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1. 发明申请

US20090142802A1 POLYPEPTIDES AND NUCLEIC ACIDS ENCODING SAME 审中-公开
标题翻译：多糖和核酸编码它们
公开(公告)号：US20090142802A1
公开(公告)日：2009-06-04
申请号：US11909195
申请日：2006-03-22
申请人： Peter Rahbek Oestergaard , Lene Nonboe Andersen , Aurelia Seon , Carlos Simoes-Nunes , Anna-Maria Klunter , Leonardo De Maria , Bjorn Eggert Christensen
发明人： Peter Rahbek Oestergaard , Lene Nonboe Andersen , Aurelia Seon , Carlos Simoes-Nunes , Anna-Maria Klunter , Leonardo De Maria , Bjorn Eggert Christensen
IPC分类号： C12P21/00 , C07K14/00 , C07H21/04 , C12N15/64
CPC分类号： C07K14/32 , A23K10/18 , A23K20/174 , A23K20/30
摘要： The present invention relates to isolated polypeptides and isolated nucleic acid sequences encoding the polypeptides, as well as methods for producing and using the polypeptides in animal feed. An example of a polypeptide of the invention is the so-called L12 protein from Bacillus licheniformis ATCC 14580 which improves the Feed Conversion Ratio (FCR) and/or acts as a gut microflora modulator. The invention furthermore relates to the probiotic use of strains of Bacillus which produce proteins related to L12.
摘要翻译：本发明涉及编码多肽的分离的多肽和分离的核酸序列，以及在动物饲料中产生和使用多肽的方法。本发明的多肽的实例是来自地衣芽孢杆菌ATCC 14580的所谓的L12蛋白，其改进饲料转化率（FCR）和/或作为肠道微生物调节剂。本发明还涉及产生与L12相关的蛋白质的芽孢杆菌菌株的益生菌利用。

2. 发明授权

US06677147B2 Pectate lyases 失效
标题翻译：果胶裂解酶
公开(公告)号：US06677147B2
公开(公告)日：2004-01-13
申请号：US10072152
申请日：2002-02-07
申请人： Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schroder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人： Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schroder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号： C12N988
CPC分类号： C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要： The present invention relates to pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) (SEQ ID NO: 2) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
摘要翻译：本发明涉及属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro（NLNSRVP）（SEQ ID NO：2）的果胶酸裂解酶在中性或碱性条件下的工业过程中具有良好的性能，例如洗涤和纺织加工。果胶裂解酶可以衍生自芽孢杆菌属物种。

3. 发明授权

US6001639A Endoglucanases 失效
标题翻译：内切葡聚糖酶
公开(公告)号：US6001639A
公开(公告)日：1999-12-14
申请号：US651136
申请日：1996-05-21
申请人： Martin Schulein , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Iium Nielsen , Michiko Ihara , Shinobu Takagi
发明人： Martin Schulein , Lene Nonboe Andersen , S.o slashed.ren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Iium Nielsen , Michiko Ihara , Shinobu Takagi
IPC分类号： C12N15/09 , C05F11/08 , C05F17/00 , C07G99/00 , C11D3/386 , C12N1/19 , C12N9/42 , C12N15/00 , C12R1/645 , C12R1/69 , C12R1/77 , C12R1/80 , C12S3/04 , C12S11/00 , D06M13/02 , D06M13/322 , D06M13/46 , D06M16/00 , D06P5/02 , D06P5/15 , D21C5/00 , D21H11/16 , D21H11/20 , D21H17/00 , D21H21/10 , D21C1/00
CPC分类号： C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H21/10 , C07K2319/00 , D21H17/005 , Y02P20/145 , Y02W30/43
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence consisting of 14 amino acid residues having the following sequenceThr Arg Xaa Xaa Asp Cys Cys Xaa Xaa (SEQ ID NO:79) 1 2 3 4 5 6 7 8 9 - Xaa Cys Xaa Trp Xaa 10 11 12 13 14 and a second amino acid sequence consisting of 5 amino acid residues having the following sequenceTrp Cys Cys Xaa Cys (SEQ ID NO:80) 1 2 3 4 5 wherein, in position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; in position 8 of the first sequence, the amino acid is Arg, Lys or His; in position 9, 10, 12 and 14, respectively, of the first sequence, and in position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue in position 12 is Ser, then the amino acid residue in position 14 is not Ser, and (ii) when the amino residue in position 12 is Gly, then the amino acid residue in position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
摘要翻译：本发明涉及基本上由具有纤维素分解活性的酶组成的酶制剂，其包含由具有以下序列的14个氨基酸残基组成的第一氨基酸序列：Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa（SEQ ID NO：79） - 1 2 3 4 5 6 7 8 9 - - Xaa Cys Xaa Trp Xaa - 10 11 12 13 14 - 和由具有以下序列的5个氨基酸残基组成的第二个氨基酸序列--Trp Cys Cys Xaa Cys（SEQ ID NO ：80） - 1 2 3 4 5 - 其中，在第一序列的位置3，氨基酸是Trp，Tyr或Phe; 在第一序列的位置4，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 分别位于第一序列的第9,10,12和14位，在第二序列的位置4中，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列，（i）当位置12的氨基残基为Ser时，14位的氨基酸残基不为Ser，（ii）当位置12的氨基残基为Gly时，位置14的氨基酸残基为 Ala在工业应用中表现非常好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或服装的磨损外观以及纸浆处理。此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。

