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1. 发明授权

US08852867B2 Nucleic acid amplification procedure using RNA and DNA composite primers 有权
标题翻译：使用RNA和DNA复合引物的核酸扩增程序
公开(公告)号：US08852867B2
公开(公告)日：2014-10-07
申请号：US13103865
申请日：2011-05-09
申请人： Nurith Kurn , Shenglong Wang
发明人： Nurith Kurn , Shenglong Wang
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6865
摘要： The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA-containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
摘要翻译：本发明提供使用含有单链RNA的引物扩增多核苷酸序列的方法。该方法使用能够切割单链RNA（例如RNA酶I）的酶在加入第二种含RNA引物之前降解第一种含有RNA的引物。本发明还提供了用于实施扩增方法的组合物和试剂盒，以及使用扩增产物的方法。

2. 发明授权

US08465950B2 Global amplification using a randomly primed composite primer 有权
公开(公告)号：US08465950B2
公开(公告)日：2013-06-18
申请号：US13349927
申请日：2012-01-13
申请人： Nurith Kurn , Shenglong Wang
发明人： Nurith Kurn , Shenglong Wang
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2537/143 , C12Q2525/203 , C12Q2525/179
摘要： The invention relates to the field of polynucleotide amplification. More particularly, the invention provides methods, compositions and kits for amplification of (i.e., making multiple copies of) a multiplicity of different polynucleotide template sequences using a randomly primed RNA/DNA composite primer.

3. 发明授权

US08143001B2 Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids 有权
标题翻译：分析核酸甲基化状态的方法和核酸的断裂，标记和固定方法
公开(公告)号：US08143001B2
公开(公告)日：2012-03-27
申请号：US11948784
申请日：2007-11-30
申请人： Nurith Kurn , Geoffrey A. Dafforn
发明人： Nurith Kurn , Geoffrey A. Dafforn
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , C12P19/34 , C12Q2525/119 , C12Q2521/531
摘要： The invention relates to methods for analysis of nucleic acid methylation status, and fragmentation and/or labeling and/or immobilization of nucleic acids. More particularly, the invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids comprising labeling and/or cleavage and/or immobilization at abasic sites.
摘要翻译：本发明涉及用于分析核酸甲基化状态以及核酸的断裂和/或标记和/或固定化的方法。更具体地说，本发明涉及核酸的分离和/或标记和/或固定化的方法，包括在非碱性位点标记和/或切割和/或固定化。

4. 发明申请

US20120028310A1 ISOTHERMAL NUCLEIC ACID AMPLIFICATION METHODS AND COMPOSITIONS 审中-公开
标题翻译：同位素核酸扩增方法和组合物
公开(公告)号：US20120028310A1
公开(公告)日：2012-02-02
申请号：US13211996
申请日：2011-08-17
申请人： Nurith Kurn , Shenglong Wang
发明人： Nurith Kurn , Shenglong Wang
IPC分类号： C12P19/34
CPC分类号： C12Q1/686 , C12Q2525/191 , C12Q2531/101 , C12Q2537/1373 , C12Q2525/301
摘要： Methods and compositions are provided related to the amplification of target polynucleotide sequences as well as total RNA and total DNA amplification. In some embodiments, the methods and compositions also allow for the immobilization and capture of target polynucleotides with defined 3′ and or 5′ sequences to solid surfaces. The polynucleotides attached to the solid surfaces can be amplified or eluted for downstream processing. In some cases, nucleotides attached to solid surfaces can be used for high throughput sequencing of nucleotide sequences related to target DNA or target RNA.
摘要翻译：提供了与靶多核苷酸序列的扩增以及总RNA和总DNA扩增相关的方法和组合物。在一些实施方案中，所述方法和组合物还允许将具有限定的3'和/或5'序列的靶多核苷酸固定并捕获到固体表面。连接到固体表面的多核苷酸可以被扩增或洗脱用于下游处理。在某些情况下，连接到固体表面的核苷酸可用于与靶DNA或靶RNA相关的核苷酸序列的高通量测序。

