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1. 发明授权

US5469712A Device for dehydrating and/or embedding samples 失效
标题翻译：用于脱水和/或嵌入样品的装置
公开(公告)号：US5469712A
公开(公告)日：1995-11-28
申请号：US232152
申请日：1994-05-31
申请人： Hellmuth Sitte , Klaus Neumann , Ludwig Edelmann , Helmut Haessig , Anton Lang , Heinrich Kleber
发明人： Hellmuth Sitte , Klaus Neumann , Ludwig Edelmann , Helmut Haessig , Anton Lang , Heinrich Kleber
IPC分类号： F17C3/08 , F25D3/10 , G01N1/28 , G01N1/36 , G01N1/42 , F25B19/00
CPC分类号： F17C3/085 , F25D3/10 , G01N1/36 , G01N1/42 , F17C2203/03 , F17C2203/0636 , F17C2221/014 , F17C2223/0161
摘要： Device for dehydrating and/or embedding preferably frozen samples, comprising a Dewar vessel (1) filled with liquid nitrogen (2) and a metallic element (4), anchored to the floor (3), of material of good thermal conductivity, which exhibits at its upper end in the region of attachment of the Dewar neck a cover (5) with a metallic cooling surface (6, 7) which corresponds with the complementarily designed lower contact surfaces of the thermostatically heated (9, 10) substitution (PLT) containers (8) or respectively of the lower part of a freeze-drying chamber in a manner which ensures a good heat transfer between the corresponding surfaces.
摘要翻译： PCT No.PCT / EP93 / 02408 Sec。 371日期1994年5月31日（e）日期1994年5月31日PCT 1993年9月6日PCT。用于脱水和/或包埋优选冷冻样品的装置包括装有液氮（2）和金属元件（4）的杜瓦容器（1）锚定到地板（3）上，具有良好导热性的材料，其在其上端在杜瓦瓶颈附着的区域上展现出具有与补充对应的金属冷却表面（6,7）的盖（5）以确保相应表面之间的良好热传递的方式设计恒温加热（9,10）取代（PLT）容器（8）或冷冻干燥室下部的下部接触表面。

2. 发明授权

US5438838A Chamber for freeze-drying by cryosorption 失效
标题翻译：冷冻干燥冷冻室
公开(公告)号：US5438838A
公开(公告)日：1995-08-08
申请号：US232151
申请日：1994-06-01
申请人： Hellmuth Sitte , Klaus Neumann , Ludwig Edelmann , Helmut Haessig , Heinrich Kleber
发明人： Hellmuth Sitte , Klaus Neumann , Ludwig Edelmann , Helmut Haessig , Heinrich Kleber
IPC分类号： F17C3/08 , F25D3/10 , F26B5/06 , G01N1/28 , G01N1/42 , F04B37/08
CPC分类号： F17C3/085 , F25D3/10 , F26B5/06 , G01N1/42
摘要： A chamber system for cryosorption freeze-drying of biological specimens in a Dewar vessel, comprises a chamber having a cylindrical metal wall and fitted within the Dewar vessel, a lower outer edge of which chamber is connected in a vacuum-tight manner to a lower edge of a metallic rotational component, the metallic rotational component having a bottom contact surface, which bottom contact surface corresponds with a complementary top contact surface on top of a cylindrical body around which liquid nitrogen flows and cools the metallic rotational component, the rotational component having a chamber to receive a drying agent for conducting cryosorption, for example a molecular screen, as well as a connection to the drying chamber.The upper edge of the metal wall or a ring connected thereto in a vacuum-tight manner is situated outside the Dewar vessel and is therefore approximately at ambient temperature and has a vacuum connection for the connection of commercially available vacuum components. The opening at the top of the ring is preferably sealable in a vacuum-tight manner by a commercially available O-ring in conjunction with a cover.
摘要翻译： PCT No.PCT / EP93 / 02409 Sec。 371日期1994年6月1日 102（e）1994年6月1日PCT PCT 1993年9月6日PCT公布。出版物WO94 / 05996 日期：1994年3月17日。用于杜瓦容器中的生物样品冷冻干燥的室系统包括具有圆柱形金属壁并装配在杜瓦容器内的室，其下外边缘在真空中连接金属旋转部件具有底部接触表面，该底部接触表面对应于液体氮流动的圆筒体顶部的互补顶部接触表面，并且冷却金属旋转部件旋转部件具有用于接收用于进行低温吸附的干燥剂的室，例如分子筛，以及到干燥室的连接。金属壁或与真空密封连接的环的上边缘位于杜瓦容器的外部，因此大致处于环境温度并且具有用于连接市售真空部件的真空连接。环的顶部的开口优选地通过市售的O形环与盖结合而以真空密封的方式密封。

