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1. 发明申请

US20050191613A1 Device for monitoring cells 有权
公开(公告)号：US20050191613A1
公开(公告)日：2005-09-01
申请号：US10957802
申请日：2004-10-04
申请人： J. Bruce Pitner , John Jacob Hemperly , Richard Guarino , Magdalena Wodnicka , David Stitt , Gregory Burrell , Timothy Foley , Patrick Shawn Beaty
发明人： J. Bruce Pitner , John Jacob Hemperly , Richard Guarino , Magdalena Wodnicka , David Stitt , Gregory Burrell , Timothy Foley , Patrick Shawn Beaty
IPC分类号： C12Q1/04 , C12Q1/18 , C12Q1/00
CPC分类号： C12Q1/18 , C12Q1/04 , C12Q2304/44 , G06F19/00 , Y02A90/26
摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.

2. 发明申请

US20070077651A1 Peptides for enhanced cell attachment and growth 有权
标题翻译：用于增强细胞附着和生长的肽
公开(公告)号：US20070077651A1
公开(公告)日：2007-04-05
申请号：US11604530
申请日：2006-11-27
申请人： Richard Guarino , Bryce Chaney , Andrea Liebmann-Vinson , Mohammad Heidaran
发明人： Richard Guarino , Bryce Chaney , Andrea Liebmann-Vinson , Mohammad Heidaran
IPC分类号： C12N5/08 , C12N5/06 , C12M3/00
CPC分类号： C07K7/06 , B33Y70/00 , B33Y80/00 , C07K5/0806 , C07K5/1008 , C07K5/1019
摘要： The present invention relates to cell adhesion promoting (“CAP”) peptide combinations that promote cell attachment or cell adhesion to culture surfaces that are otherwise cell adhesion resistant “CAR”. The invention provides combination of peptides that, when covalently coupled to a CAR layer such as hyaluronic acid that has been created on a polystyrene surface, promote cell attachment, growth differentiation, and execution of other desired cellular functions in culture.
摘要翻译：本发明涉及促进细胞附着或细胞粘附到培养物表面的细胞粘附促进（“CAP”）肽组合，否则细胞粘附抗性“CAR”。本发明提供了肽的组合，当共价偶联到CAR层（例如在聚苯乙烯表面上产生的透明质酸）共价偶联时，促进细胞附着，生长分化以及在培养中执行其它所需的细胞功能。

3. 发明申请

US20050143612A1 Production of alkyl aromatic compounds 有权
标题翻译：烷基芳族化合物的生产
公开(公告)号：US20050143612A1
公开(公告)日：2005-06-30
申请号：US10505787
申请日：2003-02-27
申请人： Shyh-Yuan Hwang , Maruti Bhandarkar , Chung-Ming Chi , Waheed Mukaddam , Frank Demers , Richard Guarino , Dana Johnson
发明人： Shyh-Yuan Hwang , Maruti Bhandarkar , Chung-Ming Chi , Waheed Mukaddam , Frank Demers , Richard Guarino , Dana Johnson
IPC分类号： C07C7/04 , C07B61/00 , C07C2/64 , C07C2/66 , C07C2/70 , C07C7/10 , C07C7/12 , C07C11/04 , C07C11/06 , C07C11/08 , C07C11/09 , C07C15/02 , C07C15/04 , C07C15/06 , C07C15/073 , C07C15/08 , C07C15/085
CPC分类号： C07C2/66 , C07C7/04 , C07C7/10 , C07C7/12 , C07C7/13 , C07C2529/08 , C07C2529/18 , C07C2529/40 , C07C2529/70 , C07C2529/82 , Y02P20/125 , C07C15/085 , C07C11/06 , C07C15/04
摘要： Improved integrated processes for the production of alkyl aromatic compounds are disclosed wherein aromatic compounds which may be treated for removal of deleterious substances are reacted with olefin compounds, which may also be treated for contaminant removal, in the presence of acidic zeolite catalyst(s) to produce the desired alkyl aromatic compound(s). The aromatic and preferably also the olefin feeds are treated substantially to remove contaminants, particularly the nitrogen compounds contained therein, before they are brought together for reaction in the presence of the zeolite catalyst(s). In accordance with the present invention, it has been found that feed pretreatment for removal of nitrogen compounds significantly improves the run length and life of the acidic zeolite catalyst(s). The feed pretreatment of this invention may include the steps of distillation, extraction, and/or adsorption by solid adsorbent, which may be regenerated in accordance with further embodiments of this invention.
摘要翻译：公开了用于生产烷基芳族化合物的改进的一体化方法，其中可以处理去除有害物质的芳族化合物与烯烃化合物反应，烯烃化合物也可以在酸性沸石催化剂存在下进行处理以除去污染物，产生所需的烷基芳族化合物。在沸石催化剂的存在下，芳族化合物和烯烃进料基本上被处理以除去污染物，特别是其中所含的氮化合物，然后将它们聚集在一起进行反应。根据本发明，已经发现用于除去氮化合物的进料预处理显着地改善了酸性沸石催化剂的运行长度和使用寿命。本发明的进料预处理可以包括根据本发明的其它实施方案可以再生的固体吸附剂的蒸馏，萃取和/或吸附的步骤。

