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1. 发明授权

US07482119B2 Solid phase methods for polynucleotide production 有权
标题翻译：用于多核苷酸生产的固相方法
公开(公告)号：US07482119B2
公开(公告)日：2009-01-27
申请号：US10965018
申请日：2004-10-13
申请人： Hsing-Yeh Parker , John T. Mulligan
发明人： Hsing-Yeh Parker , John T. Mulligan
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12P19/34 , B01J19/0046 , B01J2219/00286 , B01J2219/00308 , B01J2219/00351 , B01J2219/00353 , B01J2219/00389 , B01J2219/00495 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00689 , B01J2219/00698 , B01J2219/00722 , C07H21/00
摘要： Polynucleotides having in excess of 1,000 nucleotides can be prepared using a solid phase synthesis technique. A feature of the technique is the use of a reusable solid support that contains covalently bound oligonucleotide. This covalently bound oligonucleotide is annealed to a bridge oligonucleotide, where the bridge is also annealed to a first oligonucleotide that forms a portion of the target polynucleotide. After the target polynucleotide is synthesized, it can be removed from the solid support under denaturing conditions, and the solid support re-used to prepare additional target polynucleotides. The yield of the target polynucleotide increases when shearing force is applied to the solid support that is linked to the growing oligonucleotide. This shearing force is thought to extend the growing end of the oligonucleotide away from contact with other oligonucleotide bound to the solid support and make that end more accessible to annealing with solution oligonucleotide. The synthesis is conveniently accomplished on a porous frit, where reagents and washing solutions are pumped through the frit.
摘要翻译：具有超过1,000个核苷酸的多核苷酸可以使用固相合成技术制备。该技术的特征是使用含有共价结合的寡核苷酸的可重复使用的固体支持物。将共价结合的寡核苷酸与桥寡核苷酸退火，其中桥还与形成靶多核苷酸的一部分的第一寡核苷酸退火。在合成靶多核苷酸之后，可以在变性条件下将其从固体支持物上除去，并且固体支持物再次用于制备另外的靶多核苷酸。当剪切力施加到与生长的寡核苷酸连接的固体支持物上时，靶多核苷酸的产量增加。认为这种剪切力使得寡核苷酸的生长末端远离与与固体支持物结合的其它寡核苷酸的接触，并使得该结果更容易用溶液寡核苷酸退火。合成方便地在多孔玻璃料上完成，其中试剂和洗涤溶液通过玻璃料泵送。

2. 发明申请

US20090137027A1 SOLID PHASE METHODS FOR POLYNUCLEOTIDE PRODUCTION 审中-公开
标题翻译：用于多核苷酸生产的固相方法
公开(公告)号：US20090137027A1
公开(公告)日：2009-05-28
申请号：US12336412
申请日：2008-12-16
申请人： Hsing-Yeh Parker , John T. Mulligan
发明人： Hsing-Yeh Parker , John T. Mulligan
IPC分类号： C12M1/36 , C12M1/00
CPC分类号： C12P19/34 , B01J19/0046 , B01J2219/00286 , B01J2219/00308 , B01J2219/00351 , B01J2219/00353 , B01J2219/00389 , B01J2219/00495 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00689 , B01J2219/00698 , B01J2219/00722 , C07H21/00
摘要： Polynucleotides having in excess of 1,000 nucleotides can be prepared using a solid phase synthesis technique. A feature of the technique is the use of a reusable solid support that contains covalently bound oligonucleotide. This covalently bound oligonucleotide is annealed to a bridge oligonucleotide, where the bridge is also annealed to a first oligonucleotide that forms a portion of the target polynucleotide. After the target polynucleotide is synthesized, it can be removed from the solid support under denaturing conditions, and the solid support re-used to prepare additional target polynucleotides. The yield of the target polynucleotide increases when shearing force is applied to the solid support that is linked to the growing oligonucleotide. This shearing force is thought to extend the growing end of the oligonucleotide away from contact with other oligonucleotide bound to the solid support and make that end more accessible to annealing with solution oligonucleotide. The synthesis is conveniently accomplished on a porous frit, where reagents and washing solutions are pumped through the frit.
摘要翻译：具有超过1,000个核苷酸的多核苷酸可以使用固相合成技术制备。该技术的特征是使用含有共价结合的寡核苷酸的可重复使用的固体支持物。将共价结合的寡核苷酸与桥寡核苷酸退火，其中桥还与形成靶多核苷酸的一部分的第一寡核苷酸退火。在合成靶多核苷酸之后，可以在变性条件下将其从固体支持物上除去，并且固体支持物再次用于制备另外的靶多核苷酸。当剪切力施加到与生长的寡核苷酸连接的固体支持物上时，靶多核苷酸的产量增加。认为这种剪切力使得寡核苷酸的生长末端远离与与固体支持物结合的其它寡核苷酸的接触，并使得该结果更容易用溶液寡核苷酸退火。合成方便地在多孔玻璃料上完成，其中试剂和洗涤溶液通过玻璃料泵送。

