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1. 发明授权

US5919641A Homogeneous immunoassays using enzyme inhibitors 失效
公开(公告)号：US5919641A
公开(公告)日：1999-07-06
申请号：US451326
申请日：1995-05-26
申请人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
发明人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
IPC分类号： C12Q1/34 , G01N33/531 , G01N33/535 , G01N33/542 , G01N33/58 , G01N33/53
CPC分类号： G01N33/542 , G01N33/581
摘要： A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described.

2. 发明授权

US5972630A Homogeneous immunoassays using enzyme inhibitors 失效
标题翻译：使用酶抑制剂的均质免疫测定法
公开(公告)号：US5972630A
公开(公告)日：1999-10-26
申请号：US747082
申请日：1991-08-19
申请人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
发明人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
IPC分类号： C12Q1/34 , G01N33/531 , G01N33/535 , G01N33/542 , G01N33/58 , C07K16/00 , C12Q1/00 , C12Q1/54
CPC分类号： G01N33/542 , G01N33/581
摘要： A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described.
摘要翻译：描述了一种用于确定在怀疑含有分析物的样品中作为特异性结合对成员的分析物的存在的方法。该方法包括：在水性介质中将样品，与第一特异性结合对成员结合的酶和与第二特异性结合对成员结合的酶的抑制剂结合在一起，其中每个特异性结合对成员能够结合至分析物或与分析物互补的特异性结合对成员; 分析培养基中所述酶的活性; 并将活性与介质中存在的分析物的量相关联。还描述了物质和试剂盒的组成。

3. 发明授权

US5624809A Device for immunochromatographic analysis 失效
标题翻译：免疫层析分析装置
公开(公告)号：US5624809A
公开(公告)日：1997-04-29
申请号：US436053
申请日：1995-05-05
申请人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
发明人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
IPC分类号： G01N33/543 , G01N33/558 , G01N33/548
CPC分类号： G01N33/54366 , G01N33/54386 , G01N33/558 , Y10S435/805 , Y10S435/97 , Y10S435/975 , Y10T436/19
摘要： Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present. The distance the component has migrated into the second zone or the difference in the distances the medium and the component have migrated into the second zone is determined, the distance or the difference being related to the amount of the component in the liquid medium or the amount of the reagent.
摘要翻译：公开了用于进行测定的方法。一种这样的方法包括组合提供第一吸引构件区域（“第一区域”）和含有组分的液体介质。第一区域非扩散地结合与组分反应的试剂。选择条件，其中液体介质和其中包含的成分的至少一部分穿过所有第一区域并通过毛细管迁移迁移到第二吸水构件区域（“第二区域”）中。第二区域具有与第一区域不同的组成，并且不能特异性地结合该组分，除了当要分析物被检测时，该方法还包括使试剂与第一吸水构件区域相关的量结合的分析物存在。确定组件已经迁移到第二区域的距离或介质和组件迁移到第二区域的距离差，距离或差异与液体介质中组分的量或量的试剂。

4. 发明授权

US5039607A Method for immunochromatographic analysis 失效
标题翻译：免疫色谱分析方法
公开(公告)号：US5039607A
公开(公告)日：1991-08-13
申请号：US194708
申请日：1988-05-17
申请人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
发明人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N33/54386 , G01N33/558 , Y10S435/97 , Y10S435/971 , Y10S435/972 , Y10S435/975 , Y10S436/807 , Y10S436/81 , Y10S436/815 , Y10S436/816 , Y10S436/819 , Y10S436/822
摘要： A method is disclosed for conducting an assay for an analyte. The method comprises causing a specific binding pair member in a first aqueous medium to become bound to a first bibulous member by contacting a portion of the first bibulous member with the medium. The first bibulous member is in liquid receiving relationship with an absorbent member. The contacting is carried out under conditions wherein the first medium traverses the first bibulous member and at least a portion of the absorbent member by capillary action. The method further comprises causing the first bibulous member to come into liquid receiving relationship with a second bibulous member. A reagent in a second aqueous medium is absorbed by and preferably becomes a non-diffusively bound to the second bibulous member in relation to the presence of analyte in the first medium. This is accomplished by contacting a portion of the first bibulous member with the second aqueous medium under conditions wherein the second aqueous medium traverses the first bibulous member by capillary action and at least a portion of the second bibulous member. Next, the presence of the reagent on at least a portion of the second bibulous member is determined.

