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    • 6. 发明申请
    • DEVICE AND METHOD FOR ANALYZING STREPTAVIDIN
    • 用于分析STREPTAVIDIN的装置和方法
    • US20150086980A1
    • 2015-03-26
    • US14387431
    • 2013-03-21
    • Katsunori HoriiNaoto KanekoJou AkitomiShintaro KatoIwao Waga
    • Katsunori HoriiNaoto KanekoJou AkitomiShintaro KatoIwao Waga
    • G01N33/543G01N33/58G01N33/68C12N15/113C12N15/115
    • G01N33/54306C12N9/0065C12N15/113C12N15/115C12Y111/01007G01N33/542G01N33/58G01N33/68G01N2333/36
    • The present invention provides a novel sensor for detecting streptavidin (SA). The nucleic acid sensor for analyzing SA of the present invention includes the following nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to SA. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (SA), a loop-forming sequence (L2), and a stem-forming sequence (SD) linked in this order. In this nucleic acid element, in the absence of SA, the catalytic function of the catalyst nucleic acid molecule (D) is inhibited by stem formation in each of the stem-forming sequences (SA) and (SD), and in the presence of SA, the stem formation is released by a binding of the binding nucleic acid molecule (A) with the SA, and the catalytic function of the catalyst nucleic acid molecule (D) is exerted.
    • 本发明提供了用于检测链霉亲和素(SA)的新型传感器。 用于分析本发明的SA的核酸传感器包括以下核酸元件,其包含发挥催化功能的催化剂核酸分子(D)和与SA结合的结合核酸分子(A)。 核酸元件是包含第一链和第二链的双链核酸元件。 第一链(ss1)包括依次连接的结合核酸分子(A),环形成序列(L1)和催化剂核酸分子(D)。 第二链(ss2)包括以此顺序连接的茎形成序列(SA),环形成序列(L2)和茎形成序列(SD)。 在该核酸元件中,在不存在SA的情况下,催化剂核酸分子(D)的催化功能受到每个茎形成序列(SA)和(SD)中的茎形成的抑制,并且在 SA,通过结合核酸分子(A)与SA的结合释放茎的形成,并且发挥催化剂核酸分子(D)的催化功能。