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1. 发明申请

US20050166284A1 Plant activation of insect toxin 有权
标题翻译：植物激活昆虫毒素
公开(公告)号：US20050166284A1
公开(公告)日：2005-07-28
申请号：US11021115
申请日：2004-12-22
申请人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , James Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , James Wong , Cao-Guo Yu
IPC分类号： A01H1/00 , A01H5/00 , C07K14/325 , C12N9/64 , C12N15/82
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。特别地，提供了编码昆虫原毒素的核酸序列，其被修饰为包含对植物蛋白酶或昆虫肠道蛋白酶敏感的至少一个蛋白水解活化位点。通过蛋白酶在蛋白水解活化位点切割修饰的原毒素会产生活性昆虫毒素。提供了使用修饰的昆虫原毒素核酸序列及其编码的多肽保护植物免受害虫的方法。本发明的具体实施方案还提供了改良的昆虫原毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。

2. 发明申请

US20050261483A1 Genes encoding proteins with pesticidal activity 审中-公开
公开(公告)号：US20050261483A1
公开(公告)日：2005-11-24
申请号：US11058727
申请日：2005-02-15
申请人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Theodore Kahn , Albert Lu , Billy McCutchen , James Presnail , James Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Theodore Kahn , Albert Lu , Billy McCutchen , James Presnail , James Wong , Cao-Guo Yu
IPC分类号： C07K14/325 , C12N15/32 , C12N15/82 , G06F19/00 , C07K14/195
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

3. 发明申请

US20050261188A1 Genes encoding proteins with pesticidal activity 有权
标题翻译：编码具有杀虫活性的蛋白质的基因
公开(公告)号：US20050261188A1
公开(公告)日：2005-11-24
申请号：US11108389
申请日：2005-04-18
申请人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Theodore Kahn , Albert Lu , Billy McCutchen , James Presnail , James Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Theodore Kahn , Albert Lu , Billy McCutchen , James Presnail , James Wong , Cao-Guo Yu
IPC分类号： C07K14/325 , C12N15/32 , C12N15/82 , G06F19/00 , A01N63/00
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫害虫的δ-内毒素的苏云金芽孢杆菌菌株获得的核酸及其变体和片段。本发明进一步提供已被修饰以编码杀虫多肽的诱变核酸，例如具有改善的杀虫活性和/或改变的有害生物特异性的内毒素。本发明的具体实施方案提供编码可能被优化的杀虫蛋白质的分离的核酸，以及包含本发明的核酸的农药组合物，表达盒和转化的微生物和植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

4. 发明申请

US20080108127A1 Plant Activation of Insect Toxin 失效
公开(公告)号：US20080108127A1
公开(公告)日：2008-05-08
申请号：US11950509
申请日：2007-12-05
申请人： Andre Abad , Ronald Flannagan , Rafael Hermann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , James Wong , Cao-Guo Yu
发明人： Andre Abad , Ronald Flannagan , Rafael Hermann , Albert Lu , Billy McCutchen , James Presnail , Janet Rice , James Wong , Cao-Guo Yu
IPC分类号： C07K16/00 , C12N9/50
CPC分类号： C12N15/8286 , C12N9/641 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

5. 发明申请

US20060021096A1 Genes encoding proteins with pesticidal activity 有权
标题翻译：编码具有杀虫活性的蛋白质的基因
公开(公告)号：US20060021096A1
公开(公告)日：2006-01-26
申请号：US11224624
申请日：2005-09-12
申请人： Andre Abad , Hua Dong , Rafael Herrmann , Albert Lu , Billy McCutchen , Janet Rice , Eric Schepers , James Wong
发明人： Andre Abad , Hua Dong , Rafael Herrmann , Albert Lu , Billy McCutchen , Janet Rice , Eric Schepers , James Wong
IPC分类号： A01H5/00 , A01H1/00 , C07K14/415
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。本发明的具体实施方案提供杀虫多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。这些组合物可用于防治害虫，特别是植物害虫的方法。还提供了新型植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

