专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20100137160A1 Method and Device for Analyzing Biomolecules With Track - Etched Polymeric Layers 有权
标题翻译：用跟踪蚀刻聚合物层分析生物分子的方法和装置
公开(公告)号：US20100137160A1
公开(公告)日：2010-06-03
申请号：US12626405
申请日：2009-11-25
申请人： Vladimir KNEZEVIC , Dan-Paul Hartmann , Michael R. Emmert-Buck
发明人： Vladimir KNEZEVIC , Dan-Paul Hartmann , Michael R. Emmert-Buck
IPC分类号： C40B30/04 , C40B60/12
CPC分类号： G01N33/6845 , B01J19/0046 , B01J2219/00416 , B01J2219/00533 , B01J2219/00574 , B01J2219/00576 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00668 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01L3/50255 , B01L2300/0627 , B01L2300/0829 , B01L2400/049 , C40B40/06 , C40B40/10 , G01N33/54373 , G01N2500/00
摘要： The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
摘要翻译：本公开提供了允许在源自多样品保持器（例如多孔板）的样品中同时检测大量目标生物分子的方法，装置和试剂盒。一个具体的示例方法是制备多孔样品保持器的生物分子含量的多个实质性复制品的方法。还提供了用于执行所述方法的设备和套件。

2. 发明授权

US07838222B2 Methods, devices and kits for multiplex blotting of biological samples from multi-well plates 有权
标题翻译：用于从多孔板多次印迹生物样品的方法，装置和试剂盒
公开(公告)号：US07838222B2
公开(公告)日：2010-11-23
申请号：US11277227
申请日：2006-03-22
申请人： Vladimir Knezevic , Dan-Paul Hartmann , Michael R. Emmert-Buck
发明人： Vladimir Knezevic , Dan-Paul Hartmann , Michael R. Emmert-Buck
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/6845 , B01J19/0046 , B01J2219/00416 , B01J2219/00533 , B01J2219/00574 , B01J2219/00576 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00668 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01L3/50255 , B01L2300/0627 , B01L2300/0829 , B01L2400/049 , C40B40/06 , C40B40/10 , G01N33/54373 , G01N2500/00
摘要： The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
摘要翻译：本公开提供了允许在源自多样品保持器（例如多孔板）的样品中同时检测大量目标生物分子的方法，装置和试剂盒。一个具体的示例方法是制备多孔样品保持器的生物分子含量的多个实质性复制品的方法。还提供了用于执行所述方法的设备和套件。

3. 发明授权

US08779312B2 Method and device for analyzing biomolecules with track-etched polymeric layers 有权
标题翻译：用轨道蚀刻聚合物层分析生物分子的方法和装置
公开(公告)号：US08779312B2
公开(公告)日：2014-07-15
申请号：US12626405
申请日：2009-11-25
申请人： Vladimir Knezevic , Dan-Paul Hartmann , Michael R. Emmert-Buck
发明人： Vladimir Knezevic , Dan-Paul Hartmann , Michael R. Emmert-Buck
IPC分类号： H01H1/40 , C12Q1/68
CPC分类号： G01N33/6845 , B01J19/0046 , B01J2219/00416 , B01J2219/00533 , B01J2219/00574 , B01J2219/00576 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00668 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01L3/50255 , B01L2300/0627 , B01L2300/0829 , B01L2400/049 , C40B40/06 , C40B40/10 , G01N33/54373 , G01N2500/00
摘要： The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
摘要翻译：本公开提供了允许在源自多样品保持器（例如多孔板）的样品中同时检测大量目标生物分子的方法，装置和试剂盒。一个具体的示例方法是制备多孔样品保持器的生物分子含量的多个实质性复制品的方法。还提供了用于执行所述方法的设备和套件。

