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1. 发明申请

US20150259671A1 DEVICES AND SYSTEMS FOR ISOLATING BIOMOLECULES AND ASSOCIATED METHODS THEREOF 审中-公开
标题翻译：用于分离生物分子的装置和系统及其相关方法
公开(公告)号：US20150259671A1
公开(公告)日：2015-09-17
申请号：US13562947
申请日：2012-07-31
申请人： Christopher Michael Puleo , Ralf Lenigk , John Richard Nelson , Xiaohui Chen , Li Zhu , Erin Jean Finehout , Patrick McCoy Spooner
发明人： Christopher Michael Puleo , Ralf Lenigk , John Richard Nelson , Xiaohui Chen , Li Zhu , Erin Jean Finehout , Patrick McCoy Spooner
IPC分类号： C12N15/10
CPC分类号： C12N15/101 , B01L3/50273 , B01L3/502738 , B01L2200/0631 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2400/0418 , B01L2400/0487 , B01L2400/0683
摘要： A device, a system, a cartridge and a method for isolating biomolecules from biological materials are provided. The device comprises a substrate; a reagent storage location; and a self-rupturing component comprising a fluid and a pressure source embedded therein, wherein the substrate, the reagent storage location and the self-rupturing component are operationally coupled to each other. A system is provided, wherein the system comprises an extraction matrix, an enclosed matrix housing comprising a biological sample inlet, one or more biomolecule extraction reagents to extract biomolecules and at least one pressure source embedded therein, a fluidic extraction circuit; and a controller for activating the embedded pressure source. A method of isolating nucleic acids from biological materials is also provided.
摘要翻译：提供了一种用于从生物材料中分离生物分子的装置，系统，盒和方法。该装置包括基板; 试剂储存位置; 以及包含嵌入其中的流体和压力源的自破裂部件，其中所述基板，所述试剂储存位置和所述自破裂部件在操作上彼此耦合。提供了一种系统，其中所述系统包括提取矩阵，包括生物样品入口的封闭矩阵壳体，用于提取生物分子的一种或多种生物分子提取试剂和嵌入其中的至少一种压力源，流体萃取回路; 以及用于启动嵌入式压力源的控制器。还提供了从生物材料中分离核酸的方法。

2. 发明申请

US20140039177A1 METHODS OF ISOLATING NUCLEIC ACIDS UNDER REDUCED DEGRADATION CONDITION 审中-公开
标题翻译：在降低降解条件下分离核酸的方法
公开(公告)号：US20140039177A1
公开(公告)日：2014-02-06
申请号：US13599124
申请日：2012-08-30
申请人： John Richard Nelson , Li Zhu , Erin Jean Finehout , Xiaohui Chen , Kashan Ali Shaikh , Christopher Michael Puleo , Patrick McCoy Spooner
发明人： John Richard Nelson , Li Zhu , Erin Jean Finehout , Xiaohui Chen , Kashan Ali Shaikh , Christopher Michael Puleo , Patrick McCoy Spooner
IPC分类号： C07H21/00
CPC分类号： G01N27/44791 , C07H1/08 , C07H21/00 , C12N15/1017 , G01N27/44747
摘要： A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
摘要翻译：从生物材料中分离核酸的方法包括将生物材料应用于包含其中浸渍的一种或多种细胞裂解试剂的底物; 将液体施加到施加在基底上的生物材料; 从施加在基底上的生物材料中提取核酸; 并以基本上完整的形式收集所提取的核酸，其中所收集的核酸具有大于或等于20kb的分子量。