4. 发明申请

US20050106727A1 Peptides for enhanced cell attachment and growth 有权
标题翻译：用于增强细胞附着和生长的肽
公开(公告)号：US20050106727A1
公开(公告)日：2005-05-19
申请号：US10670771
申请日：2003-09-26
申请人： Richard Guarino , Bryce Chaney , Andrea Liebmann-Vinson , Perry Haaland , Mohammad Heidaran
发明人： Richard Guarino , Bryce Chaney , Andrea Liebmann-Vinson , Perry Haaland , Mohammad Heidaran
IPC分类号： A61K20060101 , C07K5/083 , C07K5/103 , C07K5/11 , C07K7/06 , C07K14/78 , C07K17/02 , C12N5/02 , C12N5/06
CPC分类号： C07K7/06 , B33Y70/00 , B33Y80/00 , C07K5/0806 , C07K5/1008 , C07K5/1019
摘要： The present invention relates to cell adhesion promoting (“CAP”) peptide combinations that promote cell attachment or cell adhesion to culture surfaces that are otherwise cell adhesion resistant “CAR”. The invention provides combination of peptides that, when covalently coupled to a CAR layer such as hyaluronic acid that has been created on a polystyrene surface, promote cell attachment, growth differentiation, and execution of other desired cellular functions in culture.
摘要翻译：本发明涉及促进细胞附着或细胞粘附到培养物表面的细胞粘附促进（“CAP”）肽组合，否则细胞粘附抗性“CAR”。本发明提供了肽的组合，当共价偶联到CAR层（例如在聚苯乙烯表面上产生的透明质酸）共价偶联时，促进细胞附着，生长分化以及在培养中执行其它所需的细胞功能。

5. 发明申请

US20050058687A1 Covalently attached collagen VI for cell attachment and proliferation 审中-公开
标题翻译：共价连接胶原蛋白VI用于细胞附着和增殖
公开(公告)号：US20050058687A1
公开(公告)日：2005-03-17
申请号：US10660781
申请日：2003-09-12
申请人： Richard Guarino , Jonathan Rowley , Andrea Liebmann-Vinson , John Hemperly , Mohammad Heidaran , Sharon Presnell
发明人： Richard Guarino , Jonathan Rowley , Andrea Liebmann-Vinson , John Hemperly , Mohammad Heidaran , Sharon Presnell
IPC分类号： C12N5/00 , C12N5/02 , C12N5/08 , G01N33/543
CPC分类号： G01N33/54393 , C12N5/0068 , C12N2500/90 , C12N2533/52 , C12N2533/54 , C12N2533/80
摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.
摘要翻译：用于细胞培养物的表面包括与CAR材料结合的载体，并结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体）），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。还公开了包含表面和附加试剂的试剂盒。

6. 发明申请

US20100273844A1 PHARMACEUTICAL COMPOSITIONS FOR TREATING OR PREVENTING MIGAINES 审中-公开
标题翻译：用于治疗或预防移植的药物组合物
公开(公告)号：US20100273844A1
公开(公告)日：2010-10-28
申请号：US11992714
申请日：2006-09-27
申请人： Richard Guarino , Eyal S. Ron
发明人： Richard Guarino , Eyal S. Ron
IPC分类号： A61K31/42 , C07D261/18 , A61P25/06
CPC分类号： A61K31/137
摘要： The present invention provides a method for treating or preventing one or more types of migraines by administering a monoamine oxidase inhibitor, preferably isocarboxazid, in a rapid-release formulation. The present invention also provides a pharmaceutical composition suitable for treating or preventing migraines and having at least one monoamine oxidase inhibitor.
摘要翻译：本发明提供了通过在快速释放制剂中施用单胺氧化酶抑制剂（优选异卡波肼）来治疗或预防一种或多种类型的偏头痛的方法。本发明还提供了适用于治疗或预防偏头痛并具有至少一种单胺氧化酶抑制剂的药物组合物。

