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1. 发明授权

US5871946A Method for determining activity of enzymes in metabolically active whole cells 失效
标题翻译：测定代谢活性全细胞中酶活性的方法
公开(公告)号：US5871946A
公开(公告)日：1999-02-16
申请号：US444056
申请日：1995-05-18
申请人： Frank J. Lucas , Gerald E. Jaffe , Steven Bott , James H. Carter
发明人： Frank J. Lucas , Gerald E. Jaffe , Steven Bott , James H. Carter
IPC分类号： C12Q1/34 , G01N33/574 , C12Q1/02
CPC分类号： C12Q1/34 , G01N33/574 , Y10S435/962 , Y10S435/968
摘要： An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.
摘要翻译：公开了一种用于测定代谢活性整个细胞内的酶的活性的测定化合物或其盐。测定化合物包括离去基团和指示剂组。离去基团选自包括氨基酸，肽，糖，硫酸盐，磷酸盐，酯，磷酸酯，核苷酸，多核苷酸，核酸，嘧啶，嘌呤，核苷，脂质及其混合物的组。指示基团选自与离去基团连接时具有第一状态的化合物，当离去基团被酶从指示剂基团切割时的第二种状态。优选地，指示剂化合物是这些化合物的罗丹明110，rhodol和荧光素以及类似物。还公开了合成化合物的方法以及使用这些化合物测定酶活性的方法。

2. 发明授权

US5776720A Assay reagent 失效
标题翻译：分析试剂
公开(公告)号：US5776720A
公开(公告)日：1998-07-07
申请号：US443776
申请日：1995-05-18
申请人： Gerald E. Jaffe , Frank J. Lucas , James H. Carter
发明人： Gerald E. Jaffe , Frank J. Lucas , James H. Carter
IPC分类号： C12Q1/02 , C12Q1/34 , G01N33/574 , C12Q1/00
CPC分类号： C12Q1/34 , C12Q1/02 , G01N33/574 , C12Q2334/40 , C12Q2337/40
摘要： An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.
摘要翻译：公开了一种用于测定代谢活性整个细胞内的酶的活性的测定化合物或其盐。测定化合物包括离去基团和指示剂组。离去基团选自包括氨基酸，肽，糖，硫酸盐，磷酸盐，酯，磷酸酯，核苷酸，多核苷酸，核酸，嘧啶，嘌呤，核苷，脂质及其混合物的组。指示基团选自与离去基团连接时具有第一状态的化合物，当离去基团被酶从指示剂基团切割时的第二种状态。优选地，指示剂化合物是这些化合物的罗丹明110，rhodol和荧光素以及类似物。还公开了合成化合物的方法以及使用这些化合物测定酶活性的方法。

3. 发明授权

US5733719A Method of making an assay compound 失效
标题翻译：制备测定化合物的方法
公开(公告)号：US5733719A
公开(公告)日：1998-03-31
申请号：US445217
申请日：1995-05-18
申请人： Gerald E. Jaffe , Frank J. Lucas , James H. Carter
发明人： Gerald E. Jaffe , Frank J. Lucas , James H. Carter
IPC分类号： C12Q1/34 , G01N33/574 , C12Q1/00 , C12Q1/02
CPC分类号： C12Q1/34 , G01N33/574 , Y10S435/968
摘要： An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.
摘要翻译：公开了一种用于测定代谢活性整个细胞内的酶的活性的测定化合物或其盐。测定化合物包括离去基团和指示剂组。离去基团选自包括氨基酸，肽，糖，硫酸盐，磷酸盐，酯，磷酸酯，核苷酸，多核苷酸，核酸，嘧啶，嘌呤，核苷，脂质及其混合物的组。指示基团选自与离去基团连接时具有第一状态的化合物，当离去基团被酶从指示剂基团切割时的第二种状态。优选地，指示剂化合物是这些化合物的罗丹明110，rhodol和荧光素以及类似物。还公开了合成化合物的方法以及使用这些化合物测定酶活性的方法。

4. 发明授权

US5849513A Assay reagent 失效
公开(公告)号：US5849513A
公开(公告)日：1998-12-15
申请号：US904400
申请日：1997-07-31
申请人： Gerald E. Jaffe , Frank J. Lucas , James H. Carter
发明人： Gerald E. Jaffe , Frank J. Lucas , James H. Carter
IPC分类号： C12Q1/02 , C12Q1/34 , G01N33/574 , C12Q1/00
CPC分类号： C12Q1/34 , C12Q1/02 , G01N33/574 , C12Q2334/40 , C12Q2337/40
摘要： An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.

