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1. 发明授权

US4749647A Polymerization-induced separation assay using recognition pairs 失效
标题翻译：使用识别对的聚合诱导分离测定
公开(公告)号：US4749647A
公开(公告)日：1988-06-07
申请号：US623838
申请日：1984-06-22
申请人： Elaine K. Thomas , Dennis E. Schwartz , John H. Priest , Robert C. Nowinski , Allan S. Hoffman
发明人： Elaine K. Thomas , Dennis E. Schwartz , John H. Priest , Robert C. Nowinski , Allan S. Hoffman
IPC分类号： C12Q1/68 , C12Q1/70 , G01N33/537
CPC分类号： G01N33/5375 , C12Q1/68 , C12Q1/682 , C12Q1/701 , Y10S436/827 , Y10S525/904
摘要： Methods and compounds are disclosed for determining the presence, amount of, or association between substances of interest in samples suspected of containing same. The methods are based on the polymerization-induced separation of specifically-bound, reporter-labeled recognition reactants from free, reporter-labeled recognition reactants. The methods described are applicable to any substance for which suitable recognition reactants exist or can be made and are not limited by considerations such as chemical composition or molecular size.
摘要翻译：公开了用于确定怀疑含有其的样品中感兴趣的物质的存在，量或关联的方法和化合物。该方法基于聚合诱导的分离来自游离的报道分子标记的识别反应物的特异结合的报道分子标记的识别反应物。所描述的方法适用于存在或可以制备合适的识别反应物的任何物质，并且不受诸如化学组成或分子大小等考虑的限制。

2. 发明授权

US4752638A Synthesis and use of polymers containing integral binding-pair members 失效
标题翻译：含有整体结合对成员的聚合物的合成和使用
公开(公告)号：US4752638A
公开(公告)日：1988-06-21
申请号：US880122
申请日：1986-06-30
申请人： Robert C. Nowinski , Allan S. Hoffman , Raymond L. Houghton , John H. Priest , Nobuo Monji
发明人： Robert C. Nowinski , Allan S. Hoffman , Raymond L. Houghton , John H. Priest , Nobuo Monji
IPC分类号： C08F299/02 , G01N33/545 , C08L89/00 , C08H1/00
CPC分类号： C08F299/022 , G01N33/545 , G01N2650/00 , Y10S436/827 , Y10S530/817
摘要： Conjugate monomers, polymers, and methods for the de novo synthesis of the polymers are provided. Conjugate organic monomers contain binding-pair members which upon polymerization become integrally associated with the resultant polymer. Specifically, antigens, antibodies, receptors, and ligands may be bound to organic monomers either directly by chemical reaction or indirectly by chemical spacer arms, and these conjugates may be polymerized or copolymerized with nonderivatized monomers to form polymers containing variable amounts of the binding-pair members. Such conjugate monomers and polymers find a wide variety of uses in binding to their binding-pair-member cognate which include selective removal of complementary binding-pair members from solution as well as in immunoassay procedures and in immunization regimes.
摘要翻译：提供了用于聚合物的从头合成的共轭单体，聚合物和方法。共轭有机单体含有结合对成员，其在聚合时与所得聚合物整体相关。具体地，抗原，抗体，受体和配体可以直接通过化学反应或通过化学间隔臂间接地结合到有机单体上，并且这些缀合物可以与非稀释的单体聚合或共聚以形成含有可变量的结合对的聚合物会员这种缀合物单体和聚合物在结合它们的结合对成员同源物中发现了各种各样的用途，其包括从溶液以及免疫测定方法和免疫方案中选择性除去互补结合对成员。