3. 发明申请

US20120152743A1 METHOD FOR ELECTROELUTING GENETIC MATERIAL FROM DRIED SAMPLES 审中-公开
标题翻译：从干燥样品中电化遗传材料的方法
公开(公告)号：US20120152743A1
公开(公告)日：2012-06-21
申请号：US12972236
申请日：2010-12-17
申请人： Erin Jean Finehout , John Richard Nelson , Patrick McCoy Spooner
发明人： Erin Jean Finehout , John Richard Nelson , Patrick McCoy Spooner
IPC分类号： C25B7/00
CPC分类号： C12N15/101
摘要： In accordance with the present disclosure, a method for extracting genetic material from a biological sample stored on a solid medium is provided. The method includes obtaining the solid medium, wherein the biological sample is applied on the solid medium, and the solid medium includes chemicals that lysed the biological sample and preserved the genetic material. The method also includes electroeluting the genetic material directly from the solid medium to a subsequent medium.
摘要翻译：根据本公开，提供了从存储在固体培养基上的生物样品中提取遗传物质的方法。该方法包括获得固体培养基，其中将生物样品施加在固体培养基上，固体培养基包括裂解生物样品并保存遗传物质的化学物质。该方法还包括将遗传物质直接从固体培养基中电泳至后续培养基。

4. 发明申请

US20140039172A1 DEVICES AND SYSTEMS FOR ISOLATING BIOMOLECULES AND ASSOCIATED METHODS THEREOF 有权
标题翻译：用于分离生物分子的装置和系统及其相关方法
公开(公告)号：US20140039172A1
公开(公告)日：2014-02-06
申请号：US13599131
申请日：2012-08-30
申请人： John Richard Nelson , Li Zhu , Xiaohui Chen , Christopher Michael Puleo , Patrick McCoy Spooner
发明人： John Richard Nelson , Li Zhu , Xiaohui Chen , Christopher Michael Puleo , Patrick McCoy Spooner
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , B01D11/02
CPC分类号： F04B19/006 , F04B19/04 , F04B19/20
摘要： A device, a system, and a method for isolating biomolecules from biological materials are provided. The device comprises a quartz-based solid phase extraction matrix comprising one or more reagents impregnated therein; and an electroosmotic pump (EOP) operationally coupled to the quartz-based solid phase extraction matrix to elute the nucleic acids, wherein the EOP comprises a plurality of electroosmotic membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes disposed alternatively and a plurality of electrodes comprising one or more cathodes and one or more anodes, wherein at least one cathode is disposed on one side of one of the membranes and at least one anode is disposed on another side of that membrane and at least one cathode or anode is disposed between a positive electroosmotic membrane and a negative electroosmotic membrane.
摘要翻译：提供了一种从生物材料中分离生物分子的装置，系统和方法。该装置包括石英基固相萃取基质，其包含一种或多种浸渍在其中的试剂; 和电渗泵（EOP），其可操作地耦合到所述基于石英的固相萃取基质以洗脱所述核酸，其中所述EOP包括多个电渗膜，所述电渗膜包含一个或多个正电渗透膜和一个或多个负电渗渗膜，包括一个或多个阴极和一个或多个阳极的多个电极，其中至少一个阴极设置在一个膜的一侧上，并且至少一个阳极设置在该膜的另一侧上，并且至少一个阴极或阳极设置在正电渗透膜和负电渗膜之间。

5. 发明授权

US09399986B2 Devices and systems for isolating biomolecules and associated methods thereof 有权
标题翻译：用于分离生物分子的装置和系统及其相关方法
公开(公告)号：US09399986B2
公开(公告)日：2016-07-26
申请号：US13599131
申请日：2012-08-30
申请人： John Richard Nelson , Li Zhu , Xiaohui Chen , Christopher Michael Puleo , Patrick McCoy Spooner
发明人： John Richard Nelson , Li Zhu , Xiaohui Chen , Christopher Michael Puleo , Patrick McCoy Spooner
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , B01D11/02 , F04B19/00 , F04B19/04 , F04B19/20
CPC分类号： F04B19/006 , F04B19/04 , F04B19/20
摘要： A device, a system, and a method for isolating biomolecules from biological materials are provided. The device comprises a quartz-based solid phase extraction matrix comprising one or more reagents impregnated therein; and an electroosmotic pump (EOP) operationally coupled to the quartz-based solid phase extraction matrix to elute the nucleic acids, wherein the EOP comprises a plurality of electroosmotic membranes comprising one or more positive electroosmotic membranes and one or more negative electroosmotic membranes disposed alternatively and a plurality of electrodes comprising one or more cathodes and one or more anodes, wherein at least one cathode is disposed on one side of one of the membranes and at least one anode is disposed on another side of that membrane and at least one cathode or anode is disposed between a positive electroosmotic membrane and a negative electroosmotic membrane.
摘要翻译：提供了一种从生物材料中分离生物分子的装置，系统和方法。该装置包括石英基固相萃取基质，其包含一种或多种浸渍在其中的试剂; 和电渗泵（EOP），其可操作地耦合到所述基于石英的固相萃取基质以洗脱所述核酸，其中所述EOP包括多个电渗膜，所述电渗膜包含一个或多个正电渗透膜和一个或多个负电渗渗膜，包括一个或多个阴极和一个或多个阳极的多个电极，其中至少一个阴极设置在一个膜的一侧上，并且至少一个阳极设置在该膜的另一侧上，并且至少一个阴极或阳极设置在正电渗透膜和负电渗膜之间。