5. 发明授权

US5698411A Method for determining activity of enzymes in metabolically active whole cells 失效
标题翻译：测定代谢活性全细胞中酶活性的方法
公开(公告)号：US5698411A
公开(公告)日：1997-12-16
申请号：US444051
申请日：1995-05-18
申请人： Frank J. Lucas , Gerald E. Jaffe , Steven E. Bott , James H. Carter
发明人： Frank J. Lucas , Gerald E. Jaffe , Steven E. Bott , James H. Carter
IPC分类号： C12Q1/26 , C12Q1/34 , C12Q1/37 , G01N33/574 , G01N33/58 , C12Q1/02 , C12Q1/00
CPC分类号： G01N33/574 , C12Q1/26 , C12Q1/34 , C12Q1/37 , G01N33/582 , G01N2333/95 , G01N2333/96413 , G01N2333/96466 , G01N2333/96469 , G01N2800/52
摘要： A method for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound is also disclosed.
摘要翻译：公开了一种用于测定代谢活性全细胞内酶的活性的方法。测定化合物包括离去基团和指示剂组。离去基团选自包括氨基酸，肽，糖，硫酸盐，磷酸盐，酯，磷酸酯，核苷酸，多核苷酸，核酸，嘧啶，嘌呤，核苷，脂质及其混合物的组。指示基团选自与离去基团连接时具有第一状态的化合物，当离去基团被酶从指示剂基团切割时的第二种状态。优选地，指示剂化合物是这些化合物的罗丹明110，rhodol和荧光素以及类似物。还公开了合成该化合物的方法。

6. 发明授权

US08017048B1 Emergency shelter and associated methods 有权
标题翻译：紧急避难所及相关方法
公开(公告)号：US08017048B1
公开(公告)日：2011-09-13
申请号：US11682029
申请日：2007-03-05
申请人： James H. Carter , James W. Barnwell
发明人： James H. Carter , James W. Barnwell
IPC分类号： E04B1/16
CPC分类号： E04B1/3211 , B29C44/022 , B29C44/367 , B29K2075/00 , B29L2031/776 , E04B2001/3229 , E04B2001/3264
摘要： An emergency shelter includes a domed foam structure that is constructed on-site or at a remote location from foam that can be mixed on-site. The structure can be made on-site by spraying foam in a flowable state in a predetermined pattern to build up walls to form a dome. The foam can be sprayed, for example, in a substantially helical pattern from a centrally located spray nozzle that is rotated to deposit a finite-thickness increment of foam over a time period sufficient that, by the time the nozzle reaches a previously sprayed area, the foam already deposited has had time to cure.
摘要翻译：紧急避难所包括一个穹顶泡沫结构，该结构在现场或远程位置构建，可以在现场进行混合。可以通过以预定图案以可流动状态喷射泡沫以建立壁以形成圆顶而在现场制造该结构。泡沫可以例如以基本上螺旋形的方式从位于中心的喷嘴喷射，该喷嘴在一段时间内旋转以沉积有限厚度的泡沫增量，足以在喷嘴到达预先喷射的区域时，已经存放的泡沫已经有时间治愈了。

7. 发明授权

US4528274A Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes 失效
标题翻译：多用途血液稀释液和裂解剂用于差异测定白细胞淋巴样骨髓细胞群体
公开(公告)号：US4528274A
公开(公告)日：1985-07-09
申请号：US478960
申请日：1983-03-25
申请人： James H. Carter , Stephen L. Ledis , Harold R. Crews , Ted Sena , Fred L. Larsen
发明人： James H. Carter , Stephen L. Ledis , Harold R. Crews , Ted Sena , Fred L. Larsen
IPC分类号： G01N33/49 , G01N15/12 , G01N33/50 , G01N33/48 , C09K3/00
CPC分类号： G01N33/5094 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A method and reagent system is described for differential determination of more that one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolyzing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolyzing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is a non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolyzation.
摘要翻译：描述了一种方法和试剂系统，用于使用自动血细胞分析仪差异确定一类白细胞群体。试剂系统包括血液稀释剂和基质分解试剂。血液稀释剂是用于维持红细胞形态，血细胞稳定，缓冲和抑菌作用的成分的渗透平衡水溶液。基质分解试剂是季铵盐的水溶液，着色剂形成剂和作为非阳离子表面活性剂的添加剂的混合物。具有添加剂的季铵盐用于相对于彼此和体积参考点，淋巴和骨髓群体的定位的目的。添加剂还用于防止或减少分析仪的感测孔中的蛋白质沉积物的量，该沉积物倾向于从血细胞层析产生的细胞碎片积聚。