5. 发明授权

US5468647A Method for immunochromatographic analysis 失效
标题翻译：免疫色谱分析方法
公开(公告)号：US5468647A
公开(公告)日：1995-11-21
申请号：US299860
申请日：1994-09-01
申请人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
发明人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N33/54386 , G01N33/558 , Y10S435/97 , Y10S435/971 , Y10S435/972 , Y10S435/975 , Y10S436/807 , Y10S436/81 , Y10S436/815 , Y10S436/816 , Y10S436/819 , Y10S436/822
摘要： A method is disclosed for conducting an assay for an analyte. The method comprises causing a specific binding pair member in a first aqueous medium to become bound to a first bibulous member by contacting a portion of the first bibulous member with the medium. The first bibulous member is in liquid receiving relationship with an absorbent member. The contacting is carried out under conditions wherein the first medium traverses the first bibulous member and at least a portion of the absorbent member by capillary action. The method further comprises causing the first bibulous member to come into liquid receiving relationship with a second bibulous member. A reagent in a second aqueous medium is absorbed by and preferably becomes non-diffusively bound to the second bibulous member in relation to the presence of analyte in the first medium. This is accomplished by contacting a portion of the first bibulous member with the second aqueous medium under conditions wherein the second aqueous medium traverses the first bibulous member by capillary action and at least a portion of the second bibulous member. Next, the presence of the reagent on at least a portion of the second bibulous member is determined.
摘要翻译：公开了用于进行分析物测定的方法。该方法包括使第一含水介质中的特定结合对构件通过使第一吸水构件的一部分与介质接触而结合到第一吸水构件。第一吸水构件与吸收构件处于液体接收关系。接触是在第一介质通过毛细管作用横穿第一吸引构件和吸收体的至少一部分的条件下进行的。该方法还包括使第一吸水构件与第二吸水构件进行液体接收关系。相对于第一介质中的分析物的存在，第二水性介质中的试剂被第二吸水构件吸收并优选变得非扩散地结合。这是通过使第一吸水构件的一部分与第二水性介质在第二水性介质通过毛细管作用横穿第一吸水构件和第二吸水构件的至少一部分的条件下接触来实现的。接下来，确定在第二吸水构件的至少一部分上存在试剂。

6. 发明授权

US5164294A Method for immunochromatographic analysis 失效
标题翻译：免疫色谱分析方法
公开(公告)号：US5164294A
公开(公告)日：1992-11-17
申请号：US376723
申请日：1989-07-07
申请人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
发明人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N33/558 , G01N33/54386 , Y10S435/805 , Y10S435/97 , Y10S436/81
摘要： Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present. The distance the component has migrated into the second zone or the difference in the distances the medium and the component have migrated into the second zone is determined, the distance or the difference being related to the amount of the component in the liquid medium or the amount of the reagent.
摘要翻译：公开了用于进行测定的方法。一种这样的方法包括组合提供第一吸引构件区域（“第一区域”）和含有组分的液体介质。第一区域非扩散地结合与组分反应的试剂。选择条件，其中液体介质和其中包含的成分的至少一部分穿过所有第一区域并通过毛细管迁移迁移到第二吸水构件区域（“第二区域”）中。第二区域具有与第一区域不同的组成，并且不能特异性地结合该组分，除了当要分析物被检测时，该方法还包括使试剂与第一吸水构件区域相关的量结合的分析物存在。确定组件已经迁移到第二区域的距离或介质和组件迁移到第二区域的距离差，距离或差异与液体介质中组分的量或量的试剂。

7. 发明授权

US5451507A Method for immunochromatographic analysis 失效
公开(公告)号：US5451507A
公开(公告)日：1995-09-19
申请号：US241307
申请日：1994-05-10
申请人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
发明人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N33/54366 , G01N33/54386 , G01N33/558 , Y10S435/805 , Y10S435/97 , Y10S435/975 , Y10T436/19
摘要： Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present. The distance the component has migrated into the second zone or the difference in the distances the medium and the component have migrated into the second zone is determined, the distance or the difference being related to the amount of the component in the liquid medium or the amount of the reagent.

