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1. 发明申请

US20050058687A1 Covalently attached collagen VI for cell attachment and proliferation 审中-公开
标题翻译：共价连接胶原蛋白VI用于细胞附着和增殖
公开(公告)号：US20050058687A1
公开(公告)日：2005-03-17
申请号：US10660781
申请日：2003-09-12
申请人： Richard Guarino , Jonathan Rowley , Andrea Liebmann-Vinson , John Hemperly , Mohammad Heidaran , Sharon Presnell
发明人： Richard Guarino , Jonathan Rowley , Andrea Liebmann-Vinson , John Hemperly , Mohammad Heidaran , Sharon Presnell
IPC分类号： C12N5/00 , C12N5/02 , C12N5/08 , G01N33/543
CPC分类号： G01N33/54393 , C12N5/0068 , C12N2500/90 , C12N2533/52 , C12N2533/54 , C12N2533/80
摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.
摘要翻译：用于细胞培养物的表面包括与CAR材料结合的载体，并结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体）），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。还公开了包含表面和附加试剂的试剂盒。

2. 发明申请

US20060105455A1 Covalently attached collagen VI for cell attachment and proliferation 审中-公开
标题翻译：共价连接胶原蛋白VI用于细胞附着和增殖
公开(公告)号：US20060105455A1
公开(公告)日：2006-05-18
申请号：US11322637
申请日：2005-12-30
申请人： Richard Guarino , Jonathan Rowley , Andrea Liebmann-Vinson , John Hemperly , Mohammad Heidaran , Sharon Presnell
发明人： Richard Guarino , Jonathan Rowley , Andrea Liebmann-Vinson , John Hemperly , Mohammad Heidaran , Sharon Presnell
IPC分类号： C12N5/08
CPC分类号： G01N33/54393 , C12N5/0068 , C12N2500/90 , C12N2533/52 , C12N2533/54 , C12N2533/80
摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.
摘要翻译：用于细胞培养物的表面包括与CAR材料结合的载体，并且结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体）），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。还公开了包含表面和附加试剂的试剂盒。

3. 发明申请

US20050059150A1 Environments that maintain function of primary liver cells 审中-公开
标题翻译：维持原发性肝细胞功能的环境
公开(公告)号：US20050059150A1
公开(公告)日：2005-03-17
申请号：US10664037
申请日：2003-09-17
申请人： Richard Guarino , Sharon Presnell , Andrea Liebmann-Vinson , Mohammad Heidaran
发明人： Richard Guarino , Sharon Presnell , Andrea Liebmann-Vinson , Mohammad Heidaran
IPC分类号： C12N5/071 , G01N33/50 , C12N5/08
CPC分类号： G01N33/5067 , C12N5/067 , C12N5/0671 , C12N2533/32 , C12N2533/54
摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, laminin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium. Methods of screening using the surface of the invention are also disclosed.
摘要翻译：用于细胞培养的表面包括与CAR材料结合的载体，并结合CAR材料，ECM蛋白或其生物活性片段或变体如弹性蛋白，纤连蛋白，玻连蛋白，层粘连蛋白，胶原I，胶原 III，胶原IV和胶原蛋白VI。此外，表面上任选存在的是活性因子，优选聚阳离子聚合物或其生物活性片段或变体，例如聚乙烯亚胺（PEI），聚-D-赖氨酸（PDL），聚-L-赖氨酸（PLL），聚-D-鸟氨酸（PDO）或聚-L-鸟氨酸（PLO）。该表面用于细胞培养以促进原代肝细胞的细胞附着，存活和/或增殖。本发明还涉及利用该表面的方法，例如细胞附着，存活和/或增殖的方法。还公开了使用无血清培养基的细胞培养物中的表面。还公开了使用本发明的表面进行筛选的方法。

