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1. 发明授权

US08642730B2 Endoglucanases 失效
标题翻译：内切葡聚糖酶
公开(公告)号：US08642730B2
公开(公告)日：2014-02-04
申请号：US12567302
申请日：2009-09-25
申请人： Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara , Torben Henriksen
发明人： Martin Schulein , Lene Nonboe Andersen , Soren Flensted Lassen , Markus Sakari Kauppinen , Lene Lange , Ruby Ilum Nielsen , Shinobu Takagi , Michiko Ihara
IPC分类号： C07K1/00
CPC分类号： C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1 2 3 4 5 6 7 8 9 10 11 12 Trp Xaa 13 14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1 2 3 4 5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
摘要翻译：本发明涉及基本上由具有纤维素分解活性的酶组成并包含具有以下序列的第一氨基酸序列的酶制剂（SEQ ID NO：79）Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 123456789101112 Trp Xaa 1314 和具有以下序列的第二氨基酸序列Trp Cys Cys Xaa Cys（SEQ ID NO：80）12345其中，在第一序列的3位，氨基酸为Trp，Tyr或Phe; 在第一序列的位置4处，氨基酸是Trp，Tyr或Phe; 在第一序列的位置8，氨基酸是Arg，Lys或His; 在第一序列的第9,10,12和14位，第2个序列的第4位，氨基酸是20个天然存在的氨基酸残基中的任一个，条件是在第一个氨基酸序列，（i）当第12位的氨基残基为Ser时，第14位的氨基酸残基不为Ser，（ii）当第12位的氨基残基为Gly时，第14位的氨基酸残基为 Ala在工业应用中表现良好，例如洗衣组合物，用于生物抛光新制造的纺织品，用于提供纤维素织物或衣服的磨损外观以及纸浆处理。此外，本发明涉及编码这些酶的DNA构建体，提供编码这种酶的基因的方法，生产酶的方法，含有这些酶的酶制剂，以及这些酶用于许多工业应用的用途。

2. 发明申请

US20130340122A1 Polypeptides having endoglucanase activity and polynucleotides encoding same 有权
公开(公告)号：US20130340122A1
公开(公告)日：2013-12-19
申请号：US13959577
申请日：2013-08-05
申请人： Soren Flensted Lassen , Paul Harris , Elena Vlasenko , Kristian Krogh
发明人： Soren Flensted Lassen , Paul Harris , Elena Vlasenko , Kristian Krogh
IPC分类号： C12N9/42
CPC分类号： C12N9/2434 , C12N9/2437 , C12Y302/01004
摘要： The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

3. 发明授权

US08119386B2 Proteases and variants thereof 失效
标题翻译：蛋白酶及其变体
公开(公告)号：US08119386B2
公开(公告)日：2012-02-21
申请号：US13014100
申请日：2011-01-26
申请人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
发明人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
IPC分类号： C12N9/54 , C11D3/386 , C12N15/57 , C12N15/74 , C12N15/75
CPC分类号： C12N9/54 , A61K38/00 , C11D3/386 , Y02A50/473
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译：本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。

4. 发明授权

US07910348B2 Polypeptides of Alicyclobacillus sp. having glutamic peptidase activity 失效
标题翻译：脂环酸杆菌多肽具有谷氨酰肽酶活性
公开(公告)号：US07910348B2
公开(公告)日：2011-03-22
申请号：US12185413
申请日：2008-08-04
申请人： Reinhard Wilting , Soren Flensted Lassen , Peter Rahbek Ostergaard
发明人： Reinhard Wilting , Soren Flensted Lassen , Peter Rahbek Ostergaard
IPC分类号： C12N9/48 , C07K14/00 , A23L1/305 , C11D1/00 , C12Q1/37 , C12P21/00
CPC分类号： C12N9/0004 , C07K14/195 , C07K14/32 , C12N9/18 , C12N9/2402 , C12N9/2437 , C12N9/244 , C12N9/52 , C12N9/86 , C12N9/90 , C12R1/01 , C12Y301/01058 , C12Y302/01096 , C12Y305/02006
摘要： Disclosed are isolated polypeptides of Alicyclobacillus sp. having glutamic peptidase activity.
摘要翻译：公开了脂环芽孢杆菌属的分离多肽。具有谷氨酰肽酶活性。