6. 发明授权

US09574183B2 Method and kits for repairing nucleic acid sequences 有权
标题翻译：用于修复核酸序列的方法和试剂盒
公开(公告)号：US09574183B2
公开(公告)日：2017-02-21
申请号：US13344504
申请日：2012-01-05
申请人： John Richard Nelson , Wei Gao
发明人： John Richard Nelson , Wei Gao
IPC分类号： C12N9/12 , C12N9/00
CPC分类号： C12N9/1252 , C12N9/93
摘要： Methods and kits for DNA repair are provided. The methods and kits described herein repair multiple types of DNA damage. The kit may include a plurality of enzymes to repair a greater variety of lesions than any single enzyme is capable of repairing. Repair of damaged DNA may include releasing damaged bases from the DNA strand, nicking the DNA at the damaged sites, translating the nicks via 5′-3′ exonuclease activity, and sealing the nicks. The enzymes employed in the repair process may then be heat-inactivated, thereby obviating a purification process. The repaired DNA may then be analyzed using a variety of DNA analysis methods.
摘要翻译：提供DNA修复的方法和试剂盒。本文所述的方法和试剂盒修复多种类型的DNA损伤。试剂盒可以包括多种酶，以修复比任何单一酶能够修复的更多种病变。损伤的DNA的修复可以包括从DNA链释放受损的碱基，在受损位点切割DNA，通过5'-3'核酸外切酶活性翻译切口并密封缺口。然后可以将在修复过程中使用的酶热灭活，从而避免纯化过程。然后可以使用各种DNA分析方法分析修复的DNA。

7. 发明授权

US08921072B2 Methods to generate DNA mini-circles 有权
标题翻译：产生DNA迷你圈的方法
公开(公告)号：US08921072B2
公开(公告)日：2014-12-30
申请号：US12202644
申请日：2008-09-02
申请人： John Richard Nelson , Nichole Lea Wood , Gregory Andrew Grossmann , Robert Scott Duthie
发明人： John Richard Nelson , Nichole Lea Wood , Gregory Andrew Grossmann , Robert Scott Duthie
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6844 , A61K2039/53 , C12N15/10 , C12N15/64 , C12N2800/30 , C12Q2531/125 , C12Q2521/107
摘要： Methods and kits for generating circular nucleic acids in a cell-free system, and uses for the generated circular nucleic acids are provided. The methods comprise in vitro amplification of a nucleic acid template comprising a recombination site to produce tandem repeat nucleic acid sequence, and employ a recombination protein to generate the circular nucleic acids from the tandem repeat nucleic acid sequence.
摘要翻译：提供了在无细胞系统中产生环状核酸的方法和试剂盒，以及用于产生的环状核酸的方法和试剂盒。所述方法包括体外扩增包含重组位点以产生串联重复核酸序列的核酸模板，并使用重组蛋白从串联重复核酸序列产生环状核酸。