8. 发明授权

US5334513A Method for immunochromatographic analysis 失效
标题翻译：免疫色谱分析方法
公开(公告)号：US5334513A
公开(公告)日：1994-08-02
申请号：US940137
申请日：1992-09-03
申请人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
发明人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
IPC分类号： G01N33/543 , G01N33/558 , G01N30/00
CPC分类号： G01N33/54366 , G01N33/54386 , G01N33/558 , Y10S435/805 , Y10S435/97 , Y10S435/975 , Y10T436/19
摘要： Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present. The distance the component has migrated into the second zone or the difference in the distances the medium and the component have migrated into the second zone is determined, the distance or the difference being related to the amount of the component in the liquid medium or the amount of the reagent.
摘要翻译：公开了用于进行测定的方法。一种这样的方法包括组合提供第一吸引构件区域（“第一区域”）和含有组分的液体介质。第一区域非扩散地结合与组分反应的试剂。选择条件，其中液体介质和其中包含的成分的至少一部分穿过所有第一区域并通过毛细管迁移迁移到第二吸水构件区域（“第二区域”）中。第二区域具有与第一区域不同的组成，并且不能特异性地结合该组分，除了当要分析物被检测时，该方法还包括使试剂与第一吸水构件区域相关的量结合的分析物存在。确定组件已经迁移到第二区域的距离或介质和组件迁移到第二区域的距离差，距离或差异与液体介质中组分的量或量的试剂。

9. 发明授权

US5248619A Device and kit for immunochromatographic analysis 失效
标题翻译：用于免疫荧光分析的装置和工具包
公开(公告)号：US5248619A
公开(公告)日：1993-09-28
申请号：US714791
申请日：1991-06-13
申请人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
发明人： Carl N. Skold , Armen B. Shanafelt , Vartan Ghazarossian , Edwin F. Ullman
IPC分类号： G01N33/543 , G01N33/558
CPC分类号： G01N33/54386 , G01N33/558 , Y10S435/97 , Y10S435/971 , Y10S435/972 , Y10S435/975 , Y10S436/807 , Y10S436/81 , Y10S436/815 , Y10S436/816 , Y10S436/819 , Y10S436/822
摘要： A method is disclosed for conducting an assay for an analyte. The method comprises causing a specific binding pair member in a first aqueous medium to become bound to a first bibulous member by contacting a portion of the first bibulous member with the medium. The first bibulous member is in liquid receiving relationship with an absorbent member. The contacting is carried out under conditions wherein the first medium traverses the first bibulous member and at least a portion of the absorbent member by capillary action. The method further comprises causing the first bibulous member to come into liquid receiving relationship with a second bibulous member. A reagent in a second aqueous medium is absorbed by and preferably becomes non-diffusively bound to the second bibulous member in relation to the presence of analyte in the first medium. This is accomplished by contacting a portion of the first bibulous member with the second aqueous medium under conditions wherein the second aqueous medium traverses the first bibulous member by capillary action and at least a portion of the second bibulous member. Next, the presence of the reagent on at least a portion of the second bibulous member is determined.

10. 发明授权

US5686253A Method of stabilizing enzyme conjugates 失效
标题翻译：稳定酶缀合物的方法
公开(公告)号：US5686253A
公开(公告)日：1997-11-11
申请号：US450744
申请日：1995-05-25
申请人： Carl N. Skold , Margaret Henson , Thomas Michael Houts , Ian Gibbons
发明人： Carl N. Skold , Margaret Henson , Thomas Michael Houts , Ian Gibbons
IPC分类号： C12N5/10 , C12N9/96 , C12N15/02 , C12N15/09 , C12P21/08 , C12Q1/26 , C12Q1/28 , C12Q1/32 , C12Q1/68 , G01N33/535 , G01N33/542 , G01N33/573 , G01N33/577
CPC分类号： G01N33/542 , C12N9/96 , G01N33/535 , Y10S435/963 , Y10S435/975 , Y10S436/822
摘要： A method is disclosed for stabilizing a conjugate of an enzyme and a member of a specific binding pair (enzyme conjugate). The method comprises the step of combining the enzyme conjugate with an effective amount of an antibody for the enzyme where the antibody does not substantially inhibit the activity of the enzyme. The invention has application to assays for the determination of an analyte wherein enzyme conjugates are employed. The improvement comprises employing as a reagent in the assay an immune complex of an enzyme conjugate and an antibody for the enzyme where the antibody does not substantially inhibit the activity of the enzyme. Compositions comprising such an immune complex and kits comprising such an immune complex in packaged combination with other assay reagents are also disclosed.
摘要翻译：公开了用于稳定酶和特异性结合对（酶结合物）成员的缀合物的方法。该方法包括将酶结合物与酶的有效量的抗体组合的步骤，其中抗体基本上不抑制酶的活性。本发明应用于测定其中使用酶缀合物的分析物的测定。该改进包括在测定中使用酶缀合物的免疫复合物和酶的抗体，其中抗体基本上不抑制酶的活性。还公开了包含这种免疫复合物的组合物和包含与其它测定试剂的包装组合中的这种免疫复合物的试剂盒。

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