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    • 8. 发明授权
    • Xyloglucan-specific alkaline xyloglucanase from bacillus
    • 来自芽孢杆菌的葡聚糖特异性碱性木葡聚糖酶
    • US06268197B1
    • 2001-07-31
    • US09110959
    • 1998-07-07
    • Martin SchuleinHelle OuttrupPer Lina JorgensenMads Eskelund Bjornvad
    • Martin SchuleinHelle OuttrupPer Lina JorgensenMads Eskelund Bjornvad
    • C12N942
    • C12N9/2434C11D3/38636
    • A xyloglucanase having a relative xyloglucanase activity of at least 50% at pH 7 and either no or an insignificant cellulolytic activity is obtainable e.g. from a strain of Bacillus. A xyloglucanase comprising an amino acid sequence as shown in positions 30-261 of SEQ ID NO:2 or homologues may be derived from eg Bacillus licheniformis, ATCC 14580, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 88 to nucleotide 783; and a xyloglucanase comprising an amino acid sequence as shown in positions 1-537 of SEQ ID NO:4 or homologues may be derived from eg B. agaradhaerens, NCIMB 40482, and may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:3 from nucleotide 1 to nucleotide 1611. The xyloglucanases are useful e.g. in cleaning compositions and for treatment of cellulosic fibres.
    • 在pH7下具有至少50%的相对木葡聚糖酶活性和无或不显着的纤维素分解活性的木葡聚糖酶可以例如获得。 来自芽孢杆菌菌株。 包含SEQ ID NO:2的位置30-261所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如地衣芽孢杆菌ATCC 14580,并且可以由包含如SEQ ID NO所示的核苷酸序列的多核苷酸分子编码 :1个从核苷酸88到核苷酸783; 并且包含SEQ ID NO:4的位置1-537所示的氨基酸序列或同源物的木葡聚糖酶可以衍生自例如琼脂糖琼脂糖凝胶,NCIMB 40482,并且可以由包含SEQ ID NO:4所示的核苷酸序列的多核苷酸分子编码 SEQ ID NO:3从核苷酸1到核苷酸1611.木葡聚糖酶是有用的,例如 在清洁组合物和纤维素纤维的处理中。