5. 发明申请

US20100107342A1 ENDOGLUCANASES 失效
公开(公告)号：US20100107342A1
公开(公告)日：2010-05-06
申请号：US12567302
申请日：2009-09-25
申请人： MARTIN SCHULEIN , TORBEN HENRIKSEN , LENE NONBOE ANDERSEN , SOREN FLENSTED LASSEN , MARKUS SAKARI KAUPPINEN , LENE LANGE , RUBY IIUM NIELSEN , SHINOBU TAKAGI , MICHIKO IHARA
发明人： MARTIN SCHULEIN , TORBEN HENRIKSEN , LENE NONBOE ANDERSEN , SOREN FLENSTED LASSEN , MARKUS SAKARI KAUPPINEN , LENE LANGE , RUBY IIUM NIELSEN , SHINOBU TAKAGI , MICHIKO IHARA
IPC分类号： C11D7/42 , C12N9/42
CPC分类号： C12N9/2437 , A61K38/00 , C05F11/08 , C05F17/0036 , C07K2319/00 , C11D3/386 , C12P19/14 , C12Y302/01004 , D06M16/003 , D06P5/02 , D06P5/158 , D21C5/005 , D21H17/005 , D21H21/10 , Y02P20/145 , Y02W30/43
摘要： The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1 2 3 4 5 6 7 8 9 10 11 12 Trp Xaa 13 14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1 2 3 4 5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

6. 发明申请

US20080219965A1 Phytase Variants 审中-公开
标题翻译：植酸酶变体
公开(公告)号：US20080219965A1
公开(公告)日：2008-09-11
申请号：US11942220
申请日：2007-11-19
申请人： Allan Svendsen , Soren Flensted Lassen , Dirk Kostrewa , Luis Pasamontes , Martin Lehmann , Andrea Tomschy , Adolphus Van Loon , Kurt Vogel , Markus Wyss
发明人： Allan Svendsen , Soren Flensted Lassen , Dirk Kostrewa , Luis Pasamontes , Martin Lehmann , Andrea Tomschy , Adolphus Van Loon , Kurt Vogel , Markus Wyss
IPC分类号： A61K38/46 , C12N9/14
CPC分类号： C12N15/8257 , A23K10/14 , A23K20/189 , C12N9/16 , C12N15/8242 , C12N15/8243 , Y10S435/911 , Y10S435/916
摘要： The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1, are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.
摘要翻译：本发明涉及植酸酶变体，其制备和用途，当植酸酶变体在根据图1对齐时。与在多个位置中的至少一个中的模型植酸酶相比，修改了1。优选的植酸酶类型是担子菌和子囊菌植酸酶，如Peniophora植酸酶和曲霉属植酸酶。优选的植酸酶变体表现出修正的活性特征，例如提高的比活性和/或改善的热稳定性。

7. 发明授权

US08357408B2 Proteases 有权
标题翻译：蛋白酶
公开(公告)号：US08357408B2
公开(公告)日：2013-01-22
申请号：US11570913
申请日：2005-06-17
申请人： Soren Flensted Lassen , Carsten Sjoholm , Peter Rahbek Ostergaard , Morten Fischer
发明人： Soren Flensted Lassen , Carsten Sjoholm , Peter Rahbek Ostergaard , Morten Fischer
IPC分类号： A23K1/165
CPC分类号： C12N9/52 , A23J3/346 , A23K20/189 , A23K50/30 , A23K50/60 , A23K50/75 , C11D3/38609 , C11D3/38618
摘要： Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.
摘要翻译：衍生自Nocardiopsis dassonvillei subsp。 dassonvillei DSM 43235，Nocardiopsis prasina DSM 15649，Nocardiopsis prasina（以前的alba）DSM 14010 Nocardiopsis sp。 DSM 16424，Nocardiopsis alkaliphila DSM 44657和Nocardiopsis lucentensis DSM 44048以及同源蛋白酶; 其在各种宿主中的重组生产，包括转基因植物和非人类动物，以及它们在动物饲料和洗涤剂中的应用。蛋白酶是酸稳定的，碱稳定的和/或热稳定的。

