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1. 发明申请

US20050069916A1 Ultra-sensitive detection systems 审中-公开
标题翻译：超敏感检测系统
公开(公告)号：US20050069916A1
公开(公告)日：2005-03-31
申请号：US10825568
申请日：2004-04-14
申请人： Brian Chait , Darin Latimer , Paul Lizardi , Eric Kershnar , Jon Morrow , Matthew Roth , Martin Mattessich , Kevin McConnell
发明人： Brian Chait , Darin Latimer , Paul Lizardi , Eric Kershnar , Jon Morrow , Matthew Roth , Martin Mattessich , Kevin McConnell
IPC分类号： G01N27/62 , C07H21/02 , C07H21/04 , C07K1/04 , C07K1/107 , C07K1/13 , C07K7/00 , C07K7/06 , C07K7/08 , C12Q1/68 , G01N27/333 , G01N27/447 , G01N27/64 , G01N33/483 , G01N33/53 , G01N33/566 , G01N33/58 , G01N33/68
CPC分类号： G01N33/6842 , C07K1/047 , C07K1/1077 , C07K1/13 , C07K7/06 , C07K7/08 , G01N33/6803
摘要： Disclosed are compositions and methods for sensitive detection of one or multiple analytes. In general, the methods involve the use of special label components, referred to as reporter signals, that can be associated with, incorporated into, or otherwise linked to the analytes. In some embodiments, the reporter signals can be altered such that the altered forms of different reporter signals can be distinguished from each other. In some embodiments, sets of reporter signals can be used where two or more of the reporter signals in a set have one or more common properties that allow the reporter signals having the common property to be distinguished and/or separated from other molecules lacking the common property. In other embodiments, sets of reporter signal/analyte conjugates can be used where two or more of the reporter signal/analyte conjugates in a set have one or more common properties that allow the reporter signal/analyte conjugates having the common property to be distinguished and/or separated form other molecules lacking the common property. Reporter signals can also be in conjunction with analytes (such as in mixtures of reporter signals and analytes), where no significant physical association between the reporter signals and analytes occurs; or alone, where no analyte is present.
摘要翻译：公开了用于敏感检测一种或多种分析物的组合物和方法。通常，这些方法涉及使用称为报告信号的特殊标记成分，其可以与分析物相关联，并入或以其它方式连接到分析物上。在一些实施方案中，可以改变报道信号，使得不同报道信号的改变形式可以彼此区分。在一些实施方案中，可以使用一组报告信号，其中一组中的两个或更多个报告基因信号具有一个或多个共同性质，其允许具有共同性质的报道信号被区分和/或与其他分子不相同属性。在其它实施方案中，可以使用组的报告信号/分析物缀合物，其中组中的两个或多个报道信号/分析物缀合物具有一个或多个共同性质，其允许区分具有共同性质的报道信号/分析物缀合物， /或分离形成其他缺乏共同性质的分子。记者信号也可以与分析物（例如报告信号和分析物的混合物）结合，其中报告信号和分析物之间不存在明显的物理结合; 或单独存在，其中不存在分析物。

2. 发明授权

US06809318B2 Method of transmitting ions for mass spectroscopy 有权
公开(公告)号：US06809318B2
公开(公告)日：2004-10-26
申请号：US10657580
申请日：2003-09-08
申请人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
发明人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
IPC分类号： B01D5944
CPC分类号： H01J49/063 , H01J49/0481 , H01J49/161 , H01J49/164
摘要： A system for determining the ratio of mass to charge of an ion including a pulsed ionizer, a high pressure co-linear ion guide/accelerator, and a mass analyzer. The pulsed ionizer generates intact analyte ions from a sample of matter to be analyzed. The high pressure co-linear ion guide/accelerator is interfaced with the ion source for receipt of the intact ions of the sample. The ion guide/accelerator simultaneously dampens and linearly accelerates the intact ions in the substantial absence of fragmentation of the ions to provide a substantially continuous beam of the intact ions for mass analysis. The mass analyzer is connected to the ion guide/accelerator for receipt of the beam of ions and determines the mass to charge ratio of the intact ions.