3. 发明授权

US4780409A Thermally induced phase separation immunoassay 失效
标题翻译：热诱导相分离免疫测定
公开(公告)号：US4780409A
公开(公告)日：1988-10-25
申请号：US854831
申请日：1986-04-28
申请人： Nobuo Monji , Allan S. Hoffman , John H. Priest , Raymond L. Houghton
发明人： Nobuo Monji , Allan S. Hoffman , John H. Priest , Raymond L. Houghton
IPC分类号： G01N33/543 , G01N33/531 , G01N33/537 , G01N33/545 , G01N33/569
CPC分类号： G01N33/545 , G01N33/531 , G01N33/5375 , G01N33/56927 , Y10S435/971 , Y10S435/973 , Y10S436/824 , Y10S436/827
摘要： An immunoassay in which a thermally induced phase separation is used to effect the separation of specifically bound reactants from free reactants is disclosed. A first reactant is conjugated to a temperature-sensitive polymer to form a polymer/reactant conjugate, and a second reactant is conjugated to a reporter to form a reporter/reactant conjugate. The polymer/reactant, reporter/reactant, and biological fluid samples suspected of containing the analyte are admixed in solution at a temperature other than that at which the polymer will precipitate. Specific binding is allowed to occur, thereby forming a ternary complex. The salt concentration of the adjusted solution is then adjusted to a concentration sufficient to cause the complex to precipitate from the solution, the amount of reporter activity in the precipitated complex or in the solution measured and the presence and/or concentration of the analyte therefrom determined. Alternatively, the first reactant may be conjugated to a monomer and subsequently copolymerized with additional monomers to yield a temperature-sensitive copolymer. Multiple analyses may also be performed on a single sample by choosing a variety of polymers, each polymer having a different specific binding partner conjugated thereto and a different critical solution temperature. By altering the temperature and/or the salt concentration of the solution incrementally, the reporter associated with each of the complexes precipitated with each temperature or concentration increment may be measured, and the presence and/or concentration of each of the analytes determined.
摘要翻译：公开了一种免疫测定法，其中使用热诱导相分离来实现特异性结合的反应物与游离反应物的分离。将第一反应物与温度敏感的聚合物缀合以形成聚合物/反应物缀合物，并且将第二反应物与报道分子缀合以形成报告物/反应物缀合物。怀疑含有分析物的聚合物/反应物，报告物/反应物和生物液体样品在除聚合物沉淀之外的温度下在溶液中混合。允许发生特异性结合，从而形成三元复合物。然后将调整后的溶液的盐浓度调整至足以引起络合物从溶液中沉淀出的浓度，测定沉淀的络合物或溶液中的报告物活性的量以及测定的分析物的存在和/或浓度。或者，第一反应物可以与单体缀合，随后与另外的单体共聚以产生温度敏感的共聚物。也可以通过选择各种聚合物对单个样品进行多重分析，每种聚合物具有与其缀合的不同的特异性结合对象和不同的临界溶解温度。通过逐渐改变溶液的温度和/或盐浓度，可以测量与每个温度或浓度增量沉淀的每个络合物相关的报告物，并测定每种分析物的存在和/或浓度。

4. 发明授权

US06750962B2 Optics alignment and calibration system 有权
标题翻译：光学对准和校准系统
公开(公告)号：US06750962B2
公开(公告)日：2004-06-15
申请号：US09828874
申请日：2001-04-10
申请人： Joel S. Douglas , Jeffrey N. Roe , John H. Priest , John M. Gleisner , Charles C. Raney , David A. Hasker , Ryszard Radwanski , John Ramirez
发明人： Joel S. Douglas , Jeffrey N. Roe , John H. Priest , John M. Gleisner , Charles C. Raney , David A. Hasker , Ryszard Radwanski , John Ramirez
IPC分类号： G01N110
CPC分类号： G01N21/8483
摘要： A multi-use assay system accurately docks a removable test strip supporting a reagent-analyte reaction with an optics system including an LED, photodetector, and lenses or light pipes for directing light to and from the test strip. Docking is achieved using alignment fixturing, whereby an optics block holder is relied upon to align the test strip and test pad with the various optical components. Signals from the photodetector indicative of test strip reaction progress are provided to a processor for measuring the analyte.
摘要翻译：多用分析系统将包含LED，光电检测器和透镜或光管的光学系统的支撑试剂 - 分析物反应的可移除测试条准确地对准，以将光导向和从测试条引导。使用对准夹具实现对接，由此依靠光学块保持器将测试条和测试垫与各种光学部件对准。来自光电检测器的指示测试条反应进程的信号被提供给用于测量分析物的处理器。

