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1. 发明授权

US09771401B2 Green fluorescent protein (GFP) peptides from Rhacostoma 有权
公开(公告)号：US09771401B2
公开(公告)日：2017-09-26
申请号：US14777311
申请日：2014-03-17
申请人： Michael R. Tota , William W. Ward
发明人： Michael R. Tota , William W. Ward
IPC分类号： C07K14/435 , G01N33/58
CPC分类号： C07K14/43595 , C07K2319/60 , G01N33/582 , G01N2333/43595
摘要： The present technology is directed to the nucleic acid molecule encoding novel fluorescent proteins, in particular, green fluorescent proteins (GFPs), such as those that may be isolated from an organism of genus Rhacostoma, as well as compositions comprising the same and methods for analyzing a physiologically active substance in a cell wherein the fluorescent proteins are expressed in the cell.

2. 发明授权

US09075058B2 Coelenterazine analogues and coelenteramide analogues 有权
公开(公告)号：US09075058B2
公开(公告)日：2015-07-07
申请号：US14609539
申请日：2015-01-30
申请人： JNC Corporation , National University Corporation Tokyo Medical and Dental University
发明人： Satoshi Inouye , Yuiko Sahara , Takamitsu Hosoya
IPC分类号： C12N9/96 , G01N33/53 , C12Q1/66 , C12Q1/68 , C07D241/12 , G01N33/573 , C07D241/20 , C07D409/04 , C07D495/04 , C07D495/14 , C07D409/14 , G01N21/64
CPC分类号： G01N33/573 , C07D241/12 , C07D241/20 , C07D409/04 , C07D409/14 , C07D487/04 , C07D495/04 , C07D495/14 , C12N9/96 , C12Q1/66 , C12Q1/68 , G01N21/6428 , G01N33/53 , G01N2333/43595 , G01N2333/90241
摘要： Coelenterazine analogs with different luminescence properties from conventional ones and coelenteramide analogs with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogs modified at the 8-position of coelenterazine and coelenteramide analogs modified at the 2- or 3-position of coelenteramide.

3. 发明申请

US20140316137A1 COELENTERAZINE ANALOGUES AND COELENTERAMIDE ANALOGUES 审中-公开
标题翻译：阿司匹林类似物和烯丙胺类似物
公开(公告)号：US20140316137A1
公开(公告)日：2014-10-23
申请号：US14324962
申请日：2014-07-07
申请人： JNC Corporation , National University Corporation Tokyo Medical and Dental University
发明人： Satoshi Inouye , Yuiko Sahara , Takamitsu Hosoya
IPC分类号： C07D495/14 , C07D409/14 , C07D495/04 , C07D241/20 , C07D409/04
CPC分类号： G01N33/573 , C07D241/12 , C07D241/20 , C07D409/04 , C07D409/14 , C07D487/04 , C07D495/04 , C07D495/14 , C12N9/96 , C12Q1/66 , C12Q1/68 , G01N21/6428 , G01N33/53 , G01N2333/43595 , G01N2333/90241
摘要： Coelenterazine analogs with different luminescence properties from conventional ones and coelenteramide analogs with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogs modified at the 8-position of coelenterazine and coelenteramide analogs modified at the 2- or 3-position of coelenteramide.
摘要翻译：已经期望具有不同于常规荧光发光性质的Coelenterazine类似物和具有不同于常规荧光性质的coelenteramide类似物。本发明提供在coelenterazine的8位修饰的肠溶素类似物和在肠腔酰胺的2-或3-位修饰的coelenteramide类似物。

