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1. 发明授权

US11538675B2 Rapid identification and sequence analysis of intact proteins in complex mixtures 有权
公开(公告)号：US11538675B2
公开(公告)日：2022-12-27
申请号：US16307615
申请日：2017-06-05
申请人： Donald F. Hunt , Ann M. English , Scott A. Ugrin , Dina L. Bai , Jeffrey Shabanowitz , John Edward Philip Syka
发明人： Donald F. Hunt , Ann M. English , Scott A. Ugrin , Dina L. Bai , Jeffrey Shabanowitz , John Edward Philip Syka
IPC分类号： H01J49/00 , G01N33/68 , H01J49/04 , H01J49/42
摘要： The present disclosure relates to novel and improved methods of analyzing proteins, peptides and polypeptides by mass spectrometry using ion-ion reactions. More specifically the disclosure relates to improved methods for implementing the m/z selective arresting of ion-ion reactions within the ion-ion reaction cell of a mass spectrometer system during a period where ion-ion reactions are performed.

2. 发明申请

US20130259883A1 CLASS I MHC PHOSPHOPEPTIDES FOR CANCER IMMUNOTHERAPY AND DIAGNOSIS 审中-公开
标题翻译：第一类MHC磷酸化酶对于癌症的免疫和诊断
公开(公告)号：US20130259883A1
公开(公告)日：2013-10-03
申请号：US13699563
申请日：2011-05-24
申请人： Donald F. Hunt , Jeffrey Shabanowitz , Jennifer Cottine , Ann M. English , Andrew Norris , Victor H. Engelhard , Mark Cobbold , Kara L. Cummings , Angela Zarling , Rebecca C. Obeng , Jie Qian
发明人： Donald F. Hunt , Jeffrey Shabanowitz , Jennifer Cottine , Ann M. English , Andrew Norris , Victor H. Engelhard , Mark Cobbold , Kara L. Cummings , Angela Zarling , Rebecca C. Obeng , Jie Qian
IPC分类号： C07K7/06 , C07K16/18 , G01N33/68 , C07K7/08 , A61K39/00
CPC分类号： C07K7/06 , A61K38/00 , A61K39/0011 , C07K7/08 , C07K14/70539 , C07K16/18 , G01N33/5743 , G01N33/57492 , G01N33/6893 , G01N2333/70539 , G01N2440/14
摘要： A set of phosphorylated peptides are presented by HLA A*0101, A*0201, A*0301, B*4402, B*2705, B*1402, and B*0702 on the surface of melanoma cells. They have the potential to (a) stimulate an immune response to the cancer, (b) to function as immunotherapeutics in adoptive T-cell therapy or as a vaccine, (c) to facilitate antibody recognition of the tumor boundaries in surgical pathology samples, and (d) act as biomarkers for early detection of the disease. Phosphorylated peptides are also presented for other cancers.
摘要翻译：一组磷酸化肽由黑素瘤细胞表面上的HLA A * 0101，A * 0201，A * 0301，B * 4402，B * 2705，B * 1402和B * 0702呈现。它们有可能（a）刺激对癌症的免疫应答，（b）在过继性T细胞治疗或疫苗中起到免疫治疗的作用，（c）促进外科病理样本中肿瘤边界的抗体识别，和（d）作为早期检测疾病的生物标志物。磷酸化肽也可用于其他癌症。

3. 发明授权

US08692187B2 Electron transfer dissociation for biopolymer sequence analysis 有权
标题翻译：用于生物聚合物序列分析的电子转移解离
公开(公告)号：US08692187B2
公开(公告)日：2014-04-08
申请号：US13422812
申请日：2012-03-16
申请人： Donald F. Hunt , Joshua J. Coon , John Edward Philip Syka , Jarod A. Marto
发明人： Donald F. Hunt , Joshua J. Coon , John Edward Philip Syka , Jarod A. Marto
IPC分类号： H01J49/00
CPC分类号： H01J49/423 , G01N33/6848 , H01J49/0072 , Y10T436/212 , Y10T436/24
摘要： The present invention relates to a new method for fragmenting ions in a mass spectrometer through the use of electron transfer dissociation, and for performing sequence analysis of peptides and proteins by mass spectrometry. In the case of peptides, the invention promotes fragmentation along the peptide backbone and makes it possible to deduce the amino acid sequence of the sample, including modified amino acid residues, through the use of an RF field device.
摘要翻译：本发明涉及通过使用电子转移离解在质谱仪中分离离子的新方法，以及通过质谱法进行肽和蛋白质的序列分析。在肽的情况下，本发明促进沿肽主链的断裂，并且可以通过使用RF场设备来推断样品的氨基酸序列，包括修饰的氨基酸残基。

