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1. 发明申请

US20080193940A1 SYSTEMS AND METHODS FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：用于检测核酸的系统和方法
公开(公告)号：US20080193940A1
公开(公告)日：2008-08-14
申请号：US11965807
申请日：2007-12-28
申请人： Vissarion Aivazachvili , Kristian Scaboo , Eugene Spier
发明人： Vissarion Aivazachvili , Kristian Scaboo , Eugene Spier
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6825 , C12Q1/6827 , C12Q2531/113 , C12Q2521/307 , C12Q2525/161 , C12Q2525/301 , C12Q2565/607 , C12Q2565/519 , C12Q2521/301 , C12Q2561/109
摘要： A method and kit for detecting a target nucleic acid in a sample is described. The sample to be analyzed may include a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising exonuclease activity that can cleave the hybridized hybridization probe to thereby generate a labeled probe fragment. At least one portion of the hybridization probe hybridizes to another portion of the hybridization probe to thereby form a folded structure. The method can involve melting the sample, reducing the temperature of the sample to allow primer and probe to each hybridize to at least a portion of single stranded target nucleic acid in the sample, elongating the primer and releasing the labeled probe fragment. The sample can be contacted with a solid support comprising surface bound capture probes which hybridize to the labeled probe fragments. The label can then be detected.
摘要翻译：描述了用于检测样品中靶核酸的方法和试剂盒。要分析的样品可以包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测标记的第二区，扩增杂交引物的聚合酶和包含外切核酸酶活性的酶，其可以切割杂交的杂交探针，从而产生标记的探针片段。至少一部分杂交探针与杂交探针的另一部分杂交，从而形成折叠结构。该方法可以包括熔化样品，降低样品的温度以使引物和探针各自与样品中的至少一部分单链靶核酸杂交，延长引物并释放标记的探针片段。样品可以与包含与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。然后可以检测标签。

2. 发明授权

US06503743B1 Isolated nucleic acid encoding a human lactate dehydrogenase and uses thereof 失效
标题翻译：分离的编码人乳酸脱氢酶的核酸及其用途
公开(公告)号：US06503743B1
公开(公告)日：2003-01-07
申请号：US09711681
申请日：2000-11-09
申请人： Steven I. Ladunga , Eugene Spier , Maureen Higgins , Simon Greenberg , Yu Wang
发明人： Steven I. Ladunga , Eugene Spier , Maureen Higgins , Simon Greenberg , Yu Wang
IPC分类号： C12N904
CPC分类号： C07K14/47
摘要： The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the secreted peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the secreted peptides, and methods of identifying modulators of the secreted peptides.
摘要翻译：本发明提供由人基因组内的基因编码的肽的氨基酸序列，本发明的分泌肽。本发明特别提供分离的肽和核酸分子，鉴定分泌肽的直向同源物和旁系同源物的方法，以及鉴定分泌肽的调节剂的方法。

3. 发明申请

US20050282162A1 Methods of validating snps and compiling libraries of assays 审中-公开
标题翻译：验证snps和编制测定文库的方法
公开(公告)号：US20050282162A1
公开(公告)日：2005-12-22
申请号：US10502761
申请日：2003-01-27
申请人： Francisco De La Vega , Janet Ziegle , Hadar Isaac , Charles Scafe , Eugene Spier
发明人： Francisco De La Vega , Janet Ziegle , Hadar Isaac , Charles Scafe , Eugene Spier
IPC分类号： G01N21/78 , B01L3/00 , B01L3/14 , C07H21/04 , C12M1/00 , C12N15/09 , C12P19/34 , C12Q1/68 , G01N20060101 , G01N27/62 , G01N30/88 , G01N33/48 , G01N33/50 , G01N35/00 , G06F20060101 , G06F17/00 , G06F17/30 , G06F19/20 , G06F19/22 , G06F19/26 , G06F19/28 , G06Q30/06 , G06Q50/22 , H04L12/16 , G06F19/00
CPC分类号： C12Q1/6858 , B01L3/545 , B01L3/5453 , B01L2300/021 , C12Q2600/156 , G01N35/00663 , G06Q30/0621 , G06Q30/0633 , G06Q30/0641 , G06Q50/22 , G16B25/00 , G16B30/00 , G16B35/00 , G16B45/00 , G16B50/00 , G16C20/60 , C12Q2547/101
摘要： Libraries of assays and methods of compiling the libraries are provided. The assays can identify Single Nucleotide Polymorphisms (SNPs). Methods of validating SNPs are provided. Methods of constructing linkage disequilibrium maps using sets or subsets of SNPs are also provided.
摘要翻译：提供了分析库和编译库的方法。测定可以鉴定单核苷酸多态性（SNP）。提供验证SNP的方法。还提供了使用SNP集合或子集构建连锁不平衡图的方法。