5. 发明授权

US07771946B2 Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences 有权
标题翻译：用于核酸序列的单引物线性等温扩增的方法，试剂盒和组合物
公开(公告)号：US07771946B2
公开(公告)日：2010-08-10
申请号：US12020434
申请日：2008-01-25
申请人： Nurith Kurn
发明人： Nurith Kurn
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6853 , C12Q2531/119
摘要： The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
摘要翻译：本发明提供了RNA等温扩增的方法。该方法特别适用于扩增样品中的多种RNA物种。该方法采用复合引物，第二引物和链置换，以产生包含与感兴趣的RNA序列互补的序列的DNA产物的多个拷贝。另一方面，该方法采用单一引物（其是复合引物）和链置换以产生包含与感兴趣的RNA序列互补的序列的DNA产物的多个拷贝。在一些实施方案中，包括转录步骤以产生感兴趣的RNA序列的有义RNA的多个拷贝。该方法可用于制备用于分析生物样品中细胞基因表达的核酸文库和底物。本发明还提供了用于实施扩增方法的组合物和试剂盒，以及使用扩增产物的方法。

6. 发明申请

US20100167354A1 METHODS AND COMPOSITIONS FOR AMPLIFICATION OF RNA SEQUENCES 有权
标题翻译： RNA序列扩增的方法和组合物
公开(公告)号：US20100167354A1
公开(公告)日：2010-07-01
申请号：US12615958
申请日：2009-11-10
申请人： Nurith Kurn
发明人： Nurith Kurn
IPC分类号： C12P19/34 , C07H21/02 , C12N9/10
CPC分类号： C12Q1/6853 , C12Q2531/119
摘要： The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
摘要翻译：本发明提供了RNA等温扩增的方法。该方法特别适用于扩增样品中的多种RNA物种。该方法采用复合引物，第二引物和链置换，以产生包含与感兴趣的RNA序列互补的序列的DNA产物的多个拷贝。另一方面，该方法采用单一引物（其是复合引物）和链置换以产生包含与感兴趣的RNA序列互补的序列的DNA产物的多个拷贝。在一些实施方案中，包括转录步骤以产生感兴趣的RNA序列的有义RNA的多个拷贝。该方法可用于制备用于分析生物样品中细胞基因表达的核酸文库和底物。本发明还提供了用于实施扩增方法的组合物和试剂盒，以及使用扩增产物的方法。

7. 发明申请

US20090203085A1 Isothermal Nucleic Acid Amplification Methods and Compositions 有权
标题翻译：等温核酸扩增方法和组成
公开(公告)号：US20090203085A1
公开(公告)日：2009-08-13
申请号：US12370534
申请日：2009-02-12
申请人： Nurith KURN , Shenglong Wang
发明人： Nurith KURN , Shenglong Wang
IPC分类号： C12P19/34
CPC分类号： C12Q1/686 , C12Q2525/191 , C12Q2531/101 , C12Q2537/1373 , C12Q2525/301
摘要： Methods and compositions are provided related to the amplification of target polynucleotide sequences as well as total RNA and total DNA amplification. In some embodiments, the methods and compositions also allow for the immobilization and capture of target polynucleotides with defined 3′ and or 5′ sequences to solid surfaces. The polynucleotides attached to the solid surfaces can be amplified or eluted for downstream processing. In some cases, nucleotides attached to solid surfaces can be used for high throughput sequencing of nucleotide sequences related to target DNA or target RNA.
摘要翻译：提供了与靶多核苷酸序列的扩增以及总RNA和总DNA扩增相关的方法和组合物。在一些实施方案中，所述方法和组合物还允许将具有限定的3'和/或5'序列的靶多核苷酸固定并捕获到固体表面。连接到固体表面的多核苷酸可以被扩增或洗脱用于下游处理。在某些情况下，连接到固体表面的核苷酸可用于与靶DNA或靶RNA相关的核苷酸序列的高通量测序。