3. 发明授权

US06178757B2 Cooling chamber temperature control device 有权
标题翻译：冷却室温度控制装置
公开(公告)号：US06178757B2
公开(公告)日：2001-01-30
申请号：US09387559
申请日：1999-09-01
申请人： Hellmuth Sitte , Klaus Neumann , Helmut Haessig
发明人： Hellmuth Sitte , Klaus Neumann , Helmut Haessig
IPC分类号： G01N106
CPC分类号： G01N1/06 , F25D29/001 , G01N1/42 , G05D23/1931
摘要： A device for controlling the temperatures in a cooling chamber for a microtome, in particular an ultramicrotome, comprises at least two different temperature control circuits for controlling the temperatures of the specimen and of the cutter. The control circuit for the temperature of the specimen (8) and the control circuit for the temperature of the cutter (9), as well as, if appropriate, the control circuit for the temperature of the chamber gas, have a common setting element (17), such that the control circuit can be programmed to an identical temperature as the set value.
摘要翻译：用于控制切片机，特别是超薄切片机的冷却室中的温度的装置包括用于控制样品和切割器的温度的至少两个不同的温度控制回路。用于试样（8）的温度的控制电路和用于切割器（9）的温度的控制电路以及适当的情况下用于室内气体的温度的控制电路具有公共设定元件（ 17），使得控制电路可以被编程为与设定值相同的温度。

4. 发明授权

US4284894A Cold chamber for the working objects for microscopic and electron microscopic investigations 失效
标题翻译：用于微观和电子显微镜研究的工作对象的冷室
公开(公告)号：US4284894A
公开(公告)日：1981-08-18
申请号：US122215
申请日：1980-02-19
申请人： Hellmuth Sitte , Klaus Neumann , Heinrich Kleber , Helmut Hassig
发明人： Hellmuth Sitte , Klaus Neumann , Heinrich Kleber , Helmut Hassig
IPC分类号： A61B10/00 , F25D3/10 , G01N1/06 , G01N1/42 , G01N21/00
CPC分类号： A61B10/00 , F25D3/10 , G01N1/06 , G01N1/42 , G01N2001/068
摘要： A specimen working chamber having heat-conductive walls instead of insulative walls is disclosed.
摘要翻译：公开了一种具有导热壁而不是绝缘壁的试样工作室。

5. 发明授权

US5181443A Device for controlling the drive and forward feed of microtomes, particularly ultramicrotomes 失效
标题翻译：用于控制切片机，特别是超薄切片机的驱动和正向进给的装置
公开(公告)号：US5181443A
公开(公告)日：1993-01-26
申请号：US682955
申请日：1991-04-10
申请人： Hellmuth Sitte , Helmut Hassig , Armin Kunz , Klaus Neumann
发明人： Hellmuth Sitte , Helmut Hassig , Armin Kunz , Klaus Neumann
IPC分类号： B26D5/20 , G01N1/06
CPC分类号： G01N1/06 , B26D5/20 , G01N2001/066 , G01N2001/068 , Y10T83/141 , Y10T83/6492 , Y10T83/6566
摘要： A device for controlling the drive and forward feed of a microtome, particularly an ultramicrotome, includes a driving motor for moving a specimen or knife and a servomotor for generating a forward feed of the specimen towards an edge of the knife or vice versa. A high travelling speed of the specimen needed for rapid initial cutting is coupled to a high rate of forward feed. One single switching operation is performed, for instance, by a snap-engaging step switch via relay switches. The driving motor and the servomotor can be simultaneously switched over to pairs of values which correspond to subsequent rough cutting and ultra-thin cutting. Individual values for cutting speed and forward feed are separately preselected, readjusted, and read from a display. Selected program stages are indicated by signals.
摘要翻译：用于控制切片机，特别是超薄切片机的驱动和向前进给的装置包括用于移动样本或刀的驱动马达和用于产生朝向刀的边缘的向前进给样品的伺服电机，反之亦然。快速初始切割所需的样品的高行进速度与高速率的正向进给相结合。通过例如通过继电器开关的卡扣式步进开关来执行单个切换操作。驱动电机和伺服电动机可以同时切换成对对应于随后的粗切割和超薄切割的值。切割速度和正向进给的各个值分别被预先选择，重新调整和从显示器读取。所选程序段由信号指示。