3. 发明授权

US07109479B2 Method and system for mass spectroscopy 有权
公开(公告)号：US07109479B2
公开(公告)日：2006-09-19
申请号：US10952529
申请日：2004-09-28
申请人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
发明人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
IPC分类号： B01D59/44 , H01J49/00
CPC分类号： H01J49/063 , H01J49/0481 , H01J49/161 , H01J49/164
摘要： A system for determining the ratio of mass to charge of an ion including a pulsed ionizer, a high pressure co-linear ion guide/accelerator, and a mass analyzer. The pulsed ionizer generates intact analyte ions from a sample of matter to be analyzed. The high pressure co-linear ion guide/accelerator is interfaced with the ion source for receipt of the intact ions of the sample. The ion guide/accelerator simultaneously dampens and linearly accelerates the intact ions in the substantial absence of fragmentation of the ions to provide a substantially continuous beam of the intact ions for mass analysis. The mass analyzer is connected to the ion guide/accelerator for receipt of the beam of ions and determines the mass to charge ratio of the intact ions.

4. 发明授权

US07012249B2 High capacity and scanning speed system for sample handling and analysis 失效
公开(公告)号：US07012249B2
公开(公告)日：2006-03-14
申请号：US10924269
申请日：2004-08-23
申请人： Andrew Krutchinsky , Brian Chait
发明人： Andrew Krutchinsky , Brian Chait
IPC分类号： B01D59/44
CPC分类号： G01N21/253 , B82Y15/00 , G01N35/00029 , G01N35/00069 , G01N2035/00158 , G01N2035/1041
摘要： Disclosed is an apparatus for examining and inspecting at least one sample in order to determine characteristics of the sample having the a support for receiving a compact disc, the compact disc having deposited on a surface thereof at least one sample, inspection means for effecting a physical change in at least one sample, the inspection means positioned for registration with the surface of the compact disc bearing at least one sample; and a traversal mechanism adapted for reciprocating movement, to move the sample in and out of the path of the inspection means.

5. 发明申请

US20050153454A1 Method for detecting post-translational modification of peptides 审中-公开
标题翻译：检测肽的翻译后修饰的方法
公开(公告)号：US20050153454A1
公开(公告)日：2005-07-14
申请号：US11001908
申请日：2004-12-01
申请人： Brian Chait , Ronald Beavis , Rong Wang , Stephen Kent
发明人： Brian Chait , Ronald Beavis , Rong Wang , Stephen Kent
IPC分类号： C07K1/107 , C07K1/12 , G01N33/68 , G01N33/00
CPC分类号： G01N33/6842 , C07K1/107 , C07K1/128 , G01N33/6824 , Y10T436/24 , Y10T436/25 , Y10T436/255
摘要： Method is described for sequencing polypeptides by forming peptide ladders comprising a series of polypeptides in which adjacent members of the series vary by one amino acid residue and determining the identity and position of each amino acid in the polypeptide by mass spectroscopy.
摘要翻译：描述了通过形成包含一系列多肽的肽梯级来测序多肽的方法，其中该序列的相邻成员变化一个氨基酸残基，并通过质谱确定多肽中每个氨基酸的同一性和位置。

