki , Yoshihiko Makino , Yoshikazu Amano
发明人： Hiroyuki Komazawa , Yoshihide Iwaki , Yoshihiko Makino , Yoshikazu Amano
IPC分类号： G01N33/53 , B01L3/00 , C12M1/00 , C12N15/09 , C12N15/10 , C12Q1/68 , G01N1/34 , G01N37/00
CPC分类号： C12Q1/6806 , B01J2219/00423 , B01J2219/00641 , B01L3/50255 , C12N15/1017 , C12Q1/6837 , G01N1/34 , C12Q2565/501 , C12Q2563/107 , C12Q2531/113 , C12Q2523/308
摘要： The invention provides a rapid, convenient, and automatable method for extracting a highly pure nucleic acid in order to carry out nucleic acid analysis smoothly with high accuracy in an array method. An analyzing method includes analyzing a nucleic acid by an array method, the nucleic acid being separated and purified by a separation and purification method which includes the steps of (a) to (f) identified in the specification.
摘要翻译：本发明提供一种用于提取高纯度核酸的快速，方便和自动化的方法，以便在阵列方法中以高精度平滑地进行核酸分析。分析方法包括通过阵列法分析核酸，所述核酸通过分离和纯化方法分离和纯化，所述分离和纯化方法包括说明书中确定的步骤（a）至（f）。

2. 发明申请

US20050032080A1 Method for quantifying a target nucleic acid 审中-公开
标题翻译：定量靶核酸的方法
公开(公告)号：US20050032080A1
公开(公告)日：2005-02-10
申请号：US10732312
申请日：2003-12-11
申请人： Yoshihide Iwaki , Yoshihiko Makino
发明人： Yoshihide Iwaki , Yoshihiko Makino
IPC分类号： C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q1/686 , C12Q2537/161 , C12Q2545/107
摘要： An object of the present invention is to provide a method for quantifying a target nucleic acid by PCR. The present invention provides a method for quantifying a target nucleic acid by the polymerase chain reaction utilizing a template nucleic acid comprising a sequence of the target nucleic acid, a pair of primers for amplifying the target nucleic acid, and polymerase, wherein the polymerase chain reaction is conducted in the presence of a pair of competitive primers having a sequence complementary to the sequence of the amplification primer.
摘要翻译：本发明的目的是提供一种通过PCR定量靶核酸的方法。本发明提供了使用包含靶核酸序列的模板核酸，用于扩增靶核酸的一对引物和聚合酶通过聚合酶链式反应来定量靶核酸的方法，其中聚合酶链反应在具有与扩增引物的序列互补的序列的一对竞争性引物的存在下进行。

3. 发明申请

US20050019790A1 Fixation of nucleotide derivatives to solid carrier 审中-公开
标题翻译：核苷酸衍生物固定于固体载体
公开(公告)号：US20050019790A1
公开(公告)日：2005-01-27
申请号：US10777585
申请日：2004-02-12
申请人： Yoshihide Iwaki , Yoshihiko Makino , Hiroshi Shinoki , Satoru Kuhara , Kosuke Tashiro , Shigeru Muta
发明人： Yoshihide Iwaki , Yoshihiko Makino , Hiroshi Shinoki , Satoru Kuhara , Kosuke Tashiro , Shigeru Muta
IPC分类号： G01N33/53 , B01J19/00 , C12M1/00 , C12N15/09 , C40B40/06 , C40B60/14 , G01N33/566 , G01N37/00 , C12Q1/68 , B05D3/00 , C12M1/34
CPC分类号： B01J19/0046 , B01J2219/00351 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00689 , B01J2219/00722 , C40B40/06 , C40B60/14
摘要： A micro-array for analysis of DNA is prepared by the steps of spotting onto a solid carrier in its predetermined area in which plural reactive groups are fixed an aqueous solution which contains a thickening agent and probe molecules (e.g., DNA fragments) having a group reactive with the reactive group of the solid carrier to produce covalent bonding; spotting onto the solid carrier in a different area having the same reactive groups an aqueous solution (same or different); incubating the aqueous solution-spotted solid carrier to produce the covalent bondings; and washing the solid carrier with an aqueous medium to remove the thickening agent from the solid carrier. An electrostatic bonding can be utilized in place of the covalent bonding.
摘要翻译：用于分析DNA的微阵列通过以下步骤制备：将固定载体固定在其中固定有多个反应性基团的预定区域的固体载体上，所述固定载体含有增稠剂的水溶液和具有基团的探针分子（例如，DNA片段）与固体载体的反应性基团反应产生共价键; 在具有相同反应性基团的不同区域的固体载体上发现水溶液（相同或不同）; 孵育水溶液点固体载体以产生共价键; 并用水介质洗涤固体载体以从固体载体中除去增稠剂。可以使用静电键来代替共价键。

