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1. 发明授权

US4528274A Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes 失效
标题翻译：多用途血液稀释液和裂解剂用于差异测定白细胞淋巴样骨髓细胞群体
公开(公告)号：US4528274A
公开(公告)日：1985-07-09
申请号：US478960
申请日：1983-03-25
申请人： James H. Carter , Stephen L. Ledis , Harold R. Crews , Ted Sena , Fred L. Larsen
发明人： James H. Carter , Stephen L. Ledis , Harold R. Crews , Ted Sena , Fred L. Larsen
IPC分类号： G01N33/49 , G01N15/12 , G01N33/50 , G01N33/48 , C09K3/00
CPC分类号： G01N33/5094 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A method and reagent system is described for differential determination of more that one class of leukocyte populations, using an automatic blood cell analyzer. The reagent system includes a blood diluent and stromatolyzing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The stromatolyzing reagent is a mixture of an aqueous solution of quaternary ammonium salts, a chromagen forming agent and an additive which is a non-cationic surfactant. The quaternary ammonium salts, with the additive, serve the purpose of positioning, relative to one another and a volume reference point, the lymphoid and myeloid populations. The additive also acts to prevent or to reduce the amount of protein deposit in the sensing orifices of the analyzer, which deposit tends to accumulate from the cell debris resulting from blood cell stromatolyzation.
摘要翻译：描述了一种方法和试剂系统，用于使用自动血细胞分析仪差异确定一类白细胞群体。试剂系统包括血液稀释剂和基质分解试剂。血液稀释剂是用于维持红细胞形态，血细胞稳定，缓冲和抑菌作用的成分的渗透平衡水溶液。基质分解试剂是季铵盐的水溶液，着色剂形成剂和作为非阳离子表面活性剂的添加剂的混合物。具有添加剂的季铵盐用于相对于彼此和体积参考点，淋巴和骨髓群体的定位的目的。添加剂还用于防止或减少分析仪的感测孔中的蛋白质沉积物的量，该沉积物倾向于从血细胞层析产生的细胞碎片积聚。

2. 发明授权

US4485175A Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes 失效
标题翻译：三体积微分测定白细胞淋巴细胞，单核细胞和粒细胞群体的方法
公开(公告)号：US4485175A
公开(公告)日：1984-11-27
申请号：US454926
申请日：1983-01-03
申请人： Stephen L. Ledis , Ted Sena , Harold R. Crews , James H. Carter, II
发明人： Stephen L. Ledis , Ted Sena , Harold R. Crews , James H. Carter, II
IPC分类号： G01N33/48 , G01N15/12 , G01N33/483 , G01N33/49 , G01N33/50 , G01N33/72
CPC分类号： G01N33/5094 , G01N15/1209 , Y10T436/101666 , Y10T436/107497
摘要： A method and reagent system is described for differential determination of three-populations of leukocytes (lymphocyte, monocyte and granulocyte), using an automatic counting system. The reagent system includes a blood diluent and lysing reagent. The blood diluent is an osmotically balanced aqueous solution of ingredients at a preselected pH for maintaining erythrocyte morphology, blood cell stabilizing, buffering and bacteriostatic action. The lysing reagent is a mixture of an aqueous solution of quaternary ammonium salts, which lysing reagent is added to the diluted blood under more mild conditions of concentration and at a slower rate than is current practice in order to obtain an unexpected volumetric modification of at least one of the three-populations of leukocytes, whereby volumetric differential analysis can be accomplished.
摘要翻译：描述了使用自动计数系统来区分白细胞（淋巴细胞，单核细胞和粒细胞）群体的方法和试剂系统。试剂系统包括血液稀释剂和裂解试剂。血液稀释剂是预选pH值的渗透平衡的成分水溶液，用于维持红细胞形态，血细胞稳定，缓冲和抑菌作用。裂解试剂是季铵盐水溶液的混合物，其在更温和的浓度条件下以较慢的速率将裂解试剂加入到稀释的血液中，目的是为了获得至少的意外的体积改性白细胞的三个群体之一，从而可以实现体积差异分析。

