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1. 发明授权

US06783947B1 Protecting groups for biological labeling 有权
标题翻译：保护群体进行生物标记
公开(公告)号：US06783947B1
公开(公告)日：2004-08-31
申请号：US09393579
申请日：1999-09-09
申请人： Steve de Keczer , Yen Ping Liu , Dariush Davalian , Nurith Kurn , Edwin F. Ullman
发明人： Steve de Keczer , Yen Ping Liu , Dariush Davalian , Nurith Kurn , Edwin F. Ullman
IPC分类号： G01N33546
CPC分类号： G01N33/6842 , C07F9/113 , C07H15/10 , C12Q1/42 , G01N33/6815 , Y02P20/55 , Y10S435/975 , Y10S436/815 , Y10S436/825
摘要： Alpha-haloketones are useful alkylating agents for coupling to sulfhydryl-containing biomolecules. However, they react spontaneously with water, alkali and organic bases and therefore cannot be stored for extended periods of time in aqueous solutions, particularly in the presence of proteins at physiological pH. The present invention provides novel solutions to these problems, however, as novel compounds and compositions comprising protected haloketones are disclosed herein. Methods of preparing and using protected haloketones which are useful in a variety of applications—e.g., in assays and conjugation reactions—are also disclosed herein.
摘要翻译： α-卤代酮是用于与含巯基的生物分子偶联的有用的烷基化剂。然而，它们与水，碱和有机碱发生自发反应，因此不能在水溶液中储存较长时间，特别是在生理pH值的蛋白质存在下。然而，本发明提供了这些问题的新颖解决方案，因为本文公开了包含受保护的卤代酮的新型化合物和组合物。制备和使用可用于各种应用（例如测定和缀合反应）的保护的卤代酮的方法也在本文中公开。

2. 发明授权

US5914230A Homogeneous amplification and detection of nucleic acids 失效
标题翻译：核酸的均相扩增和检测
公开(公告)号：US5914230A
公开(公告)日：1999-06-22
申请号：US771624
申请日：1996-12-20
申请人： Yen Ping Liu , Rajesh D. Patel , Nurith Kurn , Claire Lin , Samuel J. Rose , Edwin F. Ullman
发明人： Yen Ping Liu , Rajesh D. Patel , Nurith Kurn , Claire Lin , Samuel J. Rose , Edwin F. Ullman
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566 , C12P19/34
CPC分类号： C12Q1/6816 , C12Q1/6813 , C12Q1/6818 , C12Q1/686
摘要： The present invention relates to a method for detecting or amplifying and detecting a target polynucleotide sequence. The method comprises providing in combination (i) a medium suspected of containing the target polynucleotide sequence, (ii) all reagents required for conducting an amplification of the target polynucleotide sequence when amplification is desired, and (iii) two oligonucleotide probes capable of binding to a single strand of the product of the amplification. At least one of the probes has two sequences that either (i) are non-contiguous and bind to contiguous or non-contiguous sites on the single strand or (ii) can bind to non-contiguous sites on the single strand. Each probe may contain a label. The combination is subjected to conditions for amplifying the target polynucleotide sequence. Next, the combination is subjected to conditions under which both of the probes hybridize to one of the strands to form a termolecular complex, which is detected by means of the label.
摘要翻译：本发明涉及检测或扩增和检测靶多核苷酸序列的方法。所述方法包括组合（i）怀疑含有靶多核苷酸序列的培养基，（ii）当需要扩增时进行靶多核苷酸序列扩增所需的所有试剂，和（iii）能够结合到单链的扩增产物。至少一个探针具有以下两个序列：（i）不连续并且结合单链上的连续或非连续位点，或（ii）可以结合单链上的非连续位点。每个探针可能包含一个标签。将该组合进行扩增靶多核苷酸序列的条件。接下来，将该组合进行两个探针与其中一条链杂交以形成通过标记检测的分子复合物的条件。