8. 发明授权

US08765643B2 Composition, device and associated method 有权
标题翻译：组成，装置和相关方法
公开(公告)号：US08765643B2
公开(公告)日：2014-07-01
申请号：US11680646
申请日：2007-03-01
申请人： Zhida Pan , Su Lu , Liangliang Qiang , John Yupeng Gui , John Richard Nelson , Bahram Moasser , Anup Sood , Gregory Andrew Grossmann , Wei Cai , Zhixin Zheng
发明人： Zhida Pan , Su Lu , Liangliang Qiang , John Yupeng Gui , John Richard Nelson , Bahram Moasser , Anup Sood , Gregory Andrew Grossmann , Wei Cai , Zhixin Zheng
IPC分类号： C40B40/04 , G01N33/53 , G01N33/566
CPC分类号： G01N33/58 , G01N31/22
摘要： The composition includes a first probe and a first initiator bonded to the first probe. The composition further includes a second probe and a second initiator bonded to the second probe. The first probe and the second probe are capable of binding to a single analyte. An associated kit, device, and method are provided.
摘要翻译：组合物包括第一探针和结合到第一探针的第一引发剂。组合物还包括第二探针和结合到第二探针的第二引发剂。第一个探针和第二个探针能够结合单个分析物。提供相关的套件，设备和方法。

9. 发明授权

US08741813B2 Composition, device and associated method 有权
标题翻译：组成，装置和相关方法
公开(公告)号：US08741813B2
公开(公告)日：2014-06-03
申请号：US11680597
申请日：2007-02-28
申请人： Su Lu , John Yupeng Gui , Bahram Moasser , Wei Cai , Zhixin Zheng , John Richard Nelson
发明人： Su Lu , John Yupeng Gui , Bahram Moasser , Wei Cai , Zhixin Zheng , John Richard Nelson
IPC分类号： C40B40/04 , G01N33/53 , G01N33/566
CPC分类号： C12Q1/6834 , G01N33/533 , G01N33/582
摘要： A composition includes a first probe capable of binding to a first analyte and a first initiator bonded to the first probe. The composition further includes a second probe capable of binding to a second analyte and a second initiator bonded to the second probe. At least one of the first initiator or the second initiator is capable of initiating a controlled polymerization reaction. An associated kit, device, and method are provided.
摘要翻译：组合物包括能够结合第一分析物的第一探针和与第一探针结合的第一引发剂。组合物还包括能够结合第二分析物的第二探针和与第二探针结合的第二引发剂。第一引发剂或第二引发剂中的至少一种能够引发受控的聚合反应。提供相关的套件，设备和方法。

10. 发明申请

US20140004509A1 KIT FOR ISOTHERMAL DNA AMPLIFICATION STARTING FROM AN RNA TEMPLATE 审中-公开
标题翻译：从RNA模板开始的同位素DNA扩增试剂盒
公开(公告)号：US20140004509A1
公开(公告)日：2014-01-02
申请号：US13538962
申请日：2012-06-29
申请人： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossmann , Ryan Charles Heller
发明人： John Richard Nelson , Robert Scott Duthie , Gregory Andrew Grossmann , Ryan Charles Heller
IPC分类号： C12N9/12 , G01N27/26 , G01N21/64 , C12Q1/68
CPC分类号： C12Q1/6865 , C12Q2525/117 , C12Q2545/114
摘要： A kit for amplifying deoxynucleic acid (DNA) from ribonucleic acid (RNA) template is provided. The kit for amplifying a RNA comprises at least one inosine-containing primer; and at least one enzyme comprising a reverse transcriptase activity, a strand displacement DNA polymerase activity, a nuclease acitivity for nicking DNA 3′ to an inosine residue of the primer or combinations thereof. The kit further comprises one or more quantifying reagents to detect the presence of RNA in a sample or quantify the RNA present in a sample.
摘要翻译：提供了用于从核糖核酸（RNA）模板中扩增脱氧核酸（DNA）的试剂盒。用于扩增RNA的试剂盒包含至少一种含肌苷的引物; 以及至少一种酶，其包含逆转录酶活性，链置换DNA聚合酶活性，用于将DNA 3'切割至引物的肌苷残基的核酸酶活性或其组合。试剂盒还包含一种或多种定量试剂以检测样品中RNA的存在或定量存在于样品中的RNA。

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