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1. 发明授权

US07273759B2 Plate alignment and sample transfer indicia for a multiwell multiplate stack and method for processing biological/chemical samples using the same 失效
标题翻译：多孔堆叠的板对准和样品转移标记以及使用其进行生物/化学样品处理的方法
公开(公告)号：US07273759B2
公开(公告)日：2007-09-25
申请号：US10236953
申请日：2002-09-09
申请人： Jonathan L. Coffman , Hong Lu , Ernest J. Wodds, III
发明人： Jonathan L. Coffman , Hong Lu , Ernest J. Wodds, III
IPC分类号： G01N1/10
CPC分类号： B01L3/50255 , B01L3/5025 , G01N1/405 , G01N35/1095 , G01N2001/388 , G01N2035/00237 , G01N2035/00277 , G01N2035/00485 , Y10T436/2575
摘要： Device and method for processing samples are provided. A first multiwell plate is stacked atop a second multiwell plate. The first multiwell plate has x wells arranged in an array, each well is capable of receiving a sample and has an outlet. The second multiwell plate has y wells arranged in an array, each well being capable of receiving a separate sample. y>x. The outlets of the first multiwell plate register with inlets of a subset of wells of the second multiwell plate when the first plate is stacked atop the second plate. Also provided are a mechanism for aligning the plates and transfer indicia for tracking transfer of sample from the first to the second plate. A mechanism for sealing the first plate to the second plate is provided so that samples can be directly transferred between the plates without cross-contamination occurring in open wells of the second plate.
摘要翻译：提供了处理样品的装置和方法。第一多孔板堆叠在第二多孔板上。第一个多孔板具有排列成阵列的x个孔，每个孔能够接收样品并具有出口。第二个多孔板具有以阵列排列的y个孔，每个孔能够接收单独的样品。 y> x。当第一板堆叠在第二板顶上时，第一多孔板的出口对准第二多孔板的孔子集的入口。还提供了用于对准板和传送标记以跟踪样品从第一板到第二板的转移的机构。提供了将第一板密封到第二板的机构，使得样品可以在板之间直接传递，而不会在第二板的开放井中发生交叉污染。

2. 发明授权

US06464942B2 Plate alignment and sample transfer indicia for a multiwell multiplate stack and method for processing biological/chemical samples using the same 失效
标题翻译：多孔堆叠的板对准和样品转移标记以及使用其进行生物/化学样品处理的方法
公开(公告)号：US06464942B2
公开(公告)日：2002-10-15
申请号：US09316850
申请日：1999-05-21
申请人： Jonathan L. Coffman , Hong Lu , Ernest J. Woods, III
发明人： Jonathan L. Coffman , Hong Lu , Ernest J. Woods, III
IPC分类号： B01L300
CPC分类号： B01L3/50255 , B01L3/5025 , G01N1/405 , G01N35/1095 , G01N2001/388 , G01N2035/00237 , G01N2035/00277 , G01N2035/00485 , Y10T436/2575
摘要： Device and method for processing biological, biochemical or chemical samples are provided wherein a first multiwell plate is stacked atop a second multiwell plate. The first multiwell plate has x wells arranged in a regular array, each capable of receiving a separate sample. Each well of the x wells has an outlet at a lower surface of the first plate. The second multiwell plate has y wells arranged in a regular array, each well of the y wells being capable of receiving a separate sample, and wherein y>x. The outlets at the lower surface of the first multiwell plate are arrayed to register with corresponding inlets of a subset of the y wells of the second multiwell plate when the first plate is stacked atop the second plate. A mechanism for aligning the first plate to the second plate is provided, as well as transfer indicia for tracking transfer of sample from the first plate to the second plate. Also, a mechanism for sealing,the first plate to the second plate is provided so that samples can be directly transferred between the first plate and the second plate without cross-contamination occurring in open wells of the second plate.
摘要翻译：提供了用于处理生物，生化或化学样品的装置和方法，其中第一多孔板堆叠在第二多孔板上。第一个多孔板具有排列成规则阵列的x个孔，每个能够接收单独的样品。 x孔的每个孔在第一板的下表面具有出口。第二多孔板具有以规则阵列排列的y孔，y孔的每个孔能够接收单独的样品，并且其中y> x。第一多孔板的下表面处的出口排列成与第二多孔板的y阱的子集的相应入口对准，当第一板堆叠在第二板的顶部时。提供了用于将第一板与第二板对准的机构，以及用于跟踪将样品从第一板传递到第二板的标记。此外，设置用于密封第一板到第二板的机构，使得样品可以在第一板和第二板之间直接传递，而不会在第二板的开放井中发生交叉污染。

