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1. 发明授权

US06645766B2 Shuttle vectors 失效
公开(公告)号：US06645766B2
公开(公告)日：2003-11-11
申请号：US10043074
申请日：2002-01-07
申请人： Ying Luo , Pei Wen Yu , James Lorens
发明人： Ying Luo , Pei Wen Yu , James Lorens
IPC分类号： C12N1564
CPC分类号： C12N15/1082 , C12N15/1034 , C12N15/70 , C12N15/81
摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

2. 发明授权

US06391582B2 Shuttle vectors 有权
标题翻译：班车向量
公开(公告)号：US06391582B2
公开(公告)日：2002-05-21
申请号：US09208827
申请日：1998-12-09
申请人： Ying Luo , Pei Wen Yu , James Lorens
发明人： Ying Luo , Pei Wen Yu , James Lorens
IPC分类号： C12P2102
CPC分类号： C12N15/1082 , C12N15/1034 , C12N15/70 , C12N15/81
摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.
摘要翻译：本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。还提供了一种方法，其中用本文提供的穿梭载体转化酵母。将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物插入载体。然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

3. 发明授权

US06280937B1 Shuttle vectors 有权
标题翻译：班车向量
公开(公告)号：US06280937B1
公开(公告)日：2001-08-28
申请号：US09133944
申请日：1998-08-14
申请人： Ying Luo , Pei Wen Yu , James Lorens
发明人： Ying Luo , Pei Wen Yu , James Lorens
IPC分类号： C12Q168
CPC分类号： C12N15/1082 , C12N15/70 , C12N15/81
摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.
摘要翻译：本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。还提供了一种方法，其中用本文提供的穿梭载体转化酵母。将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物体插入载体中。然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

4. 发明授权

US06623980B1 Exo1 and Exo2, exocytotic proteins 有权
标题翻译： Exo1和Exo2，胞吐蛋白
公开(公告)号：US06623980B1
公开(公告)日：2003-09-23
申请号：US09255920
申请日：1999-02-23
申请人： Joseph Fisher , James Lorens , David Anderson , Ying Luo , Chao Bai (Betty) Huang , Mary Shen
发明人： Joseph Fisher , James Lorens , David Anderson , Ying Luo , Chao Bai (Betty) Huang , Mary Shen
IPC分类号： G01N33566
CPC分类号： C07K14/4703
摘要： The present invention is directed to novel exocytotic polypeptides, such as Exo1 and Exo2 polypeptides and related molecules, which have an inhibitory effect on exocytosis and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are method for identifying novel compositions which mediate exocytotic polypeptide bioactivity, and the use of such compositions in diagnosis and treatment of disease.
摘要翻译：本发明涉及新颖的胞吐多肽，例如Exo1和Exo2多肽及相关分子，其对胞吐作用具有抑制作用，以及编码这些多肽的核酸分子。本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法发明。本发明还提供了鉴定介导胞吐多肽生物活性的新组合物的方法，以及这些组合物在疾病诊断和治疗中的应用。

5. 发明申请

US20060264392A1 Modulators of angiogenesis 有权
标题翻译：血管生成调节剂
公开(公告)号：US20060264392A1
公开(公告)日：2006-11-23
申请号：US10525573
申请日：2003-09-02
申请人： James Lorens , Jakob Bogenberger , Sacha Holland , Weiduan Xu
发明人： James Lorens , Jakob Bogenberger , Sacha Holland , Weiduan Xu
IPC分类号： A61K48/00 , A61K38/54 , C12Q1/68 , G01N33/574
CPC分类号： C12Q1/6883 , C07K14/4702 , C12Q2600/158
摘要： The present invention relates to regulation of angiogenesis. More particularly, the present invention is directed to nucleic acids encoding “angiogenesis regulatory proteins and nucleic acids” which are involved in modulation of angiogenesis. The invention further relates to methods for identifying and using agents, including small organic molecules, antibodies, peptides, cyclic peptides, nucleic acids, antisense nucleic adds, RNAi, and ribozymes, that modulate angiogenesis via modulation of angiogenesis regulatory proteins and nucleic acids; as well as to the use of expression profiles and compositions in diagnosis and therapy of diseases related to angiogenesis.
摘要翻译：本发明涉及血管发生的调节。更具体地，本发明涉及编码血管发生调节的“血管发生调节蛋白和核酸”的核酸。本发明还涉及用于鉴定和使用通过调节血管生成调节蛋白和核酸调节血管生成的试剂，包括小有机分子，抗体，肽，环肽，核酸，反义核酸添加物，RNAi和核酶的试剂; 以及在血管生成相关疾病的诊断和治疗中使用表达谱和组合物。