4. 发明申请

US20140120538A1 COELENTERAZINE ANALOGUES AND COELENTERAMIDE ANALOGUES 有权
标题翻译：阿司匹林类似物和烯丙胺类似物
公开(公告)号：US20140120538A1
公开(公告)日：2014-05-01
申请号：US14095421
申请日：2013-12-03
申请人： National University Corporation Tokyo Medical and Dental University , JNC Corporation
发明人： SATOSHI INOUYE , YUIKO SAHARA , TAKAMITSU HOSOYA
IPC分类号： C12N9/96 , G01N33/53 , C12Q1/66 , C07D409/14 , C07D409/04 , C07D495/04 , C07D495/14 , C12Q1/68 , C07D241/12
CPC分类号： G01N33/573 , C07D241/12 , C07D241/20 , C07D409/04 , C07D409/14 , C07D487/04 , C07D495/04 , C07D495/14 , C12N9/96 , C12Q1/66 , C12Q1/68 , G01N21/6428 , G01N33/53 , G01N2333/43595 , G01N2333/90241
摘要： Coelenterazine analogues with different luminescence properties from conventional ones and coelenteramide analogues with different fluorescence properties from conventional ones have been desired. The invention provides coelenterazine analogues modified at the 8-position of coelenterazine and coelenteramide analogues modified at the 2- or 3-position of coelenteramide.
摘要翻译：已经期望具有不同于常规荧光发光性质的Coelenterazine类似物和具有不同于常规荧光性质的coelenteramide类似物。本发明提供在coelenterazine和coelenteramide类似物的8位修饰的coelenterazine类似物，其在coelenteramide的2-或3-位修饰。

5. 发明授权

US08420327B2 Analyte sensors, methods for preparing and using such sensors, and methods of detecting analyte activity 有权
标题翻译：分析物传感器，制备和使用这些传感器的方法以及检测分析物活性的方法
公开(公告)号：US08420327B2
公开(公告)日：2013-04-16
申请号：US12448101
申请日：2007-12-14
申请人： April L. Ellis , Yiming Ye , Angela Holder , Yun Huang , Michael Kirberger , Jenny Jie Yang , Jin Zou , Wei Yang
发明人： April L. Ellis , Yiming Ye , Angela Holder , Yun Huang , Michael Kirberger , Jenny Jie Yang , Jin Zou , Wei Yang
IPC分类号： G01N33/53
CPC分类号： G01N21/6486 , G01N33/542 , G01N33/84 , G01N2333/43595
摘要： Embodiments of the present disclosure provide for analyte sensors, methods for producing and using the analyte sensors, methods of detecting and/or measuring analyte activity, methods for characterizing analyte cellular activity, methods of detecting pH change in a system, method of controlling the concentration of an analyte in a system, fusion proteins, polynucleotides, and vectors corresponding to the analyte sensors, kits, and the like.
摘要翻译：本公开的实施方案提供了分析物传感器，用于生产和使用分析物传感器的方法，检测和/或测量分析物活性的方法，用于表征分析物细胞活性的方法，检测系统中pH变化的方法，控制浓度的方法的系统中的分析物，融合蛋白，多核苷酸和对应于分析物传感器，试剂盒等的载体。

6. 发明授权

US07853411B2 System for cell-based screening 有权
标题翻译：用于细胞筛选的系统
公开(公告)号：US07853411B2
公开(公告)日：2010-12-14
申请号：US10685737
申请日：2003-10-15
申请人： Richard Rubin , Kenneth Giuliano , Albert Gough , R. Terry Dunlay , Bruce Ray Conway
发明人： Richard Rubin , Kenneth Giuliano , Albert Gough , R. Terry Dunlay , Bruce Ray Conway
IPC分类号： G01N33/52 , G06F19/00
CPC分类号： G01N33/582 , B01J2219/00315 , B01J2219/00317 , B01J2219/00707 , B01J2219/00743 , B01L3/5085 , B82Y5/00 , B82Y30/00 , C12Q1/68 , C12Q1/6813 , C40B60/14 , G01N15/1475 , G01N21/6428 , G01N21/6452 , G01N33/5008 , G01N33/5014 , G01N33/5035 , G01N33/5076 , G01N33/5079 , G01N33/533 , G01N33/566 , G01N2015/1472 , G01N2015/1479 , G01N2015/1486 , G01N2015/1488 , G01N2015/1497 , G01N2035/00158 , G01N2035/0424 , G01N2333/43595 , G01N2333/726 , G06K9/0014 , C12Q2525/179
摘要： The present invention provides systems, methods, and screens to measure receptor internalization in a single step with appropriate automation and throughput. This approach involves luminescent labeling of the receptor of interest and the automated measurement of receptor internalization to a perinuclear location.
摘要翻译：本发明提供用于在单个步骤中测量受体内化的系统，方法和屏幕，具有适当的自动化和生产量。该方法涉及感兴趣受体的发光标记和受体内化至核周位置的自动测量。