8. 发明申请

US20050208538A1 Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids 审中-公开
标题翻译：分析核酸甲基化状态的方法和核酸的断裂，标记和固定方法
公开(公告)号：US20050208538A1
公开(公告)日：2005-09-22
申请号：US11026280
申请日：2004-12-29
申请人： Nurith Kurn , Geoffrey Dafforn
发明人： Nurith Kurn , Geoffrey Dafforn
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6827 , C12P19/34 , C12Q2525/119 , C12Q2521/531
摘要： The invention relates to methods for analysis of nucleic acid methylation status, and fragmentation and/or labeling and/or immobilization of nucleic acids. More particularly, the invention relates to methods for fragmentation and/or labeling and/or immobilization of nucleic acids comprising labeling and/or cleavage and/or immobilization at abasic sites.
摘要翻译：本发明涉及用于分析核酸甲基化状态以及核酸的断裂和/或标记和/或固定化的方法。更具体地说，本发明涉及核酸的分离和/或标记和/或固定化的方法，包括在非碱性位点标记和/或切割和/或固定化。

9. 发明授权

US06692918B2 Methods and compositions for linear isothermal amplification of polynucleotide sequences 有权
标题翻译：用于多核苷酸序列的线性等温扩增的方法和组合物
公开(公告)号：US06692918B2
公开(公告)日：2004-02-17
申请号：US09990531
申请日：2001-11-21
申请人： Nurith Kurn
发明人： Nurith Kurn
IPC分类号： C12Q168
CPC分类号： C12Q1/6844 , C12Q1/6865 , C12Q2537/1373 , C12Q2531/101 , C12Q2521/301 , C12Q2525/186 , C12Q2525/143
摘要： The present invention provides novel isothermal, single primer linear nucleic acid amplification methods. Methods for amplifying complementary DNA using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification products.
摘要翻译：本发明提供了新型等温，单引物线性核酸扩增方法。提供了使用复合引物扩增互补DNA的方法，引物延伸，链置换和任选的终止序列。还提供了使用复合引物扩增正义RNA的方法，引物延伸，链置换，任选的模板切换，启动子寡核苷酸和转录。本发明还提供了用于实施所述方法的组合物和试剂盒，以及使用扩增产物的方法。

10. 发明授权

US6013439A Detection of differences in nucleic acids 失效
公开(公告)号：US6013439A
公开(公告)日：2000-01-11
申请号：US771623
申请日：1996-12-20
申请人： Alla Lishanski , Nurith Kurn , Edwin F. Ullman
发明人： Alla Lishanski , Nurith Kurn , Edwin F. Ullman
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6823 , C12Q1/6813 , C12Q1/6818 , C12Q1/6827 , Y10S435/808
摘要： A method is disclosed for detecting the presence of a difference between two related nucleic acid sequences. In the method a complex is formed comprising both strands of each sequence. Each member of at least one pair of non-complementary strands within the complex have labels. The association of the labels as part of the complex is determined as an indication of the presence of a difference between the two related sequences. The complex generally comprises a Holliday junction. In one aspect a medium suspected of containing said two related nucleic acid sequences is treated to provide partial duplexes having non-complementary tailed portions at one end. The double stranded portions of the partial duplexes are identical except for said difference. One of the strands of one of the partial duplexes is complementary to one of the strands of the other of the partial duplexes and the other of the strands of one of the partial duplexes is complementary to the other of the strands of the other of the partial duplexes. The medium is subjected to conditions that permit the binding of the tailed portions of the partial duplexes to each other. If there is a difference in the related nucleic acid sequences, a stable complex is formed comprising a Holliday junction. If no difference exists, the complex dissociates into duplexes. A determination is made whether the stable complex is formed, the presence thereof indicating the presence of the related nucleic acid sequences. The method has application in detecting the presence of a mutation in a target sequence or in detecting the target sequence itself.

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