4. 发明申请

US20110212846A1 Methods and compositions for universal detection of nucleic acids 有权
标题翻译：普遍检测核酸的方法和组合物
公开(公告)号：US20110212846A1
公开(公告)日：2011-09-01
申请号：US12931803
申请日：2011-02-09
申请人： Eugene Spier
发明人： Eugene Spier
IPC分类号： C40B30/00 , C12Q1/68
CPC分类号： C12Q1/6853 , C12N2310/141 , C12Q1/686 , C12Q2565/107 , C12Q2525/301 , C12Q2525/155 , C12Q2565/1015
摘要： Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest. A variety of universal segment linking strategies are provided, including preamplification by polymerase chain reaction, ligation-based strategies, reverse transcription and linear polymerase extension. Linking the universal segments into a single molecule generates a tagged target nucleic acid which is detected in a manner dependent on an intramolecular interaction between one universal segment and a second portion of the tagged target nucleic acid. In certain embodiments, the intramolecular interaction includes the formation of a hairpin having a stem between a universal segment at one end of the tagged target nucleic acid and a second universal segment at the opposite end of the tagged target nucleic acid. A variety of detection formats are provided, including solution-phase and surface-based formats. The methods and compositions are well-suited for highly multiplexed nucleic acid detection, and are applicable for the detection of any target nucleic acid of interest in both research and clinical settings.
摘要翻译：提供了用于检测样品中一种或多种靶核酸的存在或量的方法和组合物。本发明的方法包括将通用核酸片段连接在依赖目的靶核酸的连接反应中的单个分子中。提供了各种通用片段连接策略，包括通过聚合酶链反应进行预扩增，基于连接的策略，逆转录和线性聚合酶扩增。将通用片段连接成单个分子产生标记的靶核酸，其以取决于标记的靶核酸的一个通用片段和第二部分之间的分子内相互作用的方式检测。在某些实施方案中，分子内相互作用包括在标记的靶核酸的一端的通用片段与标记的靶核酸的相对端的第二通用片段之间形成具有茎的发夹。提供了各种检测格式，包括溶液相和基于表面的格式。所述方法和组合物非常适用于高度多重化的核酸检测，并且适用于在研究和临床环境中检测目标靶核酸。

5. 发明申请

US20080038727A1 MicroRNA and Messenger RNA Detection on Arrays 审中-公开
标题翻译：微阵列和信使RNA检测阵列
公开(公告)号：US20080038727A1
公开(公告)日：2008-02-14
申请号：US11562359
申请日：2006-11-21
申请人： Eugene Spier
发明人： Eugene Spier
IPC分类号： C07H21/02 , C12M1/00 , C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6865 , C12Q2525/301 , C12Q2525/143 , C12Q2525/207 , C12Q2565/501
摘要： The present teachings provide methods for reverse transcribing, and detecting, a plurality of small nucleic acids such as micro RNAs, from the same reaction mixture as a plurality of messenger RNAs. High levels of multiplexing are provided by the use of a plurality of zip-coded stem-loop reverse transcription primers, along with an oligo-dT-promoter-containing reverse transcription primer, in the same reverse transcription reaction mixture. The resulting products can be amplified in an in vitro transcription reaction, and detected on a solid support such as an array. The present teachings also provide compositions, kits, and devices for performing and detecting the reverse transcription reactions described herein.
摘要翻译：本教导提供了与多个信使RNA相同的反应混合物逆转录和检测多个小核酸如微RNA的方法。通过在相同的逆转录反应混合物中使用多个zip编码的茎环逆转录引物以及含有含有dT启动子的逆转录引物提供高水平的复用。所得产物可以在体外转录反应中扩增，并在固体支持物如阵列上检测。本教导还提供用于进行和检测本文所述的逆转录反应的组合物，试剂盒和装置。

6. 发明申请

US20070141610A1 Methods, Compositions, and Kits for Forming Self-complementary Polynucleotides 有权
标题翻译：用于形成自身互补多核苷酸的方法，组合物和试剂盒
公开(公告)号：US20070141610A1
公开(公告)日：2007-06-21
申请号：US11622991
申请日：2007-01-12
申请人： Eugene Spier
发明人： Eugene Spier
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6813 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6876 , C12Q2561/125 , C12Q2525/301 , C12Q2525/161 , C12Q2525/155 , C12Q2521/501 , C12Q2525/307
摘要： The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a hairpin linker to the self-complementary ligation product can form a loop ligation product. In some embodiments, the loop ligation product can be amplified with rolling circle amplification. Detection of a loop ligation product can serve to determine the identity of a target polynucleotide.
摘要翻译：本教导通常涉及用于检测靶多核苷酸序列的方法，试剂盒和组合物。该教导还涉及产生自身互补多核苷酸产物的连接和扩增反应。在一些实施方案中，使用导致形成自交联结合产物的探针进行连接反应。将发夹接头连接到自交联结合产物上可以形成环结合产物。在一些实施方案中，可以用滚环扩增扩增环结合产物。环路连接产物的检测可以用于确定靶多核苷酸的身份。