4. 发明申请

US20050106727A1 Peptides for enhanced cell attachment and growth 有权
标题翻译：用于增强细胞附着和生长的肽
公开(公告)号：US20050106727A1
公开(公告)日：2005-05-19
申请号：US10670771
申请日：2003-09-26
申请人： Richard Guarino , Bryce Chaney , Andrea Liebmann-Vinson , Perry Haaland , Mohammad Heidaran
发明人： Richard Guarino , Bryce Chaney , Andrea Liebmann-Vinson , Perry Haaland , Mohammad Heidaran
IPC分类号： A61K20060101 , C07K5/083 , C07K5/103 , C07K5/11 , C07K7/06 , C07K14/78 , C07K17/02 , C12N5/02 , C12N5/06
CPC分类号： C07K7/06 , B33Y70/00 , B33Y80/00 , C07K5/0806 , C07K5/1008 , C07K5/1019
摘要： The present invention relates to cell adhesion promoting (“CAP”) peptide combinations that promote cell attachment or cell adhesion to culture surfaces that are otherwise cell adhesion resistant “CAR”. The invention provides combination of peptides that, when covalently coupled to a CAR layer such as hyaluronic acid that has been created on a polystyrene surface, promote cell attachment, growth differentiation, and execution of other desired cellular functions in culture.
摘要翻译：本发明涉及促进细胞附着或细胞粘附到培养物表面的细胞粘附促进（“CAP”）肽组合，否则细胞粘附抗性“CAR”。本发明提供了肽的组合，当共价偶联到CAR层（例如在聚苯乙烯表面上产生的透明质酸）共价偶联时，促进细胞附着，生长分化以及在培养中执行其它所需的细胞功能。

5. 发明申请

US20070077651A1 Peptides for enhanced cell attachment and growth 有权
标题翻译：用于增强细胞附着和生长的肽
公开(公告)号：US20070077651A1
公开(公告)日：2007-04-05
申请号：US11604530
申请日：2006-11-27
申请人： Richard Guarino , Bryce Chaney , Andrea Liebmann-Vinson , Mohammad Heidaran
发明人： Richard Guarino , Bryce Chaney , Andrea Liebmann-Vinson , Mohammad Heidaran
IPC分类号： C12N5/08 , C12N5/06 , C12M3/00
CPC分类号： C07K7/06 , B33Y70/00 , B33Y80/00 , C07K5/0806 , C07K5/1008 , C07K5/1019
摘要： The present invention relates to cell adhesion promoting (“CAP”) peptide combinations that promote cell attachment or cell adhesion to culture surfaces that are otherwise cell adhesion resistant “CAR”. The invention provides combination of peptides that, when covalently coupled to a CAR layer such as hyaluronic acid that has been created on a polystyrene surface, promote cell attachment, growth differentiation, and execution of other desired cellular functions in culture.
摘要翻译：本发明涉及促进细胞附着或细胞粘附到培养物表面的细胞粘附促进（“CAP”）肽组合，否则细胞粘附抗性“CAR”。本发明提供了肽的组合，当共价偶联到CAR层（例如在聚苯乙烯表面上产生的透明质酸）共价偶联时，促进细胞附着，生长分化以及在培养中执行其它所需的细胞功能。

6. 发明申请

US20050036980A1 Peptides for enhanced cell attachment and cell growth 有权
标题翻译：用于增强细胞附着和细胞生长的肽
公开(公告)号：US20050036980A1
公开(公告)日：2005-02-17
申请号：US10641286
申请日：2003-08-15
申请人： Bryce Chaney , Mohammad Heidaran , Andrea Liebmann-Vinson
发明人： Bryce Chaney , Mohammad Heidaran , Andrea Liebmann-Vinson
IPC分类号： C07K5/083 , C07K7/06 , C07K17/06 , C08J7/12 , C12N5/077 , A61K38/08 , C08G63/48 , C08G63/91
CPC分类号： A61K38/08 , C07K5/0806 , C07K7/06 , C07K17/06 , C08J7/12 , C12N5/0654 , C12N5/0656 , C12N2533/30 , C12N2533/50
摘要： The present invention relates to cell adhesion promoting (“CAP”) peptide combinations that promote cell attachment or cell adhesion to culture surfaces that are otherwise cell adhesion resistant “CAR”. The invention provides combination of peptides that, when covalently coupled to a CAR layer such as hyaluronic acid that has been created on a polystyrene surface, promote cell attachment, growth differentiation, and execution of other desired cellular functions in culture.
摘要翻译：本发明涉及促进细胞附着或细胞粘附到培养物表面的细胞粘附促进（“CAP”）肽组合，否则细胞粘附抗性“CAR”。本发明提供了肽的组合，当共价偶联到CAR层（例如在聚苯乙烯表面上产生的透明质酸）共价偶联时，促进细胞附着，生长分化以及在培养中执行其它所需的细胞功能。