6. 发明申请

US20060236424A1 Methods and compositions for designing nucleic acid molecules for polypeptide expression in plants using plant virus codon-bias 审中-公开
标题翻译：使用植物病毒密码子偏倚设计植物中多肽表达的核酸分子的方法和组合物
公开(公告)号：US20060236424A1
公开(公告)日：2006-10-19
申请号：US11399028
申请日：2006-04-05
申请人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Albert Lu , Billy McCutchen , Carl Simmons
发明人： Andre Abad , Ronald Flannagan , Rafael Herrmann , Albert Lu , Billy McCutchen , Carl Simmons
IPC分类号： A01H5/00 , A01H1/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.
摘要翻译：本发明涉及设计核酸分子以改进植物中编码多肽表达的方法。在这种方法中，密码子使用频率偏向于植物病毒，植物病毒组或其核酸分子子集的密码子使用频率。在优选的实施方案中，编码的多肽影响植物的表型。本发明还涉及编码杀虫多肽的核酸分子，其中核酸分子被设计为具有植物病毒密码子偏倚。本发明还涉及具有增加的杀虫多肽表达的转基因植物及其后代，以改善对对农业价值的植物有害的昆虫和其它害虫的抗性。

7. 发明申请

US20060288448A1 Insect-specific protease recognition sequences 有权
标题翻译：昆虫特异性蛋白酶识别序列
公开(公告)号：US20060288448A1
公开(公告)日：2006-12-21
申请号：US11448266
申请日：2006-06-07
申请人： Andre Abad , Gary Chun , Ronald Flannagan , Rafael Herrmann , Albert Lu , Janet Rice , Eric Schepers
发明人： Andre Abad , Gary Chun , Ronald Flannagan , Rafael Herrmann , Albert Lu , Janet Rice , Eric Schepers
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

8. 发明申请

US20070245427A1 Bacillus thuringiensis cry gene and protein 失效
标题翻译：苏云金芽孢杆菌cry基因和蛋白质
公开(公告)号：US20070245427A1
公开(公告)日：2007-10-18
申请号：US11404297
申请日：2006-04-14
申请人： Andre Abad , Ronald Flannagan , Billy McCutchen , Cao Yu
发明人： Andre Abad , Ronald Flannagan , Billy McCutchen , Cao Yu
IPC分类号： A01H5/00 , C07H21/04 , C12N15/82 , C07K14/415 , C12N5/04
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。特别地，提供了新型多核苷酸及其编码的杀虫多肽。还提供了使用本发明的新型多核苷酸和杀虫多肽保护植物免受害虫的方法。本发明的具体实施方案提供杀虫组合物和制剂，DNA构建体和转化的植物，植物细胞和种子。

9. 发明申请

US20070243536A1 Methods for identifying novel pesticidal gene homologues 有权
标题翻译：鉴定新型杀虫基因同源物的方法
公开(公告)号：US20070243536A1
公开(公告)日：2007-10-18
申请号：US11404741
申请日：2006-04-14
申请人： Andre Abad , Ronald Flannagan , Bin Hu , Billy McCutchen , Xiaofeng Yang , Cao Yu
发明人： Andre Abad , Ronald Flannagan , Bin Hu , Billy McCutchen , Xiaofeng Yang , Cao Yu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/689 , C12Q2541/101
摘要： Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly a Bacillus thuringiensis strain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.
摘要翻译：提供了鉴定新型杀虫基因同系物的方法和组合物。具体地说，本发明的方法包括系统地设计针对目的杀虫基因特异性的寡核苷酸引物，并对来自微生物特别是苏云金芽孢杆菌属菌株的核酸材料进行连续循环的PCR扩增，以鉴定已知杀虫基因的新型同系物。进一步公开了可用于实施本发明方法的寡核苷酸引物。

10. 发明申请

US20070245428A1 Bacillus thuringiensis cry gene and protein 有权
公开(公告)号：US20070245428A1
公开(公告)日：2007-10-18
申请号：US11404638
申请日：2006-04-14
申请人： Andre Abad , Ronald Flannagan , Billy McCutchen , Cao Yu
发明人： Andre Abad , Ronald Flannagan , Billy McCutchen , Cao Yu
IPC分类号： A01H5/00 , C07H21/04 , C12N15/82 , C07K14/415 , C12N5/04
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.

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