4. 发明授权

US5858760A Enzyme with pectin lyase activity 失效
标题翻译：果胶裂解酶活性酶
公开(公告)号：US5858760A
公开(公告)日：1999-01-12
申请号：US887365
申请日：1997-07-02
申请人： Henrik Dalb.o slashed.ge , Lene Venke Kofod , Markus Sakari Kauppinen , Lene Nonboe Andersen , Stephan Christgau , Hans Peter Heldt-Hansen
发明人： Henrik Dalb.o slashed.ge , Lene Venke Kofod , Markus Sakari Kauppinen , Lene Nonboe Andersen , Stephan Christgau , Hans Peter Heldt-Hansen
IPC分类号： A61B19/04 , C12N9/88 , C12N15/60
CPC分类号： C12N9/88 , A61B42/40 , A61B42/50
摘要： The nucleic acid sequence encoding a pectin lyase from Aspergillus aculeatus, CBS 101.43 and the corresponding amino acid sequence are disclosed. The nucleic acid is used to transform a host cell which is utilized in a method to make the pectin lyase. The catalytic properties and stability characteristics of the enzyme are reported. The enzyme is useful for the degradation of plant cell wall components.
摘要翻译： PCT No.PCT / DK94 / 00105 Sec。 371日期：1995年9月26日 102（e）日期1995年9月26日PCT 1994年3月11日PCT公布。公开号WO94 / 21786 1994年9月29日，公开了从棘孢曲霉CBS 101.43和相应的氨基酸序列编码果胶裂解酶的核酸序列。核酸用于转化用于制备果胶裂解酶的方法中的宿主细胞。报道了酶的催化性质和稳定性。该酶可用于植物细胞壁组分的降解。