3. 发明授权

US07223848B2 Methods for purifying Fc-containing proteins 失效
标题翻译：用于纯化含Fc蛋白质的方法
公开(公告)号：US07223848B2
公开(公告)日：2007-05-29
申请号：US10163853
申请日：2002-06-05
申请人： Jonathan L. Coffman , William B. Foster , Shujun Sun
发明人： Jonathan L. Coffman , William B. Foster , Shujun Sun
IPC分类号： C07K1/18 , C07K1/20 , C07K1/22
CPC分类号： C07K1/36 , B01D15/20 , B01D15/327 , C07K1/22 , C07K14/31 , C07K2319/30 , Y10S436/828
摘要： The present invention provides a process for dissociating Fc-containing molecules from complexes of Protein A/Fc-containing molecules or mixtures containing Fc-containing molecules and Protein A. The association, e.g., by hydrophobic interactions, between the Fc-containing molecules and Protein A can be reduced or inhibited by raising the pH of dissociation. The pH of dissociation can be raised by addition of agents capable of inhibiting hydrophobic interactions, including buffers containing arginine and/or ethylene glycol, to the mixture, either prior to adding the mixture to the column chromatography substrate, after adding the mixture to the column chromatography substrate, or both prior to and after adding the mixture to the column chromatography substrate. Separation of Fc-containing molecules from Protein A can be performed on a number of different column chromatographic substrates, including column chromatographic substrates contained in Q columns (Q column chromatography substrate), HIC columns (hydrophobic interaction column chromatography substrate), and IMAC columns (metal chelate column chromatography substrate).
摘要翻译：本发明提供了一种从包含含蛋白A / Fc的分子或含有Fc分子和蛋白质A的混合物的复合物中解离含Fc分子的方法。在含Fc分子和蛋白质之间的关联，例如通过疏水相互作用可以通过提高解离的pH来降低或抑制A。在将混合物加入到柱中之前，可以通过加入能够抑制疏水相互作用的试剂（包括含有精氨酸和/或乙二醇的缓冲液）到混合物中来提高解离的pH值。色谱底物，或在将混合物加入柱色谱底物之前和之后两者。含有Fc的分子与蛋白A的分离可以在许多不同的色谱层析基质上进行，包括Q柱（Q柱色谱底物），HIC柱（疏水相互作用柱色谱底物）和IMAC色谱柱金属螯合柱色谱法）。

4. 发明授权

US06933370B2 Methods for purifying highly anionic proteins 有权
标题翻译：纯化高度阴离子蛋白的方法
公开(公告)号：US06933370B2
公开(公告)日：2005-08-23
申请号：US09819157
申请日：2001-03-27
申请人： Jonathan L. Coffman , William Barry Foster , Bonnie J. Germain , Shujun Sun , Jeffrey J. Robinson
发明人： Jonathan L. Coffman , William Barry Foster , Bonnie J. Germain , Shujun Sun , Jeffrey J. Robinson
IPC分类号： C07K14/00 , C07K1/14 , C07K1/18 , C07K1/20 , C07K14/705 , C07K19/00 , A23J1/00 , C07K1/00
CPC分类号： C07K1/20 , C07K1/18 , C07K1/36 , C07K14/70596 , C07K2319/00 , C07K2319/30
摘要： The present invention provides a process for isolating and purifying highly anionic target proteins, for example, sulfated proteins. Sulfated proteins have five (5) or more, sulfations. In a preferred embodiment, the sulfated protein has six (6) sulfations, e.g., six sulfations on N-terminal tyrosine residues, as embodied in PSGL-1 (P-selectin glycoprotein ligand).
摘要翻译：本发明提供了用于分离和纯化高度阴离子的靶蛋白，例如硫酸化蛋白质的方法。硫酸化蛋白质有五（5）或更多硫酸盐。在优选的实施方案中，如在PSGL-1（P-选择素糖蛋白配体）中体现的，硫酸化蛋白质具有六（6）个硫酸盐，例如在N末端酪氨酸残基上的六个硫酸盐。