7. 发明申请

US20050059083A1 High throughput method to identify ligands for cell attachment 审中-公开
标题翻译：用于鉴定细胞附着配体的高通量方法
公开(公告)号：US20050059083A1
公开(公告)日：2005-03-17
申请号：US10662640
申请日：2003-09-15
申请人： Andrea Liebmann-Vinson , Jonathan Rowley , Chris Bodily , Mohammad Heidaran
发明人： Andrea Liebmann-Vinson , Jonathan Rowley , Chris Bodily , Mohammad Heidaran
IPC分类号： G01N33/50 , G01N33/543 , G01N33/53
CPC分类号： G01N33/5011 , G01N33/5008 , G01N33/502 , G01N33/5029 , G01N33/54393
摘要： A high throughput method is provided for identifying agents capable of producing a desired biological response in whole cells. The method includes the steps of providing receptacles having a culture surface; placing different mixtures of single agents into selective ones of the receptacles according to a statistical design; and immobilizing the mixtures of single agents to the culture surface. The method further includes contacting the immobilized agents with the whole cells; and acquiring data which is indicative of a desired biological response in the contacted cells. The method also includes using statistical modeling of the acquired data to determine which mixtures of single agents and/or which single agents in these mixtures are effective in producing the desired biological response in the contacted cells.
摘要翻译：提供了一种高通量方法，用于鉴定能够在全细胞中产生所需生物反应的试剂。该方法包括提供具有培养表面的容器的步骤; 根据统计设计将单一试剂的不同混合物置于选择性容器中; 并将单一试剂的混合物固定到培养表面。该方法还包括使固定化的试剂与整个细胞接触; 并且获取指示所接触细胞中所需生物反应的数据。该方法还包括使用获取的数据的统计建模来确定单个试剂的哪些混合物和/或这些混合物中的哪一种试剂在接触的细胞中产生所需的生物反应是有效的。

8. 发明授权

US07790458B2 Material and methods for the growth of hematopoietic stem cells 有权
标题翻译：造血干细胞生长的材料和方法
公开(公告)号：US07790458B2
公开(公告)日：2010-09-07
申请号：US11128950
申请日：2005-05-13
申请人： Ruiling Xu , Andrea Liebmann-Vinson , Keith DeLuca , Mohammad Heidaran
发明人： Ruiling Xu , Andrea Liebmann-Vinson , Keith DeLuca , Mohammad Heidaran
IPC分类号： C12N5/02 , C12N5/00 , C12N5/071
CPC分类号： C12N5/0647 , C12N5/0663 , C12N2500/25 , C12N2500/36 , C12N2500/42 , C12N2500/90 , C12N2501/10 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/125 , C12N2501/13 , C12N2501/135 , C12N2501/14 , C12N2501/145 , C12N2501/15 , C12N2501/155 , C12N2501/165 , C12N2501/21 , C12N2501/22 , C12N2501/23 , C12N2501/2303 , C12N2501/2305 , C12N2501/2306 , C12N2501/2311 , C12N2501/235 , C12N2501/26 , C12N2501/32 , C12N2501/39 , C12N2501/415 , C12N2501/58 , C12N2501/60 , C12N2501/70 , C12N2501/999 , C12N2533/52 , C12N2533/54 , C12N2533/70
摘要： The invention provides populations of expanded CD34-expressing cells and methods of use. Particular embodiments provide for defined culture media useful for growing these cells, and grafts comprising these cells. The invention finds use in methods for reconstituting, repairing, and regenerating tissue damage.
摘要翻译：本发明提供了扩增的CD34表达细胞和使用方法的群体。具体实施方案提供用于生长这些细胞的限定的培养基和包含这些细胞的移植物。本发明用于重组，修复和再生组织损伤的方法。

