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1. 发明申请

US20080108127A1 Plant Activation of Insect Toxin 失效
公开(公告)号：US20080108127A1
公开(公告)日：2008-05-08
申请号：US11950509
申请日：2007-12-05
申请人： Andre Abad , Ronald Flannagan , Rafael Hermann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , James Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald Flannagan , Rafael Hermann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , James Wong , Cao-Guo Yu
IPC分类号： C07K16/00 , C12N9/50
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

2. 发明申请

US20070130641A1 Methods and compositions for expressing an herbicide-tolerant polynucleotide 有权
标题翻译：用于表达除草剂耐受性多核苷酸的方法和组合物
公开(公告)号：US20070130641A1
公开(公告)日：2007-06-07
申请号：US11507997
申请日：2006-08-22
申请人： Billy McCutchen , Christine Hazel , Donglong Liu , Albert Lu , Wayne Mehre , Paul Olson , James Wong
发明人： Billy McCutchen , Christine Hazel , Donglong Liu , Albert Lu , Wayne Mehre , Paul Olson , James Wong
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8274 , C12N9/1092 , C12N9/88 , C12N15/8205 , C12N15/8209 , C12N15/8216 , C12N15/8271 , C12N15/8275 , C12N15/8278 , C12N15/8286 , Y02A40/162
摘要： Methods and compositions are provided related to improved plants that are tolerant to more than one herbicide. Particularly, the invention provides plants that are tolerant of glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. The glyphosate/ALS inhibitor-tolerant plants comprise a polynucleotide that encodes a polypeptide that confers tolerance to glyphosate and a polynucleotide that encodes an ALS inhibitor-tolerant polypeptide. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods to control weeds, improve plant yield, and increase transformation efficiencies are provided.
摘要翻译：提供了与多于一种除草剂耐受的改良植物相关的方法和组合物。特别地，本发明提供了耐草甘膦并且耐受至少一种ALS抑制剂的植物及其使用方法。草甘膦/ ALS抑制剂耐受性植物包含编码赋予对草甘膦的耐受性的多肽的多核苷酸和编码ALS抑制剂耐受性多肽的多核苷酸。在具体实施方案中，本发明的植物表达GAT多肽和HRA多肽。提供了控制杂草，提高植物产量和提高转化效率的方法。

3. 发明申请

US20050273881A1 Defensin polynucleotides and methods of use 有权
标题翻译：防御素多核苷酸和使用方法
公开(公告)号：US20050273881A1
公开(公告)日：2005-12-08
申请号：US11123896
申请日：2005-05-06
申请人： Carl Simmons , Pedro Acevedo , Leslie Harvell , Rebecca Cahoon , Billy McCutchen , Albert Lu , Rafael Herrmann , James Wong
发明人： Carl Simmons , Pedro Acevedo , Leslie Harvell , Rebecca Cahoon , Billy McCutchen , Albert Lu , Rafael Herrmann , James Wong
IPC分类号： C07K14/415 , C07K14/47 , C12N15/82 , A01H1/00 , C07H21/04 , C12N5/04
CPC分类号： C07K14/4723 , C07K14/415 , C12N15/8279
摘要： Methods and compositions for modulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节发展和防御反应的方法和组成。提供编码防御素蛋白质的核苷酸序列。序列可用于表达盒用于调节发育，发育途径和防御反应。还提供了转化的植物，植物细胞，组织和种子。

4. 发明申请

US20050198706A1 Lipases and methods of use 有权
标题翻译：脂肪酶和使用方法
公开(公告)号：US20050198706A1
公开(公告)日：2005-09-08
申请号：US11061233
申请日：2005-02-18
申请人： Billy McCutchen , Andre Abad , James Wong , Cao Yu
发明人： Billy McCutchen , Andre Abad , James Wong , Cao Yu
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8286 , C12N9/20 , Y02A40/162
摘要： Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.
摘要翻译：提供了通过将非植物脂肪酶引入植物来产生和增强植物中抗虫性的方法。提供具有增强的抗虫性的植物和植物种子。还提供了可用于本发明实践的编码杀虫脂肪酶和杀虫脂肪酶基因产物的DNA序列。本发明的组合物和方法可用于各种用于防治害虫的农业系统，包括传播昆虫抗性作物谱系，并将这些杀虫脂肪酶靶向特别容易受到侵染的植物器官的表达，如根和叶。

5. 发明申请

US20050166284A1 Plant activation of insect toxin 有权
标题翻译：植物激活昆虫毒素
公开(公告)号：US20050166284A1
公开(公告)日：2005-07-28
申请号：US11021115
申请日：2004-12-22
申请人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , James Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , James Wong , Cao-Guo Yu
IPC分类号： A01H1/00 , A01H5/00 , C07K14/325 , C12N9/64 , C12N15/82
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