5. 发明授权

US5587286A Methods and kits for detection of cells in food materials 失效
标题翻译：用于检测食物中细胞的方法和试剂盒
公开(公告)号：US5587286A
公开(公告)日：1996-12-24
申请号：US3242
申请日：1993-01-11
申请人： Edward E. Pahuski , Randall L. Dimond , John H. Priest , Lisa Zandt , Kathleen K. Stebnitz , Leopoldo G. Mendoza
发明人： Edward E. Pahuski , Randall L. Dimond , John H. Priest , Lisa Zandt , Kathleen K. Stebnitz , Leopoldo G. Mendoza
IPC分类号： C12M1/34 , C07K1/36 , C12N1/02 , C12Q1/02 , C12Q1/04 , C12Q1/06 , C12Q1/10 , C12Q1/24 , C12Q1/37 , C12Q1/66 , C12Q1/68 , G01N33/48 , G01N33/50
CPC分类号： C07K1/36 , C12Q1/04 , C12Q1/10 , C12Q1/24 , G01N33/50 , C12Q2304/60 , Y10T436/25375
摘要： Processes are provided for removal of cells as cell pellets from liquid milk samples, or from cultures or extracts of other food materials or other materials of biological origin. The concentrated cells in the pellet can be analyzed by various techniques to determine the relative cell count, such as by lysing of the cells followed by measurement of ATP. Nucleic amplification, as by the polymerase chain reaction method, can be carried out using the cellular pellet directly, without need for isolation of nucleic acid from the cells. After the amplification, an assay can be carried out for amplified nucleic acid segment indicative of the presence of cells of interest in the sample. The invention thus provides methods for obtaining cellular components from samples of milk, and cultures or extracts of other materials, including food materials, and for determining relative contamination of milk and such other materials by microorganisms. The invention also provides kits for carrying out its various methods.
摘要翻译：提供了从液体乳样品或其他食品材料或其他生物来源的材料的培养物或提取物中除去作为细胞沉淀物的细胞的方法。可以通过各种技术分析沉淀中的浓缩细胞以确定相对细胞计数，例如通过裂解细胞，随后测量ATP。如通过聚合酶链反应方法的核扩增可以直接使用细胞沉淀进行，而不需要从细胞中分离核酸。在扩增后，可以对指示样品中感兴趣的细胞的存在的扩增的核酸片段进行测定。因此，本发明提供了从牛奶样品和其他材料（包括食品材料）的培养物或提取物中获得细胞成分的方法，以及用于通过微生物确定牛奶和其它物质的相对污染。本发明还提供用于实施其各种方法的试剂盒。

6. 发明授权

US07018848B2 Electrochemical test device and related methods 失效
公开(公告)号：US07018848B2
公开(公告)日：2006-03-28
申请号：US10224875
申请日：2002-08-21
申请人： Joel S. Douglas , Jeffrey N. Roe , John H. Priest
发明人： Joel S. Douglas , Jeffrey N. Roe , John H. Priest
IPC分类号： G01N33/551
CPC分类号： C12Q1/001 , C12Q1/004 , C12Q1/006 , Y10S435/817 , Y10S436/805 , Y10S436/806
摘要： An electrochemical test device is provided for determining the presence or concentration of an analyte in an aqueous fluid sample. The electrochemical test device includes a working electrode and a counter electrode made of an amorphous semiconductor material. The working electrode is overlaid with a reagent capable of reacting with an analyte to produce a measurable change in potential which can be correlated to the concentration of the analyte in the fluid sample. The test device optionally contains a reference electrode made of an amorphous semiconductor material having a reference material on the reference electrode. The test device electrodes can be constructed on a flexible film substrate, such as a polymeric film or a metal foil coated with a non-conductive coating.

7. 发明授权

US06780651B2 Device for determination of an analyte in a body fluid 失效
标题翻译：用于测定体液中分析物的装置
公开(公告)号：US06780651B2
公开(公告)日：2004-08-24
申请号：US09875297
申请日：2001-06-07
申请人： Joel S. Douglas , Ryszard Radwanski , Brent G. Duchon , John H. Priest , David A. Hasker , John M. Gleisner
发明人： Joel S. Douglas , Ryszard Radwanski , Brent G. Duchon , John H. Priest , David A. Hasker , John M. Gleisner
IPC分类号： G01N33543
CPC分类号： G01N33/521 , B01L3/5023 , B01L2200/026 , B01L2200/0605 , B01L2300/0825 , B01L2300/0887 , B01L2400/0406 , G01N33/525 , Y10S435/805 , Y10S435/81 , Y10S435/97 , Y10S436/805 , Y10S436/81
摘要： A method of making strip for testing for the presence of an analyte generally comprises providing a support member which includes a spreading layer and a reagent layer, and a capillary in communication with the support layer and spreading layer for transporting a sample of body fluid thereto.
摘要翻译：制造用于测试分析物存在的条带的方法通常包括提供包括扩散层和试剂层的支撑构件，以及与支撑层连通的毛细管和用于将体液样品输送到其的扩散层。

