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    • 3. 发明授权
    • CLM/ECL clock phase shifter with CMOS digital control
    • 具有CMOS数字控制的CLM / ECL时钟移相器
    • US5945860A
    • 1999-08-31
    • US583169
    • 1996-01-04
    • Bernard GuayMichael Altmann
    • Bernard GuayMichael Altmann
    • H03C1/54H03K5/151H03K5/13H03K3/00
    • H03C1/542H03K5/151
    • A CML/ECL clock phase shifter device provides a 360.degree. phase control range and, upon being provided with two CML clock signals related by a known phase difference, the device produces any desired phase in response to a control signal. The device uses a CMOS current switch which generates current signals having the amplitude adjustable with the control signal, which is a digital word. Differential pairs provide amplitude modulated current signals for the input clock and the variant of the input clock. Two MOS transmission networks selectively invert each amplitude modulated signal and sum the signals from each side on a load network. The phase control resolution is optimal over four quadrants for quadrature input clock signals.
    • CML / ECL时钟移相器装置提供360°相位控制范围,并且在被提供有与已知相位差相关的两个CML时钟信号时,该装置响应于控制信号产生任何期望的相位。 该器件使用CMOS电流开关,其产生具有作为数字字的控制信号可调幅度的电流信号。 差分对为输入时钟和输入时钟的变体提供幅度调制电流信号。 两个MOS传输网络选择性地反转每个幅度调制信号,并且在负载网络上对来自每一侧的信号求和。 正交输入时钟信号的四个象限的相位控制分辨率是最佳的。
    • 10. 发明授权
    • Bifunctional protein for the isolation of capped mRNA
    • 双功能蛋白用于分离上限mRNA
    • US5219989A
    • 1993-06-15
    • US840565
    • 1992-02-24
    • Nahum SonenbergIsaac EderyMichael Altmann
    • Nahum SonenbergIsaac EderyMichael Altmann
    • C07K14/31C07K14/395C12N15/10
    • C12N15/1096C07K14/31C07K14/395C07K2319/705
    • A protein comprising at least a first functional site having the ability to bind the cap structure of mRNA and a second functional site having the ability to bind a solid support matrix in such a manner as to allow the first functional site to be immobilized and still remain functionally accessible to interact with the cap structure of mRNA. Also within the scope of the present invention is a method for generating a cDNA library mostly containing full-length cDNAs. The method comprises the incubation of a mixture comprising mRNA:cDNA hybrids with 1) a single strand RNA specific nuclease and 2) the above-mentioned protein. The resulting mixture is then passed through a column comprising a support matrix having the ability to bind the second functional site of the above-mentioned protein in order to selectively bind complete mRNA:cDNA hybrids. The mRNA:cDNA hybrids are then competitively eluted with a cap analog and full-length cDNA strands are separated and recovered. The present invention also includes a method for purifying capped mRNA using the above-mentioned protein. The process comprises the incubation of a mixture containing mRNA with the above-mentioned protein, passing the resulting mixture through a column comprising the support matrix having the ability to bind to the second functional site of the above-mentioned protein in order to selectively bind capped mRNAs, and competitively eluting the capped mRNAs with a cap analog.
    • 包含至少具有结合mRNA的帽结构的能力的第一功能部位的蛋白质和具有结合固体支持体基质的能力的第二功能部位,以允许第一功能部位被固定并仍然保持 功能上可与mRNA的帽结构相互作用。 也在本发明范围内的是产生大部分含有全长cDNA的cDNA文库的方法。 该方法包括将包含mRNA:cDNA杂交体的混合物与1)单链RNA特异性核酸酶和2)上述蛋白质的孵育。 然后将所得混合物通过包含具有结合上述蛋白质的第二功能位点的能力的载体基质的柱,以便选择性地结合完整的mRNA:cDNA杂交体。 然后用盖类似物竞争性地洗脱mRNA:cDNA杂交体,并分离并回收全长cDNA链。 本发明还包括使用上述蛋白质纯化封闭mRNA的方法。 该方法包括将含有mRNA的混合物与上述蛋白质孵育,使所得混合物通过包含能够结合上述蛋白质的第二功能位点的载体基质的柱,以选择性地结合封端 mRNA,并用帽类似物竞争性洗脱加盖的mRNA。