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1. 发明授权

US06528648B2 Detecting reagent for double-stranded nucleic acid and double-stranded nucleic acid detecting method 失效
标题翻译：双链核酸检测试剂和双链核酸检测方法
公开(公告)号：US06528648B2
公开(公告)日：2003-03-04
申请号：US10012211
申请日：2001-12-05
申请人： Kazuko Matsumoto , Takahiko Nojima , Hideo Tashiro , Yasumitsu Kondoh , Shigeori Takenaka
发明人： Kazuko Matsumoto , Takahiko Nojima , Hideo Tashiro , Yasumitsu Kondoh , Shigeori Takenaka
IPC分类号： C07D29100
CPC分类号： C09B57/10 , C09B57/08 , C12Q1/6816 , C12Q2563/173
摘要： The present invention provides a novel detecting reagent for double-stranded nucleic acid, and a method of using it to detect double-stranded nucleic acid formed by hybridization with a probe, with absolutely no labeling of the target nucleic acid. The detecting reagent for double-stranded nucleic acid of the invention is characterized by comprising, in the same molecule, a naphthalenediimide group which is intercalatable into double-stranded nucleic acid and a &bgr;-diketone group capable of forming a lanthanoid metal complex.
摘要翻译：本发明提供了一种用于双链核酸的新型检测试剂及其用于检测与探针杂交形成的双链核酸的方法，绝对没有靶核酸的标记。双重检测试剂其特征在于在相同分子中包含可插入双链核酸的萘二酰亚胺基团和能够形成镧系元素金属络合物的β-二酮基团。

2. 发明申请

US20090211907A1 Separation Medium for Biochemical Analysis 审中-公开
标题翻译：分离培养基用于生物化学分析
公开(公告)号：US20090211907A1
公开(公告)日：2009-08-27
申请号：US11884993
申请日：2006-02-24
申请人： Mitsutoshi Masuda , Kazuko Matsumoto , Rika Iwaura , Yoshinori Yamaguchi , Toshimi Shimizu
发明人： Mitsutoshi Masuda , Kazuko Matsumoto , Rika Iwaura , Yoshinori Yamaguchi , Toshimi Shimizu
IPC分类号： B01D57/02 , G01N27/26
CPC分类号： B01D57/02 , C07K1/26 , C07K1/28 , G01N27/44747
摘要： The invention provides a novel packing composition for separation and/or analysis which permits easy capillary replacement; a process for the production of the packing composition; a method for filling a capillary with the packing composition; and electrophoretic methods (such as capillary electrophoresis) with the same. The invention relates to a packing composition for electrophoretic separation and/or analysis which contains a long self-assembly produced by dissolving a low-molecular-weight amphiphilic compound having a hydrophobic moiety and a hydrophilic moiety in water under heating and then cooling the resulting solution; a process for the production of the packing composition; a method of separation and/or analysis with the composition; and so on.
摘要翻译：本发明提供了用于分离和/或分析的新型包装组合物，其允许容易的毛细管置换; 一种生产包装组合物的方法; 用填充组合物填充毛细管的方法; 和电泳方法（如毛细管电泳）相同。本发明涉及一种用于电泳分离和/或分析的包装组合物，其包含通过在加热下将具有疏水部分和亲水部分的低分子量两亲性化合物溶解在水中，然后冷却所得溶液而产生的长自组装 ; 一种生产包装组合物的方法; 用该组合物分离和/或分析的方法; 等等。