6. 发明申请

US20050077460A1 High capacity and scanning speed system for sample handling and analysis 失效
标题翻译：高容量和扫描速度系统，用于样品处理和分析
公开(公告)号：US20050077460A1
公开(公告)日：2005-04-14
申请号：US10924269
申请日：2004-08-23
申请人： Andrew Krutchinsky , Brian Chait
发明人： Andrew Krutchinsky , Brian Chait
IPC分类号： G01Q60/00 , G01N21/25 , G01N35/00 , G01N35/10 , G01Q30/02 , H01J49/00
CPC分类号： G01N21/253 , B82Y15/00 , G01N35/00029 , G01N35/00069 , G01N2035/00158 , G01N2035/1041
摘要： Disclosed is an apparatus for examining and inspecting at least one sample in order to determine characteristics of the sample having the a support for receiving a compact disc, the compact disc having deposited on a surface thereof at least one sample, inspection means for effecting a physical change in at least one sample, the inspection means positioned for registration with the surface of the compact disc bearing at least one sample; and a traversal mechanism adapted for reciprocating movement, to move the sample in and out of the path of the inspection means.
摘要翻译：公开了一种用于检查和检查至少一个样品的装置，以便确定具有用于接收光盘的支撑件的样品的特性，该光盘在其表面上沉积有至少一个样品，检查装置用于实现物理改变至少一个样品，所述检查装置定位成与承载至少一个样品的所述压片的表面对准; 以及适于往复运动的横移机构，以使样本进出检查装置的路径。

7. 发明申请

US20070077600A1 Methods and kits for distinguishing between specific and non-specific protein associations 有权
标题翻译：用于区分特异性和非特异性蛋白质关联的方法和试剂盒
公开(公告)号：US20070077600A1
公开(公告)日：2007-04-05
申请号：US11508736
申请日：2006-08-21
申请人： Brian Chait , Alan Tackett , Jeffrey DeGrasse , Marlene Oeffinger , Michael Rout , Matthew Sekedat
发明人： Brian Chait , Alan Tackett , Jeffrey DeGrasse , Marlene Oeffinger , Michael Rout , Matthew Sekedat
IPC分类号： G01N33/567 , G01N33/574 , G01N33/569 , G01N33/554 , G01N33/53
CPC分类号： G01N33/5023 , G01N33/5032 , G01N33/60 , G01N33/6845 , G01N33/6848
摘要： The present invention is method of determining whether or not associations between a given protein and other proteins in a cell are specific. The method comprises (a) providing a first sample of the cells in which the given protein contains a tag, (b) providing a second sample of the same cells, wherein the given protein and the other proteins are metabolically labeled, and wherein neither the given protein nor the other proteins are tagged, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (i) proteins specifically associated with the tagged given protein, (ii) proteins non-specifically associated with the tagged given protein, or (iii) a combination thereof, (f) determining whether each associated protein is unlabeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is not labeled, then that protein was specifically associated in the cell with the tagged given protein.
摘要翻译：本发明是确定细胞中给定蛋白质和其它蛋白质之间的缔合是否是特异性的方法。该方法包括（a）提供其中给定蛋白质含有标签的细胞的第一样品，（b）提供相同细胞的第二样品，其中所述给定蛋白质和其它蛋白质被代谢标记，并且其中（c）混合和裂解第一细胞样品和第二细胞样品以提供蛋白质的混合物，（d）将给定蛋白质的标签与底物结合，（e）分离蛋白质与标记的给定蛋白质结合至底物，其中相关蛋白质包含：（i）与标记的给定蛋白质特异性相关的蛋白质，（ii）与标记的给定蛋白质非特异性相关的蛋白质，或（iii）其组合，（f）确定每个相关蛋白是否未标记，或标记蛋白和未标记蛋白质的混合物，其中如果相关蛋白未被标记，那么该蛋白质与标记特异性结合在细胞中给定蛋白质。