3. 发明授权

US07994299B2 Compositions and methods for increasing bone mineralization 有权
公开(公告)号：US07994299B2
公开(公告)日：2011-08-09
申请号：US11931768
申请日：2007-10-31
申请人： Mary E. Brunkow , David J. Galas , Brian Kovacevich , John T. Mulligan , Bryan W. Paeper , Jeffrey Van Ness , David J. Winkler
发明人： Mary E. Brunkow , David J. Galas , Brian Kovacevich , John T. Mulligan , Bryan W. Paeper , Jeffrey Van Ness , David J. Winkler
IPC分类号： C07H21/04 , C12N15/00
CPC分类号： G01N33/74 , A01K2217/05 , A01K2217/075 , A61K38/18 , A61K39/3955 , A61K45/06 , A61K48/00 , C07K14/475 , C07K14/51 , C07K16/18 , C07K16/22 , C07K2317/11 , C07K2317/23 , C07K2319/00 , C12N2799/021 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/172 , G01N33/6872 , G01N33/84 , G01N2333/47 , G01N2333/495 , G01N2333/51 , G01N2500/02 , G01N2500/20
摘要： A novel class or family of TGF-β binding proteins is disclosed. Also disclosed are assays for selecting molecules for increasing bone mineralization and methods for utilizing such molecules.

4. 发明授权

US06664112B2 Methods for improving the sequence fidelity of synthetic double-stranded oligonucleotides 有权
公开(公告)号：US06664112B2
公开(公告)日：2003-12-16
申请号：US09872761
申请日：2001-06-01
申请人： John T. Mulligan , John C. Tabone
发明人： John T. Mulligan , John C. Tabone
IPC分类号： G01N3300
CPC分类号： C07H21/00 , C07H1/06 , C12N15/101 , Y10T436/143333
摘要： Synthetic oligonucleotides, such as synthetic DNA, often contain sequence errors due to synthetic failures (e.g., side products and/or truncated products). Methods are provided herein for improving the sequence fidelity of synthetic double-stranded oligonucleotides by separative depletion of synthetic failures. Separation is effected by utilization of methodologies in a preparative mode under denaturing conditions. A preferred use of the methods relates to gene synthesis.

5. 发明授权

US06623928B2 Methods and compositions for determining the sequence of nucleic acid molecules 有权
公开(公告)号：US06623928B2
公开(公告)日：2003-09-23
申请号：US09855999
申请日：2001-05-14
申请人： Jeffrey Van Ness , John C. Tabone , J. Jeffry Howbert , John T. Mulligan
发明人： Jeffrey Van Ness , John C. Tabone , J. Jeffry Howbert , John T. Mulligan
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07B2200/11 , C07D211/78 , C07H21/04 , C12Q1/6872 , C12Q1/6874 , C40B40/00 , Y10T436/24 , C12Q2565/629 , C12Q2563/167
摘要： Methods and compounds, including compositions therefrom, are provided for determining the sequence of nucleic acid molecules. The methods permit the determination of multiple nucleic acid sequences simultaneously. The compounds are used as tags to generate tagged nucleic acid fragments which are complementary to a selected target nucleic acid molecule. Each tag is correlative with a particular nucleotide and, in a preferred embodiment, is detectable by mass spectrometry. Following separation of the tagged fragments by sequential length, the tags are cleaved from the tagged fragments. In a preferred embodiment, the tags are detected by mass spectrometry and the sequence of the nucleic acid molecule is determined therefrom. The individual steps of the methods can be used in automated format, e.g., by the incorporation into systems.

