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1. 发明授权

US07635571B2 Amplified signal in binding assays 有权
标题翻译：结合测定中的扩增信号
公开(公告)号：US07635571B2
公开(公告)日：2009-12-22
申请号：US09732047
申请日：2000-12-07
申请人： Edwin F. Ullman , Rajendra Singh , Steve De Keczer , Dariush Davalian
发明人： Edwin F. Ullman , Rajendra Singh , Steve De Keczer , Dariush Davalian
IPC分类号： G01N33/00
CPC分类号： G01N33/542 , G01N33/531
摘要： Methods are disclosed for determining minute quantities of an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a substrate having an oxidant cleavable linker and a photosensitizer to come into close proximity. The photosensitizer generates singlet oxygen which oxidatively cleaves the linker to form a product which can be detected in a sandwich detection assay such as LOCI. The amount of product detected is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.
摘要翻译：公开了用于确定怀疑含有分析物的介质中微量分析物的方法。一种方法包括处理怀疑含有分析物的介质，使得分析物（如果存在的话）引起具有氧化剂可切割接头和光敏剂的底物接近。光敏剂产生单线态氧，其氧化地切割接头以形成可在三明治检测测定（例如LOCI）中检测的产物。所检测的产物量与培养基中分析物的量有关。还公开了组合物，试剂盒和化合物。

2. 发明授权

US5616719A Photoactive indicator compounds 失效
标题翻译：光敏指示剂化合物
公开(公告)号：US5616719A
公开(公告)日：1997-04-01
申请号：US438154
申请日：1995-05-09
申请人： Dariush Davalian , Rajendra Singh , Edwin F. Ullman
发明人： Dariush Davalian , Rajendra Singh , Edwin F. Ullman
IPC分类号： C07D311/20 , C12Q1/68 , G01N33/533 , G01N33/542 , G01N33/566 , G01N33/569 , G01N33/58 , C07D213/90 , C07C255/00 , C07D311/02
CPC分类号： G01N33/533 , C07D311/20 , G01N33/569 , G01N33/582
摘要： Photoactive indicator compounds of the formula ##STR1##
摘要翻译：光学活性指示剂化合物

3. 发明授权

US5807675A Fluorescent oxygen channeling immunoassays 失效
标题翻译：荧光素氧通道免疫测定
公开(公告)号：US5807675A
公开(公告)日：1998-09-15
申请号：US479743
申请日：1995-06-07
申请人： Dariush Davalian , Rajendra Singh , Edwin F. Ullman
发明人： Dariush Davalian , Rajendra Singh , Edwin F. Ullman
IPC分类号： C07D311/20 , C12Q1/68 , G01N33/533 , G01N33/542 , G01N33/566 , G01N33/569 , G01N33/58 , G01N33/53 , G01N33/543
CPC分类号： G01N33/533 , C07D311/20 , G01N33/569 , G01N33/582
摘要： Methods are disclosed for determining an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a photosensitizer and a photoactive indicator precursor molecule to come into close proximity. The photosensitizer generates singlet oxygen which activates the photoactive indicator precursor to generate a photoactive indicator molecule. Upon irradiation with light the photoactive indicator molecule produces light, which is measured. The amount of light produced by the photoactive indicator is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.
摘要翻译：公开了用于确定怀疑含有分析物的介质中的分析物的方法。一种方法包括在使得分析物（如果存在的话）使光敏剂和光活性指示剂前体分子紧密接近的条件下处理怀疑含有分析物的介质。光敏剂产生单线态氧，其激活光活性指示剂前体以产生光活性指示剂分子。光照射后，光活性指示剂分子产生光，这是测量的。由光活性指示剂产生的光量与介质中分析物的量有关。还公开了组合物，试剂盒和化合物。