6. 发明授权

US06461813B2 Multiparameter FACS assays to detect alterations in cell cycle regulation 有权
标题翻译：多参数FACS测定法检测细胞周期调节的变化
公开(公告)号：US06461813B2
公开(公告)日：2002-10-08
申请号：US09157748
申请日：1998-09-21
申请人： James Lorens
发明人： James Lorens
IPC分类号： C12Q168
CPC分类号： G01N33/5011 , G01N33/5008 , G01N33/5014 , G01N33/56966 , G01N2333/43595 , G01N2333/4718
摘要： The invention related to novel methods of detecting alterations in cell cycle regulation in a cell or a cell population and screening for agents capable of modulating cell cycle regulation through the use of multiparameter assays and a fluorescence-activated cell sorter (FACS) machine.
摘要翻译：本发明涉及检测细胞或细胞群体中细胞周期调节变化的新方法，以及通过使用多参数测定法和荧光激活细胞分选仪（FACS）机器筛选能够调节细胞周期调节的试剂。

7. 发明授权

US08735064B2 Methods for creating and identifying functional RNA interference elements 有权
标题翻译：用于创建和鉴定功能性RNA干扰元件的方法
公开(公告)号：US08735064B2
公开(公告)日：2014-05-27
申请号：US12735222
申请日：2008-12-23
申请人： David Micklem , James Lorens
发明人： David Micklem , James Lorens
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02
CPC分类号： C12N15/1086 , C12N15/10 , C12N15/111 , C12N15/64 , C12N15/66 , C12N2310/14 , C12N2320/10 , C12N2330/30 , C12Q1/6897
摘要： The invention relates to the control of gene expression. Specifically, the invention provides compositions and methods for the production and use of recombinant nucleic acid molecules that have the ability to specifically downregulate an expressed target gene in vivo. In some aspects, the invention provides methods for producing a hairpin DNA molecule where part of the molecule is derived from an mRNA that is a target for a small interfering RNA (siRNA) derived from the hairpin. In other aspects, the invention provides synthetic hairpin adapter oligonucleotides that are used in the construction of siRNA-producing cassettes. In other aspects, the invention provides methods for testing for the presence or absence of specific inhibitory activity of an RNAi trigger molecule, and in still other aspects, the invention provides methods for identifying an active RNAi trigger molecule from a library of RNAi trigger molecules. In still other aspects, the invention provides methods for identifying a polynucleotide from a plurality of candidate target polynucleotides that is specifically targeted by an RNAi trigger molecule. In other aspects, the invention provides epi-allelic series of hypomorphic RNAi trigger molecules specific for any gene of interest, where the series of RNAi trigger molecules have a variety of uses including analysis of gene function and drug target development.
摘要翻译：本发明涉及基因表达的控制。具体而言，本发明提供了生产和使用具有在体内特异性下调表达的靶基因的能力的重组核酸分子的组合物和方法。在一些方面，本发明提供了用于产生发夹DNA分子的方法，其中分子的一部分衍生自作为衍生自发夹的小干扰RNA（siRNA）的靶的mRNA。在其它方面，本发明提供用于构建产siRNA盒的合成发夹衔接寡核苷酸。在其他方面，本发明提供了用于测试RNAi触发分子的特异性抑制活性的存在或不存在的方法，并且在其它方面，本发明提供了从RNAi触发分子文库鉴定活性RNAi触发分子的方法。在其它方面，本发明提供了从由RNAi触发分子专门靶向的多个候选靶多核苷酸中鉴定多核苷酸的方法。在其它方面，本发明提供了对于任何目的基因特异性的异型RNAi触发分子的等位基因系列，其中一系列RNAi触发分子具有各种用途，包括基因功能分析和药物靶标开发。

