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    • 1. 发明授权
    • Servo-drive for rotary angle adjustment of an actuator for controlling
an opening cross-section of a medium flow line
    • 伺服驱动器,用于用于控制介质流动管线的开口横截面的致动器的旋转角度调节
    • US5554901A
    • 1996-09-10
    • US211612
    • 1994-04-11
    • Friedrich WendelManfred FranzJohannes MeiwesUwe HammerMichael AltmannDieter Dick
    • Friedrich WendelManfred FranzJohannes MeiwesUwe HammerMichael AltmannDieter Dick
    • H02K21/12F02M3/06F02M3/07H02K21/14F16K31/02H02K1/12
    • F02M3/07F02M2003/067
    • A known servo-drive including a stator which is formed with two-component design or the exciter winding has to be attached directly to the stator, which is associated with a costly production method. The new servo-drive is to permit easy and cost-effective manufacture with favorable actuation properties. The stator of the servo-drive includes in the region of the poles, weak points which are formed either by recesses or by gaps. An induction core includes a winding which is inserted into a yoke constructed on the stator by which a magnetic field can be induced in the stator. The alternating combination of sheet-metal laminas having gaps and recesses permits the torque/rotary angle characteristic of the servo-drive to be optimized with favorable production properties. The servo-drive for rotary angle adjustment of an actuator is used for example to control the opening cross-section of a medium-conducting flow line in internal combustion engines for the purpose of controlling idling speed.
    • PCT No.PCT / DE93 / 00630 Sec。 371日期1994年04月11日 102(e)日期1994年4月11日PCT提交1993年7月17日PCT公布。 出版物WO94 / 04807 日期1994年3月3日包括定子的已知的伺服驱动器,其形成为双组分设计或激励器绕组必须直接附接到定子,这与昂贵的生产方法相关联。 新的伺服驱动器允许容易且具有成本效益的制造,具有良好的致动性能。 伺服驱动器的定子在极的区域中包括通过凹部或间隙形成的弱点。 感应铁心包括一个绕组,其被插入到构造在定子上的轭中,通过该磁轭可以在定子中感应出磁场。 具有间隙和凹槽的片状金属薄片的交替组合允许伺服驱动器的扭矩/旋转角特性被优化并具有良好的生产性能。 用于致动器的旋转角度调节的伺服驱动器例如用于控制内燃机中的中导流线的开口横截面,以便控制怠速。
    • 10. 发明授权
    • Bifunctional protein for the isolation of capped mRNA
    • 双功能蛋白用于分离上限mRNA
    • US5219989A
    • 1993-06-15
    • US840565
    • 1992-02-24
    • Nahum SonenbergIsaac EderyMichael Altmann
    • Nahum SonenbergIsaac EderyMichael Altmann
    • C07K14/31C07K14/395C12N15/10
    • C12N15/1096C07K14/31C07K14/395C07K2319/705
    • A protein comprising at least a first functional site having the ability to bind the cap structure of mRNA and a second functional site having the ability to bind a solid support matrix in such a manner as to allow the first functional site to be immobilized and still remain functionally accessible to interact with the cap structure of mRNA. Also within the scope of the present invention is a method for generating a cDNA library mostly containing full-length cDNAs. The method comprises the incubation of a mixture comprising mRNA:cDNA hybrids with 1) a single strand RNA specific nuclease and 2) the above-mentioned protein. The resulting mixture is then passed through a column comprising a support matrix having the ability to bind the second functional site of the above-mentioned protein in order to selectively bind complete mRNA:cDNA hybrids. The mRNA:cDNA hybrids are then competitively eluted with a cap analog and full-length cDNA strands are separated and recovered. The present invention also includes a method for purifying capped mRNA using the above-mentioned protein. The process comprises the incubation of a mixture containing mRNA with the above-mentioned protein, passing the resulting mixture through a column comprising the support matrix having the ability to bind to the second functional site of the above-mentioned protein in order to selectively bind capped mRNAs, and competitively eluting the capped mRNAs with a cap analog.
    • 包含至少具有结合mRNA的帽结构的能力的第一功能部位的蛋白质和具有结合固体支持体基质的能力的第二功能部位,以允许第一功能部位被固定并仍然保持 功能上可与mRNA的帽结构相互作用。 也在本发明范围内的是产生大部分含有全长cDNA的cDNA文库的方法。 该方法包括将包含mRNA:cDNA杂交体的混合物与1)单链RNA特异性核酸酶和2)上述蛋白质的孵育。 然后将所得混合物通过包含具有结合上述蛋白质的第二功能位点的能力的载体基质的柱,以便选择性地结合完整的mRNA:cDNA杂交体。 然后用盖类似物竞争性地洗脱mRNA:cDNA杂交体,并分离并回收全长cDNA链。 本发明还包括使用上述蛋白质纯化封闭mRNA的方法。 该方法包括将含有mRNA的混合物与上述蛋白质孵育,使所得混合物通过包含能够结合上述蛋白质的第二功能位点的载体基质的柱,以选择性地结合封端 mRNA,并用帽类似物竞争性洗脱加盖的mRNA。