3. 发明授权

US5731206A Method and reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples 失效
标题翻译：用于从全血样品中分离，鉴定和/或分析白细胞的方法和试剂系统
公开(公告)号：US5731206A
公开(公告)日：1998-03-24
申请号：US630868
申请日：1996-03-29
申请人： Stephen L. Ledis , Harold R. Crews , Timothy J. Fischer , Ted Sena
发明人： Stephen L. Ledis , Harold R. Crews , Timothy J. Fischer , Ted Sena
IPC分类号： G01N33/48 , G01N33/50 , G01N31/00
CPC分类号： G01N33/5094 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A lytic reagent composition and a kit of a lytic reagent system are disclosed for the rapid isolation, identification and/or analysis of at least five subpopulations of leukocytes from a whole blood sample. The composition and system have application to any environment in which the study and/or analysis of the leukocyte fraction of whole blood requires their isolation in their native or near native state. The lytic reagent composition of this invention comprises saponin and a carboxylic acid of the formula RCOOH wherein R is H or a C.sub.1-3 aliphatic hydrocarbon radial optionally substituted by one or more carbonyl and/or hydroxy groups. The kit of a lytic reagent system comprises the lytic reagent composition and a quench reagent.
摘要翻译：公开了溶解试剂组合物和溶解试剂体系试剂盒，用于从全血样品中快速分离，鉴定和/或分析至少五个白细胞亚群。该组合物和系统适用于任何环境，其中全血白细胞分数的研究和/或分析需要其天然或接近天然状态的分离。本发明的溶解试剂组合物包含皂角苷和式RCOOH的羧酸，其中R是H或被一个或多个羰基和/或羟基取代的C 3-脂肪烃。裂解试剂系统的试剂盒包含裂解试剂组合物和淬灭试剂。

4. 发明授权

US4751179A Method and reagents for differential determination of four populations of leukocytes in blood 失效
标题翻译：用于差异测定血液中四种白细胞数量的方法和试剂
公开(公告)号：US4751179A
公开(公告)日：1988-06-14
申请号：US615966
申请日：1984-05-31
申请人： Stephen L. Ledis , Harold R. Crews , Ted Sena
发明人： Stephen L. Ledis , Harold R. Crews , Ted Sena
IPC分类号： G01N33/49 , A01N1/02 , C12Q1/04 , C12Q1/06 , C12Q1/24 , G01N1/00 , G01N1/30 , G01N33/48 , G01N33/50
CPC分类号： G01N33/5094 , G01N33/48 , Y10T436/101666 , Y10T436/107497
摘要： This invention describes a reagent system, including saponin in a lysing reagent and a rapidly active cross-linking agent such as glutaraldehyde as a fixing reagent, which reproducably affects whole blood to cause the red blood cells to stromatolyze and modifies the leukocytes to generate data to define four distinct clusters for detection and classification by flow analysis instrumentation. The clusters represent the four major leukocyte types found in blood: lymphocytes, monocytes, neutrophils and eosinophils, thus providing a method of leukocyte differential analysis. The parameters used for the leukocyte classification include combinations of two or more of DC (Coulter) volume, high frequency (RF) size, opacity (RF size/DC volume), light scatter at various angular ranges, and fluorescence at various wavelengths of illumination.
摘要翻译：本发明描述了一种试剂系统，其包括裂解试剂中的皂角苷和作为固定剂的戊二醛的快速活性交联剂，其可全面影响全血，使红细胞进行基质分解并修饰白细胞以产生数据通过流量分析仪器定义四个不同的簇进行检测和分类。这些簇代表血液中发现的四种主要白细胞类型：淋巴细胞，单核细胞，嗜中性粒细胞和嗜酸性粒细胞，从而提供白细胞差异分析方法。用于白细胞分类的参数包括DC（Coulter）体积，高频（RF）尺寸，不透明度（RF尺寸/ DC体积），各种角度范围内的光散射和各种照明波长的荧光两种或更多种的组合。

