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1. 发明授权

US5919641A Homogeneous immunoassays using enzyme inhibitors 失效
公开(公告)号：US5919641A
公开(公告)日：1999-07-06
申请号：US451326
申请日：1995-05-26
申请人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
发明人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
IPC分类号： C12Q1/34 , G01N33/531 , G01N33/535 , G01N33/542 , G01N33/58 , G01N33/53
CPC分类号： G01N33/542 , G01N33/581
摘要： A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described.

2. 发明授权

US5137808A Immunoassay device 失效
标题翻译：免疫测定装置
公开(公告)号：US5137808A
公开(公告)日：1992-08-11
申请号：US388176
申请日：1989-07-31
申请人： Edwin F. Ullman , Pyare Khanna , Rohan Peries
发明人： Edwin F. Ullman , Pyare Khanna , Rohan Peries
IPC分类号： G01N33/543 , G01N37/00
CPC分类号： G01N33/54386 , G01N33/54366 , Y10S435/975 , Y10S436/807 , Y10S436/81
摘要： A device is disclosed for conducting an assay method. The device comprises a housing, means enclosed in the housing for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action away from the zone, one or more self-contained liquid reagents enclosed in the housing for conducting an assay method for the determination of an analyte in the sample, and means in the housing for introducing the sample into the device. Preferably, the self-contained reagents are liquid reagents which are contained in a breakable container. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an assay method are also disclosed.
摘要翻译：公开了用于进行测定方法的装置。该装置包括壳体，封闭在壳体中的装置，用于捕获区域中的特定装订对的构件并允许液体通过毛细管作用远离区域输送，一个或多个包含在壳体中的独立液体试剂用于进行用于测定样品中的分析物的测定方法，以及用于将样品引入该装置的壳体中的装置。优选地，独立的试剂是容纳在易碎容器中的液体试剂。本发明的装置用于测定怀疑含有分析物的样品中的分析物的测定方法。还公开了用于进行测定方法的试剂盒。

3. 发明授权

US5972630A Homogeneous immunoassays using enzyme inhibitors 失效
标题翻译：使用酶抑制剂的均质免疫测定法
公开(公告)号：US5972630A
公开(公告)日：1999-10-26
申请号：US747082
申请日：1991-08-19
申请人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
发明人： Remy Cromer , Rohan Peries , Dariush Davalian , Carl N. Skold , Edwin F. Ullman , Kesavan Radika
IPC分类号： C12Q1/34 , G01N33/531 , G01N33/535 , G01N33/542 , G01N33/58 , C07K16/00 , C12Q1/00 , C12Q1/54
CPC分类号： G01N33/542 , G01N33/581
摘要： A method for determining the presence of an analyte which is a specific binding pair member in a sample suspected of containing the analyte is described. The method involves: bringing together in an aqueous medium, the sample, an enzyme bound to a first specific binding pair member and an inhibitor for the enzyme bound to a second specific binding pair member wherein each specific binding pair member is capable of binding to the analyte or to a specific binding pair member complementary to the analyte; analyzing the medium for the activity of said enzyme; and relating the activity to the amount of analyte present in the medium. Compositions of matter and kits are also described.
摘要翻译：描述了一种用于确定在怀疑含有分析物的样品中作为特异性结合对成员的分析物的存在的方法。该方法包括：在水性介质中将样品，与第一特异性结合对成员结合的酶和与第二特异性结合对成员结合的酶的抑制剂结合在一起，其中每个特异性结合对成员能够结合至分析物或与分析物互补的特异性结合对成员; 分析培养基中所述酶的活性; 并将活性与介质中存在的分析物的量相关联。还描述了物质和试剂盒的组成。

4. 发明授权

US4857453A Immunoassay device 失效
标题翻译：免疫测定装置
公开(公告)号：US4857453A
公开(公告)日：1989-08-15
申请号：US35562
申请日：1987-04-07
申请人： Edwin F. Ullman , Pyare Khanna , Rohan Peries
发明人： Edwin F. Ullman , Pyare Khanna , Rohan Peries
IPC分类号： G01N33/543 , G01N37/00
CPC分类号： G01N33/54366 , G01N33/54386 , Y10S435/805 , Y10S435/81 , Y10S436/807 , Y10S436/81
摘要： A device is disclosed for conducting an assay method. The device comprises a housing, means enclosed in the housing for capturing a member of a specific binding pair in a zone and for allowing liquid to be transported by capillary action away from the zone, one or more self-contained liquid reagents enclosed in the housing for conducting an assay method for the determination of an analyte in the sample, and means in the housing for introducing the sample into the device. Preferably, the self-contained reagents are liquid reagents which are contained in a breakable container. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an assay method are also disclosed.

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