3. 发明授权

US6013439A Detection of differences in nucleic acids 失效
公开(公告)号：US6013439A
公开(公告)日：2000-01-11
申请号：US771623
申请日：1996-12-20
申请人： Alla Lishanski , Nurith Kurn , Edwin F. Ullman
发明人： Alla Lishanski , Nurith Kurn , Edwin F. Ullman
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6823 , C12Q1/6813 , C12Q1/6818 , C12Q1/6827 , Y10S435/808
摘要： A method is disclosed for detecting the presence of a difference between two related nucleic acid sequences. In the method a complex is formed comprising both strands of each sequence. Each member of at least one pair of non-complementary strands within the complex have labels. The association of the labels as part of the complex is determined as an indication of the presence of a difference between the two related sequences. The complex generally comprises a Holliday junction. In one aspect a medium suspected of containing said two related nucleic acid sequences is treated to provide partial duplexes having non-complementary tailed portions at one end. The double stranded portions of the partial duplexes are identical except for said difference. One of the strands of one of the partial duplexes is complementary to one of the strands of the other of the partial duplexes and the other of the strands of one of the partial duplexes is complementary to the other of the strands of the other of the partial duplexes. The medium is subjected to conditions that permit the binding of the tailed portions of the partial duplexes to each other. If there is a difference in the related nucleic acid sequences, a stable complex is formed comprising a Holliday junction. If no difference exists, the complex dissociates into duplexes. A determination is made whether the stable complex is formed, the presence thereof indicating the presence of the related nucleic acid sequences. The method has application in detecting the presence of a mutation in a target sequence or in detecting the target sequence itself.

4. 发明授权

US5840508A Compositions and methods for removal of detergents 失效
公开(公告)号：US5840508A
公开(公告)日：1998-11-24
申请号：US455424
申请日：1995-05-31
申请人： Arthur C. Switchenko , Nurith Kurn , Christian Neukom , Marcel Pirio , Donald E. Berger, Jr. , Edwin F. Ullman
发明人： Arthur C. Switchenko , Nurith Kurn , Christian Neukom , Marcel Pirio , Donald E. Berger, Jr. , Edwin F. Ullman
IPC分类号： G01N33/531 , A61K39/00 , B01F17/00 , C07C323/12 , C07C323/14 , C07K1/32 , C07K14/22 , C07K14/295 , G01N33/569 , G01N33/68 , G01N33/92
CPC分类号： B01F17/0064 , B01F17/0021 , B01F17/0057 , C07C323/14 , C07K1/145 , C07K1/32 , C07K14/22 , C07K14/295 , G01N33/56927 , G01N33/6803 , G01N33/92 , Y10S435/961 , Y10S435/962 , Y10S435/967 , Y10S436/825 , Y10T436/10 , Y10T436/107497 , Y10T436/25 , Y10T436/25125
摘要： Compounds having detergent properties are disclosed. When a modifying reagent is brought into contact with these compounds, the detergent properties are decreased. These compounds are useful, for example, as solubilizing agents for microbial antigens and/or antibodies and for reversibly wetting hydrophobic surfaces. Accordingly, methods are disclosed for increasing the hydrophilic properties of a material, such as a microbial antigen and/or antibody, the methods generally comprising the steps of contacting the material with the compound having detergent properties and a modifiable group, and modifying the compound with a modifying reagent. Kits are also disclosed for use in accordance with this methodology.

5. 发明授权

US5260222A Device and method for completing a fluidic circuit which employs a liquid expandable piece of bibulous material 失效
标题翻译：用于完成使用吸水材料的液体可膨胀件的流体回路的装置和方法
公开(公告)号：US5260222A
公开(公告)日：1993-11-09
申请号：US874662
申请日：1992-04-27
申请人： Rajesh D. Patel , Nurith Kurn , Martin Becker , Edwin F. Ullman
发明人： Rajesh D. Patel , Nurith Kurn , Martin Becker , Edwin F. Ullman
IPC分类号： B01L3/00 , G01N37/00 , G01N1/10
CPC分类号： B01L3/5023 , B01L2300/0825 , B01L2400/0406 , B01L2400/0633 , B01L2400/0661 , B01L2400/0683 , Y10T436/2575
摘要： Methods and devices for permitting capillary flow of liquid between two or more pieces of bibulous material which, prior to actuation, are in a non-capillary flow relationship. In particular, the device is actuated and a capillary flow relationship is initiated between the two or more pieces of bibulous material in non-capillary flow relationship by utilizing a liquid expandable piece of bibulous material.
摘要翻译：用于允许两个或更多个吸水材料之间的液体的毛细管流动的方法和装置，其在致动之前处于非毛细管流动关系。特别地，该装置被致动，并且通过利用吸水材料的液体可膨胀件，以非毛细管流动关系在两个或多个吸管材料之间开始毛细管流动关系。