4. 发明授权

US06783947B1 Protecting groups for biological labeling 有权
标题翻译：保护群体进行生物标记
公开(公告)号：US06783947B1
公开(公告)日：2004-08-31
申请号：US09393579
申请日：1999-09-09
申请人： Steve de Keczer , Yen Ping Liu , Dariush Davalian , Nurith Kurn , Edwin F. Ullman
发明人： Steve de Keczer , Yen Ping Liu , Dariush Davalian , Nurith Kurn , Edwin F. Ullman
IPC分类号： G01N33546
CPC分类号： G01N33/6842 , C07F9/113 , C07H15/10 , C12Q1/42 , G01N33/6815 , Y02P20/55 , Y10S435/975 , Y10S436/815 , Y10S436/825
摘要： Alpha-haloketones are useful alkylating agents for coupling to sulfhydryl-containing biomolecules. However, they react spontaneously with water, alkali and organic bases and therefore cannot be stored for extended periods of time in aqueous solutions, particularly in the presence of proteins at physiological pH. The present invention provides novel solutions to these problems, however, as novel compounds and compositions comprising protected haloketones are disclosed herein. Methods of preparing and using protected haloketones which are useful in a variety of applications—e.g., in assays and conjugation reactions—are also disclosed herein.
摘要翻译： α-卤代酮是用于与含巯基的生物分子偶联的有用的烷基化剂。然而，它们与水，碱和有机碱发生自发反应，因此不能在水溶液中储存较长时间，特别是在生理pH值的蛋白质存在下。然而，本发明提供了这些问题的新颖解决方案，因为本文公开了包含受保护的卤代酮的新型化合物和组合物。制备和使用可用于各种应用（例如测定和缀合反应）的保护的卤代酮的方法也在本文中公开。

5. 发明授权

US5919641A Homogeneous immunoassays using enzyme inhibitors 失效
公开(公告)号：US5919641A
公开(公告)日：1999-07-06
申请号：US451326
申请日：1995-05-26
申请人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
发明人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
IPC分类号： C12Q1/34 , G01N33/531 , G01N33/535 , G01N33/542 , G01N33/58 , G01N33/53
CPC分类号： G01N33/542 , G01N33/581
摘要： A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described.

6. 发明授权

US6153442A Reagents and methods for specific binding assays 失效
标题翻译：用于特异性结合测定的试剂和方法
公开(公告)号：US6153442A
公开(公告)日：2000-11-28
申请号：US81873
申请日：1998-05-20
申请人： Marcel Rene Pirio , Dariush Davalian , Jacqueline Sadakan Ishkanian , Edwin F. Ullman
发明人： Marcel Rene Pirio , Dariush Davalian , Jacqueline Sadakan Ishkanian , Edwin F. Ullman
IPC分类号： C07D519/00 , C07K14/00 , C12N15/09 , G01N33/532 , G01N33/547 , G01N33/74 , G01N33/78 , G01N33/94 , G01N33/545 , C07D495/04
CPC分类号： G01N33/9453 , G01N33/532 , G01N33/743 , G01N33/78 , Y10S436/815 , Y10S436/817
摘要： The present invention relates to compounds that are bis-biotins. These compounds comprise two biotinyl radicals connected by a chain of atoms, usually at least 16 atoms in length. The bis-biotin is conjugated to a member of a specific binding pair ("sbp member") wherein the chain is not part of the sbp member. Also disclosed are compositions comprising a complex of avidin and a bis-biotin as described above. The compounds and compositions of the invention find use in an assay for an analyte wherein there is employed a reagent system comprising an avidin reagent and a biotin reagent. The improvement of the present invention comprises using as the biotin reagent a bis-biotin as described above. Also disclosed are kits comprising the present bis-biotins and methods of preparing a bis-biotinylated conjugate of a member of a specific binding pair ("sbp member") for use in a specific binding assay.
摘要翻译：本发明涉及双生物素的化合物。这些化合物包括通过原子链连接的两个生物素基团，其通常长度至少为16个原子。双生物素与特异性结合对（“sbp成员”）的成员缀合，其中链不是sbp成员的一部分。还公开了包含如上所述的抗生物素蛋白和双生物素的复合物的组合物。本发明的化合物和组合物用于分析物的测定中，其中使用包含抗生物素蛋白试剂和生物素试剂的试剂系统。本发明的改进包括使用如上所述的双生物素作为生物素试剂。还公开了包含本双双生物素的试剂盒和制备用于特异性结合试验的特异性结合对（“sbp成员”）成员的双生物素化缀合物的方法。