3. 发明申请

US20080142730A1 Fluorescence detecting device 审中-公开
标题翻译：荧光检测装置
公开(公告)号：US20080142730A1
公开(公告)日：2008-06-19
申请号：US11979579
申请日：2007-11-06
申请人： Masao Makiuchi , Kazuko Matsumoto
发明人： Masao Makiuchi , Kazuko Matsumoto
IPC分类号： G01N21/64
CPC分类号： G01N21/6408 , G01N21/645 , G01N21/6452 , G01N2021/6484
摘要： To provide a technology of increasing a sensitivity of detecting fluorescence. A fluorescence detecting device includes an excitation light source emitting excitation light that excites a fluorescence-marked measured object, a first optical path via which the excitation light impinges on the fluorescence-marked measured object, a detector detecting fluorescence emitted when the excitation light impinges on the fluorescence-marked measured object, a second optical path via which the fluorescence gets incident on the detector, and a chopper chopping the excitation light passing through the first optical path and the fluorescence passing through the second optical path, and thus controlling a relative relationship between a passage period of the excitation light and a passage period of the fluorescence.
摘要翻译：提供增加检测荧光的灵敏度的技术。荧光检测装置包括：激发光源，其发射激发荧光标记测量对象的激发光;激发光照射在荧光标记的被测物体上的第一光路;检测器，其检测当激发光照射时发射的荧光荧光标记测量对象，荧光入射到检测器上的第二光路，以及切割通过第一光路的激发光和通过第二光路的荧光的斩光器，从而控制相关关系在激发光的通过期间和荧光的通过期间。

4. 发明授权

US5856479A Fluorescent group-containing carbodiimide compound 失效
标题翻译：含荧光素的碳二亚胺化合物
公开(公告)号：US5856479A
公开(公告)日：1999-01-05
申请号：US857536
申请日：1997-05-16
申请人： Osamu Suzuki , Gen Masuda , Namiko Shiohata , Kazuko Matsumoto
发明人： Osamu Suzuki , Gen Masuda , Namiko Shiohata , Kazuko Matsumoto
IPC分类号： C07C267/00 , C07H21/00 , G01N33/58 , C12Q1/68
CPC分类号： C07H21/00 , C07C267/00 , G01N33/582
摘要： Using the fluorescent group-containing carbodiimide compound represented-by the following formula (I) as the label and the like in the nucleic acid detection method, immunoassay, or chemiluminescence assay, labeling can be made efficiently for a short time, a nucleic acid derived from nature can be labelled, and highly sensitive assay is enabled.B--Y.sup.3 --N.dbd.C.dbd.N--Y.sup.2 --W--Y.sup.1 --�A!.sub.n --F(I)wherein F represents a fluorescent group;A represents a moiety selected from the group consisting of --CH.sub.2 --, --NHCO--, --CONH--, --O--, --S--, --NR-- wherein R is an alkyl group, --COO--, --OCO--, --NHSO.sub.2 --, and --SO.sub.2 NH--;n is 0 or 1;W represents a direct bond or a quaternary ammonium group;Y.sup.1, Y.sup.2 and Y.sup.3 each represents an alkylene group which may have a functional group in its main chain; andB represents H or a monovalent organic group which may be the same as or different from that represented by --W--Y.sup.1 --�A!.sub.n --F.
摘要翻译：在核酸检测方法，免疫测定或化学发光测定中使用由下式（I）表示的含荧光基的碳二亚胺化合物作为标记等，可以在短时间内有效地进行标记，得到的核酸自然界可以进行标记，高灵敏度测定可用.B-Y3-N = C = N-Y2-W-Y1- [A] nF（I）其中F表示荧光基团; A表示选自-CH 2 - ， - NHCO - ， - CONH - ， - O - ， - S - ， - NR-的基团，其中R是烷基，-COO - ， - OCO-， - NHSO 2 - 和-SO 2 NH-; n为0或1; W表示直接键或季铵基; Y1，Y2和Y3各自表示在其主链中可具有官能团的亚烷基; B表示H或可以与-W-Y1- [A] n -F表示的相同或不同的一价有机基团。