4. 发明授权

US06969615B2 Methods, devices, arrays and kits for detecting and analyzing biomolecules 有权
标题翻译：用于检测和分析生物分子的方法，装置，阵列和试剂盒
公开(公告)号：US06969615B2
公开(公告)日：2005-11-29
申请号：US10432423
申请日：2001-11-20
申请人： Vladimir Knezevic , Michael R. Emmert-Buck , Galina Baibakova , Dan-Paul Hartmann , Stephen M. Hewitt , Capre Denise Mitchell , Kevin Gardner
发明人： Vladimir Knezevic , Michael R. Emmert-Buck , Galina Baibakova , Dan-Paul Hartmann , Stephen M. Hewitt , Capre Denise Mitchell , Kevin Gardner
IPC分类号： B01L3/00 , G01N1/28 , G01N33/543
CPC分类号： B01L3/5023 , G01N1/2813 , G01N27/44739 , G01N33/54386 , G01N2001/284 , G01N2001/368
摘要： The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating “carbon copies” of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e.g., proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and/or tissue, especially human cells and/or tissue. The disclosure also provides a plurality of differentially prepared and/or processed membranes that can be used in described methods, and which permit the identification and analysis of biomolecules.
摘要翻译：本公开涉及用于检测组织部分（例如新鲜或归档样品，组织微阵列或通过LCM程序收获的细胞）中的生物分子或其他基本上二维样品（例如，通过产生从样品洗脱的生物分子的“碳拷贝”并使用对感兴趣的生物分子具有特异性亲和力的一个或多个检测器分子（例如，抗体或DNA探针）使拷贝上的生物分子可视化，从而形成电泳凝胶或cDNA微阵列。提供了用于鉴定样品中生物分子（例如蛋白质和核酸）的模式的具体方法。提供了其他具体方法用于鉴定和分析由细胞和/或组织，特别是人细胞和/或组织产生的蛋白质和其它生物分子。本公开还提供可以在所述方法中使用并且允许生物分子的鉴定和分析的多种差异制备和/或加工的膜。

5. 发明授权

US06602661B1 Methods and arrays for detecting biomolecules 有权
标题翻译：检测生物分子的方法和阵列
公开(公告)号：US06602661B1
公开(公告)日：2003-08-05
申请号：US09718990
申请日：2000-11-20
申请人： Vladimir Knezevic , Michael R. Emmert-Buck
发明人： Vladimir Knezevic , Michael R. Emmert-Buck
IPC分类号： G01N3353
CPC分类号： G01N33/6803 , C12Q1/6841 , C40B30/04 , G01N33/54386 , G01N33/57434 , G01N33/6845 , G01N2333/4712 , G01N2333/4742 , G01N2333/7051 , G01N2333/96455 , G01N2333/96486
摘要： The present invention is directed to a device and a method for detecting biomolecules in a tissue section or other two-dimensional sample by creating “carbon copies” of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using antibodies or DNA probes having specific affinity for the biomolecules of interest. Thin membranes in a stacked or layered configuration are applied to the sample, such as a tissue section, and reagents and reaction conditions are provided so that the biomolecules are eluted from the sample and transferred onto each of the stacked membranes thereby producing multiple replicas of the biomolecular content of the sample.
摘要翻译：本发明涉及一种用于通过从样品中洗脱的生物分子的“碳拷贝”来检测组织切片或其它二维样品中的生物分子的装置和方法，并使用具有对感兴趣的生物分子的特异性亲和力。叠层或分层结构的薄膜被施加到样品例如组织切片上，并提供试剂和反应条件，使得生物分子从样品中洗脱并转移到每个层叠的膜上，从而产生多个复制品样品的生物分子内容物。

6. 发明申请

US20140066752A1 Methods and Systems for Using Therapeutic, Diagnostic or Prophylactic Magnetic Agents 有权
标题翻译：使用治疗，诊断或预防性磁性药物的方法和系统
公开(公告)号：US20140066752A1
公开(公告)日：2014-03-06
申请号：US14075408
申请日：2013-11-08
申请人： Michael R. Emmert-Buck , Benjamin Shapiro
发明人： Benjamin Shapiro , Michael R. Emmert-Buck
IPC分类号： A61N2/00 , A61B5/055
CPC分类号： A61N2/00 , A61B5/055 , A61N1/406 , A61N2/02 , A61N2/06 , B33Y80/00
摘要： Systems and methods are disclosed for directing magnetizable particles comprising therapeutic agents to a target volume, or for guiding magnetizable particles comprising therapeutic agents from a first target volume to a second target volume, at a distance using a magnetic field, to enable the treatment of diseased areas including areas deep inside a patient's body. The methods may be used to diagnose or treat diseased areas within a patient, for example tumors of the lungs, intestines, and liver, and is also useful in enhancing the permeability of solid tumors to chemotherapeutic agents.
摘要翻译：公开了用于将包含治疗剂的可磁化颗粒引导至靶体积的系统和方法，或者使用磁场以一定距离将包含治疗剂的可磁化颗粒从第一目标体积引导至第二靶体积，以使患者能够治疗包括患者身体深处的区域。该方法可用于诊断或治疗患者内的患病区域，例如肺，肠和肝脏的肿瘤，并且还可用于增强实体瘤对化疗剂的渗透性。

