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1. 发明申请

US20090142802A1 POLYPEPTIDES AND NUCLEIC ACIDS ENCODING SAME 审中-公开
标题翻译：多糖和核酸编码它们
公开(公告)号：US20090142802A1
公开(公告)日：2009-06-04
申请号：US11909195
申请日：2006-03-22
申请人： Peter Rahbek Oestergaard , Lene Nonboe Andersen , Aurelia Seon , Carlos Simoes-Nunes , Anna-Maria Klunter , Leonardo De Maria , Bjorn Eggert Christensen
发明人： Peter Rahbek Oestergaard , Lene Nonboe Andersen , Aurelia Seon , Carlos Simoes-Nunes , Anna-Maria Klunter , Leonardo De Maria , Bjorn Eggert Christensen
IPC分类号： C12P21/00 , C07K14/00 , C07H21/04 , C12N15/64
CPC分类号： C07K14/32 , A23K10/18 , A23K20/174 , A23K20/30
摘要： The present invention relates to isolated polypeptides and isolated nucleic acid sequences encoding the polypeptides, as well as methods for producing and using the polypeptides in animal feed. An example of a polypeptide of the invention is the so-called L12 protein from Bacillus licheniformis ATCC 14580 which improves the Feed Conversion Ratio (FCR) and/or acts as a gut microflora modulator. The invention furthermore relates to the probiotic use of strains of Bacillus which produce proteins related to L12.
摘要翻译：本发明涉及编码多肽的分离的多肽和分离的核酸序列，以及在动物饲料中产生和使用多肽的方法。本发明的多肽的实例是来自地衣芽孢杆菌ATCC 14580的所谓的L12蛋白，其改进饲料转化率（FCR）和/或作为肠道微生物调节剂。本发明还涉及产生与L12相关的蛋白质的芽孢杆菌菌株的益生菌利用。

2. 发明申请

US20120295000A1 Polypeptides and Nucleic Acids Encoding Same 审中-公开
标题翻译：编码相同的多肽和核酸
公开(公告)号：US20120295000A1
公开(公告)日：2012-11-22
申请号：US13566499
申请日：2012-08-03
申请人： Peter Rahbek Oestergaard , Lene Nonboe Anderson , Aurelia Séon , Carlos Simönes-Nunes , Anna-Maria Klünter , Leonardo De Maria , Bjorn Eggert Christensen
发明人： Peter Rahbek Oestergaard , Lene Nonboe Anderson , Aurelia Séon , Carlos Simönes-Nunes , Anna-Maria Klünter , Leonardo De Maria , Bjorn Eggert Christensen
IPC分类号： A23K1/16
CPC分类号： C07K14/32 , A23K10/18 , A23K20/174 , A23K20/30
摘要： The present invention relates to isolated polypeptides and isolated nucleic acid sequences encoding the polypeptides, as well as methods for producing and using the polypeptides in animal feed. An example of a polypeptide of the invention is the so-called L12 protein from Bacillus licheniformis ATCC 14580 which improves the Feed Conversion Ratio (FCR) and/or acts as a gut microflora modulator. The invention furthermore relates to the probiotic use of strains of Bacillus which produce proteins related to L12.
摘要翻译：本发明涉及编码多肽的分离的多肽和分离的核酸序列，以及在动物饲料中产生和使用多肽的方法。本发明的多肽的实例是来自地衣芽孢杆菌ATCC 14580的所谓的L12蛋白，其改进饲料转化率（FCR）和/或作为肠道微生物调节剂。本发明还涉及产生与L12相关的蛋白质的芽孢杆菌菌株的益生菌利用。

3. 发明申请

US20090238922A1 PROTEASES 有权
公开(公告)号：US20090238922A1
公开(公告)日：2009-09-24
申请号：US12466470
申请日：2009-05-15
申请人： Peter Rahbek Oestergaard , Leonardo De Maria
发明人： Peter Rahbek Oestergaard , Leonardo De Maria
IPC分类号： A23K1/165 , C12N9/52 , C12N15/11 , C12N15/00 , C12N1/21 , C12P21/02 , C11D7/42 , C12S9/00
CPC分类号： C12N9/58 , A01K2217/05 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g., in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
摘要翻译：本发明涉及肽类家族S2A或S1E的新一类丝氨酸蛋白酶，其在铜（Cu 2+）存在下是稳定的，和/或仅在有限程度上被铜抑制。还揭示了与此相关的结构特征。这类蛋白酶包括衍生自Brachysporiella gayana，Nocardiopsis dassonvillei subsp。的蛋白酶。 dassonvillei，Nocardiopsis prasina和Nocardiopsis alba，但不包括来源于Metarhizium anisopliae和Nocardiopsis sp的已知蛋白酶。 NRRL18262。本发明还涉及编码这种蛋白酶的DNA，其在宿主细胞中的表达，包括动物和植物细胞，以及其在动物饲料和洗涤剂中的用途。特别地，本发明涉及动物饲料和动物饲料添加剂，例如预混物，将这些蛋白酶与1-500ppm Cu（进料中浓度）一起掺入。