8. 发明授权

US08334238B2 Multiparameter FACS assays to detect alterations in cellular parameters and to screen small molecule libraries 有权
标题翻译：多参数FACS测定法检测细胞参数的变化并筛选小分子文库
公开(公告)号：US08334238B2
公开(公告)日：2012-12-18
申请号：US09293670
申请日：1999-04-16
申请人： Joseph Fisher , James Lorens , Donald Payan , Alexander Rossi
发明人： Joseph Fisher , James Lorens , Donald Payan , Alexander Rossi
IPC分类号： C40B20/08 , G01N33/574
CPC分类号： G01N33/5008 , G01N15/147 , G01N33/5011 , G01N33/5014 , G01N33/502 , G01N33/5044 , G01N2015/1477 , G01N2015/149
摘要： The invention relates to novel methods of detecting alterations in cell cycle regulation in a cell or a cell population and screening for agents capable of modulating cell cycle regulation through the use of multiparameter assays and a fluorescence-activated cell sorter (FACS) machine.
摘要翻译：本发明涉及检测细胞或细胞群体中细胞周期调节变化的新方法，并且通过使用多参数测定法和荧光激活细胞分选仪（FACS）机器筛选能够调节细胞周期调节的试剂。

9. 发明申请

US20050250163A1 Modulators of angiogenesis and tumorigenesis 有权
公开(公告)号：US20050250163A1
公开(公告)日：2005-11-10
申请号：US11060659
申请日：2005-02-16
申请人： James Lorens , Sacha Holland , Weiduan Xu
发明人： James Lorens , Sacha Holland , Weiduan Xu
IPC分类号： A61K31/7105 , A61K39/395 , G01N33/50 , G01N33/53 , G01N33/574
CPC分类号： G01N33/5029 , G01N33/5011 , G01N33/5064
摘要： The present invention relates to regulation of angiogenesis and tumorigenesis. The invention further relates to methods for identifying and using agents, including small organic molecules, antibodies, peptides, cyclic peptides, nucleic acids, antisense nucleic acids, RNAi, and ribozymes, that modulate angiogenesis via modulation of endothelial cell haptotaxis; as well as to the use of expression profiles and compositions in diagnosis and therapy of angiogenesis and cancer.

10. 发明申请

US20110009281A1 METHODS FOR CREATING AND IDENTIFYING FUNCTIONAL RNA INTERFERENCE ELEMENTS 有权
标题翻译：用于创建和识别功能性RNA干扰元件的方法
公开(公告)号：US20110009281A1
公开(公告)日：2011-01-13
申请号：US12735222
申请日：2008-12-23
申请人： David Micklem , James Lorens
发明人： David Micklem , James Lorens
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04 , C40B30/04
CPC分类号： C12N15/1086 , C12N15/10 , C12N15/111 , C12N15/64 , C12N15/66 , C12N2310/14 , C12N2320/10 , C12N2330/30 , C12Q1/6897
摘要： The invention relates to the control of gene expression. Specifically, the invention provides compositions and methods for the production and use of recombinant nucleic acid molecules that have the ability to specifically downregulate an expressed target gene in vivo. In some aspects, the invention provides methods for producing a hairpin DNA molecule where part of the molecule is derived from an mRNA that is a target for a small interfering RNA (siRNA) derived from the hairpin. In other aspects, the invention provides synthetic hairpin adapter oligonucleotides that are used in the construction of siRNA-producing cassettes. In other aspects, the invention provides methods for testing for the presence or absence of specific inhibitory activity of an RNAi trigger molecule, and in still other aspects, the invention provides methods for identifying an active RNAi trigger molecule from a library of RNAi trigger molecules. In still other aspects, the invention provides methods for identifying a polynucleotide from a plurality of candidate target polynucleotides that is specifically targeted by an RNAi trigger molecule. In other aspects, the invention provides epi-allelic series of hypomorphic RNAi trigger molecules specific for any gene of interest, where the series of RNAi trigger molecules have a variety of uses including analysis of gene function and drug target development.
摘要翻译：本发明涉及基因表达的控制。具体而言，本发明提供了生产和使用具有在体内特异性下调表达的靶基因的能力的重组核酸分子的组合物和方法。在一些方面，本发明提供了用于产生发夹DNA分子的方法，其中分子的一部分衍生自作为衍生自发夹的小干扰RNA（siRNA）的靶的mRNA。在其它方面，本发明提供用于构建产siRNA盒的合成发夹衔接寡核苷酸。在其他方面，本发明提供了用于测试RNAi触发分子的特异性抑制活性的存在或不存在的方法，并且在另外的方面，本发明提供了从RNAi触发分子文库鉴定活性RNAi触发分子的方法。在其它方面，本发明提供了从由RNAi触发分子专门靶向的多个候选靶多核苷酸中鉴定多核苷酸的方法。在其它方面，本发明提供了对于任何目的基因特异性的异型RNAi触发分子的等位基因系列，其中一系列RNAi触发分子具有各种用途，包括基因功能分析和药物靶标开发。

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