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1. 发明授权

US5455344A Agarose compositions for nucleic acid sequencing 失效
标题翻译：用于核酸测序的琼脂糖组合物
公开(公告)号：US5455344A
公开(公告)日：1995-10-03
申请号：US116662
申请日：1993-09-03
申请人： David L. Harper , Jonathan H. Morgan , Samuel Nochumson , Mikhail V. Ostrovsky , Donald W. Renn , William C. Snow
发明人： David L. Harper , Jonathan H. Morgan , Samuel Nochumson , Mikhail V. Ostrovsky , Donald W. Renn , William C. Snow
IPC分类号： C08L5/00 , G01N27/447 , C08B37/00 , C08B37/12 , C12N15/10
CPC分类号： G01N27/44747 , C08L5/00
摘要： A nucleic acid sequencing gel comprising 2 to 10% of a gelling polysaccharide, preferably agarose; a denaturing agent, a non-gelling additive, preferably glycerol, and electrophoretic buffers. This composition is capable of resolving nucleic acid fragments having at least 50 bases and which differ from each other by at least one base.
摘要翻译：包含2至10％胶凝多糖，优选琼脂糖的核酸测序凝胶; 变性剂，非胶凝添加剂，优选甘油和电泳缓冲液。该组合物能够分解具有至少50个碱基并且彼此不同的至少一个碱基的核酸片段。

2. 发明授权

US5143646A Polysaccharide resolving gels and gel systems for stacking electrophoresis 失效
标题翻译：多糖分解凝胶和凝胶体系用于层叠电泳
公开(公告)号：US5143646A
公开(公告)日：1992-09-01
申请号：US691041
申请日：1991-06-20
申请人： Samuel Nochumson , Foner P. Curtis , Jonathan H. Morgan , Francis H. Kirkpatrick
发明人： Samuel Nochumson , Foner P. Curtis , Jonathan H. Morgan , Francis H. Kirkpatrick
IPC分类号： G01N27/447 , B01D57/02 , B01J13/00 , B01J13/02 , B01J19/08 , C08B37/12 , C08L5/12 , C25D13/06
CPC分类号： G01N27/44747 , B01J19/082 , C08L5/12 , Y10S436/806
摘要： Electrophoretic resolving gel compositions, discontinuous stacking gel systems containing these and other resolving gel compositions, and kits for preparing such gel systems, comprising one or more polysaccharide hydrogels of which agarose is typical wherein at least one of the gels in the resolving gel composition has been derivatized and at least one of the gels in the resolving gel composition has been depolymerized sufficiently to reduce its casting-effective viscosity.
摘要翻译： PCT No.PCT / US90 / 00184 Sec。 371日期1991年6月20日 102（e）1991年6月20日PCT PCT。1990年1月16日。电泳分辨凝胶组合物，含有这些和其它拆分凝胶组合物的不连续堆叠凝胶体系，以及用于制备这种凝胶体系的试剂盒，其包含一种或多种多糖水凝胶其中所述琼脂糖是典型的，其中拆分凝胶组合物中的至少一种凝胶已被衍生化，并且拆分凝胶组合物中的至少一种凝胶已被充分解聚以降低其铸造有效粘度。

3. 发明授权

US4415428A Support for electrophoresis and method of producing same 失效
标题翻译：支持电泳及其生产方法
公开(公告)号：US4415428A
公开(公告)日：1983-11-15
申请号：US342993
申请日：1982-01-27
申请人： Samuel Nochumson , Henry J. Witt
发明人： Samuel Nochumson , Henry J. Witt
IPC分类号： G01N27/447 , B01K5/00
CPC分类号： G01N27/44747 , G01N27/44704
摘要： An electrophoretic support comprising a base plate having on at least one side thereof, a layer of an adherent resin containing ethylenically unsaturated groups capable of undergoing copolymerization with acrylamide. The support provides excellent adhesion for polyacrylamide gels and other electrophoretic media such as agarose.
摘要翻译：一种电泳载体，其包括在其至少一个侧面上具有包含能够与丙烯酰胺共聚的烯属不饱和基团的粘合树脂层的基板。该载体为聚丙烯酰胺凝胶和其他电泳介质如琼脂糖提供优异的粘附性。