6. 发明授权

US5226335A Automatic initial-cutting device for microtomes, particularly ultramicrotomes 失效
标题翻译：用于微型计算机的自动初始切割装置，特别是超声波
公开(公告)号：US5226335A
公开(公告)日：1993-07-13
申请号：US682954
申请日：1991-04-10
申请人： Hellmuth Sitte , Helmut Hassig , Armin Kunz , Klaus Neumann
发明人： Hellmuth Sitte , Helmut Hassig , Armin Kunz , Klaus Neumann
IPC分类号： G01N1/06
CPC分类号： G01N1/06 , G01N2001/066 , Y10T83/141 , Y10T83/148 , Y10T83/6492
摘要： A device automatically applies an object (7) to a cutting edge (11) of a knife 10), and automatically makes an initial cut in the object (7), in microtomes, especially ultramicrotomes. Force sensors (27,28), length sensors (29) or other sensors, an elecrotronic control unit (25) and an encoder (22) coupled to a drive device register forces (k,-k) connected with the separation of sections by the knife edge (11) or variations in the system triggered by these forces. Subsequently, via the electronic control unit (25), a transition from a rapid speed or rate of feed to a lower cutting speed or a lesser rate of feed for making an initial cut is automatically performed. A visual and/or acoustic signal informs the user of this change. Signals (S.sub.1, S.sub.2) from the sensors (27-29) serve for automatic adjustment of the position of two switch-over points (U.sub.1, U.sub.2) from the rapid return speed (V.sub.R) to the slower cutting movement (V.sub.S) or from this slower cutting movement to the more rapid return movement (V.sub.R). In a further development, by electronic comparison of the force/time patterns or length/time patterns, the end of the initial cutting process is established and used for a fresh automatic variation in cutting speed (V.sub.S) and rate of feed.
摘要翻译：设备自动地将物体（7）施加到刀10的切割边缘（11）上，并且在切片机（特别是超薄切片机）中自动对物体（7）进行初始切割。力传感器（27,28），长度传感器（29）或其他传感器，电子控制单元（25）和编码器（22），其耦合到与驱动装置对准的力（k，-k）刀刃（11）或由这些力触发的系统的变化。随后，通过电子控制单元（25），自动执行从快速或进给速率到较低切割速度或较小进给速度的转换，以进行初始切割。视觉和/或声音信号通知用户这种变化。来自传感器（27-29）的信号（S1，S2）用于将两个切换点（U1，U2）的位置从快速返回速度（VR）自动调整到较慢切割移动（VS）或从这种较慢的切割运动到更快速的返回运动（VR）。在进一步的发展中，通过电力比较力/时间模式或长度/时间模式，建立初始切割过程的结束，并用于切割速度（VS）和进料速率的新鲜自动变化。

7. 发明授权

US5070935A Refrigerated chamber for obtaining thin slices at low temperature 失效
标题翻译：用于在低温下获得薄片的制冷室
公开(公告)号：US5070935A
公开(公告)日：1991-12-10
申请号：US348622
申请日：1989-04-14
申请人： Hellmuth Sitte , Helmut Hassig , Klaus Neumann
发明人： Hellmuth Sitte , Helmut Hassig , Klaus Neumann
IPC分类号： F25D3/10 , G01N1/06 , G01N1/42
CPC分类号： F25D3/10 , G01N1/06 , G01N1/42
摘要： A refrigerated chamber includes, inside a metal wall and a foam insulating layer thereof, a sheet metal tank, in which, depending on levels of filling with liquid nitrogen, various compartments of the tank divided by separations, of different height are filled with LN.sub.2. Thereby, different cooling powers are available for varying temperature ranges between ambient temperature and the temperature of the liquid nitrogen. In this way one avoids an excessive consumption of nitrogen. Filling with LN.sub.2 is effected from a separating tank by means of a horizontal small-diameter pipe which during operation of the chamber is always exposed to and cooled by liquid nitrogen. Nitrogen in the form of a sweeping gas emerges in a boiling state from the tank and is guided through a cutting space (41') and can be heated by a heating plate fixed to sheet metal deflectors.
摘要翻译： PCT No.PCT / EP87 / 00596 371日期：1989年4月14日 102（e）日期1989年4月14日PCT提交1987年10月13日PCT公布。公开号WO88 / 02851 日期：1988年4月21日。冷藏室在金属壁及其泡沫绝缘层的内部包括金属板，其中根据液氮填充量的不同，通过分离分隔的罐的间隔，不同的高度用LN2填充。因此，不同的冷却功率可用于环境温度和液氮温度之间的不同温度范围。以这种方式避免了氮的过量消耗。通过水平小直径管从分离罐中进行填充LN2，在管腔的操作过程中总是暴露于液氮并由液氮冷却。清扫气体形式的氮气从罐中呈沸腾状态并被引导通过切割空间（41'），并可通过固定在金属板偏转器上的加热板进行加热。