4. 发明申请

US20050266450A1 Method for analyzing a target nucleic acid fragment and a kit for analyzing a target nucleic acid fragment 审中-公开
标题翻译：用于分析靶核酸片段的方法和用于分析靶核酸片段的试剂盒
公开(公告)号：US20050266450A1
公开(公告)日：2005-12-01
申请号：US11106612
申请日：2005-04-15
申请人： Yoshihiko Makino , Toshihiro Mori , Yoshihide Iwaki
发明人： Yoshihiko Makino , Toshihiro Mori , Yoshihide Iwaki
IPC分类号： C12P19/34 , C12Q1/42 , C12Q1/68 , G01N33/52
CPC分类号： G01N33/523 , C12Q1/42 , C12Q1/6816 , C12Q1/6869 , C12Q2565/625 , C12Q2565/301 , C12Q2521/543 , C12Q2533/101
摘要： An object of the present invention is to provide a method for analyzing a target nucleic acid fragment which can be simply and swiftly carried out by using a small apparatus, a kit for analyzing a target nucleic acid fragment using the method for analysis, and a dry analytical element for quantifying pyrophosphoric acid. The present invention provides a method for analyzing pyrophosphoric acid generated upon polymerase elongation reaction based on certain nucleotide sequence of a target nucleic acid, a kit for analysis for carrying out the above mentioned method for analysis, and a dry analytical element for quantifying pyrophosphoric acid.
摘要翻译：本发明的目的是提供一种用于分析目标核酸片段的方法，其可以通过使用小型装置简单快速地进行，使用该分析方法分析靶核酸片段的试剂盒和干燥的用于定量焦磷酸的分析元素。本发明提供一种基于靶核酸的某些核苷酸序列分析聚合酶延伸反应产生的焦磷酸的方法，用于进行上述分析方法的分析用试剂盒和定量焦磷酸的干燥分析元素。

5. 发明申请

US20120179384A1 METHOD FOR ANALYZING NUCLEIC ACID MUTATION USING ARRAY COMPARATIVE GENOMIC HYBRIDIZATION TECHNIQUE 审中-公开
标题翻译：使用阵列比较基因组杂交技术分析核酸突变的方法
公开(公告)号：US20120179384A1
公开(公告)日：2012-07-12
申请号：US13395509
申请日：2010-09-09
申请人： Masayuki Kuramitsu , Yoshihide Iwaki , Dai Ujihara
发明人： Masayuki Kuramitsu , Yoshihide Iwaki , Dai Ujihara
IPC分类号： G06F19/20
CPC分类号： C12Q1/6837 , C12Q2600/156 , G16B25/00
摘要： There is provided a method for analyzing nucleic acid mutation using an array comparative genomic hybridization technique, which reduces the false positive and the false negative and improves the reliability of the analysis results. A method for analyzing nucleic acid mutation using array comparative genomic hybridization technique comprising: [(a) a step of bringing a plurality of labeled sample nucleic acids one by one into contact with the plural same probe nucleic acid sets], [(b) a step of obtaining label intensities], [(c) a step of determining whether each piece of comparison values falls within a prescribed numerical value range or not], and [(d) a step of comparing whether the number of the comparison values exceeds a prescribed number or not and, in the case of exceeding the prescribed number, judging the spot positive].
摘要翻译：提供了使用阵列比较基因组杂交技术分析核酸突变的方法，其减少了假阳性和假阴性，并提高了分析结果的可靠性。使用阵列比较基因组杂交技术分析核酸突变的方法，包括：（a）使多个标记的样品核酸逐个与多个相同的探针核酸组相接触的步骤]，[（b）获得标签强度的步骤]，[（c）确定每个比较值是否落在规定的数值范围内的步骤]，[（d）比较比较值的数量是否超过在规定数量的情况下，判定现场为正]。

