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1. 发明授权

US08692187B2 Electron transfer dissociation for biopolymer sequence analysis 有权
标题翻译：用于生物聚合物序列分析的电子转移解离
公开(公告)号：US08692187B2
公开(公告)日：2014-04-08
申请号：US13422812
申请日：2012-03-16
申请人： Donald F. Hunt , Joshua J. Coon , John Edward Philip Syka , Jarod A. Marto
发明人： Donald F. Hunt , Joshua J. Coon , John Edward Philip Syka , Jarod A. Marto
IPC分类号： H01J49/00
CPC分类号： H01J49/423 , G01N33/6848 , H01J49/0072 , Y10T436/212 , Y10T436/24
摘要： The present invention relates to a new method for fragmenting ions in a mass spectrometer through the use of electron transfer dissociation, and for performing sequence analysis of peptides and proteins by mass spectrometry. In the case of peptides, the invention promotes fragmentation along the peptide backbone and makes it possible to deduce the amino acid sequence of the sample, including modified amino acid residues, through the use of an RF field device.
摘要翻译：本发明涉及通过使用电子转移离解在质谱仪中分离离子的新方法，以及通过质谱法进行肽和蛋白质的序列分析。在肽的情况下，本发明促进沿肽主链的断裂，并且可以通过使用RF场设备来推断样品的氨基酸序列，包括修饰的氨基酸残基。

2. 发明授权

US07749769B2 Simultaneous sequence analysis of amino- and carboxy-termini 有权
标题翻译：氨基和羧基末端的同时序列分析
公开(公告)号：US07749769B2
公开(公告)日：2010-07-06
申请号：US11664661
申请日：2005-10-07
申请人： Donald F Hunt , Joshua J. Coon , John Edward Philip Syka
发明人： Donald F Hunt , Joshua J. Coon , John Edward Philip Syka
IPC分类号： G01N24/00 , H01J49/00
CPC分类号： G01N33/6848 , G01N33/6818 , Y10T436/24 , Y10T436/25875
摘要： The present invention relates to a new method for identifying polypeptides by deducing the amino acid sequence of the carboxy and amino termini by a mass spectrometer analysis. The method comprises the steps of dissociating highly charged peptide precursor ions (e.g., z>4) using electron transfer dissociation inducing anions followed by removal of those reagents and introduction of a second, proton transfer inducing anion type. The second PTR reaction duration is adjusted to convert the ETD products to primarily the +1 charge-state to reduce the highly charged c and z-type fragments, producing an m/z spectrum containing a series of c and z-type fragment ions that are easily interpreted to reveal the sequence of the amino and carboxy terminus, respectively.
摘要翻译：本发明涉及通过质谱分析推断羧基和氨基末端的氨基酸序列来鉴定多肽的新方法。该方法包括以下步骤：使用电子转移离解诱导阴离子，然后除去那些试剂和引入第二种质子转移诱导阴离子型，使高度带电的肽前体离子（例如z> 4）解离。调整第二个PTR反应持续时间以将ETD产物转化为主要的+1电荷状态以减少高电荷的c和z型片段，产生含有一系列c和z型片段离子的m / z光谱，容易解释为分别显示氨基和羧基末端的序列。

3. 发明授权

US07534622B2 Electron transfer dissociation for biopolymer sequence mass spectrometric analysis 有权
标题翻译：电子转移解离生物聚合物序列质谱分析
公开(公告)号：US07534622B2
公开(公告)日：2009-05-19
申请号：US11079147
申请日：2005-03-14
申请人： Donald F. Hunt , Joshua J. Coon , John Edward Philip Syka , Jarrod A. Marto
发明人： Donald F. Hunt , Joshua J. Coon , John Edward Philip Syka , Jarrod A. Marto
IPC分类号： G01N24/00
CPC分类号： H01J49/423 , G01N33/6848 , H01J49/0072 , Y10T436/212 , Y10T436/24
摘要： The present invention relates to a new method for fragmenting ions in a mass spectrometer through the use of electron transfer dissociation, and for performing sequence analysis of peptides and proteins by mass spectrometry. In the case of peptides, the invention promotes fragmentation along the peptide backbone and makes it possible to deduce the amino acid sequence of the sample, including modified amino acid residues, through the use of an RF field device.
摘要翻译：本发明涉及通过使用电子转移离解在质谱仪中分离离子的新方法，以及通过质谱法进行肽和蛋白质的序列分析。在肽的情况下，本发明促进沿肽主链的断裂，并且可以通过使用RF场设备来推断样品的氨基酸序列，包括修饰的氨基酸残基。