6. 发明授权

US06613508B1 Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques 失效
标题翻译：利用施胶技术分析核酸分子的方法和组合物
公开(公告)号：US06613508B1
公开(公告)日：2003-09-02
申请号：US08898564
申请日：1997-07-22
申请人： Jeffrey Van Ness , John C. Tabone , J. Jeffry Howbert , John T. Mulligan
发明人： Jeffrey Van Ness , John C. Tabone , J. Jeffry Howbert , John T. Mulligan
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C07B2200/11 , C12Q1/6825 , C12Q1/6846 , C12Q1/6872 , C40B40/00 , C12Q2563/149 , C12Q2563/113 , C12Q2563/167 , C12Q2525/191
摘要： Tags and linkers specifically designed for a wide variety of nucleic acid reactions are disclosed, which are suitable for a wide variety of nucleic acid reactions wherein separation of nucleic acid molecules based upon size is required.
摘要翻译：公开了针对各种核酸反应专门设计的标签和接头，其适用于各种各样的核酸反应，其中需要基于尺寸分离核酸分子。

7. 发明授权

US08089871B2 Method and apparatus for traffic control of dynamic denial of service attacks within a communications network 有权
标题翻译：用于通信网络内动态拒绝服务攻击的流量控制的方法和装置
公开(公告)号：US08089871B2
公开(公告)日：2012-01-03
申请号：US11090634
申请日：2005-03-25
申请人： Ali M. Iloglu , Han Q. Nguyen , John T. Mulligan , Samir S. Saad
发明人： Ali M. Iloglu , Han Q. Nguyen , John T. Mulligan , Samir S. Saad
IPC分类号： G01R31/08 , G06F7/04 , G06F11/00 , G06F12/14 , H04L12/28 , H04L12/56
CPC分类号： H04L63/1408 , H04L63/1458 , H04L2463/141
摘要： A method and apparatus for providing traffic management for distributed denial of service (DDOS) traffic. Within a communications network, a DDOS detection system monitors network traffic to identify traffic that is designed to attack a particular server within the network and their entry points into the network. A traffic routing control unit is requested to deny service to the DDOS traffic. By selectively manipulating the routing information propagated to network edge routers, the traffic that is denied service is limited to mostly DDOS traffic and is routed to a cleaning center or a null address in the most effective fashion.
摘要翻译：一种用于为分散式拒绝服务（DDOS）流量提供流量管理的方法和装置。在通信网络中，DDOS检测系统监视网络流量以识别旨在攻击网络内的特定服务器及其进入网络的流量。请求流量路由控制单元拒绝对DDOS流量的服务。通过选择性地操纵传播到网络边缘路由器的路由信息，被拒绝服务的流量主要被限制在DDOS流量中，并以最有效的方式被路由到清洁中心或空地址。

8. 发明授权

US6090620A Genes and gene products related to Werner's syndrome 失效
标题翻译：与维纳综合征相关的基因和基因产物
公开(公告)号：US6090620A
公开(公告)日：2000-07-18
申请号：US781891
申请日：1996-12-27
申请人： Ying-Hui Fu , Chang-En Yu , Junko Oshima , John T. Mulligan , Gerard D. Schellenberg
发明人： Ying-Hui Fu , Chang-En Yu , Junko Oshima , John T. Mulligan , Gerard D. Schellenberg
IPC分类号： A01K67/027 , A01K67/00 , C07H21/04 , C07K14/00 , C07K14/47 , C07K16/18 , C12N5/10 , C12N9/64 , C12N9/90 , C12N15/09 , C12N15/861 , C12N15/867 , C12P21/08 , C12Q1/68 , C12N5/00 , C12N15/00 , C12N15/63
CPC分类号： C07K14/47 , C12N9/90 , A01K2217/05 , C12N2799/021
摘要： The present invention discloses nucleic acid molecules encoding WRN gene products, expresion vectors, viral vectors, and host cells suitable for expressing such products.
摘要翻译：本发明公开了编码WRN基因产物，表达载体，病毒载体和适于表达该产物的宿主细胞的核酸分子。