8. 发明申请

US20070045533A1 Novel linear ion trap for mass spectrometry 有权
标题翻译：用于质谱的新型线性离子阱
公开(公告)号：US20070045533A1
公开(公告)日：2007-03-01
申请号：US11216459
申请日：2005-08-31
申请人： Andrew Krutchinsky , Herbert Cohen , Brian Chait
发明人： Andrew Krutchinsky , Herbert Cohen , Brian Chait
IPC分类号： B01D59/44
CPC分类号： H01J49/423
摘要： A method for manipulating ions in an ion trap includes storing ions, spatially compressing, and ejecting selected ions according to mass-to-charge ratio. An ion trap includes an injection port, an arm having a first and a second end for confining and spatially compressing the ions, and an ejection port for ejecting the ions from the second end. The arm includes two pairs of opposing electrodes, which provide a quadrupole electric field potential at any cross-section of the ion trap. The distance between opposing electrodes and the cross-sectional area of the electrodes increases from the first to second end. The electrodes may be tapered cylindrical rods or of hyperbolic cross-section. Ions selected for ejection are spatially compressed into a region at the second (wider) end. The ion trap may include one arm, with either orthogonal or axial ejection, or two arms with a central insert for orthogonal ejection.
摘要翻译：用于操作离子阱中的离子的方法包括按照质荷比来存储离子，空间压缩和排出选定的离子。离子阱包括注入口，具有用于限制和空间压缩离子的第一和第二端的臂以及用于从第二端喷出离子的排出口。臂包括两对相对的电极，其在离子阱的任何横截面处提供四极电场电位。相对电极之间的距离和电极的横截面面积从第一到第二端增加。电极可以是锥形圆柱形杆或双曲线截面。选择用于喷射的离子被空间压缩到第二（较宽）端的区域。离子阱可以包括具有正交或轴向排出的一个臂或具有用于正交喷射的中心插入件的两个臂。

9. 发明申请

US20050092912A1 Method and system for mass spectroscopy 有权
标题翻译：质谱方法和系统
公开(公告)号：US20050092912A1
公开(公告)日：2005-05-05
申请号：US10952529
申请日：2004-09-28
申请人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
发明人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
IPC分类号： H01J49/16 , H01J49/42 , H01J49/00 , B01D59/44 , H01J49/40
CPC分类号： H01J49/063 , H01J49/0481 , H01J49/161 , H01J49/164
摘要： A system for determining the ratio of mass to charge of an ion including a pulsed ionizer, a high pressure co-linear ion guide/accelerator, and a mass analyzer. The pulsed ionizer generates intact analyte ions from a sample of matter to be analyzed. The high pressure co-linear ion guide/accelerator is interfaced with the ion source for receipt of the intact ions of the sample. The ion guide/accelerator simultaneously dampens and linearly accelerates the intact ions in the substantial absence of fragmentation of the ions to provide a substantially continuous beam of the intact ions for mass analysis. The mass analyzer is connected to the ion guide/accelerator for receipt of the beam of ions and determines the mass to charge ratio of the intact ions.
摘要翻译：用于确定包括脉冲离子发生器，高压共线性离子导向器/加速器和质量分析器的离子的质量与电荷的比率的系统。脉冲电离器从待分析的物质样品中产生完整的分析物离子。高压共线离子导向器/加速器与离子源接口以接收样品的完整离子。离子导向器/加速器在基本上不存在离子碎裂的同时抑制和线性加速完整离子，以提供基本上连续的完整离子束用于质量分析。质量分析器连接到离子导向器/加速器以接收离子束，并确定完整离子的质荷比。

10. 发明授权

US06617577B2 Method and system for mass spectroscopy 有权
公开(公告)号：US06617577B2
公开(公告)日：2003-09-09
申请号：US09835943
申请日：2001-04-16
申请人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
发明人： Andrew Krutchinsky , Herbert Cohen , Markus Kalkum , Vadim Sherman , Brian Chait
IPC分类号： B01D5944
CPC分类号： H01J49/063 , H01J49/0481 , H01J49/161 , H01J49/164
摘要： A system for determining the ratio of mass to charge of an ion including a pulsed ionizer, a high pressure co-linear ion guide/accelerator, and a mass analyzer. The pulsed ionizer generates intact analyte ions from a sample of matter to be analyzed. The high pressure co-linear ion guide/accelerator is interfaced with the ion source for receipt of the intact ions of the sample. The ion guide/accelerator simultaneously dampens and linearly accelerates the intact ions in the substantial absence of fragmentation of the ions to provide a substantially continuous beam of the intact ions for mass analysis. The mass analyzer is connected to the ion guide/accelerator for receipt of the beam of ions and determines the mass to charge ratio of the intact ions.

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