5. 发明申请

US20060029938A1 Novel method of highly sensitive nucleic acid analysis 审中-公开
标题翻译：高灵敏度核酸分析的新方法
公开(公告)号：US20060029938A1
公开(公告)日：2006-02-09
申请号：US10506240
申请日：2003-03-10
申请人： Kazuko Matsumoto , Jingli Yuan
发明人： Kazuko Matsumoto , Jingli Yuan
IPC分类号： C12Q1/68 , C07F5/00
CPC分类号： C12Q1/6818 , C12Q1/6827 , C12Q2563/137 , C12Q2561/12 , C12Q2561/109
摘要： It is intended to provide a method of highly sensitively analyzing SNPs. More specifically, it is intended to provide an SNP analysis method whereby a large amount of SNPs can be quickly analyzed using a DNA sample in a trace amount. An SNP analysis method by the invader method with the use of a FRET probe having a luminescent dye and a quencher characterized by using, as the luminescent dye in the FRET probe, a rare earth fluorescent complex label made of a rare earth element such as europium or terbium. A composition for controlling fluorescent luminescence which comprises a combination of a specific rare earth fluorescent complex label with a specific fluorescent quencher. A highly sensitive labeling probe characterized by using a rare earth fluorescent complex label as a luminescent dye and a fluorescent quencher label as a quencher in a highly sensitive labeling probe such as a FRET probe and an SNP analysis kit containing the same.
摘要翻译：旨在提供高灵敏度分析SNP的方法。更具体地，旨在提供一种SNP分析方法，其中可以使用微量的DNA样品快速分析大量的SNP。通过使用具有发光染料和猝灭剂的FRET探针的入侵者方法的SNP分析方法，其特征在于使用FRET探针中的发光染料作为稀土元素如铕的稀土荧光复合体标记或铽。用于控制荧光发光的组合物，其包含特定稀土荧光复合物标记与特定荧光猝灭剂的组合。特征在于使用稀土荧光复合体标记作为发光染料的高敏感性标记探针，以及在高灵敏度标记探针如FRET探针和含有该荧光标记的SNP分析试剂盒中使用荧光猝灭剂标记作为猝灭剂。

6. 发明申请

US20050255465A1 Novel fluorescent label compounds 有权
标题翻译：新型荧光标记化合物
公开(公告)号：US20050255465A1
公开(公告)日：2005-11-17
申请号：US10506242
申请日：2003-03-10
申请人： Kazuko Matsumoto , Jingli Yuan , Guilan Wang , Mingqian Tan
发明人： Kazuko Matsumoto , Jingli Yuan , Guilan Wang , Mingqian Tan
IPC分类号： C07D213/36 , C07D213/38 , C07D401/14 , C07D409/14 , C07D491/14 , C07D498/22 , C07F5/00 , C12Q1/68 , G01N33/533
CPC分类号： C07D213/36 , C07D401/14 , G01N33/533
摘要： It is intended to provide novel labeling reagents characterized by having a group capable of binding to a substance to be labeled (for example, a biological substance, a physiologically active substance, etc.), easily forming a complex together with a rare earth ion, the complex being stable in an aqueous solution, and having a sufficient fluorescence intensity and a long fluorescence life time regardless of buffer types; complexes composed of the above labeling reagent with a rare earth ion; fluorescence labels containing the above complex; a fluorescence assay method using the above fluorescent label; etc. Namely, labeling reagents comprising a compound having a 2,2′:6′,2″-tripyridine skeleton or a 2,6-dipyrazolopyridine skeleton and having a group capable of binding to a substance to be labeled (for example, a biological substance, a physiologically active substance, etc.) and a group capable of forming a complex together with a rare earth ion; complexes composed of the above labeling reagent with a rare earth ion; fluorescence labels containing the above complex; a fluorescence labeling method using the above complex as a label; and a fluorescence assay method using the above fluorescent label.
摘要翻译：旨在提供新颖的标记试剂，其特征在于具有能够与待标记的物质结合的基团（例如生物物质，生理活性物质等），容易与稀土离子一起形成络合物，该复合物在水溶液中稳定，并且具有足够的荧光强度和长的荧光寿命，而不管缓冲液类型如何; 由上述标记试剂与稀土离子组成的络合物; 含有上述复合物的荧光标记物; 使用上述荧光标记物的荧光检测方法; 即，包括具有2,2'：6'，2“ - 三吡啶骨架或2,6-二吡唑并吡啶骨架的化合物的标记试剂，并具有能够结合待标记的物质的基团（例如，生物物质，生理活性物质等）和能够与稀土离子一起形成络合物的基团; 由上述标记试剂与稀土离子组成的络合物; 含有上述复合物的荧光标记物; 使用上述复合物作为标签的荧光标记方法; 和使用上述荧光标记物的荧光测定方法。

