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    • 3. 发明授权
    • Phase-separation tube
    • 相分离管
    • US5389265A
    • 1995-02-14
    • US71137
    • 1993-06-02
    • Robert P. Luoma, II
    • Robert P. Luoma, II
    • G01N33/48B01D17/038B01D17/12B01L3/14B04B5/02G01N1/10G01N1/28G01N1/36G01N33/49
    • B01L3/5021G01N33/491B01L2300/0851Y10T436/111666
    • A tube and method of partitioning a pre-selected phase of a sample of liquid having a plurality of phases of differing densities. The tube has a smaller diameter at one end. In use, a sample of liquid is passed through a first end of a linear tube and into a first chamber of said tube. The first chamber is located at the first end of the tube and is separated from a second chamber located at a second opposed end of the tube by a separation device. The separation device slidably engages the interior surface of the tube in an essentially fluid-tight manner and has a flow-restriction orifice therein to permit fluid flow communication between the first and second chambers under the influence of force. The phases are then ordered within the tube using e.g. axial centrifugation. The volume of the first chamber is reduced by movement of the separation device within the tube, such that one phase of the liquid in the first chamber flows through the flow-restriction orifice and into the second chamber. The method is monitored using the section of the tube that has the smaller diameter.
    • 分配具有不同密度的多个相的液体样品的预选相的管和方法。 管在一端具有较小的直径。 在使用中,液体样品通过线性管的第一端并进入所述管的第一腔。 第一室位于管的第一端,并通过分离装置与位于管的第二相对端的第二室分离。 分离装置以基本上流体密封的方式可滑动地接合管的内表面,并且在其中具有限流孔,以允许在力的影响下第一和第二室之间的流体流动连通。 然后使用例如在管中排列相。 轴向离心。 通过分离装置在管内的移动来减小第一室的体积,使得第一室中的液体的一相流经流量限制孔并进入第二室。 使用具有较小直径的管的截面来监测该方法。
    • 4. 发明授权
    • Flow restrictor-separation device
    • 流量限制器分离装置
    • US5282981A
    • 1994-02-01
    • US877496
    • 1992-05-01
    • George A. AdamsRobert P. Luoma, II
    • George A. AdamsRobert P. Luoma, II
    • G01N33/48B01L3/14G01N33/49B01D21/26
    • G01N33/491B01L3/50215Y10T436/25375
    • A method of partitioning a pre-selected phase of a sample of liquid having a plurality of phases of differing densities, a separation device and a tube containing the separation device. The sample of liquid is placed in a first chamber of a linear tube that is separated from a second chamber by a separation device. The separation device slidably engages the interior surface of the tube in an essentially fluid-tight manner and has an axial orifice on the longitudinal axis of the tube that is in fluid flow communication with a flow-restriction channel. The phases are ordered concentrically by rotating the tube around its longitudinal axis e.g. in an axial centrifuge. The volume of the first chamber is reduced by movement of the separation device within the tube, that phase of the liquid located axially within the first chamber flowing into the axial orifice and passing through the flow-restriction channel into the second chamber. The reduction of the volume of the first chamber is controlled using phase-separation information. The method is useful in separation of blood components.
    • 分离具有不同密度的多个相的液体样品的预选相的方法,分离装置和含有分离装置的管。 将液体样品放置在通过分离装置与第二室隔开的直管的第一室中。 分离装置以基本上流体密封的方式可滑动地接合管的内表面,并且在管的纵向轴线上具有与流动限制通道流体流动连通的轴向孔口。 通过围绕其纵向轴旋转管,相位相同地排列,例如 在轴向离心机中。 通过分离装置在管内的移动来减小第一室的体积,液体的轴向位于第一室内的液相流入轴向孔口并通过流动限制通道进入第二室。 使用相分离信息来控制第一室的体积的减小。 该方法可用于分离血液成分。
    • 5. 发明授权
    • Centrifugal methods using a phase-separation tube
    • 使用相分离管的离心方法
    • US5271852A
    • 1993-12-21
    • US877497
    • 1992-05-01
    • Robert P. Luoma, II
    • Robert P. Luoma, II
    • G01N33/48B01L3/14G01N33/49B01D21/26
    • G01N33/491B01L3/50215Y10T436/25375
    • A method of partitioning a pre-selected phase of a sample of liquid having a plurality of phases of differing densities, and a tube. A sample of liquid is passed through a first end of a linear tube and into a first chamber of said tube. The first chamber is located at the first end of the tube and is separated from a second chamber located at a second opposed end of the tube by a separation device. The separation device slidably engages the interior surface of the tube in an essentially fluid-tight manner and has a flow-restriction orifice therein to permit fluid flow communication between the first and second chambers under the influence of force. The phases are then ordered within the tube using e.g. axial centrifugation. The volume of the first chamber is reduced by movement of the separation device within the tube, such that one phase of the liquid in the first chamber flows through the flow-restriction orifice and into the second chamber.
    • 分离具有不同密度的多个相的液体样品的预选相的方法和管。 将液体样品通过线性管的第一端并进入所述管的第一腔。 第一室位于管的第一端,并通过分离装置与位于管的第二相对端的第二室分离。 分离装置以基本上流体密封的方式可滑动地接合管的内表面,并且在其中具有限流孔,以允许在力的影响下第一和第二室之间的流体流动连通。 然后使用例如在管中排列相。 轴向离心。 通过分离装置在管内的移动来减小第一室的体积,使得第一室中的液体的一相流经流量限制孔并进入第二室。