4. 发明授权

US07534622B2 Electron transfer dissociation for biopolymer sequence mass spectrometric analysis 有权
标题翻译：电子转移解离生物聚合物序列质谱分析
公开(公告)号：US07534622B2
公开(公告)日：2009-05-19
申请号：US11079147
申请日：2005-03-14
申请人： Donald F. Hunt , Joshua J. Coon , John Edward Philip Syka , Jarrod A. Marto
发明人： Donald F. Hunt , Joshua J. Coon , John Edward Philip Syka , Jarrod A. Marto
IPC分类号： G01N24/00
CPC分类号： H01J49/423 , G01N33/6848 , H01J49/0072 , Y10T436/212 , Y10T436/24
摘要： The present invention relates to a new method for fragmenting ions in a mass spectrometer through the use of electron transfer dissociation, and for performing sequence analysis of peptides and proteins by mass spectrometry. In the case of peptides, the invention promotes fragmentation along the peptide backbone and makes it possible to deduce the amino acid sequence of the sample, including modified amino acid residues, through the use of an RF field device.
摘要翻译：本发明涉及通过使用电子转移离解在质谱仪中分离离子的新方法，以及通过质谱法进行肽和蛋白质的序列分析。在肽的情况下，本发明促进沿肽主链的断裂，并且可以通过使用RF场设备来推断样品的氨基酸序列，包括修饰的氨基酸残基。

5. 发明授权

US4175234A Apparatus for producing ions of thermally labile or nonvolatile solids 失效
标题翻译：用于产生热不稳定或非挥发性固体的离子的装置
公开(公告)号：US4175234A
公开(公告)日：1979-11-20
申请号：US916151
申请日：1978-06-16
申请人： Donald F. Hunt , Jeffrey Shabanowitz
发明人： Donald F. Hunt , Jeffrey Shabanowitz
IPC分类号： H01J49/14 , H01J49/16 , H01J27/00
CPC分类号： H01J49/145 , H01J49/16
摘要： An apparatus for ionizing and vaporizing non-volatile or thermally labile molecules of a sample for introduction into a mass spectrometer analyzer which comprises a housing containing an ionization chamber; an ion exit port for the introduction of gaseous ions from said ionization chamber into a mass spectrometer analyzer on the portion of the housing attached to said mass spectrometer analyzer; inlet means for introducing a gaseous reactant into said ionization chamber within said housing; inlet means for admitting high energy radiation into said ionization chamber within said housing to at least partially ionize the molecules of said gaseous reactant; aperture means open into said ionization chamber of said housing for admitting a conductive emitter characterized by having a conductive element of a highly irregular surface upon which is deposited said sample for analysis into said mass spectrometer analyzer; and a vacuum exit aperture in said housing by which the pressure within said ionization chamber can be reduced.
摘要翻译：一种用于离子化和蒸发样品的非挥发性或热不稳定分子的装置，用于引入质谱仪分析仪，其包括含有电离室的壳体; 离子出口，用于将气态离子从所述电离室引入质谱仪分析仪，所述质谱仪分析仪附着在所述质谱仪分析仪上; 用于将气体反应物引入所述壳体内的所述电离室的入口装置; 入口装置，用于将高能量辐射进入所述壳体内的所述电离室，以使所述气态反应物的分子至少部分电离; 开口意味着通向所述壳体的所述电离室，用于允许导电发射器，其特征在于具有高度不规则表面的导电元件，在其上沉积所述样品用于分析所述质谱仪分析仪; 以及所述壳体中的真空出口孔，通过该真空出口孔可以减小所述电离室内的压力。

