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1. 发明授权

US06645766B2 Shuttle vectors 失效
公开(公告)号：US06645766B2
公开(公告)日：2003-11-11
申请号：US10043074
申请日：2002-01-07
申请人： Ying Luo , Pei Wen Yu , James Lorens
发明人： Ying Luo , Pei Wen Yu , James Lorens
IPC分类号： C12N1564
CPC分类号： C12N15/1082 , C12N15/1034 , C12N15/70 , C12N15/81
摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.

2. 发明授权

US06391582B2 Shuttle vectors 有权
标题翻译：班车向量
公开(公告)号：US06391582B2
公开(公告)日：2002-05-21
申请号：US09208827
申请日：1998-12-09
申请人： Ying Luo , Pei Wen Yu , James Lorens
发明人： Ying Luo , Pei Wen Yu , James Lorens
IPC分类号： C12P2102
CPC分类号： C12N15/1082 , C12N15/1034 , C12N15/70 , C12N15/81
摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.
摘要翻译：本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。还提供了一种方法，其中用本文提供的穿梭载体转化酵母。将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物插入载体。然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

3. 发明授权

US06280937B1 Shuttle vectors 有权
标题翻译：班车向量
公开(公告)号：US06280937B1
公开(公告)日：2001-08-28
申请号：US09133944
申请日：1998-08-14
申请人： Ying Luo , Pei Wen Yu , James Lorens
发明人： Ying Luo , Pei Wen Yu , James Lorens
IPC分类号： C12Q168
CPC分类号： C12N15/1082 , C12N15/70 , C12N15/81
摘要： The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at least yeast, and optionally, bacterial cells. Also provided is a method wherein yeast are transformed with a shuttle vector as provided herein. Heterologous nucleic acids flanked by 5′ and 3′ ends identical to a homologous recombination site within the shuttle vector are introduced to the transformed yeast and allowed to homologously recombine with the shuttle vector such that they are inserted into the vector by the yeast organism. The shuttle vector is then recovered and transferred to a mammalian cell for expression.
摘要翻译：本发明提供穿梭载体，以及使用能够在至少哺乳动物细胞中表达的穿梭载体的方法。此外，穿梭载体能够在至少酵母和任选的细菌细胞中复制。还提供了一种方法，其中用本文提供的穿梭载体转化酵母。将与穿梭载体内的同源重组位点相同的5'和3'末端的异源核酸引入转化的酵母中，并与穿梭载体同源重组，使得它们被酵母生物体插入载体中。然后将穿梭载体回收并转移至哺乳动物细胞进行表达。

4. 发明授权

US07488805B2 Apoptosis proteins 有权
标题翻译：凋亡蛋白
公开(公告)号：US07488805B2
公开(公告)日：2009-02-10
申请号：US11739267
申请日：2007-04-24
申请人： Ying Luo , Betty Huang , Mary Shen , Pei Wen Yu
发明人： Ying Luo , Betty Huang , Mary Shen , Pei Wen Yu
IPC分类号： C07K16/00
CPC分类号： C07K14/4747 , A61K38/00
摘要： The present invention is directed to novel apoptosis polypeptides such as the Apop1, Apop2, and Apop3 proteins and related molecules which are involved in modulating apoptosis and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are method for identifying novel compositions which modulate the biological activity of Apop1, Apop2, and Apop3, and the use of such compositions in diagnosis and treatment of disease.
摘要翻译：本发明涉及新的凋亡多肽，例如参与调节凋亡的Apop1，Apop2和Apop3蛋白和相关分子以及编码这些多肽的核酸分子。本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法发明。本发明还提供了鉴定调节Apop1，Apop2和Apop3的生物学活性的新组合物的方法，以及这些组合物在疾病诊断和治疗中的用途。

5. 发明授权

US07223850B1 Apoptosis proteins 有权
标题翻译：凋亡蛋白
公开(公告)号：US07223850B1
公开(公告)日：2007-05-29
申请号：US09762491
申请日：1999-08-06
申请人： Ying Luo , Betty Huang , Mary Shen , Pei Wen Yu
发明人： Ying Luo , Betty Huang , Mary Shen , Pei Wen Yu
IPC分类号： C12N15/11 , C07K14/00
CPC分类号： C12N9/12
摘要： The present invention is directed to novel apoptosis polypeptides such as the Apop1, and Apop3 proteins and related molecules which are involved in modulating apoptosis and to nucleic acid molecules encoding those polypeptides. Also provided here are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided by the present invention are methods for identifying novel compositions which modulate the biological activity of Apop1, Apop2, and Apop3, and the use of such compositions in diagnosis and treatment of disease.
摘要翻译：本发明涉及新的凋亡多肽，例如涉及调节凋亡的Apop1和Apop3蛋白和相关分子以及编码那些多肽的核酸分子。此处还提供了包含这些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法发明。本发明进一步提供了用于鉴定调节Apop1，Apop2和Apop3的生物活性的新组合物的方法，以及这些组合物在诊断和治疗疾病中的用途。

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