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1. 发明申请

US20070141673A1 Tool for quantitative real-time analysis of viral gene expression dynamics in single living cells 审中-公开
标题翻译：用于定量实时分析单个活细胞中病毒基因表达动力学的工具
公开(公告)号：US20070141673A1
公开(公告)日：2007-06-21
申请号：US11602487
申请日：2006-11-21
申请人： Asier Saez-Cirion , Marie-Anne Nicola , Gianfranco Pancino , Spencer Shorte
发明人： Asier Saez-Cirion , Marie-Anne Nicola , Gianfranco Pancino , Spencer Shorte
IPC分类号： C12Q1/68 , C12P21/06
CPC分类号： C12Q1/70 , C12Q1/6897 , C12Q1/701 , C12Q1/703 , C12Q2565/601 , C12Q2563/103 , C12Q2561/113
摘要： The invention provides a method allowing the detection and the quantitative real-time measurement, at the single living cell level, of viral replication using a bioluminescent reporter gene and a digital light detection device sensitive to detect single photons with high efficiency and assign them a lateral x,y coordinate and precise temporal incidence (i.e. a “time point”), wherein the spatial and temporal characteristics of bioluminescence is indicative at the single living cell level of viral replication.
摘要翻译：本发明提供了一种使用生物发光报告基因在单个活细胞水平上进行病毒复制的检测和定量实时测量的方法，并且能够高效检测单个光子的敏感的数字光检测装置， x，y坐标和精确的时间发生率（即“时间点”），其中生物发光的空间和时间特征指示病毒复制的单个活细胞水平。

2. 发明申请

US20100309304A1 Method and Device for the Reconstruction of the Shape of an Object from a Sequence of Sectional Images of Said Object 审中-公开
标题翻译：用于根据所述对象的截面图像的序列重建对象的形状的方法和装置
公开(公告)号：US20100309304A1
公开(公告)日：2010-12-09
申请号：US12739006
申请日：2008-10-22
申请人： Bernard Chalmond , Alain Trouve , Yong Yu , Jiaping Wang , Olivier Renaud , Spencer Shorte
发明人： Bernard Chalmond , Alain Trouve , Yong Yu , Jiaping Wang , Olivier Renaud , Spencer Shorte
IPC分类号： H04N7/18 , G06K9/00
CPC分类号： G02B21/008 , G06T7/571 , G06T7/70 , G06T15/00 , G06T2200/04 , G06T2207/10056 , G06T2207/30024
摘要： A method of reconstructing the volume of an object from a sequence of section images, the images corresponding to different positions and/or orientations of an acquisition plane and being subject to uncertainties, the method comprising: a) selecting a finite base of functions on which the volume for reconstruction can be decomposed; b) selecting a first quantification function for quantizing the difference between the real position and/or orientation of each section relative to said object and its nominal position and/or orientation; c) selecting a second quantification function for quantizing the spatial coherence of the reconstructed volume; d) selecting a third quantification function for quantizing the difference between the section images of the object and the corresponding sections of the reconstructed volume; e) selecting an overall cost function, of value that depends on the values of said first, second, and third quantizing functions; and f) jointly estimating the real positions and/or orientations of the section, together with the coefficient for decomposing the image of the object on said function base, by minimizing said overall cost function.
摘要翻译：一种从部分图像序列重建物体的体积的方法，所述图像对应于采集平面的不同位置和/或取向并且受到不确定性的影响，所述方法包括：a）选择有限基础的函数，重建体积可以分解; b）选择用于量化每个部分相对于所述物体的实际位置和/或取向与其标称位置和/或取向之间的差的第一量化功能; c）选择量化所述重建体积的空间相干性的第二量化函数; d）选择用于量化对象的截面图像和重构体积的相应部分之间的差的第三量化功能; e）选择取决于所述第一，第二和第三量化函数的值的总成本函数; 以及f）通过使所述总体成本函数最小化来共同估计所述部分的实际位置和/或取向以及用于分解所述功能基础上的对象的图像的系数。

3. 发明申请

US20130115647A1 Method to Increase the Number of Detectable Photons During the Imaging of a Biological Marker 有权
标题翻译：在生物标记成像期间增加可检测光子数量的方法
公开(公告)号：US20130115647A1
公开(公告)日：2013-05-09
申请号：US13637551
申请日：2011-03-25
申请人： Kelly Rogers , Spencer Shorte , Joseph Dragavon , Samantha Blazquez
发明人： Kelly Rogers , Spencer Shorte , Joseph Dragavon , Samantha Blazquez
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , B82Y15/00 , G01N21/6428 , G01N2201/06193
摘要： The present invention relates a method to determine the presence of a photon producing biological marker in a cell, tissue or organism of interest. The method is based on Fluorescence by Unbound Excitation from Luminescence (FUEL) and comprises the steps of a) providing conditions suitable for the biological marker to produce at least one first photon by luminescence; b) providing a FUEL probe pair-upper (FPP-U) disposed in proximity to the cell, tissue or organism, wherein the at least one first photon of step a) excites the FPP-U, which emits at least one second photon. The FPP-U may be selected from the group of quantum dots, carbon nanotubes, fluorescent proteins, diamond nanocrystals and metalloporphyrins. This method is charcterized in that said biological marker and said FPP-U are not bound and in that each of the at least one second photon(s) are of a longer wavelength than each of the at least one first photon(s).
摘要翻译：本发明涉及确定感兴趣的细胞，组织或生物体中产生光子的生物标志物的存在的方法。该方法基于通过未发光发光（FUEL）的荧光，并且包括以下步骤：a）提供适于生物标记物的条件以通过发光产生至少一个第一光子; b）提供设置在细胞，组织或生物体附近的FUEL探针对上（FPP-U），其中步骤a）的至少一个第一光子激发FPP-U，其发射至少一个第二光子。 FPP-U可以选自量子点，碳纳米管，荧光蛋白，金刚石纳米晶体和金属卟啉。该方法的特征在于所述生物标记和所述FPP-U不结合，并且所述至少一个第二光子中的每一个具有比所述至少一个第一光子中的每一个更长的波长。