6. 发明授权

US4156814A Apparatus for producing ions of thermally labile or nonvolatile solids 失效
公开(公告)号：US4156814A
公开(公告)日：1979-05-29
申请号：US822216
申请日：1977-08-05
申请人： Donald F. Hunt , Jeffrey Shabanowitz
发明人： Donald F. Hunt , Jeffrey Shabanowitz
IPC分类号： H01J49/04 , H01J49/14
CPC分类号： H01J49/0409 , H01J49/142
摘要： An apparatus for ionizing and vaporizing non-volatile or thermally labile molecules of a sample for introduction into a mass spectrometer analyzer which comprises a housing containing an ionization chamber; an ion exit port for the introduction of gaseous ions from said ionization chamber into a mass spectrometer analyzer on the portion of the housing attached to said mass spectrometer analyzer; inlet means for introducing a gaseous reactant into said ionization chamber within said housing; inlet means for admitting high energy radiation into said ionization chamber within said housing to at least partially ionize the molecules of said gaseous reactant; aperture means open into said ionization chamber of said housing for admitting a conductive emitter characterized by having a conductive element of a highly irregular surface upon which is deposited said sample for analysis into said mass spectrometer analyzer; and a vacuum exit aperture in said housing by which the pressure within said ionization chamber can be reduced.

7. 发明授权

US08119984B2 Method and apparatus for generation of reagent ions in a mass spectrometer 有权
标题翻译：用于在质谱仪中产生试剂离子的方法和装置
公开(公告)号：US08119984B2
公开(公告)日：2012-02-21
申请号：US12473570
申请日：2009-05-28
申请人： Jeffrey Shabanowitz , Philip D. Compton , Lee Earley , George C. Stafford, Jr. , Donald F. Hunt , Christopher Mullen
发明人： Jeffrey Shabanowitz , Philip D. Compton , Lee Earley , George C. Stafford, Jr. , Donald F. Hunt , Christopher Mullen
IPC分类号： H01J49/10
CPC分类号： H01J49/12 , H01J49/0072
摘要： A front-end reagent ion source for a mass spectrometer is disclosed. Reagent vapor is supplied to a reagent ionization volume located within a chamber of the mass spectrometer and maintained at a low vacuum pressure. Reagent ions are formed by interaction of the reagent vapor molecules with an electrical discharge (e.g., a glow discharge) within the ionization volume, and pass into the chamber of the mass spectrometer. At least one ion optical element located along the analyte ion path transports the reagent ions to successive chambers of the mass spectrometer. The reagent ions may be combined with the analyte ions to perform ion-ion studies such as electron transfer dissociation (ETD).
摘要翻译：公开了用于质谱仪的前端试剂离子源。将试剂蒸气供给到位于质谱仪室内的试剂电离体并保持在低真空压力下。试剂离子通过试剂蒸气分子与离子化体积内的放电（例如，辉光放电）的相互作用而形成，并进入质谱仪的室。沿着分析物离子路径定位的至少一个离子光学元件将试剂离子输送到质谱仪的连续室。试剂离子可与分析物离子组合以进行离子离子研究，例如电子转移离解（ETD）。

8. 发明申请

US20090294649A1 Method and Apparatus for Generation of Reagent Ions in a Mass Spectrometer 有权
标题翻译：用于在质谱仪中产生试剂离子的方法和装置
公开(公告)号：US20090294649A1
公开(公告)日：2009-12-03
申请号：US12473570
申请日：2009-05-28
申请人： Jeffrey Shabanowitz , Philip D. Compton , Lee Earley , George C. Stafford, JR. , Donald F. Hunt , Christopher Mullen
发明人： Jeffrey Shabanowitz , Philip D. Compton , Lee Earley , George C. Stafford, JR. , Donald F. Hunt , Christopher Mullen
IPC分类号： B01D59/44 , H01J49/00 , H01J27/00
CPC分类号： H01J49/12 , H01J49/0072
摘要： A front-end reagent ion source for a mass spectrometer is disclosed. Reagent vapor is supplied to a reagent ionization volume located within a chamber of the mass spectrometer and maintained at a low vacuum pressure. Reagent ions are formed by interaction of the reagent vapor molecules with an electrical discharge (e.g., a glow discharge) within the ionization volume, and pass into the chamber of the mass spectrometer. At least one ion optical element located along the analyte ion path transports the reagent ions to successive chambers of the mass spectrometer. The reagent ions may be combined with the analyte ions to perform ion-ion studies such as electron transfer dissociation (ETD).
摘要翻译：公开了用于质谱仪的前端试剂离子源。将试剂蒸气供给到位于质谱仪室内的试剂电离体并保持在低真空压力下。试剂离子通过试剂蒸气分子与离子化体积内的放电（例如，辉光放电）的相互作用而形成，并进入质谱仪的室。沿着分析物离子路径定位的至少一个离子光学元件将试剂离子输送到质谱仪的连续室。试剂离子可与分析物离子组合以进行离子离子研究，例如电子转移离解（ETD）。