6. 发明申请

US20050042717A1 Scorpion toxins 有权
标题翻译：蝎毒素
公开(公告)号：US20050042717A1
公开(公告)日：2005-02-24
申请号：US10614934
申请日：2003-07-08
申请人： Rafael Herrmann , James Presnail , Albert Lu , James Wong , Jian-Ming Lee
发明人： Rafael Herrmann , James Presnail , Albert Lu , James Wong , Jian-Ming Lee
IPC分类号： C07K14/435 , C12N1/15 , C12N1/21 , C12N15/12 , C12N15/82 , C07K14/705 , C07H21/04 , C12N7/00 , C12N1/18 , C12N5/04
CPC分类号： C12N15/8286 , C07K14/43522 , Y02A40/162
摘要： This invention relates to an isolated nucleic acid fragment encoding scorpion toxins that are K-channel agonists. The invention also relates to the construction of a chimeric gene encoding all or a portion of the K-channel agonists, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the K-channel agonists in a transformed host cell.
摘要翻译：本发明涉及编码作为K-通道激动剂的蝎毒素的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分K-通道激动剂的嵌合基因，其中嵌合基因的表达导致转化的K通道激动剂水平的改变宿主细胞。

7. 发明授权

US06543123B1 Process for making constrained filament niobium-based superconductor composite 有权
标题翻译：制造受限长丝铌基超导体复合材料的工艺
公开(公告)号：US06543123B1
公开(公告)日：2003-04-08
申请号：US09532362
申请日：2000-03-21
申请人： James Wong
发明人： James Wong
IPC分类号： H01L3924
CPC分类号： G06Q10/02 , C23C2/02 , C23C2/08 , C23C2/28 , H01L39/2409 , Y10T29/49014
摘要： A niobium-based superconductor is manufactured by establishing multiple niobium components in a billet of a ductile metal, working the composite billet through a series of reduction steps to form the niobium components into elongated elements, each niobium element having a thickness on the order of 1 to 25 microns, surrounding the billet prior to the last reduction step with a porous confining layer of an acid resistant metal, immersing the confined billet in an acid to remove the ductile metal from between the niobium elements while the niobium elements remain confined by said porous layer, exposing the confined mass of niobium elements to a material capable of reacting with Nb to form a superconductor.
摘要翻译：铌基超导体通过在延性金属的坯料中建立多个铌组分来制造，通过一系列还原步骤来加工复合坯料，以将铌组分形成为细长元件，每个铌元素的厚度大约为1 至25微米，在最后一次还原步骤之前，用耐酸金属的多孔限制层围绕坯料，将受限制的坯料浸入酸中以从铌元素之间移除延性金属，同时铌元素保持被所述多孔层，将约束的铌元素暴露于能够与Nb反应以形成超导体的材料。

8. 发明授权

US5869196A Constrained filament electrolytic anode and process of fabrication 失效
标题翻译：约束长丝电解阳极及其制造工艺
公开(公告)号：US5869196A
公开(公告)日：1999-02-09
申请号：US931145
申请日：1997-09-16
申请人： James Wong , Mark K. Rudziak , Terence Wong
发明人： James Wong , Mark K. Rudziak , Terence Wong
IPC分类号： B21C23/00 , B21C1/00 , B21C37/04 , B32B20060101 , B32B5/18 , B32B15/00 , H01G9/04 , H01G9/042 , H01G9/052
CPC分类号： H01G9/042 , B21C37/047 , Y10T29/49002 , Y10T428/12479 , Y10T428/12806 , Y10T428/12819
摘要： Porous metal compacts suitable for use as electrodes are formed through the reduction of a metal billet consisting of multiple filaments of an appropriate valve metal, preferably tantalum, contained within, and spaced apart by, a ductile metal, preferably copper. The filaments are elongated and substantially parallel within the billet. The array of valve metal filaments within the billet is surrounded by a continuous layer of valve metal. This metal is preferably, but not necessarily, the same as that which forms the filaments. The valve metal layer preferably completely surrounds the filament array circumferentially and runs the full length of the filaments. The layer is separated from the array by the same ductile metal that serves to separate the filaments from each other. This same ductile metal forms the surface of the billet, preventing exposure of the valve metal layer. The billet is reduced by conventional means, such as extrusion and wire drawing, the composite product is cut into short lengths, and the ductile metal separating the valve metal components of the composite is removed, preferably by leaching in mineral acids. The filaments are constrained within the valve metal tube, making handling during subsequent capacitor manufacture much less difficult than if the filaments were allowed to move freely.
摘要翻译：适合用作电极的多孔金属压块通过还原金属坯料而形成，所述金属坯料由包含在延性金属（优选铜）中并由间隔开的适当的阀金属，优选钽构成。长丝细长并在坯料内基本平行。钢坯内的阀金属细丝阵列被一个连续的金属层包围。该金属优选但不一定与形成长丝的金属相同。阀金属层优选地沿圆周方向完全围绕细丝阵列，并延伸长丝的全长。该层通过相同的延展性金属与阵列分离，其用于将长丝彼此分离。该相同的延性金属形成坯料的表面，防止阀金属层的暴露。通过常规方式，例如挤出和拉丝，将复合材料切割成短长度，并且分离复合材料的阀金属组分的延性金属，优选通过在无机酸中浸出而被除去。长丝被限制在阀金属管内，使得随后的电容器制造期间的处理比如果允许细丝自由移动困难得多。

