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1. 发明申请

US20100155244A1 INJECTION METHOD FOR MICROFLUIDIC CHIPS 有权
标题翻译：微流感注射液的注射方法
公开(公告)号：US20100155244A1
公开(公告)日：2010-06-24
申请号：US12341233
申请日：2008-12-22
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie
IPC分类号： B01D57/02
CPC分类号： B01D57/02
摘要： Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.
摘要翻译：用于在电泳期间注射样品的方法和系统，其通常包括：通过分离通道的第一端装载筛分基质; 筛分矩阵的结束距离分离通道和加载通道的交点处一定距离; 通过加载通道加载样品并填充分离通道的空的部分; 在使洗涤缓冲液流过所述装载通道的同时，在所述分离通道上施加电场; 以及将所述样品的一部分注入所述分离通道中，其中所述注入的所述样品的所述部分具有由所述筛分矩阵的末端与所述加载和分离通道的交点之间的距离确定的尺寸。

2. 发明授权

US07740747B2 Injection method for microfluidic chips 失效
标题翻译：微流控芯片注射方法
公开(公告)号：US07740747B2
公开(公告)日：2010-06-22
申请号：US11965794
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： G01N27/26
CPC分类号： G01N27/44743
摘要： A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.
摘要翻译：提供了用于毛细管电泳的微芯片。微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。微芯片还包括邻近'T'结和在分离通道上设置的第一阀和设置在'T'结处的第二阀。第二个阀是一个二通阀。将样品塞注入到“T”结和分离通道的第二端之间的区域中。

3. 发明申请

US20090071832A1 MICROFLUIDIC DEVICE WITH VERTICAL INJECTION APERTURE 有权
标题翻译：具有垂直注射孔的微流体装置
公开(公告)号：US20090071832A1
公开(公告)日：2009-03-19
申请号：US11857570
申请日：2007-09-19
申请人： Jun Xie , Shashi Thutupalli , Stacey Joy Kennerly , Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Oliver Charles Boomhower
发明人： Jun Xie , Shashi Thutupalli , Stacey Joy Kennerly , Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Oliver Charles Boomhower
IPC分类号： G01N27/26
CPC分类号： G01N27/447
摘要： A microfluidic device with a vertical injection aperture is provided. The microfluidic device comprises a separation channel, an injection aperture disposed adjacent to and in fluid communication with the separation channel. The microfluidic device further comprises a semi-permeable filter disposed adjacent to the injection aperture, wherein the filter is configured to preconcentrate a sample in the injection aperture to form a preconcentrated sample plug during an injection operation, and wherein the sample plug flows downwardly from the injection aperture to the separation channel during an electrophoresis operation.
摘要翻译：提供具有垂直注射孔的微流体装置。微流体装置包括分离通道，与分离通道相邻并且与分离通道流体连通地设置的注射孔。微流体装置还包括邻近喷射孔布置的半渗透过滤器，其中过滤器构造成在注射操作期间预浓缩注入孔中的样品以形成预浓缩样品塞，并且其中样品塞从在电泳操作期间到分离通道的注射孔径。