8. 发明授权

US4745764A Method and device for metal-mirror cryofixation of biomedical or similar technical specimens 失效
标题翻译：生物医学或类似技术标本金属镜冷冻法的方法和装置
公开(公告)号：US4745764A
公开(公告)日：1988-05-24
申请号：US78304
申请日：1987-07-27
申请人： Hellmuth Sitte , Helmut Hassig , Klaus Neumann
发明人： Hellmuth Sitte , Helmut Hassig , Klaus Neumann
IPC分类号： G01N1/42 , F24F3/16
CPC分类号： G01N1/42
摘要： A method for metal-mirror cryofixation of bio-medical or similar technical specimens in which a specimen is brought into contact with a highly polished, cooled metal mirror in order to cool at least a marginal zone of the specimen suddenly to a low temperature. In order to avoid deformation of the specimen caused by freezing, provision is made, immediately after the first contact between the specimen and the metal mirror, to press the latter against one another by an additional independent force until the specimen is completely frozen. A cryofixation device for this purpose comprises a force generating device, for example, a separate power storage device (135) which, under control, functions after contact between the specimen (106) and metal mirror (102), in other words exerts the additional force between the specimen and the metal mirror (FIG. 7).
摘要翻译：一种用于生物医学或类似技术试样的金属镜冷冻法的方法，其中使试样与高度抛光的冷却金属镜接触，以便将试样的至少边缘区域突然冷却至低温。为了避免试样和金属镜之间的第一次接触之后立即进行冻结引起的样品变形，通过额外的独立力将其压靠在另一个独立的力下，直到样品完全冷冻。用于此目的的冷冻固定装置包括力产生装置，例如，单独的蓄电装置（135），其在控制下在样本（106）和金属反射镜（102）之间接触之后起作用，换句话说，施加额外的试样和金属反射镜之间的力（图7）。

9. 发明授权

US4545218A Cryogenic fixation apparatus 失效
标题翻译：低温固定仪
公开(公告)号：US4545218A
公开(公告)日：1985-10-08
申请号：US651385
申请日：1984-09-17
申请人： Klaus Neumann , Heinrich Kleber
发明人： Klaus Neumann , Heinrich Kleber
IPC分类号： G01N1/42 , G02B21/28 , F25B19/00
CPC分类号： G01N1/42 , G02B21/28
摘要： Cryogenic-fixation apparatus comprises a light source, a beam from which enter cooling liquid in a bath, through a window in the underside of the bath. The beam can be directed upwardly by means of a reflecting prism. When a lid of the bath is closed, the beam leaves the space defined by the path through an exit port in the lid, and is incident upon a specimen mounted on a specimen holder located above the exit port, the specimen holder being secured to an injector rod.
摘要翻译：低温固定装置包括光源，通过浴槽下侧的窗口从浴中进入冷却液的光束。光束可以通过反射棱镜向上引导。当关闭浴缸盖时，梁离开通过盖子出口的路径限定的空间，并且入射到安装在位于出口上方的试样架上的试样上，试样架固定在注射杆。

10. 发明申请

US20070267328A1 Process and System for Removing Contaminants from a Natural Gas Stream 审中-公开
标题翻译：从天然气流中除去污染物的过程和系统
公开(公告)号：US20070267328A1
公开(公告)日：2007-11-22
申请号：US10577200
申请日：2004-10-28
申请人： Klaus Neumann , Jayantilal Rajani
发明人： Klaus Neumann , Jayantilal Rajani
IPC分类号： C10G5/02
CPC分类号： C10L3/102 , B01D5/0054 , C10G5/02 , C10L3/10
摘要： A process and system for removing contaminants from a natural gas stream, the process comprising the steps of: (a) contacting part of the natural gas stream as a first gas stream at an elevated temperature with a first adsorbent bed in regeneration mode, to remove contaminants present on the first adsorbent bed, and to obtain a second gas stream that is enriched in contaminants compared to the first gas stream; (b) submitting the second gas stream to a gas/liquid separation step comprising cooling the second gas stream to a temperature such that at least some contaminants begin to condense into a first liquid phase that is rich in contaminants, and separating the first liquid phase from the second gas stream to create a third gas stream; wherein the gas/liquid separation step forms a first gas/liquid separation step, and wherein the process further comprises (c) submitting the third gas stream to a second gas/liquid separation step to obtain a second liquid phase that is rich in contaminants, and a lean gas stream.
摘要翻译：一种用于从天然气流中除去污染物的方法和系统，该方法包括以下步骤：（a）将处于升高温度的作为第一气流的天然气流的一部分与再生模式下的第一吸附床接触，以除去存在于第一吸附床上的污染物，并获得与第一气流相比富含污染物的第二气流; （b）将所述第二气流提供到气/液分离步骤，包括将所述第二气流冷却至至少一些污染物开始冷凝成富含污染物的第一液相，并分离所述第一液相从第二气流中产生第三气流; 其中所述气/液分离步骤形成第一气/液分离步骤，并且其中所述方法还包括（c）将所述第三气流送至第二气/液分离步骤以获得富含污染物的第二液相，和贫气流。

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