7. 发明授权

US07101682B2 Simultaneous screening of multiple analytes 有权
标题翻译：同时筛选多种分析物
公开(公告)号：US07101682B2
公开(公告)日：2006-09-05
申请号：US11268323
申请日：2005-11-07
申请人： Edwin F. Ullman , Marcel Pirio , Mary C. Ericson , Daniel B. Wagner , Dariush Davalian
发明人： Edwin F. Ullman , Marcel Pirio , Mary C. Ericson , Daniel B. Wagner , Dariush Davalian
IPC分类号： G01N33/53
CPC分类号： G01N33/946 , G01N33/54386 , Y10S435/975 , Y10S436/805 , Y10S436/811 , Y10S436/815 , Y10S436/816 , Y10S436/817 , Y10S436/901
摘要： Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each of the analytes, (iii) for each of the analytes, a first reagent comprising a member of a signal producing system, a ligand and an analyte analog, and (iv) a second reagent comprising a binding partner for the ligand. The binding of the binding partner for the ligand is affected by the presence of an analyte and alters the amount of signal produced by the member of the signal producing system. The signal thus is modulated if one or more of the analytes are present in the sample. The amount of the signal is determined and is related to the presence of one or more of the analytes in the sample. The method may be homogeneous or heterogeneous.
摘要翻译：公开了方法，组合物和试剂盒。该方法旨在确定怀疑含有多种分析物中的任何一种的样品中一种或多种分析物的存在。提供的组合包括在介质（i）样品中，（ii）每种分析物的结合配偶体，（iii）对于每种分析物，包含信号产生系统的成员的第一试剂，配体和分析物类似物，和（iv）包含配体的结合配偶体的第二试剂。配体的结合配偶体的结合受到分析物的存在的影响，并改变由信号产生系统的成员产生的信号量。如果一个或多个分析物存在于样品中，则信号因此被调制。确定信号的量，并与样品中一种或多种分析物的存在相关。该方法可以是均匀的或不均匀的。

8. 发明授权

US5972630A Homogeneous immunoassays using enzyme inhibitors 失效
标题翻译：使用酶抑制剂的均质免疫测定法
公开(公告)号：US5972630A
公开(公告)日：1999-10-26
申请号：US747082
申请日：1991-08-19
申请人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
发明人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
IPC分类号： C12Q1/34 , G01N33/531 , G01N33/535 , G01N33/542 , G01N33/58 , C07K16/00 , C12Q1/00 , C12Q1/54
CPC分类号： G01N33/542 , G01N33/581
摘要： A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described.
摘要翻译：描述了一种用于确定在怀疑含有分析物的样品中作为特异性结合对成员的分析物的存在的方法。该方法包括：在水性介质中将样品，与第一特异性结合对成员结合的酶和与第二特异性结合对成员结合的酶的抑制剂结合在一起，其中每个特异性结合对成员能够结合至分析物或与分析物互补的特异性结合对成员; 分析培养基中所述酶的活性; 并将活性与介质中存在的分析物的量相关联。还描述了物质和试剂盒的组成。