4. 发明申请

US20150259671A1 DEVICES AND SYSTEMS FOR ISOLATING BIOMOLECULES AND ASSOCIATED METHODS THEREOF 审中-公开
标题翻译：用于分离生物分子的装置和系统及其相关方法
公开(公告)号：US20150259671A1
公开(公告)日：2015-09-17
申请号：US13562947
申请日：2012-07-31
申请人： Christopher Michael Puleo , Ralf Lenigk , John Richard Nelson , Xiaohui Chen , Li Zhu , Erin Jean Finehout , Patrick McCoy Spooner
发明人： Christopher Michael Puleo , Ralf Lenigk , John Richard Nelson , Xiaohui Chen , Li Zhu , Erin Jean Finehout , Patrick McCoy Spooner
IPC分类号： C12N15/10
CPC分类号： C12N15/101 , B01L3/50273 , B01L3/502738 , B01L2200/0631 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2400/0418 , B01L2400/0487 , B01L2400/0683
摘要： A device, a system, a cartridge and a method for isolating biomolecules from biological materials are provided. The device comprises a substrate; a reagent storage location; and a self-rupturing component comprising a fluid and a pressure source embedded therein, wherein the substrate, the reagent storage location and the self-rupturing component are operationally coupled to each other. A system is provided, wherein the system comprises an extraction matrix, an enclosed matrix housing comprising a biological sample inlet, one or more biomolecule extraction reagents to extract biomolecules and at least one pressure source embedded therein, a fluidic extraction circuit; and a controller for activating the embedded pressure source. A method of isolating nucleic acids from biological materials is also provided.
摘要翻译：提供了一种用于从生物材料中分离生物分子的装置，系统，盒和方法。该装置包括基板; 试剂储存位置; 以及包含嵌入其中的流体和压力源的自破裂部件，其中所述基板，所述试剂储存位置和所述自破裂部件在操作上彼此耦合。提供了一种系统，其中所述系统包括提取矩阵，包括生物样品入口的封闭矩阵壳体，用于提取生物分子的一种或多种生物分子提取试剂和嵌入其中的至少一种压力源，流体萃取回路; 以及用于启动嵌入式压力源的控制器。还提供了从生物材料中分离核酸的方法。

5. 发明授权

US07927476B2 Injection method for microfluidic chips 有权
标题翻译：微流控芯片注射方法
公开(公告)号：US07927476B2
公开(公告)日：2011-04-19
申请号：US12341233
申请日：2008-12-22
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： B01D57/02
摘要： Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.
摘要翻译：用于在电泳期间注射样品的方法和系统，其通常包括：通过分离通道的第一端装载筛分基质; 筛分矩阵的结束距离分离通道和加载通道的交点处一定距离; 通过加载通道加载样品并填充分离通道的空的部分; 在使洗涤缓冲液流过所述装载通道的同时，在所述分离通道上施加电场; 以及将所述样品的一部分注入所述分离通道中，其中所述注入的所述样品的所述部分具有由所述筛分矩阵的末端与所述加载和分离通道的交点之间的距离确定的尺寸。

6. 发明申请

US20090166203A1 INJECTION METHOD FOR MICROFLUIDIC CHIPS 失效
标题翻译：微流感注射液的注射方法
公开(公告)号：US20090166203A1
公开(公告)日：2009-07-02
申请号：US11965794
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44743
摘要： A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.
摘要翻译：提供了用于毛细管电泳的微芯片。微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。所述微芯片还包括邻近所述“T”结和所述分离通道设置的第一阀，以及设置在所述“T”结处的第二阀。第二个阀是一个二通阀。将样品塞注入到“T”结和分离通道的第二端之间的区域中。

7. 发明申请

US20090166202A1 INJECTION METHOD FOR MICROFLUIDIC CHIPS 审中-公开
标题翻译：微流感注射液的注射方法
公开(公告)号：US20090166202A1
公开(公告)日：2009-07-02
申请号：US11965791
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： B01D57/02
CPC分类号： G01N27/44743 , G01N27/44791
摘要： A microchip for electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well. The injection channel and the separation channel form a ‘T’ junction. The microchip comprises a first electrode disposed at a first end of the separation channel, a second electrode disposed in front of the ‘T’ junction and adjacent to the first electrode, a third electrode disposed at a first end of the injection channel and a fourth electrode disposed at a second end of the separation channel. A portion of the sample is injected and separated into an area between the ‘T’ junction and the fourth electrode.
摘要翻译：提供用于电泳的微芯片。微芯片包括注入通道和配置成通过样品阱接收样品的分离通道。注入通道和分离通道形成“T”结。微芯片包括设置在分离通道的第一端的第一电极，设置在“T”结的前面并与第一电极相邻的第二电极，设置在注入通道的第一端的第三电极和第四电极电极设置在分离通道的第二端。样品的一部分被注入并分离成“T”结和第四电极之间的区域。