5. 发明授权

US5155044A Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples 失效
标题翻译：用于从全血样品中分离，鉴定和/或分析白细胞的裂解试剂系统
公开(公告)号：US5155044A
公开(公告)日：1992-10-13
申请号：US808041
申请日：1991-12-12
申请人： Stephen L. Ledis , Harold R. Crews , Timothy J. Fischer , Ted Sena
发明人： Stephen L. Ledis , Harold R. Crews , Timothy J. Fischer , Ted Sena
IPC分类号： G01N33/50 , G01N33/569
CPC分类号： G01N33/5094 , G01N33/56972 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： A method and reagent system are disclosed for the rapid isolation, identification and/or analysis of leukocytes from a whole blood sample. The method and reagent system of this invention has application to any environment in which the study and/or analysis of the leukocyte fraction of whole blood requires their isolation in their native or near native state. One of the environments in which this invention can be used to advantage is in the performance of white cell differentiation on automated instrumentation designed for that purpose. In one of the preferred embodiments of this invention, the lytic reagent can contain a mixture of both formic and acetic acid, with the formic acid comprising the major component thereof and the acetic acid being present in only minor quantities, (if at all). This reagent system is used to selectively effect stromatolysis of red blood cells and create subtle modifications to the leukocyte population to enable their automated differentiation into five (5) sub-populations. The advantage and uniqueness of this reagent system is the surprising speed at which it is able to effect the foregoing objectives (generally less than 10 seconds at room temperature) and the ability to further differentiate the leukocyte population (notably, the granulocyte population). Following stromatolysis, a quenching agent is added to retard the reactivity of the lytic reagent system and, thus, inhibit any further dramatic changes to the leukocyte population. The treatment of the whole blood sample in the foregoing manner is further unique in that the leukocyte fraction of the sample has retained its characteristic immunochemical response.
摘要翻译：公开了用于从全血样品快速分离，鉴定和/或分析白细胞的方法和试剂系统。本发明的方法和试剂系统适用于其中全血白细胞分数的研究和/或分析需要在其天然或接近天然状态下分离的任何环境。本发明可用于有利的环境之一是在为此设计的自动化仪器上实现白细胞分化。在本发明的一个优选实施方案中，溶解剂可以含有甲酸和乙酸的混合物，其中甲酸包含其主要组分，乙酸仅以少量存在（如果有的话）。该试剂系统用于选择性地影响红细胞的间质分解，并对白细胞群体产生微妙的修饰，使其能够自动分化为五（5）个亚种群。该试剂系统的优点和独特之处在于其能够达到上述目的（通常在室温下通常小于10秒）以及进一步区分白细胞群体（特别是粒细胞群体）的能力的惊人速度。在层间裂解后，加入淬灭剂以延缓溶解试剂体系的反应性，从而抑制白细胞群体的任何进一步的剧烈变化。以上述方式对全血样品的处理是进一步的独特之处在于，样品的白细胞部分保持了其特有的免疫化学应答。

6. 发明授权

US4346018A Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes 失效
标题翻译：多用途血液稀释液和裂解剂用于差异测定白细胞淋巴样骨髓细胞群体
公开(公告)号：US4346018A
公开(公告)日：1982-08-24
申请号：US159782
申请日：1980-06-16
申请人： James H. Carter , Stephen L. Ledis , Harold R. Crews
发明人： James H. Carter , Stephen L. Ledis , Harold R. Crews
IPC分类号： G01N33/48 , G01N15/12 , G01N33/49 , G01N33/50 , G01N33/72
CPC分类号： G01N33/721 , G01N33/5094 , G01N2015/0069 , Y10T436/107497
摘要： An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, dimethylolurea and chlorhexidene diacetate which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.
摘要翻译：一种等渗多用途血液稀释剂，以及一种使用该稀释液与弱溶解试剂系统的方法，该方法特别适用于常规计数传统的血红蛋白值，以及白细胞淋巴样骨髓细胞的测定，特别是在自动粒子计数系统。该血液稀释剂能够提供准确，可重复的测试结果。它是含有普鲁卡因盐酸盐的预选pH的渗透平衡水溶液，用于在手术期间保持红细胞形态，作为血细胞稳定剂的N-（2-乙酰氨基）亚氨基二乙酸（ADA））和抑菌剂包括1-羟基吡啶-2- 硫酮，二羟甲基脲和氯己酮二乙酸酯，其与ADA一起允许优先测定骨髓淋巴样白血球和其他血液学值。裂解剂是具有表面活性的至少一种季铵盐的水溶液和碱金属氰化物的混合物。

7. 发明授权

US4962038A Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes 失效
标题翻译：多用途血液稀释液和裂解剂用于差异测定白细胞淋巴样骨髓细胞群体
公开(公告)号：US4962038A
公开(公告)日：1990-10-09
申请号：US735245
申请日：1985-05-17
申请人： James H. Carter , Stephen L. Ledis , Harold R. Crews
发明人： James H. Carter , Stephen L. Ledis , Harold R. Crews
IPC分类号： G01N15/12 , G01N33/50 , G01N33/72
CPC分类号： G01N33/5094 , G01N15/1209 , G01N33/721 , Y10T436/10 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.
摘要翻译：一种等渗多用途血液稀释剂，以及一种使用该稀释液与弱溶解试剂系统的方法，该方法特别适用于常规计数传统的血红蛋白值，以及白细胞淋巴样骨髓细胞的测定，特别是在自动粒子计数系统。该血液稀释剂能够提供准确，可重复的测试结果。它是含有普鲁卡因盐酸盐的预选pH的渗透平衡水溶液，用于在手术期间保持红细胞形态，作为血细胞稳定剂的N-（2-乙酰氨基）亚氨基二乙酸（ADA））和抑菌剂包括1-羟基吡啶-2- 硫醇和二羟甲基脲，其与ADA一起允许优先测定骨髓淋巴样白血球和其他血液学值。裂解剂是具有表面活性的至少一种季铵盐的水溶液和碱金属氰化物的混合物。

