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1. 发明授权

US07226731B1 PB 39, a gene dysregulated in prostate cancer, and uses thereof 失效
标题翻译： PB39，前列腺癌失调的基因及其用途
公开(公告)号：US07226731B1
公开(公告)日：2007-06-05
申请号：US09743825
申请日：1999-07-23
申请人： Rodrigo F. Chuaqui , Kristina A. Cole , Lance A. Liotta , Michael R. Emmert-Buck
发明人： Rodrigo F. Chuaqui , Kristina A. Cole , Lance A. Liotta , Michael R. Emmert-Buck
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/158
摘要： A novel gene, PB39, that is up-regulated, or over-expressed, in prostate cancer has been identified. The gene has been identified by means of its cDNA obtained by reverse transcription of the corresponding mRNA. Microdissection of prostate glands that had been surgically removed from prostate cancer patients revealed a novel up-regulated transcript in an aggressive prostate carcinoma. Differential analysis for the presence of this gene was carried out from the same glands by comparing transcription in microdissected normal prostatic epithelium versus that in microdissected invasive tumor. The transcript was over-expressed in 5 of 10 prostate carcinomas examined. A variant transcript was over-expressed in 4 of 4 prostate carcinomas, and was found in 1 of 4 normal samples. The invention provides a purified and isolated nucleic acid that includes the sequence of PB39 or its complement, the sequence of a variant of PB39 or its complement, and a primer or probe, that includes a sequence that is a fragment of these sequences. Additionally, the polypeptide encoded by these genes, an antibody to the polypeptide, and methods of detection of PB39 or its gene product are provided.
摘要翻译：已经鉴定出在前列腺癌中上调或过度表达的新基因PB39。该基因已通过其相应mRNA的逆转录获得的cDNA鉴定。从前列腺癌患者手术切除的前列腺的显微解剖显示在侵袭性前列腺癌中有一种新的上调的转录物。通过比较显微解剖正常前列腺上皮中的转录与微切割侵袭性肿瘤中的转录，从相同腺体进行差异分析。在检查的10个前列腺癌中的5个中，转录物过表达。在4个前列腺癌中的4个中，变体转录物过表达，并且在4个正常样品中的1个中发现。本发明提供了纯化和分离的核酸，其包括PB39或其互补序列，PB39或其互补序列变体的序列，以及引物或探针，其包括作为这些序列的片段的序列。另外，提供了由这些基因编码的多肽，多肽的抗体，以及检测PB39或其基因产物的方法。

2. 发明授权

US08283158B2 Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two dimensional array 失效
标题翻译：用于在二维阵列中执行多个同时操作生物分子的方法和装置
公开(公告)号：US08283158B2
公开(公告)日：2012-10-09
申请号：US12587976
申请日：2009-10-14
申请人： Michael R. Emmert-Buck , Rodrigo F. Chuaqui , Michael A. Tangrea
发明人： Michael R. Emmert-Buck , Rodrigo F. Chuaqui , Michael A. Tangrea
IPC分类号： C12M1/34
CPC分类号： C12Q1/6837 , B01J2219/00641 , B01J2219/00722 , B01J2219/00725 , C12Q1/6806
摘要： This invention relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols.
摘要翻译：本发明涉及在二维阵列（例如凝胶，膜，组织活组织检查等）中进行多重同时操作生物分子的方法和装置。这种操作特别包括测定和核酸扩增方案。

3. 发明申请

US20100216166A1 TARGET ACTIVATED MICROTRANSFER 有权
标题翻译：目标活化微量RNA
公开(公告)号：US20100216166A1
公开(公告)日：2010-08-26
申请号：US12713105
申请日：2010-02-25
申请人： Robert F. Bonner , Thomas J. Pohida , Michael R. Emmert-Buck , Michael Anthony Tangrea , Rodrigo F. Chuaqui
发明人： Robert F. Bonner , Thomas J. Pohida , Michael R. Emmert-Buck , Michael Anthony Tangrea , Rodrigo F. Chuaqui
IPC分类号： G01N33/567
CPC分类号： G01N33/543 , G01N33/56966 , G01N2001/284 , Y10T428/2813
摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.
摘要翻译：一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及在照射期间向转印膜施加恒定压力的装置。

4. 发明申请

US20100105056A1 Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two dimentional array 失效
标题翻译：用于在两维阵列中执行多次同时操作生物分子的方法和装置
公开(公告)号：US20100105056A1
公开(公告)日：2010-04-29
申请号：US12587976
申请日：2009-10-14
申请人： Michael R. Emmert-Buck , Rodrigo F. Chuaqui , Michael A. Tangrea
发明人： Michael R. Emmert-Buck , Rodrigo F. Chuaqui , Michael A. Tangrea
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J2219/00641 , B01J2219/00722 , B01J2219/00725 , C12Q1/6806
摘要： This invention relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols.
摘要翻译：本发明涉及在二维阵列（诸如凝胶，膜，组织活组织检查等）中进行多重同时操作生物分子的方法和装置。这种操作特别包括测定和核酸扩增方案。