7. 发明授权

US07214477B1 Layered device with capture regions for cellular analysis 有权
标题翻译：具有捕获区域的分层设备进行细胞分析
公开(公告)号：US07214477B1
公开(公告)日：2007-05-08
申请号：US10048194
申请日：2000-07-26
申请人： Michael R. Emmert-Buck
发明人： Michael R. Emmert-Buck
IPC分类号： C12Q1/00
CPC分类号： G01N33/54386
摘要： The present invention involves methods, systems, and devices for analyzing a biological material, such as a cellular or other specimen. The method includes placing the specimen on a substrate having different capture regions, such as contiguous layers, wherein the different capture regions of the substrate contain different identification molecules, and transferring components of the specimen through the capture regions under conditions that allow the components to interact with different identification molecules in the different regions of the substrate. The components of the specimen can be transferred through the different layers (or other regions) of the substrate by capillary action of a solution moving through the cellular specimen or by electrophoresis. The transfer of components of the specimen through the substrate may occur while maintaining a geometric correspondence to the specimen, such as the cytoarchitecture of a cellular specimen, for example by moving the components through parallel layers having positions that correspond to positions within the specimen. When the cellular architecture of the specimen is maintained, a correlation between the different identification molecules and the components of the cellular specimens may be made. The analysis can occur with one or more different discrete (for example cellular) specimens on a surface of the substrate. Examples of cellular specimens include, but are not limited to tissue sections, particularly tumor tissue sections. The cellular specimen can also include cultured cells or a cytology sample. Cytostat tissue sections cut slightly thicker than usual, that is about 25 to about 50 μm, improves the ability to detect molecules of moderate and low level abundance.
摘要翻译：本发明涉及用于分析生物材料（例如细胞或其他样品）的方法，系统和装置。该方法包括将样品放置在具有不同捕获区域（例如连续层）的基底上，其中衬底的不同捕获区域含有不同的识别分子，并且在允许组分相互作用的条件下将样品的组分转移通过捕获区域在基质的不同区域具有不同的识别分子。样品的组分可以通过移动通过细胞样品的溶液的毛细管作用或通过电泳转移通过衬底的不同层（或其他区域）。可以在保持与样品几何对应的情况下，例如细胞样品的细胞架构，例如通过将具有与样品中的位置相对应的位置的平行层移动组件，可以发生样品的通过基底的成分的转移。当维持样品的细胞结构时，可以制备不同识别分子与细胞样品组分之间的相关性。分析可以在衬底的表面上的一个或多个不同的离散（例如细胞）样品发生。细胞样品的实例包括但不限于组织切片，特别是肿瘤组织切片。细胞标本还可以包括培养的细胞或细胞学样品。细胞定型组织切片比通常切割的厚度大约为25至50微米，提高检测中等和低水平丰度的分子的能力。

8. 发明授权

US08283158B2 Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two dimensional array 失效
标题翻译：用于在二维阵列中执行多个同时操作生物分子的方法和装置
公开(公告)号：US08283158B2
公开(公告)日：2012-10-09
申请号：US12587976
申请日：2009-10-14
申请人： Michael R. Emmert-Buck , Rodrigo F. Chuaqui , Michael A. Tangrea
发明人： Michael R. Emmert-Buck , Rodrigo F. Chuaqui , Michael A. Tangrea
IPC分类号： C12M1/34
CPC分类号： C12Q1/6837 , B01J2219/00641 , B01J2219/00722 , B01J2219/00725 , C12Q1/6806
摘要： This invention relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols.
摘要翻译：本发明涉及在二维阵列（例如凝胶，膜，组织活组织检查等）中进行多重同时操作生物分子的方法和装置。这种操作特别包括测定和核酸扩增方案。