8. 发明授权

US4521518A Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes 失效
公开(公告)号：US4521518A
公开(公告)日：1985-06-04
申请号：US395530
申请日：1982-07-06
申请人： James H. Carter , Stephen L. Ledis , Harold R. Crews
发明人： James H. Carter , Stephen L. Ledis , Harold R. Crews
IPC分类号： G01N15/12 , G01N33/50 , G01N33/48 , C09K3/00
CPC分类号： G01N15/1209 , G01N33/5094 , Y10T436/101666 , Y10T436/105831 , Y10T436/106664 , Y10T436/107497 , Y10T436/108331
摘要： An isotonic multipurpose blood diluent, and a method for use of this diluent with a weak lysing reagent system which is especially suitable for routine enumeration of traditional hemogram values, and also the determination of lymphoid-myeloid populations of leukocytes, particularly in automatic particle counting systems.This blood diluent is capable of affording accurate, reproducible test results. It is an osmotically balanced aqueous solution of preselected pH containing Procaine hydrochloride for maintaining erythrocyte morphology during operation, N-(2-acetamido)iminodiacetic acid (ADA) as a blood cell stabilizing agent, and bacteriostatic agents including sodium 1-hydroxypyridine-2-thione, and dimethylolurea which, together with the ADA, allow preferential determination of myeloid-lymphoid leukocytes, and other hematological values.The lysing agent is a mixture of an aqueous solution of at least one quaternary ammonium salt having surface active properties, and an alkali metal cyanide.

9. 发明授权

US4405719A Method of stabilizing platelets for determining multiple platelet parameters in reference control and calibrator compositions; diluents therefor; and combination stabilization procedures 失效
标题翻译：在参考对照和校准品组合物中稳定血小板以确定多个血小板参数的方法; 稀释剂; 和组合稳定程序
公开(公告)号：US4405719A
公开(公告)日：1983-09-20
申请号：US268050
申请日：1981-05-29
申请人： Harold R. Crews , James H. Carter, II , Ted Sena
发明人： Harold R. Crews , James H. Carter, II , Ted Sena
IPC分类号： G01N33/49 , A01N1/02 , A61K35/14 , G01N33/96 , C09K3/00 , G01N33/48
CPC分类号： G01N33/96 , A01N1/02 , A01N1/0226 , G01N2496/05 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： This invention utilizes for stabilization of platelets a combination of iodoacetamide and an iminodiacetic acid or salt thereof, together with a compatible bacteriostatic agent, in an aqueous electrolytic solution which is maintained at a preselected range of pH and osmolality.In a preferred formulation, the stabilizing solution contains iodoacetamide, N-(2-acetamido)iminodiacetic acid and sodium penicillin. Ethylenediaminetetraacetic acid, or salts thereof, are optionally present as an additional ingredient.
摘要翻译：本发明用于在维持在pH和重量摩尔渗透压浓度的预选范围内的电解水溶液中将碘乙酰胺和亚氨基二乙酸或其盐的组合与相容的抑菌剂一起稳定化。在优选的制剂中，稳定溶液含有碘乙酰胺，N-（2-乙酰氨基）亚氨基二乙酸和青霉素钠。乙二胺四乙酸或其盐任选地作为另外的成分存在。

10. 发明授权

US4389490A Method of stabilizing platelets for determining multiple platelet parameters in reference control and calibrator compositions; and diluents thereof 失效
标题翻译：在参考对照和校准品组合物中稳定血小板以确定多个血小板参数的方法; 及其稀释剂
公开(公告)号：US4389490A
公开(公告)日：1983-06-21
申请号：US268049
申请日：1981-05-29
申请人： Harold R. Crews , James H. Carter, II , Ted Sena
发明人： Harold R. Crews , James H. Carter, II , Ted Sena
IPC分类号： G01N33/49 , A01N1/02 , A61K35/14 , G01N33/96 , G01N33/48 , C09K3/00
CPC分类号： A01N1/02 , A01N1/0226 , G01N33/96 , G01N2496/05 , Y10T436/101666 , Y10T436/107497 , Y10T436/108331
摘要： Stabilized reference control and calibrator compositions for determining multiple platelet parameters in stand alone platelet controls and whole blood reference controls are prepared from platelets stabilized with a fixative-stabilizing composition containing glutaraldehyde and a non-ionic surfactant which is a mixture of ethoxylates of certain isomeric linear alcohols.
摘要翻译：用于测定独立血小板对照和全血参照对照中的多种血小板参数的稳定参考对照和校正剂组合物由用含有戊二醛和非离子表面活性剂的固定剂稳定化组合物稳定的血小板制备，所述非离子表面活性剂是某些异构线性醇类。

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