9. 发明授权

US5223348A APC orientation superconductor and process of manufacture 失效
标题翻译： APC取向超导体和制造工艺
公开(公告)号：US5223348A
公开(公告)日：1993-06-29
申请号：US703174
申请日：1991-05-20
申请人： James Wong , Mark K. Rudziak
发明人： James Wong , Mark K. Rudziak
IPC分类号： H01L39/24
CPC分类号： H01L39/2406 , Y10S428/93 , Y10S505/814 , Y10S505/918 , Y10S505/921 , Y10T29/49014 , Y10T428/12632 , Y10T428/12806 , Y10T428/12812
摘要： Artifical pinning centers are provided in normal metal layers adjacent to a type II superconductor layer produced by reacting two normal metal layers. The transverse thicknesses of the final superconductor and normal metal layers are less than about 1000 A.degree.. Planar layers of metal which are to provide a multilayer wire whose layers are parallel. A plurality of the multilayer wires are combined to produce a multifilament superconductor, the layers in all the individual wires being parallel to each other.
摘要翻译：在与通过两个正常金属层反应制造的II型超导体层相邻的正常金属层中提供人造钉扎中心。最终超导体和正常金属层的横向厚度小于约1000A。平面的金属层，它们要提供一层层叠的多层导线。多个多层导线组合以产生复丝超导体，所有各个线中的层彼此平行。

10. 发明授权

US5158620A Superconductor and process of manufacture 失效
标题翻译：超导体和制造工艺
公开(公告)号：US5158620A
公开(公告)日：1992-10-27
申请号：US480236
申请日：1990-02-15
申请人： James Wong , Mark K. Rudziak , Donald W. Capone, II
发明人： James Wong , Mark K. Rudziak , Donald W. Capone, II
IPC分类号： B21C23/22 , B21C37/04 , C22F1/18 , H01J7/18 , H01L39/24
CPC分类号： H01L39/2406 , B21C23/22 , B21C33/002 , B21C37/042 , B21C37/045 , C22F1/18 , C22F1/183 , C22F1/186 , H01J7/183 , Y10S505/918 , Y10S505/921 , Y10T29/49014
摘要： In the present invention, a superconductor is produced by the steps of wrapping a plurality of layers of metal sheets around a support to form a composite structure. The sheets are pure transition metals-13 niobium, titanium, zirconium, or vanadium, for example--alternate sheets being formed of different transition metals. The support may be composed of any ductile metal. The resulting composite structure is mechanically reduced sufficiently so that each transition metal sheet is less than 1000 .ANG. thick. In the course of reduction, the composite is subjected to sufficient temperatures for sufficient times such that the transition metal layers are partially reacted to form a ductile superconducting material between the transition metal layers. Approximately one half by volume of the transition metal layers remain unreacted. These unreacted layers afford efficient flux pinning within the composite when the layers are reduced to the
摘要翻译：在本发明中，通过以下步骤制造超导体，所述步骤是将多层金属薄片缠绕在支撑体上以形成复合结构。这些片材是纯过渡金属-13铌，钛，锆或钒，例如由不同的过渡金属形成的交替片材。支撑件可以由任何延性金属构成。所得到的复合结构被充分地机械地还原，使得每个过渡金属板的厚度小于1000。在还原过程中，复合材料经受足够的温度足够的时间，使得过渡金属层部分地反应以在过渡金属层之间形成延性超导材料。约一半体积的过渡金属层保持未反应。当层减少到<1000安格姆最终尺寸时，这些未反应层在复合材料内提供有效的磁通钉扎。

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