8. 发明申请

US20140039177A1 METHODS OF ISOLATING NUCLEIC ACIDS UNDER REDUCED DEGRADATION CONDITION 审中-公开
标题翻译：在降低降解条件下分离核酸的方法
公开(公告)号：US20140039177A1
公开(公告)日：2014-02-06
申请号：US13599124
申请日：2012-08-30
申请人： John Richard Nelson , Li Zhu , Erin Jean Finehout , Xiaohui Chen , Kashan Ali Shaikh , Christopher Michael Puleo , Patrick McCoy Spooner
发明人： John Richard Nelson , Li Zhu , Erin Jean Finehout , Xiaohui Chen , Kashan Ali Shaikh , Christopher Michael Puleo , Patrick McCoy Spooner
IPC分类号： C07H21/00
CPC分类号： G01N27/44791 , C07H1/08 , C07H21/00 , C12N15/1017 , G01N27/44747
摘要： A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
摘要翻译：从生物材料中分离核酸的方法包括将生物材料应用于包含其中浸渍的一种或多种细胞裂解试剂的底物; 将液体施加到施加在基底上的生物材料; 从施加在基底上的生物材料中提取核酸; 并以基本上完整的形式收集所提取的核酸，其中所收集的核酸具有大于或等于20kb的分子量。

9. 发明授权

US08377277B2 System and method for performing microfluidic manipulation 失效
标题翻译：用于执行微流体操作的系统和方法
公开(公告)号：US08377277B2
公开(公告)日：2013-02-19
申请号：US12255962
申请日：2008-10-22
申请人： Jun Xie , Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout
发明人： Jun Xie , Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout
IPC分类号： B01D57/02
CPC分类号： G01N27/44791
摘要： Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir.
摘要翻译：电泳系统和方法包括电泳装置，其中电泳包括加载通道，分离通道和注射通道。装载通道与第一和第二采样端口流体连通。分离通道连接到装载通道以形成第一交叉点，以及连接到分离通道以形成第二交叉并与第一储存器流体连通的注入通道，并且其中分离通道与第二交换流体连通水库电泳系统还包括分别耦合到第一样品端口和第一储存器的两个电极以及适于将样品移动到装载通道中以朝向第一储存器形成样品塞的第一样品端口和第二储存器在分离通道中，并且进一步将样品塞进入分离通道朝向第二储存器。

10. 发明申请

US20110132111A1 METHODS AND SYSTEMS TO PREVENT PUNCH LOSS DURING AUTOMATED SAMPLE PROCESSING 有权
标题翻译：在自动样品加工过程中防止冲击损失的方法和系统
公开(公告)号：US20110132111A1
公开(公告)日：2011-06-09
申请号：US12630899
申请日：2009-12-04
申请人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout
发明人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout
IPC分类号： G01N1/04
CPC分类号： G01N1/286 , G01N2001/288 , G01N2035/042
摘要： Automated methods and systems for punching out pieces of a porous substrate for biological samples comprising: loading the porous substrate onto a support comprising a die and an opening; moving a receptacle support in at least a z-direction to position a receptacle relative to the support so that an opening in the receptacle is aligned and substantially flush with the opening in the support; actuating a punching head so that the punching head passes through the die, thereby punching a piece out of the porous substrate; and actuating an ejector pin to eject the punched piece from the porous substrate support and into the receptacle aligned with the opening in the support.
摘要翻译：用于冲切用于生物样品的多孔基材片的自动方法和系统，包括：将所述多孔基材装载到包括模头和开口的支撑件上; 在至少z方向上移动容器支撑件以相对于支撑件定位容器，使得容器中的开口对齐并且基本上与支撑件中的开口齐平; 致动冲压头，使冲头通过冲模，从而从多孔基材上冲出一块; 并且致动顶针以将冲压件从多孔基底支撑件推出并进入与支撑件中的开口对准的容器中。

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