4. 发明申请

US20110124084A1 PROTEASES 审中-公开
公开(公告)号：US20110124084A1
公开(公告)日：2011-05-26
申请号：US13014974
申请日：2011-01-27
申请人： Peter Rahbek Oestergaard , Leonardo De Maria
发明人： Peter Rahbek Oestergaard , Leonardo De Maria
IPC分类号： C12N9/48 , C07H21/00 , C12N15/85 , C12N5/10 , A23K1/165 , C11D3/386
CPC分类号： C12N9/58 , A01K2217/05 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g., in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
摘要翻译：本发明涉及肽类家族S2A或S1E的新一类丝氨酸蛋白酶，其在铜（Cu 2+）存在下是稳定的，和/或仅在有限程度上被铜抑制。还揭示了与此相关的结构特征。这类蛋白酶包括衍生自Brachysporiella gayana，Nocardiopsis dassonvillei subsp。的蛋白酶。 dassonvillei，Nocardiopsis prasina和Nocardiopsis alba，但不包括来源于Metarhizium anisopliae和Nocardiopsis sp的已知蛋白酶。 NRRL18262。本发明还涉及编码这种蛋白酶的DNA，其在宿主细胞中的表达，包括动物和植物细胞，以及其在动物饲料和洗涤剂中的用途。特别地，本发明涉及动物饲料和动物饲料添加剂，例如预混物，将这些蛋白酶与1-500ppm Cu（进料中浓度）一起掺入。

5. 发明授权

US07906310B2 Proteases 有权
标题翻译：蛋白酶
公开(公告)号：US07906310B2
公开(公告)日：2011-03-15
申请号：US12466470
申请日：2009-05-15
申请人： Peter Rahbek Oestergaard , Leonardo De Maria
发明人： Peter Rahbek Oestergaard , Leonardo De Maria
IPC分类号： C12N9/52 , C12N15/57 , C12N15/74 , C12N15/75 , C12N15/80 , A23K1/165 , C11D3/386
CPC分类号： C12N9/58 , A01K2217/05 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g., in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
摘要翻译：本发明涉及肽类家族S2A或S1E的新一类丝氨酸蛋白酶，其在铜（Cu 2+）存在下是稳定的，和/或仅在有限程度上被铜抑制。还揭示了与此相关的结构特征。这类蛋白酶包括衍生自Brachysporiella gayana，Nocardiopsis dassonvillei subsp。的蛋白酶。 dassonvillei，Nocardiopsis prasina和Nocardiopsis alba，但不包括来源于Metarhizium anisopliae和Nocardiopsis sp的已知蛋白酶。 NRRL18262。本发明还涉及编码这种蛋白酶的DNA，其在宿主细胞中的表达，包括动物和植物细胞，以及其在动物饲料和洗涤剂中的用途。特别地，本发明涉及动物饲料和动物饲料添加剂，例如预混物，将这些蛋白酶与1-500ppm Cu（进料中浓度）一起掺入。

6. 发明授权

US07588926B2 Proteases 有权
标题翻译：蛋白酶
公开(公告)号：US07588926B2
公开(公告)日：2009-09-15
申请号：US10544861
申请日：2004-02-09
申请人： Peter Rahbek Oestergaard , Leonardo De Maria
发明人： Peter Rahbek Oestergaard , Leonardo De Maria
IPC分类号： C12N9/58 , C12N15/57 , C12N15/74 , C12N15/79 , C11D3/386 , A23K1/165
CPC分类号： C12N9/58 , A01K2217/05 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba. The invention also relates to DNA encoding such proteases and the expression thereof in a host cell, animal and plant cells, as well as to the use thereof in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
摘要翻译：本发明涉及肽类家族S2A或S1E的新一类丝氨酸蛋白酶，其在铜（Cu 2+）存在下是稳定的，和/或仅在有限程度上被铜抑制。还揭示了与此相关的结构特征。这类蛋白酶包括衍生自Brachysporiella gayana，Nocardiopsis dassonvillei subsp。的蛋白酶。 dassonvillei，Nocardiopsis prasina和Nocardiopsis alba。本发明还涉及编码这种蛋白酶的DNA及其在宿主细胞，动物和植物细胞中的表达，以及其在动物饲料和洗涤剂中的用途。特别地，本发明涉及动物饲料和动物饲料添加剂，例如预混物，将这些蛋白酶与1-500ppm Cu（进料中浓度）一起掺入。