8. 发明申请

US20110275916A1 Control solution packets and methods for calibrating bodily fluid sampling devices 审中-公开
标题翻译：控制溶液包和校准体液采样装置的方法
公开(公告)号：US20110275916A1
公开(公告)日：2011-11-10
申请号：US11153854
申请日：2005-06-15
申请人： Jeffrey N. Roe , Michael E. Hilgers , Jay Vinson , John H. Priest , Steven P. Mecca , Thomas C. Rademacher
发明人： Jeffrey N. Roe , Michael E. Hilgers , Jay Vinson , John H. Priest , Steven P. Mecca , Thomas C. Rademacher
IPC分类号： A61B5/145
CPC分类号： A61B5/157 , A61B5/14514 , A61B5/14532 , A61B5/150022 , A61B5/150175 , A61B5/150358 , A61B5/150412 , A61B5/150503 , A61B5/15144 , A61B5/1519 , A61B5/15194 , A61B2562/0295 , Y10T436/10 , Y10T436/102499 , Y10T436/103332 , Y10T436/104165 , Y10T436/104998 , Y10T436/105831 , Y10T436/106664 , Y10T436/109163
摘要： A control solution packet for calibrating a bodily fluid sampling device includes a container, a control solution pressurized within the container, and a membrane for covering and sealing the container. The control solution can be pressurized before or during calibration so as to ensure the appropriate amount of control solution is delivered to the bodily fluid sampling device. The control solution is manufactured to have a viscosity that controls delivery of the control solution to the device. The membrane is permeable by a piercing device of the bodily fluid sampling device and seals around the piercing device during calibration. In another aspect, the container is in the form of a capsule or dosing attachment that contains the control solution along with a sponge-like material.
摘要翻译：用于校准体液采样装置的控制解决方案包包括容器，在容器内加压的对照溶液以及用于覆盖和密封容器的膜。在校准之前或期间，可以对对照溶液进行加压，以确保将适当量的对照溶液输送到体液采样装置。控制解决方案被制造成具有控制控制溶液输送到装置的粘度。该膜可通过体液采样装置的穿刺装置渗透，并在校准过程中围绕穿刺装置密封。在另一方面，容器是胶囊或剂量附件的形式，其包含对照溶液以及海绵状材料。

9. 发明授权

US07115362B2 Electrochemical test device and related methods 失效
公开(公告)号：US07115362B2
公开(公告)日：2006-10-03
申请号：US11287522
申请日：2005-11-23
申请人： Joel S. Douglas , Jeffrey N. Roe , John H. Priest
发明人： Joel S. Douglas , Jeffrey N. Roe , John H. Priest
IPC分类号： C12Q1/00
CPC分类号： C12Q1/001 , C12Q1/004 , C12Q1/006 , Y10S435/817 , Y10S436/805 , Y10S436/806
摘要： An electrochemical test device is provided for determining the presence or concentration of an analyte in an aqueous fluid sample. The electrochemical test device includes a working electrode and a counter electrode made of an amorphous semiconductor material. The working electrode is overlaid with a reagent capable of reacting with an analyte to produce a measurable change in potential which can be correlated to the concentration of the analyte in the fluid sample. The test device optionally contains a reference electrode made of an amorphous semiconductor material having a reference material on the reference electrode. The test device electrodes can be constructed on a flexible film substrate, such as a polymeric film or a metal foil coated with a non-conductive coating.

10. 发明授权

US6001239A Membrane based electrochemical test device and related methods 失效
公开(公告)号：US6001239A
公开(公告)日：1999-12-14
申请号：US164405
申请日：1998-09-30
申请人： Joel S. Douglas , Jeffrey N. Roe , John H. Priest , David A. Hasker
发明人： Joel S. Douglas , Jeffrey N. Roe , John H. Priest , David A. Hasker
IPC分类号： C12Q1/00 , C12Q1/26 , G01N33/487 , G01N33/49 , G01N33/543 , G01N27/26
CPC分类号： C12Q1/001 , C12Q1/26 , G01N27/3272 , G01N33/54386
摘要： An electrochemical test device is provided for determining the presence or concentration of an analyte in an aqueous fluid sample. The electrochemical test device includes a working electrode and a counter electrode made of an amorphous semiconductor material. The working electrode is overlaid with a reagent capable of reacting with an analyte to produce a measurable change in potential which can be correlated to the concentration of the analyte in the fluid sample. The test device optionally contains a reference electrode made of an amorphous semiconductor material having a reference material on the reference electrode.

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