8. 发明授权

US4495848A Pyro-gun 失效
标题翻译：火焰枪
公开(公告)号：US4495848A
公开(公告)日：1985-01-29
申请号：US285174
申请日：1981-07-06
申请人： Alexander G. Rozner , Daniel S. Lenko , James H. Carter
发明人： Alexander G. Rozner , Daniel S. Lenko , James H. Carter
IPC分类号： F41A19/58 , B23K7/10 , F23Q7/12
CPC分类号： F41H9/02 , F41A19/58 , F41C7/00 , F41C9/00
摘要： A hand held, shoulder mounted gun with time delay circuitry means to safely ignite a powdered pyrotechnic material.
摘要翻译：带有延时电路的手持式肩式枪装置意味着安全地点燃烟火材料。

9. 发明授权

US5686308A Reagent and method for differential determination of leukocytes in blood 失效
标题翻译：用于血液中白细胞差异测定的试剂和方法
公开(公告)号：US5686308A
公开(公告)日：1997-11-11
申请号：US488630
申请日：1995-06-08
申请人： Yi Li , Carole Young , Timothy J. Fischer , James H. Carter
发明人： Yi Li , Carole Young , Timothy J. Fischer , James H. Carter
IPC分类号： G01N33/48 , G01N33/49 , G01N33/50 , G01N33/569 , G01N33/72 , G01N31/00
CPC分类号： G01N33/5094 , G01N33/56972 , G01N33/721 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enable the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system find use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.
摘要翻译：提供溶解试剂组合物，其选择性地在血液样品中进行红细胞分解。此外，提供了使得能够分化白细胞的至少两个亚群的溶解试剂系统。还提供了使用溶解试剂系统的方法。此外，溶解试剂系统可用于测定血液中的血红蛋白。裂解试剂系统利用具有酸和高渗，碱性稳定剂的乙氧基化长链化合物裂解试剂。系统和分析方法维持白细胞的细胞形态，可用于分析正常和异常血液样本，新鲜和老化血液，人和非人动物血液样品以及其他流体样品如骨髓。

10. 发明授权

US4346018A Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes 失效
标题翻译：多用途血液稀释液和裂解剂用于差异测定白细胞淋巴样骨髓细胞群体
公开(公告)号：US4346018A
公开(公告)日：1982-08-24
申请号：US159782
申请日：1980-06-16
申请人： James H. Carter , Stephen L. Ledis , Harold R. Crews
发明人： James H. Carter , Stephen L. Ledis , Harold R. Crews
IPC分类号： G01N33/48 , G01N15/12 , G01N33/49 , G01N33/50 , G01N33/72
CPC分类号： G01N33/721 , G01N33/5094 , G01N2015/0069 , Y10T436/107497
摘要： An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, dimethylolurea and chlorhexidene diacetate which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.
摘要翻译：一种等渗多用途血液稀释剂，以及一种使用该稀释液与弱溶解试剂系统的方法，该方法特别适用于常规计数传统的血红蛋白值，以及白细胞淋巴样骨髓细胞的测定，特别是在自动粒子计数系统。该血液稀释剂能够提供准确，可重复的测试结果。它是含有普鲁卡因盐酸盐的预选pH的渗透平衡水溶液，用于在手术期间保持红细胞形态，作为血细胞稳定剂的N-（2-乙酰氨基）亚氨基二乙酸（ADA））和抑菌剂包括1-羟基吡啶-2- 硫酮，二羟甲基脲和氯己酮二乙酸酯，其与ADA一起允许优先测定骨髓淋巴样白血球和其他血液学值。裂解剂是具有表面活性的至少一种季铵盐的水溶液和碱金属氰化物的混合物。

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