5. 发明授权

US5981736A One step device and process for concentration and purification of biological molecules 失效
标题翻译：生物分子浓缩和纯化的一步装置和方法
公开(公告)号：US5981736A
公开(公告)日：1999-11-09
申请号：US104795
申请日：1998-06-25
申请人： Jonathan L. Coffman
发明人： Jonathan L. Coffman
IPC分类号： G01N30/06 , B01D15/08 , C12M1/00 , C12N15/10 , C12Q1/68 , G01N30/02 , G01N30/60 , C07H21/04
CPC分类号： C12N15/1006 , B01D15/1871 , B01D15/34 , B01D15/26 , G01N30/02
摘要： A device for one step purification of a desired biological molecule from a sample, wherein the device comprises a housing loaded with an adsorptive media of a known volume on top of a size exclusion media of a known volume, and a method of purifying biological molecules using the same.
摘要翻译：一种用于从样品中一步纯化所需生物分子的装置，其中所述装置包括在已知体积的尺寸排阻介质顶部装载已知体积的吸附介质的壳体，以及使用一样。

6. 发明授权

US5906747A Separation of molecules from dilute solutions using composite chromatography media having high dynamic sorptive capacity at high flow rates 失效
标题翻译：使用在高流速下具有高动态吸附容量的复合色谱介质从稀溶液中分离分子
公开(公告)号：US5906747A
公开(公告)日：1999-05-25
申请号：US556313
申请日：1995-11-13
申请人： Jonathan L. Coffman , Pierre Girot , Egisto Boschetti
发明人： Jonathan L. Coffman , Pierre Girot , Egisto Boschetti
IPC分类号： B01D15/08 , C07K1/18
CPC分类号： B01D15/08 , B01D15/361 , C07K1/18
摘要： This invention relates generally to modified porous solid chromatographic media and processes for the preparation and use of same. In particular, chromatographic media of porous mineral oxide, polymeric, or polymer-coated mineral oxide supports are disclosed which are characterized by a reversible high sorptive capacity and high intraparticle mass transfer rates. In order to prevent non-specific adsorption of or interaction with biomolecules, these supports may be passivated by use of a passivation mixture comprising a main monomer, a passivating monomer, and a crosslinking agent, which mixture upon polymerization results in substantial elimination of the undesirable non-specific interaction with biomolecules.
摘要翻译：本发明一般涉及改性多孔固体色谱介质及其制备和使用方法。特别地，公开了多孔矿物氧化物，聚合物或聚合物涂覆的矿物氧化物载体的色谱介质，其特征在于可逆的高吸附能力和高的颗粒内传质速率。为了防止非特异性吸附或与生物分子的相互作用，这些载体可以通过使用包含主单体，钝化单体和交联剂的钝化混合物进行钝化，该聚合物上的该混合物导致基本上不需要的与生物分子的非特异性相互作用。

7. 发明授权

US07855280B2 Separation methods 有权
标题翻译：分离方法
公开(公告)号：US07855280B2
公开(公告)日：2010-12-21
申请号：US11518532
申请日：2006-09-08
申请人： Jonathan L. Coffman , Russell I. Shpritzer , Steven M. Vicik
发明人： Jonathan L. Coffman , Russell I. Shpritzer , Steven M. Vicik
IPC分类号： A23J3/20 , C07K14/00
CPC分类号： C07K1/30 , C07K1/36 , C07K16/22
摘要： Separation methods, for example, to isolate a recombinant protein, are disclosed. In some implementations, a method includes forming a solid containing a first cation and a first anion in a medium containing a protein, and separating the solid from the protein.
摘要翻译：公开了分离方法，例如分离重组蛋白。在一些实施方案中，一种方法包括在含有蛋白质的培养基中形成含有第一阳离子和第一阴离子的固体，并将该固体与蛋白质分离。

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