9. 发明授权

US07226731B1 PB 39, a gene dysregulated in prostate cancer, and uses thereof 失效
标题翻译： PB39，前列腺癌失调的基因及其用途
公开(公告)号：US07226731B1
公开(公告)日：2007-06-05
申请号：US09743825
申请日：1999-07-23
申请人： Rodrigo F. Chuaqui , Kristina A. Cole , Lance A. Liotta , Michael R. Emmert-Buck
发明人： Rodrigo F. Chuaqui , Kristina A. Cole , Lance A. Liotta , Michael R. Emmert-Buck
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/158
摘要： A novel gene, PB39, that is up-regulated, or over-expressed, in prostate cancer has been identified. The gene has been identified by means of its cDNA obtained by reverse transcription of the corresponding mRNA. Microdissection of prostate glands that had been surgically removed from prostate cancer patients revealed a novel up-regulated transcript in an aggressive prostate carcinoma. Differential analysis for the presence of this gene was carried out from the same glands by comparing transcription in microdissected normal prostatic epithelium versus that in microdissected invasive tumor. The transcript was over-expressed in 5 of 10 prostate carcinomas examined. A variant transcript was over-expressed in 4 of 4 prostate carcinomas, and was found in 1 of 4 normal samples. The invention provides a purified and isolated nucleic acid that includes the sequence of PB39 or its complement, the sequence of a variant of PB39 or its complement, and a primer or probe, that includes a sequence that is a fragment of these sequences. Additionally, the polypeptide encoded by these genes, an antibody to the polypeptide, and methods of detection of PB39 or its gene product are provided.
摘要翻译：已经鉴定出在前列腺癌中上调或过度表达的新基因PB39。该基因已通过其相应mRNA的逆转录获得的cDNA鉴定。从前列腺癌患者手术切除的前列腺的显微解剖显示在侵袭性前列腺癌中有一种新的上调的转录物。通过比较显微解剖正常前列腺上皮中的转录与微切割侵袭性肿瘤中的转录，从相同腺体进行差异分析。在检查的10个前列腺癌中的5个中，转录物过表达。在4个前列腺癌中的4个中，变体转录物过表达，并且在4个正常样品中的1个中发现。本发明提供了纯化和分离的核酸，其包括PB39或其互补序列，PB39或其互补序列变体的序列，以及引物或探针，其包括作为这些序列的片段的序列。另外，提供了由这些基因编码的多肽，多肽的抗体，以及检测PB39或其基因产物的方法。

10. 发明授权

US08597715B2 Target activated microtransfer 有权
标题翻译：靶活化微转移
公开(公告)号：US08597715B2
公开(公告)日：2013-12-03
申请号：US12753566
申请日：2010-04-02
申请人： Michael R. Emmert-Buck , Michael Anthony Tangrea , Robert F. Bonner , Rodrigo Chuaqui , Thomas J. Pohida
发明人： Michael R. Emmert-Buck , Michael Anthony Tangrea , Robert F. Bonner , Rodrigo Chuaqui , Thomas J. Pohida
IPC分类号： B05D1/00
CPC分类号： G01N33/56966 , G01N33/543 , Y10T428/2813 , Y10T428/2839
摘要： A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly (for example with a heat generating enzyme carried by the immunoreagent), or indirectly (for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorophore that absorbs light and emits heat.
摘要翻译：一种从生物样品中除去靶的方法，其包括将转移表面与生物样品接触，然后焦点改变转移表面以允许靶与生物样品的选择性分离。在所公开的实施方案中，靶是组织切片的细胞或细胞组分，并且转移表面是可以被焦点改变以将靶附着到转移表面的膜。随后从组织切片分离膜，从组织切片选择性地去除粘附的靶。转移表面从靶内活化，以将靶附着到转印表面，例如通过加热靶以将其粘附到热塑性转印表面。可以通过将生物样品暴露于特异性结合靶（或靶的成分）的免疫反应物来实现这种原位激活。免疫反应物可以直接（例如用免疫反应物携带的发热酶）或间接地（例如通过改变靶的特征）来改变转移表面。在一些实施方案中，免疫反应物在靶中沉积沉淀物，其相对于周围组织增加其光吸收，使得生物样品可暴露于光以选择性加热靶标。或者，免疫反应剂是携带吸收光并发出热量的荧光团的免疫荧光剂。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式