6. 发明申请

US20100047794A1 METHOD FOR DISCRIMINATING BETWEEN NUCLEOTIDE SEQUENCES OF NUCLEIC ACIDS 有权
标题翻译：用于分离核酸核苷酸序列的方法
公开(公告)号：US20100047794A1
公开(公告)日：2010-02-25
申请号：US12471817
申请日：2009-05-26
申请人： Hayato Miyoshi , Yoshihide Iwaki , Toshihiro Mori
发明人： Hayato Miyoshi , Yoshihide Iwaki , Toshihiro Mori
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q1/6858 , C12Q2531/119 , C12Q2531/101
摘要： It is an object of the present invention to provide a method for discriminating between nucleic acid sequences with high accuracy by utilizing a method for specifically amplifying nucleic acid sequences under isothermal conditions. The present invention provides a method for discriminating between the nucleotide sequence of a first nucleic acid and the nucleotide sequence of a second nucleic acid by a nucleic acid amplification method that is performed under substantially isothermal conditions, wherein (1) at least one type of oligonucleotide (hereinafter “primer”) substantially complementary to the first nucleic acid, and (2) at least one type of oligonucleic acid (hereinafter “mask oligo”) that is designed such that it hybridizes to the nucleotide sequence portions of the first nucleic acid and the second nucleic acid to be discriminated, such that it is more complementary to the second nucleic acid than to the first nucleic acid, and such that it does not become an origin of an elongation reaction with polymerase, are used, the method being characterized in that a portion of the primer and a portion of the mask oligo hybridize to the same regions on the first nucleic acid and the second nucleic acid.
摘要翻译：本发明的目的是提供一种通过利用在等温条件下特异性扩增核酸序列的方法来高精度地区分核酸序列的方法。本发明提供了通过在基本上等温条件下进行的核酸扩增方法来区分第一核酸的核苷酸序列和第二核酸的核苷酸序列的方法，其中（1）至少一种类型的寡核苷酸（以下简称为“引物”），和（2）至少一种低聚核酸（以下称为“掩模寡核苷酸”），其被设计为与第一核酸的核苷酸序列部分杂交，要被鉴别的第二核酸，使得其比第一核酸与第二核酸更互补，并且使其不成为与聚合酶的延伸反应的起源，所述方法的特征在于引物的一部分和掩模寡核苷酸的一部分与第一核酸和第二核酸上的相同区域杂交。

7. 发明授权

US07410613B2 Humoral testing apparatus 失效
标题翻译：体液检测仪
公开(公告)号：US07410613B2
公开(公告)日：2008-08-12
申请号：US10397376
申请日：2003-03-27
申请人： Yoshihide Iwaki , Kentarou Nakamura , Hideaki Tanaka , Yoshiki Sakaino , Kaoru Terashima , Hitoshi Shimizu
发明人： Yoshihide Iwaki , Kentarou Nakamura , Hideaki Tanaka , Yoshiki Sakaino , Kaoru Terashima , Hitoshi Shimizu
IPC分类号： G01N21/78
CPC分类号： G01N21/78 , G01N21/5907
摘要： A humoral testing apparatus in which a measuring light is irradiated to a reagent area forming a color as a result of a reaction and an optical density of the reagent area is detected by a detecting operation of light intensity of the reflected light. The humoral testing apparatus enables a humoral test to be performed accurately in cases where nonuniformity occurs with the reaction of a reagent with a humoral sample within the reagent area, or in cases where fine dust is present within each detecting spot in the reagent areas. The light reflected from the reagent areas is detected with a two-dimensional photodetector. The independent light intensity detecting operations are performed with respect to the subareas of the reagent area of a reagent layer. A photo detection signal S, which represents the intensity of the reflected light is statistically processed with a signal processing section 51.
摘要翻译：通过反射光的光强度的检测操作来检测其中测量光照射到由于反应而形成颜色的试剂区域和试剂区域的光密度的体液测试装置。体液检测装置能够在试剂区域内的试剂与体液样品的反应发生不均匀的情况下，或者在试剂区域的每个检测点内存在微细粉尘的情况下，能够准确地进行体液检查。用二维光电检测器检测从试剂区反射的光。针对试剂层的试剂区域的子区域进行独立的光强度检测动作。用信号处理部分51对表示反射光强度的光检测信号S进行统计处理。