7. 发明申请

US20070254373A1 Fragments of fluorescent proteins for protein fragment complementation assays 有权
标题翻译：用于蛋白质片段互补测定的荧光蛋白片段
公开(公告)号：US20070254373A1
公开(公告)日：2007-11-01
申请号：US11656543
申请日：2007-01-23
申请人： Stephen Michnick , Marnie MacDonald , Jane Lamerdin
发明人： Stephen Michnick , Marnie MacDonald , Jane Lamerdin
IPC分类号： G01N33/00 , C07K14/00
CPC分类号： C07K14/43595 , G01N33/542 , G01N33/58 , G01N33/582 , G01N33/68 , G01N33/6845 , G01N2333/43595
摘要： The present invention is directed to Protein-fragment Complementation Assays (PCAs) and assay compositions based on fluorescent proteins. The invention provides methods for fragmenting fluorescent proteins and generating mutant fragments with desired spectral characteristics for PCA. The invention encompasses assays and compositions based on fluorescent proteins from the species Aequorea, Anemonia and Anthozoa. In particular, the invention is directed to fragments of mutant fluorescent proteins having improved spectral properties over the wild-type proteins. The invention encompasses fragments of mutant versions of A. Victoria green fluorescent protein (GFP), in particular yellow fluorescent proteins (EYFP and super-EYFP), ‘Venus’, cyan, ‘citrine’, blue, cyan-green, and photoactivatable variants of GFP The invention also encompasses red fluorescent PCAs based on Discosoma red fluorescent protein (RFP PCA)and a kindling fluorescent protein PCA (KFP1 PCA) derived from Anemonia sulcata. Any useful mutation of a fluorescent protein can be engineered into a fragment, generating a wide range of assays useful for drug discovery, target validation, high-throughput screening, high-content screening, pathway mapping, drug mechanism-of-action studies, biosensors, and diagnostics.
摘要翻译：本发明涉及基于荧光蛋白的蛋白质片段互补测定（PCA）和测定组合物。本发明提供了用于分解荧光蛋白并产生具有PCA所需光谱特征的突变片段的方法。本发明包括基于来自Aequorea，Anemonia和Anthozoa的荧光蛋白的测定和组合物。特别地，本发明涉及具有比野生型蛋白质更好的光谱性质的突变荧光蛋白的片段。本发明包括A.维多利亚绿色荧光蛋白（GFP），特别是黄色荧光蛋白（EYFP和超EYFP），“维纳斯”，青色“黄水晶”，蓝色，青绿色和可光活化变体的突变体版本的片段的GFP本发明还包括基于Discosoma红色荧光蛋白（RFP PCA）的红色荧光PCA和源自Anemonia sulcata的点燃荧光蛋白PCA（KFP1 PCA）。荧光蛋白的任何有用的突变可被工程化成片段，产生广泛的用于药物发现，靶标验证，高通量筛选，高含量筛选，途径作图，药物作用机理研究，生物传感器，和诊断。