5. 发明授权

US5854050A Enzyme with protease activity 失效
标题翻译：具有蛋白酶活性的酶
公开(公告)号：US5854050A
公开(公告)日：1998-12-29
申请号：US578551
申请日：1996-02-01
申请人： Henrik Dalb.o slashed.ge , Stephan Christgau , Lene Nonboe Andersen , Lene Venke Kofod , Markus Sakari Kauppinen , Jack Bech Nielsen , Claus Dambmann
发明人： Henrik Dalb.o slashed.ge , Stephan Christgau , Lene Nonboe Andersen , Lene Venke Kofod , Markus Sakari Kauppinen , Jack Bech Nielsen , Claus Dambmann
IPC分类号： C12N1/21 , A21D2/26 , A21D8/04 , A23K20/189 , C11D3/00 , C11D3/386 , C11D3/39 , C12N1/15 , C12N5/04 , C12N9/62 , C12N9/82 , C12N15/09 , C12N15/57 , C12P21/06 , C12R1/685 , C12R1/69 , C07H21/04
CPC分类号： C11D3/3947 , A21D8/042 , A23K20/189 , C11D3/0078 , C11D3/386 , C11D3/38618 , C11D3/38636 , C12N9/62 , C12N9/82
摘要： A DNA construct comprising a DNA sequence encoding an enzyme exhibiting protease activity, which DNA sequence comprises the DNA sequence shown in SEQ ID No. 1 or 2 or an analog of any of these sequences being at least 80% homologous to the DNA sequence shown in SEQ ID No. 1 or 2. The proteases encoded by the DNA sequences have an acid pH optimum.
摘要翻译： PCT No.PCT / DK94 / 00274 Sec。 371日期：1996年2月1日 102（e）日期1996年2月1日PCT提交1994年7月5日PCT公布。 WO95 / 02044 PCT公开号日期1995年1月19日一种包含编码表现出蛋白酶活性的酶的DNA序列的DNA构建体，该DNA序列包含SEQ ID No.1或2所示的DNA序列或这些序列中的任一个的类似物与 DNA序列如SEQ ID No.1或2所示。由DNA序列编码的蛋白酶具有酸性pH最佳值。

6. 发明授权

US5817499A DNA encoding an enzyme with endoglucanase activity from Trichoderma harzianum 失效
标题翻译：编码具有来自哈茨木霉的内切葡聚糖酶活性的酶的DNA
公开(公告)号：US5817499A
公开(公告)日：1998-10-06
申请号：US578590
申请日：1996-01-03
申请人： Henrik Dalb.o slashed.ge , Stephan Christgau , Lene Nonboe Andersen , Lene Venke Kofod , Markus Sakari Kauppinen
发明人： Henrik Dalb.o slashed.ge , Stephan Christgau , Lene Nonboe Andersen , Lene Venke Kofod , Markus Sakari Kauppinen
IPC分类号： C12N9/42 , C12N15/56 , C12N5/10 , C12N9/24 , C12N21/02
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： DNA encoding an endoglucanase from Trichoderma harzianum is disclosed. The endoglucanase has activity toward mixed .beta.-1,3-1,4 glucans and is especially useful in brewing processes.
摘要翻译： PCT No.PCT / DK94 / 00275 Sec。 371日期1996年1月3日 102（e）日期1996年1月3日PCT 1994年7月5日PCT公布。公开号WO95 / 02043 1995年1月19日公开了编码哈茨木霉的内切葡聚糖酶的DNA。内切葡聚糖酶具有对混合的β-1,3-1,4葡聚糖的活性，并且在酿造过程中特别有用。

7. 发明授权

US07273745B2 Pectate lyases 失效
标题翻译：果胶裂解酶
公开(公告)号：US07273745B2
公开(公告)日：2007-09-25
申请号：US11605148
申请日：2006-11-28
申请人： Lene Nonboe Andersen , Hanne Dela, legal representative , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人： Martin Schülein, deceased
IPC分类号： C12N9/88 , D06M16/00
CPC分类号： C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要： The present invention relates to pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) (amino acids 236-242 of SEQ ID NO: 2) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
摘要翻译：本发明涉及属于多糖裂解酶家族1的氨基酸序列Asn Leu Asn Ser Arg Val Pro（NLNSRVP）（SEQ ID NO：2的氨基酸236-242）的果胶酸裂解酶在中性的工业过程中具有良好的性能或碱性条件如洗涤和纺织加工。果胶裂解酶可以衍生自芽孢杆菌属物种。

8. 发明授权

US07226773B2 Endoglucanases 有权
公开(公告)号：US07226773B2
公开(公告)日：2007-06-05
申请号：US10965499
申请日：2004-10-14
申请人： Martin Schulein, deceased , Torben Henriksen, legal representative , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
发明人： Martin Schulein, deceased , Torben Henriksen, legal representative , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
IPC分类号： C12N9/42 , D06M16/00 , C07G17/00 , C11D3/386 , C07H21/04
CPC分类号： C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa 1234567891011121314 and a second amino acid sequence having the following sequence TrpCysCysXaaCys(SEQ ID NO: 80) 12345 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