9. 发明申请

US20050153429A1 Cell adhesion resisting surfaces 审中-公开
标题翻译：细胞粘附抗性表面
公开(公告)号：US20050153429A1
公开(公告)日：2005-07-14
申请号：US10994879
申请日：2004-11-22
申请人： Andrea Liebmann-Vinson , Richard Clarke , Ruiling Xu , Mohammad Heidaran
发明人： Andrea Liebmann-Vinson , Richard Clarke , Ruiling Xu , Mohammad Heidaran
IPC分类号： C12N5/00 , G01N33/53 , B05D3/00 , C12M1/34
CPC分类号： C12N5/0068 , C12N2533/80 , Y10T428/13 , Y10T428/1352
摘要： A coated surface that resists cell adhesion comprises hyaluronic acid directly bound to a plasma-treated polymer surface. A process for producing the coated surface is disclosed as are further modifications of the hyaluronic acid by attaching ligand-binding polypeptides (antibodies or antibody binding proteins).
摘要翻译：抗细胞粘附的涂覆表面包括与等离子体处理的聚合物表面直接结合的透明质酸。通过连接配体结合多肽（抗体或抗体结合蛋白），透明质酸的进一步修饰也被公开。

10. 发明申请

US20060122104A1 Methods for in vitro expansion and transdifferentiation of human pancreatic acinar cells into insulin-producing cells 审中-公开
标题翻译：人胰腺细胞体外扩增和转分化为胰岛素生成细胞的方法
公开(公告)号：US20060122104A1
公开(公告)日：2006-06-08
申请号：US10515421
申请日：2003-05-22
申请人： Sharon Presnell , Mohammad Heidaran , Perry Haaland , Neil Robbins , David Scharp , Paul Latta , Margaret Coutts , Catherine McIntyre
发明人： Sharon Presnell , Mohammad Heidaran , Perry Haaland , Neil Robbins , David Scharp , Paul Latta , Margaret Coutts , Catherine McIntyre
IPC分类号： C12N5/08 , A61K38/18
CPC分类号： C12N5/0676 , A61K35/12 , A61K2035/126 , C12N5/0037 , C12N2500/25 , C12N2500/38 , C12N2500/46 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/113 , C12N2501/115 , C12N2501/117 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/16 , C12N2501/165 , C12N2501/235 , C12N2501/315 , C12N2501/335 , C12N2501/34 , C12N2501/345 , C12N2501/35 , C12N2501/37 , C12N2501/39 , C12N2501/392 , C12N2501/41 , C12N2501/83 , C12N2501/85 , C12N2501/998 , C12N2506/22 , Y02A50/471
摘要： This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype (IP cells) showing characteristics of acinar cells and liver cells. The invention also relates to a method for transforming these IP cells to insulin-producing cells in vitro, comprising culturing the cells in a novel, defined medium. Also disclosed are suitable culture media for performing these methods, isolated cells having the phenotype of IP cells and/or produced by these methods, and kits for performing the methods.
摘要翻译：本发明涉及例如扩展哺乳动物腺泡细胞的方法，包括在包括细胞培养基和细胞附着表面的细胞培养系统中培养细胞，其中腺泡细胞与转化为显示腺泡细胞和肝细胞特征的修饰的细胞表型（IP细胞）。本发明还涉及在体外将这些IP细胞转化成胰岛素生成细胞的方法，包括在新颖的限定培养基中培养细胞。还公开了用于进行这些方法的合适的培养基，具有IP细胞表型和/或通过这些方法产生的分离的细胞，以及用于进行该方法的试剂盒。

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