7. 发明授权

US06777181B2 Method for separating and collecting nucleic acids 失效
标题翻译：分离和收集核酸的方法
公开(公告)号：US06777181B2
公开(公告)日：2004-08-17
申请号：US09771439
申请日：2001-01-26
申请人： Kazuko Matsumoto
发明人： Kazuko Matsumoto
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , C12Q2537/157 , C12Q2565/501
摘要： A method for separating and collecting nucleic acids, which comprises: a step of bringing a sample nucleic acid solution into contact with a nucleic acid-immobilized substrate comprising a substrate and two or more kinds of single-stranded nucleic acids separately immobilized on the substrate, to allow hybridization of the immobilized single-stranded nucleic acids and single-stranded nucleic acids complementary to the immobilized single-stranded nucleic acids, and a step of separating the hybridized single-stranded nucleic acids according to immobilized portions of the immobilized nucleic acids, to collect the hybridized single-stranded nucleic acids without disassembling the nucleic acid-immobilized substrate.
摘要翻译：一种分离和收集核酸的方法，其包括：使样品核酸溶液与包含底物的核酸固定化底物和分别固定在所述底物上的两种或更多种单链核酸接触的步骤，以允许固定化的单链核酸和与固定的单链核酸互补的单链核酸的杂交，以及根据固定的核酸的固定化部分分离杂交的单链核酸的步骤，以收集杂交的单链核酸，而不拆分核酸固定的底物。

8. 发明授权

US07255998B1 High sensitivity immunoassay method 失效
标题翻译：高灵敏度免疫测定法
公开(公告)号：US07255998B1
公开(公告)日：2007-08-14
申请号：US10089776
申请日：2000-09-28
申请人： Kei Tashiro , Tasuku Honjo , Masaya Ikegawa , Kazuko Matsumoto
发明人： Kei Tashiro , Tasuku Honjo , Masaya Ikegawa , Kazuko Matsumoto
IPC分类号： G01N33/53 , G01N33/532 , G01N33/533 , G01N33/24 , G01N21/76 , G01N33/547 , C07K1/10
CPC分类号： G01N33/582 , G01N33/6863 , G01N2458/40 , Y10S435/968 , Y10S435/97 , Y10S435/975
摘要： A method for detecting a cytokine in a biological fluid sample with a high sensitivity is provided. A time-resolved fluoroimmunoassay (TR-FIA) method including a step of forming on a solid phase a composite in which a cytokine is captured and which includes a fluorescent structural portion which has been complexed with a lanthanoid metal ion, and measuring fluorescence of the fluorescent structural portion. The composite is formed of a structure in which (a) a first antibody including a portion bound to a solid phase and a region bindable to a cytokine; (b) the cytokine; (c) a second antibody including a region bindable to the cytokine and a portion to which biotin is bound; (d) a conjugate including streptoavidin or avidin and a fluorescent structural portion capable of being complexed with a lanthanoid metal ion; and (e) the lanthanoid metal ion are bound. The fluorescent structural portion is represented by General Formula (I): R—Ar—C(═O)—CH2—C(═O)—CnF2n—X.
摘要翻译：提供了一种以高灵敏度检测生物流体样品中的细胞因子的方法。一种时间分辨荧光免疫测定（TR-FIA）方法，包括在固相上形成其中捕获细胞因子的复合物并包括与镧系元素金属离子络合的荧光结构部分的步骤，以及测量荧光结构部分。所述复合物由以下结构形成：其中（a）包含与固相结合的部分和与细胞因子结合的区域的第一抗体; （b）细胞因子; （c）包含与细胞因子结合的区域和生物素结合部分的第二抗体; （d）包含链霉亲和素或抗生物素蛋白的缀合物和能够与镧系金属离子络合的荧光结构部分; 和（e）镧系金属离子结合。荧光结构部分由通式（I）表示：R-Ar-C（-O）-CH 2 -C（-O）-C N SUB> 2n -X。