4. 发明授权

US11538675B2 Rapid identification and sequence analysis of intact proteins in complex mixtures 有权
公开(公告)号：US11538675B2
公开(公告)日：2022-12-27
申请号：US16307615
申请日：2017-06-05
申请人： Donald F. Hunt , Ann M. English , Scott A. Ugrin , Dina L. Bai , Jeffrey Shabanowitz , John Edward Philip Syka
发明人： Donald F. Hunt , Ann M. English , Scott A. Ugrin , Dina L. Bai , Jeffrey Shabanowitz , John Edward Philip Syka
IPC分类号： H01J49/00 , G01N33/68 , H01J49/04 , H01J49/42
摘要： The present disclosure relates to novel and improved methods of analyzing proteins, peptides and polypeptides by mass spectrometry using ion-ion reactions. More specifically the disclosure relates to improved methods for implementing the m/z selective arresting of ion-ion reactions within the ion-ion reaction cell of a mass spectrometer system during a period where ion-ion reactions are performed.

5. 发明授权

US06392225B1 Method and apparatus for transferring ions from an atmospheric pressure ion source into an ion trap mass spectrometer 有权
标题翻译：将离子从大气压离子源转移到离子阱质谱仪中的方法和装置
公开(公告)号：US06392225B1
公开(公告)日：2002-05-21
申请号：US09160639
申请日：1998-09-24
申请人： Jae C. Schwartz , John Edward Philip Syka
发明人： Jae C. Schwartz , John Edward Philip Syka
IPC分类号： H01J4942
CPC分类号： H01J49/063 , H01J49/061
摘要： An ion transfer assembly for transferring ions from an atmospheric pressure ion source into an ion trap mass spectrometer with reduced random noise during analysis of the transferred ions. A method of reducing noise due to charged particles, undesolvated charged droplets, or ions in an ion trap mass spectrometer connected to an atmospheric pressure ionization source.
摘要翻译：用于将离子从大气压离子源转移到离子阱质谱仪中的离子转移组件，其在转移离子的分析期间具有降低的随机噪声。在与大气压电离源连接的离子阱质谱仪中降低由于带电粒子，未溶化的带电液滴或离子引起的噪声的方法。

6. 发明授权

US07157698B2 Obtaining tandem mass spectrometry data for multiple parent ions in an ion population 有权
标题翻译：在离子群体中获得多个亲本离子的串联质谱数据
公开(公告)号：US07157698B2
公开(公告)日：2007-01-02
申请号：US10804692
申请日：2004-03-19
申请人： Alexander Alekseevich Makarov , John Edward Philip Syka
发明人： Alexander Alekseevich Makarov , John Edward Philip Syka
IPC分类号： B01D59/44 , H01J49/00 , H01J49/40
CPC分类号： H01J49/063 , H01J49/004 , H01J49/423
摘要： This invention relates to tandem mass spectrometry and, in particular, to tandem mass spectrometry using a linear ion trap and a time of flight detector to collect mass spectra to form a MS/MS experiment. The accepted standard is to store and mass analyze precursor ions in the ion trap before ejecting the ions axially to a collision cell for fragmentation before mass analysis of the fragments in the time of flight detector. This invention makes use of orthogonal ejection of ions with a narrow range of m/z values to produce a ribbon beam of ions that are injected into the collision cell. The shape of this beam and the high energy of the ions are accommodated by using a planar design of collision cell. Ions are retained in the ion trap during ejection so that successive narrow ranges may be stepped through consecutively to cover all precursor ions of interest.
摘要翻译：本发明涉及串联质谱法，特别涉及使用线性离子阱和飞行时间检测器的串联质谱法来收集质谱以形成MS / MS实验。所接受的标准是在离子阱中存储和质量分析前体离子，然后在飞行时间内对碎片进行质量分析之前，将离子轴向排列到碰撞池进行碎裂。本发明利用具有窄范围m / z值的离子的正交喷射来产生注入到碰撞池中的带状离子束束。通过使用碰撞池的平面设计来容纳该束的形状和离子的高能量。在喷射期间离子被保留在离子阱中，使得连续的窄范围可以连续地步进以覆盖所有感兴趣的前体离子。