8. 发明授权

US07485447B2 Proteases and methods for producing them 有权
标题翻译：蛋白酶及其制备方法
公开(公告)号：US07485447B2
公开(公告)日：2009-02-03
申请号：US10560414
申请日：2004-06-21
申请人： Soren Flensted Lassen
发明人： Soren Flensted Lassen
IPC分类号： C12N9/52 , C12N15/57 , C12N15/62 , C12N15/74 , C12N15/75 , C12N15/82 , A23K1/175
CPC分类号： C12N9/52
摘要： A secreted proteolytic polypeptide comprising at least three non-polar or uncharged polar amino acids within the last four amino acids of the C-terminus of the polypeptide, encoding polynucleotides, expression vectors comprising the polynucleotides, host cell comprising the polynucleotides, methods for producing said polypeptide, and methods for using the polypeptide.
摘要翻译：一种分泌的蛋白水解多肽，其包含多肽的C-末端的最后四个氨基酸中的至少三个非极性或不带电极性氨基酸，编码多核苷酸，包含多核苷酸的表达载体，包含多核苷酸的宿主细胞，产生所述多核苷酸的方法多肽，以及使用该多肽的方法。

9. 发明申请

US20080220118A1 Phytase Variants 审中-公开
公开(公告)号：US20080220118A1
公开(公告)日：2008-09-11
申请号：US11942242
申请日：2007-11-19
申请人： Allan Svendsen , Soren Flensted Lassen , Dirk Kostrewa , Luis Pasamontes , Martin Lehman , Andrea Tomschy , Adolphus Van Loon , Kurt Vogel , Markus Wyss
发明人： Allan Svendsen , Soren Flensted Lassen , Dirk Kostrewa , Luis Pasamontes , Martin Lehman , Andrea Tomschy , Adolphus Van Loon , Kurt Vogel , Markus Wyss
IPC分类号： A23K1/18 , C12N9/14 , A23J1/00
CPC分类号： C12N15/8257 , A23K10/14 , A23K20/189 , C12N9/16 , C12N15/8242 , C12N15/8243 , Y10S435/911 , Y10S435/916
摘要： The present invention relates to phytase variants, their preparation and uses, which phytase variants, when aligned according to FIG. 1, are amended as compared to a model phytase in at least one of a number of positions. Preferred model phytases are basidiomycete and ascomycete phytases, such as Peniophora phytase and Aspergillus phytases. Preferred phytase variants exhibits amended activity characteristics, such as improved specific activity and/or improved thermostability.

10. 发明授权

US07238350B2 Phytase polypeptides 失效
标题翻译：植酸酶多肽
公开(公告)号：US07238350B2
公开(公告)日：2007-07-03
申请号：US09999214
申请日：2001-11-30
申请人： Soren Flensted Lassen , Lisbeth Bech , Anders Ohmann , Jens Breinholt , Claus Crone Fuglsang , Peter Rahbek Ostergaard
发明人： Soren Flensted Lassen , Lisbeth Bech , Anders Ohmann , Jens Breinholt , Claus Crone Fuglsang , Peter Rahbek Ostergaard
IPC分类号： A61K38/46 , A61K38/43 , C12N9/00 , C12N9/16 , C07K1/00
CPC分类号： C12Y301/03026 , A23K20/189 , A23L5/25 , A23L29/06 , A63B57/00 , A63B57/357 , A63B57/40 , C12N9/16 , C12P3/00
摘要： The present invention relates to isolated polypeptides having phytase activity, the corresponding cloned DNA sequences, a process for preparing such polypeptides, and the use thereof for a number of industrial applications. In particular, the invention relates to phytases derived from the phyllum Basidiomycota, phytases of certain consensus sequences and fungal 6-phytases.
摘要翻译：本发明涉及具有植酸酶活性的分离的多肽，相应的克隆的DNA序列，制备该多肽的方法及其在许多工业应用中的应用。特别地，本发明涉及源自担子菌的植酸酶，某些共有序列的植酸酶和真菌6-肌醇六磷酸酶。

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