7. 发明申请

US20060194225A1 Methods, compositions, and kits for forming self-complementary polynucleotides 有权
公开(公告)号：US20060194225A1
公开(公告)日：2006-08-31
申请号：US11305699
申请日：2005-12-16
申请人： Eugene Spier
发明人： Eugene Spier
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6876 , C12Q2561/125 , C12Q2525/301 , C12Q2525/161 , C12Q2525/155 , C12Q2521/501 , C12Q2525/307
摘要： The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a hairpin linker to the self-complementary ligation product can form a loop ligation product. In some embodiments, the loop ligation product can be amplified with rolling circle amplification. Detection of a loop ligation product can serve to determine the identity of a target polynucleotide.

8. 发明授权

US06753174B2 Isolated human lactate dehydrogenase proteins 失效
标题翻译：分离的人乳酸脱氢酶蛋白
公开(公告)号：US06753174B2
公开(公告)日：2004-06-22
申请号：US10274266
申请日：2002-10-21
申请人： Steven I. Ladunga , Eugene Spier , Maureen Higgins , Simon Greenberg , Yu Wang
发明人： Steven I. Ladunga , Eugene Spier , Maureen Higgins , Simon Greenberg , Yu Wang
IPC分类号： C12N904
CPC分类号： C07K14/47
摘要： The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the secreted peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the secreted peptides, and methods of identifying modulators of the secreted peptides.
摘要翻译：本发明提供由人基因组内的基因编码的肽的氨基酸序列，本发明的分泌肽。本发明特别提供分离的肽和核酸分子，鉴定分泌肽的直向同源物和旁系同源物的方法，以及鉴定分泌肽的调节剂的方法。

9. 发明授权

US08940487B2 Methods and compositions for universal detection of nucleic acids 有权
标题翻译：普遍检测核酸的方法和组合物
公开(公告)号：US08940487B2
公开(公告)日：2015-01-27
申请号：US12931803
申请日：2011-02-09
申请人： Eugene Spier
发明人： Eugene Spier
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6853 , C12N2310/141 , C12Q1/686 , C12Q2565/107 , C12Q2525/301 , C12Q2525/155 , C12Q2565/1015
摘要： Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest. A variety of universal segment linking strategies are provided, including preamplification by polymerase chain reaction, ligation-based strategies, reverse transcription and linear polymerase extension. Linking the universal segments into a single molecule generates a tagged target nucleic acid which is detected in a manner dependent on an intramolecular interaction between one universal segment and a second portion of the tagged target nucleic acid. In certain embodiments, the intramolecular interaction includes the formation of a hairpin having a stem between a universal segment at one end of the tagged target nucleic acid and a second universal segment at the opposite end of the tagged target nucleic acid. A variety of detection formats are provided, including solution-phase and surface-based formats. The methods and compositions are well-suited for highly multiplexed nucleic acid detection, and are applicable for the detection of any target nucleic acid of interest in both research and clinical settings.
摘要翻译：提供了用于检测样品中一种或多种靶核酸的存在或量的方法和组合物。本发明的方法包括将通用核酸片段连接在依赖目的靶核酸的连接反应中的单个分子中。提供了各种通用片段连接策略，包括通过聚合酶链反应进行预扩增，基于连接的策略，逆转录和线性聚合酶扩增。将通用片段连接成单个分子产生标记的靶核酸，其以取决于标记的靶核酸的一个通用片段和第二部分之间的分子内相互作用的方式检测。在某些实施方案中，分子内相互作用包括在标记的靶核酸的一端的通用片段与标记的靶核酸的相对端的第二通用片段之间形成具有茎的发夹。提供了各种检测格式，包括溶液相和基于表面的格式。所述方法和组合物非常适用于高度多重化的核酸检测，并且适用于在研究和临床环境中检测目标靶核酸。

10. 发明申请

US20070219364A1 Methods and Compositions for Detecting Targets 审中-公开
标题翻译：检测目标的方法和组成
公开(公告)号：US20070219364A1
公开(公告)日：2007-09-20
申请号：US11615626
申请日：2006-12-22
申请人： Mark Andersen , Michael Hunkapiller , Kenneth Livak , Eugene Spier , H. Wenz
发明人： Mark Andersen , Michael Hunkapiller , Kenneth Livak , Eugene Spier , H. Wenz
IPC分类号： C07H21/00
CPC分类号： C12Q1/6827 , C12Q2561/101 , C12Q2531/137 , C12Q2525/161
摘要： The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.
摘要翻译：本发明涉及使用连接和扩增检测靶核酸序列的存在或不存在（或定量）的方法和试剂盒。本发明还涉及采用可寻址部分和标记探针的方法，试剂和试剂盒。

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