7. 发明授权

US07507953B2 Obtaining tandem mass spectrometry data for multiple parent ions in an ion population 有权
标题翻译：在离子群体中获得多个亲本离子的串联质谱数据
公开(公告)号：US07507953B2
公开(公告)日：2009-03-24
申请号：US12018070
申请日：2008-01-22
申请人： Alexander Alekseevich Makarov , John Edward Philip Syka
发明人： Alexander Alekseevich Makarov , John Edward Philip Syka
IPC分类号： H01J49/40 , H01J49/04 , H01J49/42 , H01L49/00
CPC分类号： H01J49/063 , H01J49/004 , H01J49/423
摘要： This invention relates to tandem mass spectrometry and, in particular, to tandem mass spectrometry using a linear ion trap and a time of flight detector to collect mass spectra to form a MS/MS experiment. The accepted standard is to store and mass analyze precursor ions in the ion trap before ejecting the ions axially to a collision cell for fragmentation before mass analysis of the fragments in the time of flight detector. This invention makes use of orthogonal ejection of ions with a narrow range of m/z values to produce a ribbon beam of ions that are injected into the collision cell. The shape of this beam and the high energy of the ions are accommodated by using a planar design of collision cell. Ions are retained in the ion trap during ejection so that successive narrow ranges may be stepped through consecutively to cover all precursor ions of interest.
摘要翻译：本发明涉及串联质谱法，特别涉及使用线性离子阱和飞行时间检测器的串联质谱法来收集质谱以形成MS / MS实验。所接受的标准是在离子阱中存储和质量分析前体离子，然后在飞行时间内对碎片进行质量分析之前，将离子轴向排列到碰撞池进行碎裂。本发明利用具有窄范围m / z值的离子的正交喷射来产生注入到碰撞池中的带状离子束束。通过使用碰撞池的平面设计来容纳该束的形状和离子的高能量。在喷射期间离子被保留在离子阱中，使得连续的窄范围可以连续地步进以覆盖所有感兴趣的前体离子。

8. 发明授权

US07342224B2 Obtaining tandem mass spectrometry data for multiple parent ions in an ion population 有权
标题翻译：在离子群体中获得多个亲本离子的串联质谱数据
公开(公告)号：US07342224B2
公开(公告)日：2008-03-11
申请号：US11494405
申请日：2006-07-26
申请人： Alexander Alekseevich Makarov , John Edward Philip Syka
发明人： Alexander Alekseevich Makarov , John Edward Philip Syka
IPC分类号： H01L49/10 , H01L49/00
CPC分类号： H01J49/063 , H01J49/004 , H01J49/423
摘要： This invention relates to tandem mass spectrometry and, in particular, to tandem mass spectrometry using a linear ion trap and a time of flight detector to collect mass spectra to form a MS/MS experiment. The accepted standard is to store and mass analyze precursor ions in the ion trap before ejecting the ions axially to a collision cell for fragmentation before mass analysis of the fragments in the time of flight detector. This invention makes use of orthogonal ejection of ions with a narrow range of m/z values to produce a ribbon beam of ions that are injected into the collision cell. The shape of this beam and the high energy of the ions are accommodated by using a planar design of collision cell. Ions are retained in the ion trap during ejection so that successive narrow ranges may be stepped through consecutively to cover all precursor ions of interest.
摘要翻译：本发明涉及串联质谱法，特别涉及使用线性离子阱和飞行时间检测器的串联质谱法来收集质谱以形成MS / MS实验。所接受的标准是在离子阱中存储和质量分析前体离子，然后在飞行时间内对碎片进行质量分析之前，将离子轴向排列到碰撞池进行碎裂。本发明利用具有窄范围m / z值的离子的正交喷射来产生注入到碰撞池中的带状离子束束。通过使用碰撞池的平面设计来容纳该束的形状和离子的高能量。在喷射期间离子被保留在离子阱中，使得连续的窄范围可以连续地步进以覆盖所有感兴趣的前体离子。

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