4. 发明授权

US07738695B2 Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy 有权
标题翻译：用于提供高分辨率显微镜的悬浮微物体三维成像的方法和装置
公开(公告)号：US07738695B2
公开(公告)日：2010-06-15
申请号：US10532147
申请日：2003-10-24
申请人： Spencer Shorte , Torsten Müller , Thomas Schnelle
发明人： Spencer Shorte , Torsten Müller , Thomas Schnelle
IPC分类号： G06K9/00
CPC分类号： G02B21/22 , G01N2015/144 , G01N2015/1445 , G01N2015/1452 , G02B21/367
摘要： A method for high-resolution image recording of at least one object with a microscope, includes the steps of: (a) positioning the object in a receptacle being arranged in the optical axis of the microscope, (b) generating at least two first data sets per object which represent intermediate images of the object with at least two different orientations relative to the optical axis of the microscope, wherein the different orientations of the object are provided by moving the object relative to the receptacle, and (c) evaluating the data sets for obtaining quantitative three dimensional information.
摘要翻译：一种用显微镜对至少一个物体进行高分辨率图像记录的方法，包括以下步骤：（a）将物体放置在布置在显微镜的光轴上的容器中，（b）产生至少两个第一数据通过相对于显微镜的光轴具有至少两个不同取向来表示对象的每个对象的集合，其中通过相对于插座移动对象来提供对象的不同取向，以及（c）评估数据用于获得定量三维信息。

5. 发明授权

US09329132B2 Method to increase the number of detectable photons during the imaging of a biological marker 有权
标题翻译：在生物标记成像过程中增加可检测光子数量的方法
公开(公告)号：US09329132B2
公开(公告)日：2016-05-03
申请号：US13637551
申请日：2011-03-25
申请人： Kelly Rogers , Spencer Shorte , Joseph Dragavon , Samantha Blazquez
发明人： Kelly Rogers , Spencer Shorte , Joseph Dragavon , Samantha Blazquez
IPC分类号： G01N21/64 , B82Y15/00
CPC分类号： G01N21/6486 , B82Y15/00 , G01N21/6428 , G01N2201/06193
摘要： The present invention relates a method to determine the presence of a photon producing biological marker in a cell, tissue or organism of interest. The method is based on Fluorescence by Unbound Excitation from Luminescence (FUEL) and comprises the steps of a) providing conditions suitable for the biological marker to produce at least one first photon by luminescence; b) providing a FUEL probe pair-upper (FPP-U) disposed in proximity to the cell, tissue or organism, wherein the at least one first photon of step a) excites the FPP-U, which emits at least one second photon. The FPP-U may be selected from the group of quantum dots, carbon nanotubes, fluorescent proteins, diamond nanocrystals and metalloporphyrins. This method is characterized in that said biological marker and said FPP-U are not bound and in that each of the at least one second photon(s) are of a longer wavelength than each of the at least one first photon(s).
摘要翻译：本发明涉及确定感兴趣的细胞，组织或生物体中产生光子的生物标志物的存在的方法。该方法基于通过未发光发光（FUEL）的荧光，并且包括以下步骤：a）提供适于生物标记物的条件以通过发光产生至少一个第一光子; b）提供设置在细胞，组织或生物体附近的FUEL探针对上（FPP-U），其中步骤a）的至少一个第一光子激发FPP-U，其发射至少一个第二光子。 FPP-U可以选自量子点，碳纳米管，荧光蛋白，金刚石纳米晶体和金属卟啉。该方法的特征在于所述生物学标记和所述FPP-U不是结合的，并且所述至少一个第二光子中的每一个具有比所述至少一个第一光子中的每一个更长的波长。

6. 发明申请

US20060126921A1 Method and device for 3 dimensional imaging of suspended micro-objects providing high-resolution microscopy 有权
标题翻译：用于提供高分辨率显微镜的悬浮微物体三维成像的方法和装置
公开(公告)号：US20060126921A1
公开(公告)日：2006-06-15
申请号：US10532147
申请日：2003-10-24
申请人： Spencer Shorte , Torsten Muller , Thomas Schnelle
发明人： Spencer Shorte , Torsten Muller , Thomas Schnelle
IPC分类号： G06K9/00
CPC分类号： G02B21/22 , G01N2015/144 , G01N2015/1445 , G01N2015/1452 , G02B21/367
摘要： A method for high-resolution image recording of at least one object with a microscope, includes the steps of: (a) positioning the object in a receptacle being arranged in the optical axis of the microscope, (b) generating at least two first data sets per object which represent intermediate images of the object with at least two different orientations relative to the optical axis of the microscope, wherein the different orientations of the object are provided by moving the object relative to the receptacle, and (c) evaluating the data sets for obtaining quantitative three dimensional information.
摘要翻译：一种用显微镜对至少一个物体进行高分辨率图像记录的方法，包括以下步骤：（a）将物体放置在布置在显微镜的光轴上的容器中，（b）产生至少两个第一数据通过相对于显微镜的光轴具有至少两个不同取向来表示对象的每个对象的集合，其中通过相对于插座移动对象来提供对象的不同取向，以及（c）评估数据用于获得定量三维信息。

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