4. 发明授权

US08802448B2 Mixed mode ligands 有权
标题翻译：混合模式配体
公开(公告)号：US08802448B2
公开(公告)日：2014-08-12
申请号：US13555484
申请日：2012-07-23
申请人： Hassan Ait-Haddou , Frank Onyemauwa , Jonathan Haigh , Samuel Nochumson
发明人： Hassan Ait-Haddou , Frank Onyemauwa , Jonathan Haigh , Samuel Nochumson
IPC分类号： G01N33/547 , G01N33/538 , G01N33/53
CPC分类号： B01J20/3248 , B01D15/166 , B01D15/3847 , B01J20/24 , B01J20/289 , B01J20/3212 , B01J20/3219 , B01J20/3253 , B01J20/3255 , B01J20/3285 , C07K1/165 , Y02P20/582
摘要： Substrates comprising a solid support, a ligand, and a linker comprising at least one C, O, N, or S atom covalently connecting the solid support to the ligand, are disclosed, along with methods of using and making the substrates, and devices including the substrates.
摘要翻译：公开了包含固体支持物，配体和包含至少一个共价连接固体支持物与配体的C，O，N或S原子的底物，以及使用和制备底物的方法，以及包括底物。

5. 发明授权

US5053332A Agarose beads, preferably incorporating biological materials 失效
标题翻译：琼脂糖珠，优选掺入生物材料
公开(公告)号：US5053332A
公开(公告)日：1991-10-01
申请号：US383520
申请日：1989-07-24
申请人： Richard B. Cook , Richard B. Provonchee , Samuel Nochumson
发明人： Richard B. Cook , Richard B. Provonchee , Samuel Nochumson
IPC分类号： C12N5/00 , C12N11/10
CPC分类号： C12N11/10 , C12N5/0068 , C12N2533/76
摘要： A method for forming agarose beads, which may incorporate biological material at least partially labile at above 40.degree. C., in which an aqueous agarose solution is formed into bead-size portions which are gelled by contacting the portions with a cooled atmosphere, gas, and/or smooth hydrophobic surface, and then collected.
摘要翻译：一种用于形成琼脂糖珠的方法，其可以包含在40℃以上至少部分不稳定的生物材料，其中将琼脂糖水溶液形成为通过使所述部分与冷却的气氛接触而凝胶化的珠粒部分，气体，和/或疏水疏松表面，然后收集。

6. 发明授权

US5552325A Method for separation and recovery of biological materials 失效
标题翻译：生物材料的分离和回收方法
公开(公告)号：US5552325A
公开(公告)日：1996-09-03
申请号：US916801
申请日：1992-07-17
申请人： Samuel Nochumson , Bruce S. Goldberg
发明人： Samuel Nochumson , Bruce S. Goldberg
IPC分类号： G01N1/10 , B01L3/14 , G01N33/49 , G01N33/543 , G01N1/18
CPC分类号： B01L3/5021 , G01N33/491 , G01N33/54366 , Y10T436/25375 , Y10T436/255
摘要： Disclosed is a centrifuge tube containing a porous selection means for selectively separating and recovering biological substances from gels, broths, or solutions containing the same, and thus concentrating them, the tube comprising separate upper and lower containers and means for joining the two containers to form a unitary device, the bottom of the upper container comprising a porous selection means, preferably a membrane containing a particulate substance which will selectively bind the biological substances while allowing unbound substances to pass through from the upper to lower container while being centrifuged. A method for recovering the biological substances in high concentration using the centrifuge tube is also provided, comprising introducing said gels, broths, or solutions containing the desired biological substances, centrifuging the same until the desired substance is bound to the selection means, and eluting the bound material from the selection means to recover the substance in concentrated form.
摘要翻译：公开了一种离心管，其包含多孔选择装置，用于从凝胶，肉汤或含有它们的溶液中选择性分离和回收生物物质，并因此浓缩它们，所述管包括分开的上部和下部容器以及用于连接两个容器以形成上部容器的底部包括多孔选择装置，优选是含有颗粒物质的膜，该颗粒物质将选择性地结合生物物质同时允许未结合的物质在离心时从上到下容器通过。还提供了使用离心管回收高浓度生物物质的方法，包括引入所述凝胶，肉汤或含有所需生物物质的溶液，将其离心，直到所需物质与选择装置结合，来自选择的结合材料意味着以浓缩形式回收物质。