7. 发明申请

US20050059150A1 Environments that maintain function of primary liver cells 审中-公开
标题翻译：维持原发性肝细胞功能的环境
公开(公告)号：US20050059150A1
公开(公告)日：2005-03-17
申请号：US10664037
申请日：2003-09-17
申请人： Richard Guarino , Sharon Presnell , Andrea Liebmann-Vinson , Mohammad Heidaran
发明人： Richard Guarino , Sharon Presnell , Andrea Liebmann-Vinson , Mohammad Heidaran
IPC分类号： C12N5/071 , G01N33/50 , C12N5/08
CPC分类号： G01N33/5067 , C12N5/067 , C12N5/0671 , C12N2533/32 , C12N2533/54
摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.
摘要翻译：用于细胞培养的表面包括与CAR材料结合的载体，并结合CAR材料，ECM蛋白或其生物活性片段或变体如弹性蛋白，纤连蛋白，玻连蛋白，层粘连蛋白，胶原I，胶原 III，胶原IV和胶原蛋白VI。此外，表面上任选存在的是活性因子，优选聚阳离子聚合物或其生物活性片段或变体，例如聚乙烯亚胺（PEI），聚-D-赖氨酸（PDL），聚-L-赖氨酸（PLL），聚-D-鸟氨酸（PDO）或聚-L-鸟氨酸（PLO）。该表面用于细胞培养以促进原代肝细胞的细胞附着，存活和/或增殖。本发明还涉及利用该表面的方法，例如细胞附着，存活和/或增殖的方法。还公开了使用无血清培养基的细胞培养物中的表面。还公开了使用本发明的表面进行筛选的方法。

8. 发明申请

US20070092861A1 Environments that maintain function of primary liver cells 审中-公开
标题翻译：维持原发性肝细胞功能的环境
公开(公告)号：US20070092861A1
公开(公告)日：2007-04-26
申请号：US10570702
申请日：2004-09-10
申请人： Richard Guarino , Sharon Presnell , Andrea Liebmann-Vinson , Mohammed Heidaran , John Hemperly , Stewart Jurgensen , Ronald Laethem , Neil Robbins
发明人： Richard Guarino , Sharon Presnell , Andrea Liebmann-Vinson , Mohammed Heidaran , John Hemperly , Stewart Jurgensen , Ronald Laethem , Neil Robbins
IPC分类号： C12Q1/00 , C12N5/08
CPC分类号： G01N33/5067 , C12N5/067 , C12N5/0671 , C12N2533/32 , C12N2533/54
摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, lamninin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, maintenance of function, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, maintenance of function, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.
摘要翻译：用于细胞培养的表面包括与CAR材料结合的载体，并结合CAR材料，ECM蛋白或其生物活性片段或变体如弹性蛋白，纤连蛋白，玻连蛋白，lamninin，胶原I，胶原 III，胶原IV和胶原蛋白VI。此外，表面上任选存在的是活性因子，优选聚阳离子聚合物或其生物活性片段或变体，例如聚乙烯亚胺（PEI），聚-D-赖氨酸（PDL），聚-L-赖氨酸（PLL），聚-D-鸟氨酸（PDO）或聚-L-鸟氨酸（PLO）。该表面用于细胞培养以促进细胞附着，存活，功能的维持和/或原代肝细胞的增殖。本发明还涉及利用该表面的方法，例如附着，存活，维持功能和/或细胞增殖的方法。还公开了使用无血清培养基的细胞培养物中的表面。还公开了使用本发明的表面进行筛选的方法。

9. 发明申请

US20060105455A1 Covalently attached collagen VI for cell attachment and proliferation 审中-公开
标题翻译：共价连接胶原蛋白VI用于细胞附着和增殖
公开(公告)号：US20060105455A1
公开(公告)日：2006-05-18
申请号：US11322637
申请日：2005-12-30
申请人： Richard Guarino , Jonathan Rowley , Andrea Liebmann-Vinson , John Hemperly , Mohammad Heidaran , Sharon Presnell
发明人： Richard Guarino , Jonathan Rowley , Andrea Liebmann-Vinson , John Hemperly , Mohammad Heidaran , Sharon Presnell
IPC分类号： C12N5/08
CPC分类号： G01N33/54393 , C12N5/0068 , C12N2500/90 , C12N2533/52 , C12N2533/54 , C12N2533/80
摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.
摘要翻译：用于细胞培养物的表面包括与CAR材料结合的载体，并且结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体）），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。还公开了包含表面和附加试剂的试剂盒。

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