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    • 7. 发明申请
    • Insect-specific protease recognition sequences
    • 昆虫特异性蛋白酶识别序列
    • US20060288448A1
    • 2006-12-21
    • US11448266
    • 2006-06-07
    • Andre AbadGary ChunRonald FlannaganRafael HerrmannAlbert LuJanet RiceEric Schepers
    • Andre AbadGary ChunRonald FlannaganRafael HerrmannAlbert LuJanet RiceEric Schepers
    • A01H1/00C07H21/04C12N15/82C12N5/04
    • C12N15/8286A01N63/02C07K14/325C12N9/6472Y02A40/162
    • Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
    • 提供了保护植物免受害虫害虫的组合物和方法。 提供编码昆虫原毒素或昆虫毒素的核酸分子,其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。 通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。 在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素,其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。 提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。 本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂,表达盒,以及转化的植物,植物细胞和种子。 昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。