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    • 2. 发明授权
    • Method of liquid nitrogen surface vitrification
    • 液氮表面玻璃化方法
    • US08633023B2
    • 2014-01-21
    • US13818072
    • 2011-08-17
    • Fuliang DuJie XuJuan Moreno
    • Fuliang DuJie XuJuan Moreno
    • C12N5/07C12N5/073C12N5/075A01N1/02C12N5/02
    • A01N1/02A01N1/0221A01N1/0226A01N1/0236A01N1/0278A01N1/0284C12N5/0604C12N5/0609
    • A method of liquid nitrogen surface vitrification requiring an embryo washed in a rinsing medium, then incubated in a base medium and incubated in a hold medium before being washed in a vitrification medium and produced into a vitrification droplet (270). For forming the droplet, vitrification medium (210), an intermediary fluid such as air, followed by vitrification medium containing at least one embryo (250) are aspirated into the channel. The vitrification droplet consequently can contain an air bubble (220). The vitrification droplet can be produced from an instrument with a channel and dropped directly into liquid phase nitrogen producing a vitrified droplet. The vitrified droplet can be stored in cryo-vessels, and warmed for revitalization of biological function of vitrified biological cell mass or tissues, such as oocytes and/or embryos.
    • 一种液氮表面玻璃化的方法,其需要在漂洗介质中洗涤胚胎,然后在基础培养基中温育并在保持培养基中孵育,然后在玻璃化培养基中洗涤并生成玻璃化液滴(270)。 为了形成液滴,玻璃化介质(210),诸如空气的中间流体,随后含有至少一个胚胎(250)的玻璃化介质被吸入通道中。 玻璃化微滴因此可以包含气泡(220)。 玻璃化液滴可以由具有通道的仪器产生并直接滴入液相氮中,产生玻璃化液滴。 玻璃化液滴可以储存在低温容器中,并加热使玻璃化生物细胞团或组织如卵母细胞和/或胚胎的生物功能恢复活力。
    • 3. 发明申请
    • METHOD OF LIQUID NITROGEN SURFACE VITRIFICATION
    • 液氮表面维修方法
    • US20130157362A1
    • 2013-06-20
    • US13818072
    • 2011-08-17
    • Fuliang DuJie XuJuan Moreno
    • Fuliang DuJie XuJuan Moreno
    • C12N5/02
    • A01N1/02A01N1/0221A01N1/0226A01N1/0236A01N1/0278A01N1/0284C12N5/0604C12N5/0609
    • A method of liquid nitrogen surface vitrification requiring an embryo washed in a rinsing medium, then incubated in a base medium and incubated in a hold medium before being washed in a vitrification medium and produced into a vitrification droplet (270). For forming the droplet, vitrification medium (210), an intermediary fluid such as air, followed by vitrification medium containing at least one embryo (250) are aspirated into the channel. The vitrification droplet consequently can contain an air bubble (220). The vitrification droplet can be produced from an instrument with a channel and dropped directly into liquid phase nitrogen producing a vitrified droplet. The vitrified droplet can be stored in cryo-vessels, and warmed for revitalization of biological function of vitrified biological cell mass or tissues, such as oocytes and/or embryos.
    • 一种液氮表面玻璃化的方法,其需要在漂洗介质中洗涤胚胎,然后在基础培养基中温育并在保持培养基中孵育,然后在玻璃化培养基中洗涤并生成玻璃化液滴(270)。 为了形成液滴,玻璃化介质(210),诸如空气的中间流体,随后含有至少一个胚胎(250)的玻璃化介质被吸入通道中。 玻璃化微滴因此可以包含气泡(220)。 玻璃化液滴可以由具有通道的仪器产生并直接滴入液相氮中,产生玻璃化液滴。 玻璃化液滴可以储存在低温容器中,并加热使玻璃化生物细胞团或组织如卵母细胞和/或胚胎的生物功能恢复活力。
    • 4. 发明申请
    • COMPOSITIONS AND METHODS FOR IMPROVING THE QUALITY OF PROCESSED SPERM
    • 用于改善加工质量的组合物和方法
    • US20130183656A1
    • 2013-07-18
    • US13823843
    • 2012-06-01
    • Richard LenzJuan MorenoRamakrishnan Vishwanath
    • Richard LenzJuan MorenoRamakrishnan Vishwanath
    • A01N1/02
    • C12N5/0609A01N1/0226A01N1/0284C12N5/061C12N5/0612C12N2500/30C12N2500/38C12N2502/04G01N15/1459G01N2015/149
    • The present invention relates to compositions and methods for the handling of processed sperm including samples that are freshly collected, those transported as fresh samples, samples that are frozen and thawed, those sorted into one or more subpopulations, and those that are otherwise processed or handled that impose trauma on the cell. Trauma can reduce the motility, fertility, viability and overall integrity of the sperm and reduce the ability to fertilize, produce an embryo and a healthy offspring. The present invention relates to novel compounds that can be added to the sperm cell sample to reduce the traumatic effects of physical stress during mild as well as extensive sperm cell processing, methods of using the compounds in standard sperm processing procedures, the end products made from these methods including sperm and embryos, as well as methods of using those end products in assisted reproductive biology techniques in animals.
    • 本发明涉及用于处理加工精子的组合物和方法,包括新鲜收集的样品,作为新鲜样品运输的样品,被冷冻和解冻的样品,分为一个或多个亚种群的样品,以及另外处理或处理的样品 对细胞造成创伤。 创伤可以降低精子的活力,生育力,生存力和整体完整性,降低受精,产生胚胎和健康后代的能力。 本发明涉及可以添加到精子细胞样品中以减轻轻度和广泛精子细胞处理期间物理应激的创伤作用的新型化合物,在标准精子加工程序中使用化合物的方法,由 这些方法包括精子和胚胎,以及在动物的辅助生殖生物学技术中使用这些终产物的方法。