7. 发明授权

US08557553B2 Nuclease reduction 有权
标题翻译：核酸酶减少
公开(公告)号：US08557553B2
公开(公告)日：2013-10-15
申请号：US12671973
申请日：2008-08-19
申请人： Kim Uhre Hansen , Peter Rahbek Oestergaard
发明人： Kim Uhre Hansen , Peter Rahbek Oestergaard
IPC分类号： C12N9/96 , C07K1/02 , C07K1/113 , C12N9/54
CPC分类号： C12N9/99 , C12N9/54 , C12N9/96
摘要： The present disclosure relates to a method for reducing nuclease activity in a subtilisin solution obtained from a fermentation broth including: a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5; b) adding a polyol; and c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C. In accordance with the present disclosure, the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 60% of the initial value.
摘要翻译：本公开涉及一种用于降低从发酵液获得的枯草杆菌蛋白酶溶液中的核酸酶活性的方法，包括：a）将枯草杆菌蛋白酶溶液的pH调节至pH在8.5至pH 10.5范围内的pH; b）加入多元醇; 和c）将枯草杆菌蛋白酶溶液的温度调节至50℃至80℃的温度。根据本公开，将核酸酶活性降低至小于初始值的5％，并且枯草杆菌蛋白酶活性维持在初始值的60％以上。

8. 发明申请

US20130102058A1 Dehairing of Skins and Hides 有权
标题翻译：脱皮皮肤和皮革
公开(公告)号：US20130102058A1
公开(公告)日：2013-04-25
申请号：US13702567
申请日：2011-06-22
申请人： Niels Henrik Soerensen , Tine Hoff , Peter Rahbek Oestergaard , Pierre Cassland
发明人： Niels Henrik Soerensen , Tine Hoff , Peter Rahbek Oestergaard , Pierre Cassland
IPC分类号： C14C1/06
CPC分类号： C14C1/065 , C14C1/04 , C14C1/08 , C14C3/00
摘要： The present invention relates to a method for the processing of hides or skins into leather, comprising enzymatic treatment of the hide or skin with carbohydrase in the soaking step. The present invention can achieve optimal fiber opening results in a relatively short period of time and at the same time does not cause loose grain, and meanwhile the pollution or impact on the environment is reduced in a maximum way.
摘要翻译：本发明涉及一种将皮革或皮肤加工成皮革的方法，包括在浸泡步骤中用碳水解酶酶处理皮肤或皮肤。本发明可以在相当短的时间内实现最佳的纤维开放结果，同时不会导致颗粒松散，同时最大程度地减少对环境的污染或对环境的影响。

9. 发明申请

US20100255153A1 PROCESS FOR MAKING A MILK-BASED PROTEIN HYDROLYSATE 有权
标题翻译：制备基于乳液的蛋白质水解产物的方法
公开(公告)号：US20100255153A1
公开(公告)日：2010-10-07
申请号：US12752207
申请日：2010-04-01
申请人： Peter Rahbek Oestergaard , Steffen Ernst , Gitte B. Lynglev
发明人： Peter Rahbek Oestergaard , Steffen Ernst , Gitte B. Lynglev
IPC分类号： A23J3/34 , A23C9/152 , A23J3/04 , A23J1/20 , A23L1/305
CPC分类号： A23L1/296 , A23L33/18 , A23L33/19 , A23L33/40 , C12N9/52 , C12N9/58 , C12N9/6424 , C12N9/6427
摘要： The present invention relates to an enzymatic process for making a milk-based protein hydrolysate and use of such hydrolysate, e.g., in an infant formula composition.
摘要翻译：本发明涉及制备基于乳的蛋白质水解产物的酶法和使用这种水解产物，例如在婴儿配方组合物中。

10. 发明申请

US20080286415A1 Proteases 有权
标题翻译：蛋白酶
公开(公告)号：US20080286415A1
公开(公告)日：2008-11-20
申请号：US11570913
申请日：2005-06-17
申请人： Soren Flensted Lassen , Carsten Sjoeholm , Peter Rahbek Oestergaard , Morten Fischer
发明人： Soren Flensted Lassen , Carsten Sjoeholm , Peter Rahbek Oestergaard , Morten Fischer
IPC分类号： A23K1/165 , C12N9/50 , C12N15/11 , C12N15/00 , C12N5/06 , C12N5/04 , A01H5/00 , A01K67/027 , C12P21/04 , C12N1/20
CPC分类号： C12N9/52 , A23J3/346 , A23K20/189 , A23K50/30 , A23K50/60 , A23K50/75 , C11D3/38609 , C11D3/38618
摘要： Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.
摘要翻译：衍生自Nocardiopsis dassonvillei subsp。 dassonvillei DSM 43235，Nocardiopsis prasina DSM 15649，Nocardiopsis prasina（以前的alba）DSM 14010 Nocardiopsis sp。 DSM 16424，Nocardiopsis alkaliphila DSM 44657和Nocardiopsis lucentensis DSM 44048以及同源蛋白酶; 其在各种宿主中的重组生产，包括转基因植物和非人类动物，以及它们在动物饲料和洗涤剂中的应用。蛋白酶是酸稳定的，碱稳定的和/或热稳定的。

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