9. 发明申请

US20070026407A1 Novel fine fluorescent particle 审中-公开
标题翻译：新型细荧光颗粒
公开(公告)号：US20070026407A1
公开(公告)日：2007-02-01
申请号：US10570768
申请日：2004-09-08
申请人： Kazuko Matsumoto , Takuya Nishioka
发明人： Kazuko Matsumoto , Takuya Nishioka
IPC分类号： C12Q1/68 , C07F5/00 , C07D487/22
CPC分类号： G01N33/533 , C09K11/02 , C09K11/06 , C09K2211/1007 , C09K2211/1029 , C09K2211/1044 , C09K2211/182 , G01N33/54313
摘要： Novel silica particles having bondable functional groups on the surface thereof and containing a fluorescent rare-earth complex characterized by being uninfluenced by the surrounding environment including a buffer and a substance to be labeled. The silica particles containing a fluorescent rare-earth complex specifically are ones which comprise silica particles containing a fluorescent rare-earth complex selectively in an inner layer thereof and the surface of which consists substantially of silica. Also provided are: a fluorescent labeling agent comprising the silica particles; a method of fluorescent labeling which comprises using the silica particles as a labeling agent; and a method for fluorescent determination and a reagent for fluorescent determination which employ the fluorescent labeling agent.
摘要翻译：新型二氧化硅颗粒在其表面上具有可键合的官能团并含有荧光稀土络合物，其特征在于不受周围环境的影响，包括缓冲液和待标记的物质。含有荧光稀土配合物的二氧化硅粒子具体是选自内层的含有荧光稀土配合物的二氧化硅粒子，其表面基本上由二氧化硅构成。还提供：包含二氧化硅颗粒的荧光标记剂; 包括使用二氧化硅颗粒作为标记试剂的荧光标记方法; 以及采用荧光标记剂的荧光测定方法和荧光测定用试剂。

10. 发明授权

US06455684B1 Insitu assay of substance in biological sample using labeled probe 失效
标题翻译：使用标记探针对生物样品中的物质进行原位测定
公开(公告)号：US06455684B1
公开(公告)日：2002-09-24
申请号：US09967361
申请日：2001-09-28
申请人： Kazuhiro Matsui , Katsunori Ikeda , Shinichi Teshima , Yoshihisa Kawamura , Kazuko Matsumoto
发明人： Kazuhiro Matsui , Katsunori Ikeda , Shinichi Teshima , Yoshihisa Kawamura , Kazuko Matsumoto
IPC分类号： C07H2102
CPC分类号： G01N33/582 , G01N2458/40
摘要： A method for analyzing an objective substance, comprising reacting a labeled probe with an objective substance on a biological sample, said probe comprising a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or —COCH2COCnF2n+1 and n is an integer of 1-6, which is bonded to a probe selected from the group consisting of nucleic acid, nucleic acid binding protein, low molecular ligand and receptor for ligand (except antibody) to give a fluorescent complex, reacting the complex with an objective substance on a biological sample and assaying fluorescence of the resultant fluorescent complex, a labeled nucleic acid probe and a labeled nucleotide. According to the method of the present invention, defects such as hindrance of fluorescence due to contaminant substance, low sensitivity and the like can be resolved, thereby enabling analysis on a tissue.
摘要翻译：一种用于分析目标物质的方法，包括使标记的探针与生物样品上的目标物质反应，所述探针包含式（I）的标记物质：其中A1为芳族基团，R1为氢或-COCH2COCnF2n + 1和n是1-6的整数，其与选自核酸，核酸结合蛋白，低分子配体和配体（抗体除外）的受体的探针结合，得到荧光复合物，使与生物样品上的目标物质复合，并测定所得荧光复合物，标记的核酸探针和标记的核苷酸的荧光。根据本发明的方法，可以解决诸如由于污染物质引起的荧光阻碍，低灵敏度等的缺陷，从而能够对组织进行分析。

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