9. 发明授权

US06428993B1 Transglutaminase from oomycetes 失效
标题翻译：来自卵菌的转谷氨酰胺酶
公开(公告)号：US06428993B1
公开(公告)日：2002-08-06
申请号：US08881742
申请日：1997-06-24
申请人： Lisbeth Bech , Grethe Rasmussen , Torben Halkier , Mariko Okada , Lene Nonboe Andersen , Markus Sakari Kauppinen , Thomas Sandal
发明人： Lisbeth Bech , Grethe Rasmussen , Torben Halkier , Mariko Okada , Lene Nonboe Andersen , Markus Sakari Kauppinen , Thomas Sandal
IPC分类号： C12N910
CPC分类号： C12Y203/02013 , A21D8/042 , C12N9/1044 , C14C11/00 , Y10S435/911
摘要： The present invention relates to transglutaminases and transglutaminase preparations obtained from lower fungi belonging to the class Oomycetes. Unprecedented high-level expression is achievable by growing these coenocytium forming organisms, especially the strains Pythium sp., Pythium irregulare, Pythium dissotocum, Pythium periilum (or P. periplocum), Pythium torulosum, Pythium ultimum, Pythium aphanidermatum, Phytophthora cactorum, Phytophthora palmivora, Phytophthora porri, Phytophthora infestans, Phytoph-thora megasperma, Phytophthora cinnamomi and Phytophthora cryptogea. Arecombinant transglutaminase has been cloned and expressed, the enzyme and enzyme preparations being useful for cross-linking proteins, e.g. in flour, baked products, meat products, fish products, cosmetics, cheese, milk products, gelled food products and leather finishing, or as a glutaminase, e.g. in bread and other baked glutein-containing food products.
摘要翻译：本发明涉及从属于卵菌纲的较低真菌获得的转谷氨酰胺酶和转谷氨酰胺酶制剂。通过生长这些细胞形成的细菌，特别是菌株腐霉菌，不锈钢腐霉，腐霉腐霉（P. periplocum），腐皮腐，腐霉，腐霉，腐皮疫霉，致病疫霉（Phytophthora palmivora），可以实现前所未有的高水平表达，疫霉疫霉（Phytophthora porri），致病疫霉（Phytophthora infestans），致病疫霉（Phytoph-thora megasperma），肉桂疫霉（Phytophthora cinnamomi）和疫霉疫霉（Phytophthora cryptogea）。克隆和表达重组转谷氨酰胺酶，酶和酶制剂可用于交联蛋白质，例如，在面粉，烘焙食品，肉制品，鱼制品，化妆品，奶酪，乳制品，凝胶食品和皮革整理中，或作为谷氨酰胺酶。在面包和其他烘烤含麸质的食品中。

10. 发明授权

US06368843B1 Pectate lyases 有权
标题翻译：果胶裂解酶
公开(公告)号：US06368843B1
公开(公告)日：2002-04-09
申请号：US09694531
申请日：2000-10-23
申请人： Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
发明人： Lene Nonboe Andersen , Martin Schülein , Niels Erik Krebs Lange , Mads Eskelund Bjørnvad , Søren Møller , Sanne O. Schrøder Glad , Markus Sakari Kauppinen , Kirk Schnorr , Lars Kongsbak
IPC分类号： C12N926
CPC分类号： C12N9/2465 , A23K10/14 , A23K20/189 , C07K2319/20 , C11D3/386 , C12N9/88 , D06L4/40 , D06M16/003 , D21C5/005 , Y10T442/20
摘要： A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase may be derivable from Bacillus species.
摘要翻译：属于多糖裂解酶1族的氨基酸序列Asn Leu Asn Ser Arg Val Pro（NLNSRVP）的新一组果胶酸裂解酶在中性或碱性条件例如洗涤和纺织加工中在工业过程中具有良好的性能。果胶裂解酶可以衍生自芽孢杆菌属物种。

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