6. 发明授权

US4981786A Multiple port assay device 失效
标题翻译：多端口测定装置
公开(公告)号：US4981786A
公开(公告)日：1991-01-01
申请号：US094176
申请日：1987-09-04
申请人： Geoffrey A. Dafforn , Nurith Kurn , Martin Becker , Edwin F. Ullman
发明人： Geoffrey A. Dafforn , Nurith Kurn , Martin Becker , Edwin F. Ullman
IPC分类号： G01N33/53 , G01N33/543 , G01N33/558 , G01N37/00
CPC分类号： G01N33/558 , B01L3/5023 , G01N33/5302 , G01N33/54366 , Y10S435/805 , Y10S435/81 , Y10S436/81
摘要： A device is disclosed for conducting an assay. The device comprises a housing, means enclosed in the housing for capturing a first member of a specific binding pair (sbp) in a zone and for allowing liquid to be transported by capillary action away from the zone, first means in the housing for introducing the sample into the device, and second means in the housing other than the first means for introducing a liquid reagent other than the sample into the device. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an assay are also disclosed.

7. 发明授权

US4868104A Homogeneous assay for specific polynucleotides 失效
标题翻译：特异性多核苷酸的均相测定
公开(公告)号：US4868104A
公开(公告)日：1989-09-19
申请号：US773386
申请日：1985-09-06
申请人： Nurith Kurn , Chander Bahl , Edwin F. Ullman
发明人： Nurith Kurn , Chander Bahl , Edwin F. Ullman
IPC分类号： G01N33/53 , C12Q1/00 , C12Q1/68 , C12Q1/70 , G01N33/50 , G01N33/58 , G01N33/60
CPC分类号： C12Q1/705 , C12Q1/6813 , C12Q1/6832 , Y10S435/81 , Y10S436/808
摘要： A method for determining the presence of a polynucleotide analyte in a sample suspected of containing the analyte is disclosed. The method comprises combining in an assay medium the sample and first and second polynucleotide reagents complementary to the analyte. Each of the first and second reagents hybridize with a different region of the analyte. The first reagent contains means for rendering the first reagent non-covalently polymerizable. The second reagent contains means for rendering the second reagent detectable. The sample and the first and second reagents are combined in the assay medium under conditions for polymerizing the first reagent wherein the second reagent becomes bound to the polymerized first reagent only when the analyte is present in the sample. A determination is then made as to whether the second reagent has become bound to the polymerized first reagent. The method has broad application for determining the presence of a polynucleotide analyte such as DNA, RNA, the genomes of viruses, bacteria, molds, fungi, and fragments thereof, and the like. Preferred means for rendering the first reagent non-covalently polymerizable includes a repeating oligonucleotide sequence covalently bound to the first reagent.
摘要翻译：公开了一种用于确定怀疑含有分析物的样品中多核苷酸分析物的存在的方法。该方法包括在测定培养基中混合样品和与分析物互补的第一和第二多核苷酸试剂。第一和第二试剂中的每一个与分析物的不同区域杂交。第一试剂含有使第一试剂非共价可聚合的方法。第二试剂含有用于使第二试剂可检测的装置。样品和第一试剂和第二试剂在测定培养基中合并的条件下，只有当分析物存在于样品中时，第一试剂的第一试剂的聚合条件是第二试剂与聚合的第一试剂结合。然后确定第二试剂是否已经与聚合的第一试剂结合。该方法广泛用于确定多核苷酸分析物（例如DNA，RNA，病毒，细菌，霉菌，真菌及其片段的基因组）的存在等。使第一试剂非共价可聚合的优选方法包括与第一试剂共价结合的重复寡核苷酸序列。