8. 发明申请

US20080152543A1 TEMPERATURE REGULATION METHOD OF MICROFLUIDIC CHIP, SAMPLE ANALYSIS SYSTEM AND MICROFLUIDIC CHIP 审中-公开
标题翻译：微流控芯片的温度调节方法，样品分析系统和微流控芯片
公开(公告)号：US20080152543A1
公开(公告)日：2008-06-26
申请号：US11943370
申请日：2007-11-20
申请人： Hideyuki KARAKI , Tatsuo Fujikura , Akira Wakabayashi , Yoshihide Iwaki
发明人： Hideyuki KARAKI , Tatsuo Fujikura , Akira Wakabayashi , Yoshihide Iwaki
IPC分类号： G01N21/64 , B01J19/00 , G05D23/00 , G05D23/13
CPC分类号： G05D23/1919 , B01L3/5027 , B01L3/502746 , B01L7/52 , B01L2200/0684 , B01L2200/142 , B01L2200/147 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2300/1822 , B01L2300/1827 , B01L2400/0487 , G01N21/0332 , G01N21/05 , G01N21/272 , G01N21/6486 , G01N2021/0325 , G01N2021/0346
摘要： A temperature regulation method of a microfluidic chip for controlling a temperature of a liquid accumulated in a reaction channel formed in a flat plate of the microfluidic chip, the method includes: heating the reaction channel to a reaction temperature; and at the same time as the reaction channel is heated to the reaction temperature, keeping a first channel communicating with one end of the reaction channel and a second channel communicating with an opposite end of the reaction channel at the same temperature different from the reaction temperature by heating or cooling.
摘要翻译：一种微流控芯片的温度调节方法，用于控制积聚在形成于微流体芯片的平板中的反应通道中的液体的温度，该方法包括：将反应通道加热至反应温度; 并且在反应通道被加热到反应温度的同时，保持与反应通道的一端连通的第一通道和与反应通道的相反端在与反应温度不同的相同温度下连通的第二通道通过加热或冷却。

9. 发明申请

US20080044921A1 Primers used in novel gene amplification method 审中-公开
标题翻译：引物用于新基因扩增方法
公开(公告)号：US20080044921A1
公开(公告)日：2008-02-21
申请号：US11822271
申请日：2007-07-03
申请人： Yoshihide Iwaki , Toshihiro Mori
发明人： Yoshihide Iwaki , Toshihiro Mori
IPC分类号： G01N33/48 , C07H21/00
CPC分类号： C12Q1/6853 , C12Q1/6858 , Y10T436/143333 , C12Q2525/301 , C12Q2525/161
摘要： A primer for amplifying a target nucleic acid sequence, comprises: a sequence region (a) complementary to a sequence region (a′) in the target nucleic acid sequence; and a sequence region (b) having a sequence complementary to a partial sequence of the sequence region (a), in this order from a 3′ terminal side to a 5′ terminal side of the primer.
摘要翻译：用于扩增靶核酸序列的引物包括：与靶核酸序列中的序列区（a'）互补的序列区（a）; 和具有与序列区（a）的部分序列互补的序列的序列区（b），从该引物的3'末端侧至5'末端侧。

10. 发明申请

US20090170096A1 NUCLEIC ACID AMPLIFICATION METHOD 有权
标题翻译：核酸扩增方法
公开(公告)号：US20090170096A1
公开(公告)日：2009-07-02
申请号：US12179098
申请日：2008-07-24
申请人： Hayato MIYOSHI , Yoshihide Iwaki , Toshihiro Mori
发明人： Hayato MIYOSHI , Yoshihide Iwaki , Toshihiro Mori
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6853 , C12Q2531/119 , C12Q2527/125 , C12Q2521/101
摘要： An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified substantially isothermally using oligonucleotide primers and DNA polymerase capable of strand displacement. The present invention provides a nucleic acid amplification method which comprises performing substantially isothermal incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, a divalent cation, at least 0.01% or more surfactant, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment.
摘要翻译：本发明要实现的目的是提供一种使用寡核苷酸引物和能够进行链置换的DNA聚合酶基本上等温扩增核酸的核酸扩增方法。本发明提供了一种核酸扩增方法，其包括对含有至少一种类型的脱氧核苷酸三磷酸的反应溶液进行基本上恒温培养，至少一种具有链置换活性的DNA聚合酶，二价阳离子，至少0.01％或更多表面活性剂，至少两种类型的寡核苷酸引物和核酸片段作为模板，从而进行从引物的3'末端引发并因此扩增核酸片段的聚合酶反应。

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