7. 发明授权

US4983268A High gel strength low electroendosmosis agarose 失效
标题翻译：高凝胶强度低电沉积琼脂糖
公开(公告)号：US4983268A
公开(公告)日：1991-01-08
申请号：US389141
申请日：1989-08-03
申请人： Francis H. Kirkpatrick , Kenneth Guiseley , Richard Provonchee , Samuel Nochumson
发明人： Francis H. Kirkpatrick , Kenneth Guiseley , Richard Provonchee , Samuel Nochumson
IPC分类号： B01D57/02 , B01J13/00 , C07K1/24 , C07K1/26 , C08B37/00 , C08B37/12 , C12M1/00 , G01N27/447
CPC分类号： B01D57/02 , C07K1/24 , C07K1/26 , C08B37/0039 , G01N27/44752
摘要： Purified agarose suitable for rapid electrophoresis, characterized by a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a nitrogen content of 0-0.02 wt %. Gels prepared from the agarose exhibit a gel strength at 1.0 wt % concentration of at least 1200 g/cm.sup.2, substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an electroendosmosis (EEO) at 1.0 wt % concentration of 0.05 or less. Agaroses are purified to provide the low EEO material by dissolving agarose or alkali-modified agar in an aqueous medium buffered at a pH of 6.0 to 8.0 and containing no more than 2.0 nM salt as chloride, and precipitating the agarose by contact with a lower alkanol.
摘要翻译：适用于快速电泳的纯化琼脂糖，其特征在于硫酸盐含量小于0.2重量％但大于零，丙酮酸含量为0-0.1重量％，氮含量为0-0.02重量％。从琼脂糖制备的凝胶显示1.0重量％浓度至少为1200g / cm 2的凝胶强度，在0.07M或更少的三乙酸盐缓冲液中基本上不存在DNA结合，以及1.0wt％浓度为0.05的电沉积（EEO）或减。纯化Agaroses以通过将琼脂糖或碱改性琼脂溶解在pH 6.0至8.0的缓冲液中并含有不超过2.0nM的盐作为氯化物，并通过与低级链烷醇接触沉淀琼脂糖而提供低EEO材料。

8. 发明授权

US4504641A Polyacrylamide cross-linked with a polysaccharide resin as electrophoretic gel medium 失效
公开(公告)号：US4504641A
公开(公告)日：1985-03-12
申请号：US542795
申请日：1983-10-17
申请人： Samuel Nochumson
发明人： Samuel Nochumson
IPC分类号： B01D57/02 , C08F251/00 , G01N27/447 , C08L5/02 , C08B37/12
CPC分类号： G01N27/44747 , B01D57/02 , C08F251/00
摘要： An electrophoresis medium, comprising a copolymer of acrylamide and an ethylenically unsaturated resin formed by replacing at least some of the hydroxyl hydrogens in a polysaccharide with an ethylenically unsaturated group, is described.

9. 发明授权

US07732216B2 System and method for testing chromatography media and devices 有权
标题翻译：色谱介质和设备的系统和方法
公开(公告)号：US07732216B2
公开(公告)日：2010-06-08
申请号：US12096033
申请日：2006-11-17
申请人： Samuel Nochumson , Peter Levison
发明人： Samuel Nochumson , Peter Levison
IPC分类号： G01N30/02
CPC分类号： G01N30/89 , G01N30/6091 , G01N2030/889 , G01N2030/528 , G01N2030/527
摘要： Methods for testing the chromatography type and/or the integrity of a chromatography membrane or monolith, preferably, for testing the chromatography type and integrity of a chromatography device comprising a chromatography membrane or chromatography monolith while the membrane or monolith is sealed in a housing, are disclosed.
摘要翻译：用于测试层析类型和/或色谱膜或整料的完整性的方法，优选用于在膜或整体密封在壳体中的情况下测试包括色谱膜或色谱单块的色谱装置的色谱类型和完整性，披露

10. 发明申请

US20060106208A1 Process and equipment for plasmid purfication 有权
标题翻译：用于质粒纯化的工艺和设备
公开(公告)号：US20060106208A1
公开(公告)日：2006-05-18
申请号：US11327987
申请日：2006-01-09
申请人： Samuel Nochumson , Ross Durland , Audrey Yu-Speight , John Welp , Kuoewi Wu , Rexford Hayes
发明人： Samuel Nochumson , Ross Durland , Audrey Yu-Speight , John Welp , Kuoewi Wu , Rexford Hayes
IPC分类号： C07H21/04
CPC分类号： B01D15/327 , B01D15/363 , C12N15/1003 , C12N15/101 , C12N15/1017
摘要： A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinant E. coli cells. Effective, controllable, and economical operation, and consistent low level of host chromosomal DNA in the final plasmid product. Involves a series of new unit operations and devices for cell resuspension, cell lysis, and neutralization.
摘要翻译：可扩展的碱裂解方法，包括从重组大肠杆菌细胞中分离大量（克和千克）质粒DNA的程序和装置。有效，可控和经济的操作，以及最终质粒产物中宿主染色体DNA的低水平。涉及一系列新的单元操作和装置，用于细胞再悬浮，细胞裂解和中和。

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