9. 发明申请

US20120147894A1 METHODS AND APPARATUS TO PROVISION CLOUD COMPUTING NETWORK ELEMENTS 有权
标题翻译：提供云计算网络元素的方法和设备
公开(公告)号：US20120147894A1
公开(公告)日：2012-06-14
申请号：US12963363
申请日：2010-12-08
申请人： John T. Mulligan , David John Knight , Ronnie R. Kulper , Don J. Moloney , Mai-Uyen Thi Nguyen , Han Q. Nguyen , Diana Lynn Toll-Verry , Simon S. Zelingher
发明人： John T. Mulligan , David John Knight , Ronnie R. Kulper , Don J. Moloney , Mai-Uyen Thi Nguyen , Han Q. Nguyen , Diana Lynn Toll-Verry , Simon S. Zelingher
IPC分类号： H04L12/28 , G06F9/455
CPC分类号： H04L41/0803 , G06F9/45533 , H04L41/5048 , H04L41/5096 , H04L45/02 , H04L47/70 , H04L49/70
摘要： Methods and apparatus to provision cloud computing network elements are disclosed. A disclosed example method includes receiving a selection of a cloud networking template from a client, wherein the cloud networking template includes a data center connector type and a wide area network connector type, configuring a virtual machine on a host server based on the cloud networking template, configuring a data center connector based on the data center connector type, configuring a wide area network connector based on the wide area network connector type, and coupling the wide area network connector to the data center connector and coupling the data center connector to the virtual machine within the host server to enable the client to access the virtual machine.
摘要翻译：公开了提供云计算网元的方法和装置。所公开的示例方法包括从客户端接收云组网模板的选择，其中所述云组网模板包括数据中心连接器类型和广域网连接器类型，基于所述云网络模板在主机服务器上配置虚拟机，基于数据中心连接器类型配置数据中心连接器，基于广域网连接器类型配置广域网连接器，并将广域网连接器连接到数据中心连接器，并将数据中心连接器耦合到虚拟主机服务器中的机器，以使客户端能够访问虚拟机。

10. 发明授权

US06803453B1 Antibodies associated with alterations in bone density 有权
标题翻译：与骨密度变化相关的抗体
公开(公告)号：US06803453B1
公开(公告)日：2004-10-12
申请号：US09668021
申请日：2000-09-21
申请人： Mary E. Brunkow , David J. Galas , Brian Kovacevich , John T. Mulligan , Bryan W. Paeper , Jeffrey Van Ness , David G. Winkler
发明人： Mary E. Brunkow , David J. Galas , Brian Kovacevich , John T. Mulligan , Bryan W. Paeper , Jeffrey Van Ness , David G. Winkler
IPC分类号： C07K1600
CPC分类号： G01N33/74 , A01K2217/05 , A01K2217/075 , A61K38/18 , A61K39/3955 , A61K45/06 , A61K48/00 , C07K14/475 , C07K14/51 , C07K16/18 , C07K16/22 , C07K2317/11 , C07K2317/23 , C07K2319/00 , C12N2799/021 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/172 , G01N33/6872 , G01N33/84 , G01N2333/47 , G01N2333/495 , G01N2333/51 , G01N2500/02 , G01N2500/20
摘要： Compositions and methods for increasing bone density using antibodies directed to a novel class or family of TGF-&bgr; binding proteins are provided. The disclosed compositions are useful, for example, in the diagnosis, prevention and/or treatment of diseases associated with a loss of bone density, for example osteoporosis.
摘要翻译：提供了使用针对TGF-β结合蛋白的新类或家族的抗体增加骨密度的组合物和方法。所公开的组合物可用于例如诊断，预防和/或治疗与骨密度丧失相关的疾病，例如骨质疏松症。

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