9. 发明授权

US07019112B1 Peptides recognized by melanoma-specific A1-, A2- and A3-restricted cytoxic lymphocytes, and uses therefor 失效
标题翻译：由黑素瘤特异性A1-，A2-和A3-限制性细胞毒性淋巴细胞识别的肽及其用途
公开(公告)号：US07019112B1
公开(公告)日：2006-03-28
申请号：US08816454
申请日：1997-03-12
申请人： Craig L. Slingluff , Donald F. Hunt , Jeffrey Shabanowitz , Andrea L. Cox , Victor H. Engelhard , David Kittlesen , Jonathan Skipper , Ronald C. Hendrikson
发明人： Craig L. Slingluff , Donald F. Hunt , Jeffrey Shabanowitz , Andrea L. Cox , Victor H. Engelhard , David Kittlesen , Jonathan Skipper , Ronald C. Hendrikson
IPC分类号： C07K7/06
CPC分类号： C07K7/06 , C07K7/08
摘要： Melanoma-specific, A1- and A3-restricted CTL epitopes have been identified in tyrosinase and pMel-17, respectively, and may be used in the design of vaccines.
摘要翻译：已经在酪氨酸酶和pMel-17中鉴定了黑素瘤特异性，A1-和A3限制性CTL表位，并且可以用于疫苗的设计。

10. 发明授权

US09279011B2 Phosphopeptides as melanoma vaccines 有权
标题翻译：磷酸肽作为黑素瘤疫苗
公开(公告)号：US09279011B2
公开(公告)日：2016-03-08
申请号：US13318821
申请日：2010-05-04
申请人： Suzanne L. Topalian , Florence A Depontieu , Donald F. Hunt , Jeffrey Shabanowitz , Jie Qian , Victor H. Engelhard , Angela Lee Zarling
发明人： Suzanne L. Topalian , Florence A Depontieu , Donald F. Hunt , Jeffrey Shabanowitz , Jie Qian , Victor H. Engelhard , Angela Lee Zarling
IPC分类号： A61K39/00 , A61K47/00 , C07K14/47 , G01N33/574 , C12Q1/68 , A61K39/39
CPC分类号： C07K14/4748 , A61K39/0011 , A61K39/39 , A61K2039/5154 , A61K2039/5158 , G01N33/5743
摘要： We characterized a total of 175 HLA-DR-associated phosphopeptides using sequential affinity isolation, biochemical enrichment, mass spectrometric sequencing and comparative analysis. Many were derived from source proteins which may have roles in cancer development, growth and metastasis. Most were expressed exclusively by either melanomas or transformed B cells, suggesting the potential to define cell type-specific phosphatome “fingerprints”. We generated HLA-DRβ1*0101-restricted CD4+ T cells specific for a phospho-MART-1 peptide identified in two melanoma cell lines. These T cells showed specificity for phosphopeptide-pulsed antigen presenting cells as well as for intact melanoma cells. MHC II-restricted phosphopeptides recognizable by human CD4+ T cells are potential targets for cancer immunotherapy.
摘要翻译：我们使用顺序亲和分离，生物化学富集，质谱测序和比较分析鉴定了总共175个HLA-DR相关磷酸肽。许多来源于可能在癌症发展，生长和转移中起作用的源蛋白质。大多数仅由黑素瘤或转化的B细胞表达，表明定义细胞类型特异性磷酸酯“指纹”的潜力。我们产生HLA-DR和bgr。1 * 0101限制性CD4 + T细胞，特异于在两个黑素瘤细胞系中鉴定的磷酸化MART-1肽。这些T细胞对磷酸肽脉冲的抗原呈递细胞以及完整的黑素瘤细胞显示出特异性。人CD4 + T细胞可识别的MHC II限制性磷酸肽是癌症免疫治疗的潜在靶标。

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