8. 发明申请

US20050123909A1 COMPOSITIONS AND METHODS FOR TREATING CELLS HAVING DOUBLE MINUTE DNA 失效
标题翻译：用于治疗具有双分子DNA的细胞的组合物和方法
公开(公告)号：US20050123909A1
公开(公告)日：2005-06-09
申请号：US09229229
申请日：1999-01-12
申请人： GEOFFREY M. WAHL , NORIAKI SHIMIZU , TERU KANDA , H. MICHAEL SHEPARD
发明人： GEOFFREY M. WAHL , NORIAKI SHIMIZU , TERU KANDA , H. MICHAEL SHEPARD
IPC分类号： A61K45/00 , A61K48/00 , A61K49/00 , A61P35/00 , A61P43/00 , C07K14/00 , C12N15/09 , C12Q1/02 , C12Q1/68 , G01N33/15 , G01N33/50 , G01N33/52 , G01N33/574 , G01N33/58 , G01N33/68
CPC分类号： C12Q1/68 , G01N33/5011 , G01N33/6875 , G01N2333/43595
摘要： This invention provides methods by which test substances can be screened for their ability to inhibit, enhance or eliminate double minute (DM) or extrachromosomal DNA by micronucleation in cells. This invention also provides a method for inducing maturation or death of a cell having the capacity to generate micronuclei. It also provides a method of treating a disease in a subject, the cells correlated with the disease having DM and extrachromosomal DNA as well as the capacity to generate micronuclei to capture them. Further provided is a method of detecting chromosomal and extrachromosomal DNA in a cell.
摘要翻译：本发明提供了通过细胞中微核来筛选测试物质以抑制，增强或消除双分钟（DM）或染色体外DNA的能力的方法。本发明还提供了诱导具有产生微核能力的细胞的成熟或死亡的方法。它还提供了治疗受试者疾病的方法，与具有DM和染色体外DNA的疾病相关的细胞以及产生微核以捕获它们的能力。还提供了检测细胞中染色体和染色体外DNA的方法。

9. 发明授权

US06803188B1 Tandem fluorescent protein constructs 失效
标题翻译：串联荧光蛋白构建体
公开(公告)号：US06803188B1
公开(公告)日：2004-10-12
申请号：US08594575
申请日：1996-01-31
申请人： Roger Y. Tsien , Roger Heim
发明人： Roger Y. Tsien , Roger Heim
IPC分类号： C07K1400
CPC分类号： C12Q1/485 , C07K14/43595 , C07K2319/00 , C12Q1/37 , G01N33/542 , G01N2333/43595
摘要： This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.
摘要翻译：本发明提供了包括供体荧光蛋白部分，受体荧光蛋白部分和连接供体和受体部分的接头部分的串联荧光蛋白构建体。供体和受体部分表现出在切割时消除的荧光共振能量转移。所述构建体可用于酶测定。

10. 发明申请

US20030104490A1 Assay 审中-公开
公开(公告)号：US20030104490A1
公开(公告)日：2003-06-05
申请号：US10311409
申请日：2002-12-16
发明人： Laura Margaret Fletcher , Jeremy Myles Tavare
IPC分类号： G01N033/53 , G01N033/567 , C07H021/04 , C07K014/65
CPC分类号： G01N33/502 , C07K14/54 , C07K2319/00 , G01N33/5008 , G01N2333/43595 , G01N2333/62
摘要： There is disclosed a method of determining whether a candidate compound mimics or antagonizes effects of insulin, comprising: (a) providing a transfected host cell comprising a nucleic acid sequence which encodes a protein construct comprising an IRAP protein moiety and a detectable protein moiety, wherein the detectable protein moiety is fused to a luminal domain of the IRAP protein moiety and further wherein the detectable protein moiety is capable of being assayed by a first detection method capable of detecting the protein moiety when located exofacially but not when located intracellularly and a second detection method capable of detecting the protein moiety both when located exofacially and when located intracellularly; (b) contacting the transfected host cell with a candidate compound; (c) measuring the amount of the exofacial protein moiety by the said first detection method and measuring the total amount of exofacial and intracellular detectable protein moiety by the said second detection method; and (d) comparing the amount of exofacial detectable protein moiety with the total amount of exofacial and intracellular detectable protein moiety to provide a measure of the extent of GLUT4 vesicle translocation. In an alternative method, the protein construct comprises an IRAP protein moiety, a first detectable protein moiety which is fused to a luminal domain of the IRAP protein moiety and a second detectable protein moiety which is fused to a cytoplasmic domain of the IRAP protein moiety, wherein the first detectable protein moiety is capable of being assayed by a first detection method capable of detecting the first protein moiety when located exofacially but not when located intracellularly and wherein the second detectable protein moiety is capable of being assayed by a second detection method capable of detecting the second protein moiety when located intracellularly. The assays of the invention may be used to determine whether a nullcandidate compoundnull is a mimic or antagonist of insulin. The present invention may be used to test libraries of compounds in an automated high throughput screen.

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