9. 发明授权

US06797481B1 Simultaneous screening of multiple analytes 有权
标题翻译：同时筛选多种分析物
公开(公告)号：US06797481B1
公开(公告)日：2004-09-28
申请号：US09691383
申请日：2000-10-17
申请人： Edwin F. Ullman , Marcel Pirio , Mary C. Ericson , Daniel B. Wagner , Dariush Davalian
发明人： Edwin F. Ullman , Marcel Pirio , Mary C. Ericson , Daniel B. Wagner , Dariush Davalian
IPC分类号： G01N3353
CPC分类号： G01N33/946 , G01N33/54386 , Y10S435/975 , Y10S436/805 , Y10S436/811 , Y10S436/815 , Y10S436/816 , Y10S436/817 , Y10S436/901
摘要： Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each of the analytes, (iii) for each of the analytes, a first reagent comprising a member of a signal producing system, a ligand and an analyte analog, and (iv) a second reagent comprising a binding partner for the ligand. The binding of the binding partner for the ligand is affected by the presence of an analyte and alters the amount of signal produced by the member of the signal producing system. The signal thus is modulated if one or more of the analytes are present in the sample. The amount of the signal is determined and is related to the presence of one or more of the analytes in the sample. The method may be homogeneous or heterogeneous.
摘要翻译：公开了方法，组合物和试剂盒。该方法旨在确定怀疑含有多种分析物中的任何一种的样品中一种或多种分析物的存在。提供的组合包括在介质（i）样品中，（ii）每种分析物的结合配偶体，（iii）对于每种分析物，包含信号产生系统的成员的第一试剂，配体和分析物类似物，和（iv）包含配体的结合配偶体的第二试剂。配体的结合配偶体的结合受到分析物的存在的影响，并改变由信号产生系统的成员产生的信号量。如果一个或多个分析物存在于样品中，则信号因此被调制。确定信号的量，并与样品中一种或多种分析物的存在相关。该方法可以是均匀的或不均匀的。

10. 发明授权

US6054303A Cyclosporin immunoassay 失效
公开(公告)号：US6054303A
公开(公告)日：2000-04-25
申请号：US401827
申请日：1995-03-10
申请人： Dariush Davalian , Maureen H. Beresini , Svetlana Alexander , Mae Wan-Leng Hu , Edwin F. Ullman
发明人： Dariush Davalian , Maureen H. Beresini , Svetlana Alexander , Mae Wan-Leng Hu , Edwin F. Ullman
IPC分类号： A61K38/00 , A61K39/385 , A61K39/395 , C07K1/06 , C07K1/113 , C07K7/64 , C07K14/00 , C07K16/00 , C07K16/44 , C07K19/00 , C12Q1/32 , G01N33/53 , G01N33/535 , G01N33/94 , C12N9/96 , G01N33/533
CPC分类号： G01N33/5306 , C07K16/44 , C07K7/645 , G01N33/535 , G01N33/9493 , Y10S435/962 , Y10S436/815 , Y10S436/825 , Y10S436/829 , Y10S530/807
摘要： A method of measuring the amount of cyclosporin in a sample suspected of containing cyclosporin is disclosed. A method of inactivating interfering cross-reactive material in an assay for measuring the amount of cyclosporin in a sample suspected of containing cyclosporin is also disclosed. Compositions wherein cyclosporin is conjugated to an immunogenic carrier or a label, optionally through a linking group, at an alanine nitrogen atom of the cyclic backbone of cyclosporin are also disclosed. Compositions wherein atiocyclosporin is conjugated, optionally through a linking group, to an immunogenic carrier or a label are also disclosed. Where cyclosporin is conjugated to an immunogenic carrier, the conjugates may be used as immunogens for the preparation of antibodies which are capable of recognizing cyclosporin. Where atiocyclosporin is conjugated to an immunogenic carrier, the conjugates may be used as immunogens for the preparation of antibodies which are capable of recognizing interfering cross-reactive material but substantially incapable of recognizing cyclosporin or cyclosporin-label conjugates. Where cyclosporin is conjugated to a label, the conjugates may be used as part of a signal producing system in cyclosporin assays. Both the antibodies and label conjugates are useful in the disclosed assay methods.

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