会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 1. 发明授权
    • Endoglucanases
    • 内切葡聚糖酶
    • US08642730B2
    • 2014-02-04
    • US12567302
    • 2009-09-25
    • Lene Nonboe AndersenSoren Flensted LassenMarkus Sakari KauppinenLene LangeRuby Ilum NielsenShinobu TakagiMichiko IharaTorben Henriksen
    • Martin SchuleinLene Nonboe AndersenSoren Flensted LassenMarkus Sakari KauppinenLene LangeRuby Ilum NielsenShinobu TakagiMichiko Ihara
    • C07K1/00
    • C12N9/2437A61K38/00C05F11/08C05F17/0036C07K2319/00C11D3/386C12P19/14C12Y302/01004D06M16/003D06P5/02D06P5/158D21C5/005D21H17/005D21H21/10Y02P20/145Y02W30/43
    • The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
    • 本发明涉及基本上由具有纤维素分解活性的酶组成并包含具有以下序列的第一氨基酸序列的酶制剂(SEQ ID NO:79)Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 123456789101112 Trp Xaa 1314 和具有以下序列的第二氨基酸序列Trp Cys Cys Xaa Cys(SEQ ID NO:80)12345其中,在第一序列的3位,氨基酸为Trp,Tyr或Phe; 在第一序列的位置4处,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 在第一序列的第9,10,12和14位,第2个序列的第4位,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当第12位的氨基残基为Ser时,第14位的氨基酸残基不为Ser,(ii)当第12位的氨基残基为Gly时,第14位的氨基酸残基为 Ala在工业应用中表现良好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或衣服的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。