5. 发明授权

US07695752B2 Target activated microtransfer 有权
标题翻译：靶活化微转移
公开(公告)号：US07695752B2
公开(公告)日：2010-04-13
申请号：US11202848
申请日：2005-08-12
申请人： Robert F. Bonner , Thomas J. Pohida , Michael R. Emmert-Buck , Michael Anthony Tangrea , Rodrigo F. Chuaqui
发明人： Robert F. Bonner , Thomas J. Pohida , Michael R. Emmert-Buck , Michael Anthony Tangrea , Rodrigo F. Chuaqui
IPC分类号： B05D1/00
CPC分类号： G01N33/543 , G01N33/56966 , G01N2001/284 , Y10T428/2813
摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.
摘要翻译：一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑性层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及用于在照射期间向转印膜施加恒定压力的装置。

6. 发明授权

US08460744B2 Target activated microtransfer 有权
标题翻译：靶活化微转移
公开(公告)号：US08460744B2
公开(公告)日：2013-06-11
申请号：US12713105
申请日：2010-02-25
申请人： Robert F. Bonner , Thomas J. Pohida , Michael R. Emmert-Buck , Michael Anthony Tangrea , Rodrigo F. Chuaqui
发明人： Robert F. Bonner , Thomas J. Pohida , Michael R. Emmert-Buck , Michael Anthony Tangrea , Rodrigo F. Chuaqui
IPC分类号： B05D1/00
CPC分类号： G01N33/543 , G01N33/56966 , G01N2001/284 , Y10T428/2813
摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.
摘要翻译：一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及用于在照射期间向转印膜施加恒定压力的装置。

7. 发明授权

US07358347B1 MEN1, the gene associated with multiple endocrine neoplasia type 1, menin polypeptides and uses thereof 失效
标题翻译： MEN1，与多发性内分泌肿瘤1型有关的基因，menin多肽及其用途
公开(公告)号：US07358347B1
公开(公告)日：2008-04-15
申请号：US09380337
申请日：1998-03-04
申请人： Settara Chandrasekharappa , Siradanahalli Guru , Pachiappan Manickam , Francis S. Collins , Michael R. Emmert-Buck , Larisa V. Debelenko , Irina A. Lubensky , Lance A. Liotta , Sunita K. Agarwal , Allen M. Spiegel , A. Lee Burns , Stephen J. Marx , Zhengping Zhuang
发明人： Settara Chandrasekharappa , Siradanahalli Guru , Pachiappan Manickam , Francis S. Collins , Michael R. Emmert-Buck , Larisa V. Debelenko , Irina A. Lubensky , Lance A. Liotta , Sunita K. Agarwal , Allen M. Spiegel , A. Lee Burns , Stephen J. Marx , Zhengping Zhuang
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/68 , C12P19/26 , C12P19/34
CPC分类号： C12Q1/6886 , C12Q2600/156
摘要： The invention relates to the discovery of a novel tumor suppressor gene which is associated with multiple endocrine neoplasia type 1. The gene has been designated MEN1 and the gene product is menin. The absence of this protein and associated mutations in the corresponding gene have been identified in individuals suffering from multiple endocrine neoplasia type 1. The identification of this marker for multiple endocrine neoplasia type 1 has diagnostic uses as well as for gene therapy.
摘要翻译：本发明涉及一种与多发性内分泌肿瘤1型相关的新型肿瘤抑制基因的发现。该基因被命名为MEN1，该基因产物是menin。在具有1型多发性内分泌肿瘤1型的个体中已经鉴定了相应基因中不存在该蛋白质和相关突变。鉴定该多重内分泌肿瘤1型的该标志物具有诊断用途以及基因治疗。

8. 发明申请

US20140066752A1 Methods and Systems for Using Therapeutic, Diagnostic or Prophylactic Magnetic Agents 有权
标题翻译：使用治疗，诊断或预防性磁性药物的方法和系统
公开(公告)号：US20140066752A1
公开(公告)日：2014-03-06
申请号：US14075408
申请日：2013-11-08
申请人： Michael R. Emmert-Buck , Benjamin Shapiro
发明人： Benjamin Shapiro , Michael R. Emmert-Buck
IPC分类号： A61N2/00 , A61B5/055
CPC分类号： A61N2/00 , A61B5/055 , A61N1/406 , A61N2/02 , A61N2/06 , B33Y80/00
摘要： Systems and methods are disclosed for directing magnetizable particles comprising therapeutic agents to a target volume, or for guiding magnetizable particles comprising therapeutic agents from a first target volume to a second target volume, at a distance using a magnetic field, to enable the treatment of diseased areas including areas deep inside a patient's body. The methods may be used to diagnose or treat diseased areas within a patient, for example tumors of the lungs, intestines, and liver, and is also useful in enhancing the permeability of solid tumors to chemotherapeutic agents.
摘要翻译：公开了用于将包含治疗剂的可磁化颗粒引导至靶体积的系统和方法，或者使用磁场以一定距离将包含治疗剂的可磁化颗粒从第一目标体积引导至第二靶体积，以使患者能够治疗包括患者身体深处的区域。该方法可用于诊断或治疗患者内的患病区域，例如肺，肠和肝脏的肿瘤，并且还可用于增强实体瘤对化疗剂的渗透性。

