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    • 1. 发明授权
    • Enzyme preparation with cellulytic activity
    • 酶制剂具有纤维素分解活性
    • US5972872A
    • 1999-10-26
    • US870996
    • 1997-06-06
    • Lene LangeJack Bech NielsenMartin Schulein
    • Lene LangeJack Bech NielsenMartin Schulein
    • C12N9/42C11D3/386C11D11/00C12R1/00C12S3/08C12S11/00D06M16/00D06P5/02D06P5/15D21C5/00D21C5/02D21C9/00D21H17/00D21H21/10
    • D21H21/10C11D3/38645D06M16/003D06P5/02D06P5/158D21C5/005D21C5/027D21H17/005Y02W30/648
    • The present invention relates to detergent compositions comprising an enzyme preparation with substantial cellulytic activity, especially endoglucanase activity, at alkaline conditions which is enzyme composition is derived from or producible by a fungus selected from the Basidiomycetous families Coprinaceae and Bolbitiaceae, preferably from the group of strains belonging to the genera Psathyrella, Podaxis, Panaeolus, Coprinus and Bolbitius, more preferably from the group of strains belonging to the species Coprinus micaceus, Coprinus domesticus, Coprinus ephemerus, Coprinus disseminatus, Coprinus radians, Coprinus picaceus, Coprinus frisei, Coprinus subimpatiens, Psathyrella candolleana, Psathyrella prona, Panaeolus semiovatus, Podaxis pistillaris and Bolbitius aleuriatus; and a method for providing a stone-washed look to textiles, especially to cellulosic fabric such as denim, using the mentioned enzyme preparation; and a method for improving the drainage of paper pulp or the de-inking of recycled paper using the mentioned enzyme preparation; and an enzyme preparation with substantial cellulytic activity at alkaline conditions which is enzyme composition is derived from or producible by a fungus selected from the Basidiomycetous families Coprinaceae and Bolbitiaceae, provided that the fungus does not belong to the species Coprinus cinereus.
    • 本发明涉及洗涤剂组合物,其包含在碱性条件下具有显着的纤维素分解活性,特别是内切葡聚糖酶活性的酶制剂,其是酶组合物衍生自或可由选自以下的真菌中产生的真菌:来自巴斯德氏酵母属家蝇科和博比氏菌科,优选来自菌株组 属于Psathyrella,Podaxis,Panaeolus,Coprinus和Bolbitius属,更优选属于属于Coprinus micaceus,Coprinus domesticus,Coprinus ephemerus,Coprinus propaginatus,Coprinus radians,Coprinus picaceus,Coprinus frisei,Coprinus subimpatiens,Psathyrella 花椰菜,Psathyrella话,Panaeolus semiovatus,Podaxis pistillaris和Bolbitius aleuriatus; 以及使用所述酶制剂向纺织品,特别是纤维素织物如牛仔布提供经过洗涤的外观的方法; 以及使用所述酶制剂改进纸浆排出或再循环纸的脱墨的方法; 以及在碱性条件下具有显着的纤维素分解活性的酶制剂,其酶组成来自选自以下的真菌的产生或可生产,所述真菌选自巴斯德氏酵母属科罗班菌科和Bolbitiaceae,条件是该真菌不属于灰霉菌属(Coprinus cinereus)。
    • 8. 发明授权
    • Endoglucanases
    • 内切葡聚糖酶
    • US08642730B2
    • 2014-02-04
    • US12567302
    • 2009-09-25
    • Lene Nonboe AndersenSoren Flensted LassenMarkus Sakari KauppinenLene LangeRuby Ilum NielsenShinobu TakagiMichiko IharaTorben Henriksen
    • Martin SchuleinLene Nonboe AndersenSoren Flensted LassenMarkus Sakari KauppinenLene LangeRuby Ilum NielsenShinobu TakagiMichiko Ihara
    • C07K1/00
    • C12N9/2437A61K38/00C05F11/08C05F17/0036C07K2319/00C11D3/386C12P19/14C12Y302/01004D06M16/003D06P5/02D06P5/158D21C5/005D21H17/005D21H21/10Y02P20/145Y02W30/43
    • The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence (SEQ ID NO: 79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 1   2   3   4   5   6   7   8   9   10  11  12 Trp Xaa 13  14 and a second amino acid sequence having the following sequence Trp Cys Cys Xaa Cys(SEQ ID NO: 80) 1   2   3   4   5 wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.
    • 本发明涉及基本上由具有纤维素分解活性的酶组成并包含具有以下序列的第一氨基酸序列的酶制剂(SEQ ID NO:79)Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa Xaa Cys Xaa 123456789101112 Trp Xaa 1314 和具有以下序列的第二氨基酸序列Trp Cys Cys Xaa Cys(SEQ ID NO:80)12345其中,在第一序列的3位,氨基酸为Trp,Tyr或Phe; 在第一序列的位置4处,氨基酸是Trp,Tyr或Phe; 在第一序列的位置8,氨基酸是Arg,Lys或His; 在第一序列的第9,10,12和14位,第2个序列的第4位,氨基酸是20个天然存在的氨基酸残基中的任一个,条件是在第一个氨基酸序列 ,(i)当第12位的氨基残基为Ser时,第14位的氨基酸残基不为Ser,(ii)当第12位的氨基残基为Gly时,第14位的氨基酸残基为 Ala在工业应用中表现良好,例如洗衣组合物,用于生物抛光新制造的纺织品,用于提供纤维素织物或衣服的磨损外观以及纸浆处理。 此外,本发明涉及编码这些酶的DNA构建体,提供编码这种酶的基因的方法,生产酶的方法,含有这些酶的酶制剂,以及这些酶用于许多工业应用的用途。