8. 发明授权

US06555317B2 Detection of differences in nucleic acids 失效
公开(公告)号：US06555317B2
公开(公告)日：2003-04-29
申请号：US09732279
申请日：2000-12-07
申请人： Alla Lishanski , Nurith Kurn , Edwin F. Ullman
发明人： Alla Lishanski , Nurith Kurn , Edwin F. Ullman
IPC分类号： C12Q168
CPC分类号： C12Q1/6823 , C12Q1/6813 , C12Q1/6818 , C12Q1/6827 , Y10S435/808
摘要： A method is disclosed for detecting the presence of a difference between two related nucleic acid sequences. In the method a complex is formed comprising both strands of each sequence. Each member of at least one pair of non-complementary strands within the complex have labels. The association of the labels as part of the complex is determined as an indication of the presence of a difference between the two related sequences. The complex generally comprises a Holliday junction. In one aspect a medium suspected of containing said two related nucleic acid sequences is treated to provide partial duplexes having non-complementary tailed portions at one end. The double stranded portions of the partial duplexes are identical except for said difference. One of the strands of one of the partial duplexes is complementary to one of the strands of the other of the partial duplexes and the other of the strands of one of the partial duplexes is complementary to the other of the strands of the other of the partial duplexes. The medium is subjected to conditions that permit the binding of the tailed portions of the partial duplexes to each other. If there is a difference in the related nucleic acid sequences, a stable complex is formed comprising a Holliday junction. If no difference exists, the complex dissociates into duplexes. A determination is made whether the stable complex is formed, the presence thereof indicating the presence of the related nucleic acid sequences. The method has application in detecting the presence of a mutation in a target sequence or in detecting the target sequence itself.

9. 发明授权

US5622870A Device and method for completing a fluidic circuit 失效
公开(公告)号：US5622870A
公开(公告)日：1997-04-22
申请号：US453860
申请日：1995-05-30
申请人： N. Chung Sizto , Nurith Kurn , Rajesh D. Patel , Martin Becker , Edwin F. Ullman
发明人： N. Chung Sizto , Nurith Kurn , Rajesh D. Patel , Martin Becker , Edwin F. Ullman
IPC分类号： B01L3/00 , B01L9/00 , G01N33/543 , G01N33/558 , G01N21/00
CPC分类号： G01N33/54366 , B01L3/5023 , B01L2300/0825 , B01L2300/087 , B01L2400/0406 , B01L2400/0633 , B01L9/52 , Y10S435/97 , Y10S435/975 , Y10S436/808 , Y10T436/2575
摘要： Disclosed are assay apparatus, devices and methods that permit longitudinal capillary flow of liquid between two pieces of bibulous material which, prior to actuation, are in a non-capillary flow relationship to each other. In particular, the devices utilize a distortable member which when distorted, actuates the device by creating a capillary flow relationship between the two pieces of bibulous material. In an alternative embodiment the apparatus, devices, and methods include at least one additional piece of bibulous material.

10. 发明授权

US5620657A Device and method for completing a fluidic circuit 失效
标题翻译：用于完成流体回路的装置和方法
公开(公告)号：US5620657A
公开(公告)日：1997-04-15
申请号：US453859
申请日：1995-05-30
申请人： N. Chung Sizto , Nurith Kurn , Rajesh D. Patel , Martin Becker , Edwin F. Ullman
发明人： N. Chung Sizto , Nurith Kurn , Rajesh D. Patel , Martin Becker , Edwin F. Ullman
IPC分类号： B01L3/00 , B01L9/00 , G01N33/543 , G01N33/558 , G01N21/00
CPC分类号： G01N33/54366 , B01L3/5023 , B01L2300/0825 , B01L2300/087 , B01L2400/0406 , B01L2400/0633 , B01L9/52 , Y10S435/97 , Y10S435/975 , Y10S436/808 , Y10T436/2575
摘要： Disclosed are assay apparatus, devices and methods that permit longitudinal capillary flow of liquid between two pieces of bibulous material which, prior to actuation, are in a non-capillary flow relationship to each other. In particular, the devices utilize a distortable member which when distorted, actuates the device by creating a capillary flow relationship between the two pieces of bibulous material. In an alternative embodiment the apparatus, devices, and methods include at least one additional piece of bibulous material.
摘要翻译：公开了允许两片吸水材料之间的液体的纵向毛细管流动的测定装置，装置和方法，其在致动之前彼此处于非毛细管流动关系。特别地，这些装置使用可变形构件，当变形时，通过在两片吸水材料之间产生毛细管流动关系来致动装置。在替代实施例中，装置，装置和方法包括至少一个额外的吸水材料。

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