9. 发明授权

US07214477B1 Layered device with capture regions for cellular analysis 有权
标题翻译：具有捕获区域的分层设备进行细胞分析
公开(公告)号：US07214477B1
公开(公告)日：2007-05-08
申请号：US10048194
申请日：2000-07-26
申请人： Michael R. Emmert-Buck
发明人： Michael R. Emmert-Buck
IPC分类号： C12Q1/00
CPC分类号： G01N33/54386
摘要： The present invention involves methods, systems, and devices for analyzing a biological material, such as a cellular or other specimen. The method includes placing the specimen on a substrate having different capture regions, such as contiguous layers, wherein the different capture regions of the substrate contain different identification molecules, and transferring components of the specimen through the capture regions under conditions that allow the components to interact with different identification molecules in the different regions of the substrate. The components of the specimen can be transferred through the different layers (or other regions) of the substrate by capillary action of a solution moving through the cellular specimen or by electrophoresis. The transfer of components of the specimen through the substrate may occur while maintaining a geometric correspondence to the specimen, such as the cytoarchitecture of a cellular specimen, for example by moving the components through parallel layers having positions that correspond to positions within the specimen. When the cellular architecture of the specimen is maintained, a correlation between the different identification molecules and the components of the cellular specimens may be made. The analysis can occur with one or more different discrete (for example cellular) specimens on a surface of the substrate. Examples of cellular specimens include, but are not limited to tissue sections, particularly tumor tissue sections. The cellular specimen can also include cultured cells or a cytology sample. Cytostat tissue sections cut slightly thicker than usual, that is about 25 to about 50 μm, improves the ability to detect molecules of moderate and low level abundance.
摘要翻译：本发明涉及用于分析生物材料（例如细胞或其他样品）的方法，系统和装置。该方法包括将样品放置在具有不同捕获区域（例如连续层）的基底上，其中衬底的不同捕获区域含有不同的识别分子，并且在允许组分相互作用的条件下将样品的组分转移通过捕获区域在基质的不同区域具有不同的识别分子。样品的组分可以通过移动通过细胞样品的溶液的毛细管作用或通过电泳转移通过衬底的不同层（或其他区域）。可以在保持与样品几何对应的情况下，例如细胞样品的细胞架构，例如通过将具有与样品中的位置相对应的位置的平行层移动组件，可以发生样品的通过基底的成分的转移。当维持样品的细胞结构时，可以制备不同识别分子与细胞样品组分之间的相关性。分析可以在衬底的表面上的一个或多个不同的离散（例如细胞）样品发生。细胞样品的实例包括但不限于组织切片，特别是肿瘤组织切片。细胞标本还可以包括培养的细胞或细胞学样品。细胞定型组织切片比通常切割的厚度大约为25至50微米，提高检测中等和低水平丰度的分子的能力。

10. 发明授权

US07838222B2 Methods, devices and kits for multiplex blotting of biological samples from multi-well plates 有权
标题翻译：用于从多孔板多次印迹生物样品的方法，装置和试剂盒
公开(公告)号：US07838222B2
公开(公告)日：2010-11-23
申请号：US11277227
申请日：2006-03-22
申请人： Vladimir Knezevic , Dan-Paul Hartmann , Michael R. Emmert-Buck
发明人： Vladimir Knezevic , Dan-Paul Hartmann , Michael R. Emmert-Buck
IPC分类号： G01N33/53 , G01N33/543
CPC分类号： G01N33/6845 , B01J19/0046 , B01J2219/00416 , B01J2219/00533 , B01J2219/00574 , B01J2219/00576 , B01J2219/00605 , B01J2219/0061 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00668 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01L3/50255 , B01L2300/0627 , B01L2300/0829 , B01L2400/049 , C40B40/06 , C40B40/10 , G01N33/54373 , G01N2500/00
摘要： The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
摘要翻译：本公开提供了允许在源自多样品保持器（例如多孔板）的样品中同时检测大量目标生物分子的方法，装置和试剂盒。一个具体的示例方法是制备多孔样品保持器的生物分子含量的多个实质性复制品的方法。还提供了用于执行所述方法的设备和套件。

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